China ; Humans ; Mouth Diseases ; Mouth Mucosa

Administration, Topical ; Humans ; Immunosuppressive Agents ; administration & dosage ; adverse effects ; therapeutic use ; Lichen Planus, Oral ; complications ; drug therapy ; pathology ; Oral Ulcer ; drug therapy ; etiology ; Tacrolimus ; administration & dosage ; adverse effects ; therapeutic use ; Treatment Outcome

The principle of the relative operating characteristic (ROC) curve, along with constructing curve, a cutoff value between therapeutic and non-therapeutic concentration, formula for calculation, and statistic analysis, was introduced. Illustrated by an example, ROC was an important tool in analysis of the fixed effect model. It can beapplied to determine the relationship between a blood concentration and its action, probability of certain concentration producing therapeutic action, and high or low limit of therapeutic window, etc.

Alzheimers disease (AD) is a progressively and retrogressively neurodegenerative disorder of central nervous system severely threatening the aged. The studies of animal models, which share the very similar neuropathological characteristics with human, provide tools for the understanding of the etiology, mechanisms and medicine screening of AD. The distribution and chemical composition of amyloid-beta (A- beta) peptide -positive deposits were investigated in various non-human primate models. Various proportions of A- beta deposits contained cholinesterase activities, apolipoprotein E(ApoE) and alpha1-antichymotrypsin immunoreactivity. Alterations in the basal forebrain cholinergic system have been widely studied, but the magnitude of decline and relationship to cognitive impairment are still a matter of debate. This article also includes the latest research of the changing on tau, subtle structural alterations in axons, and neurofibrillary tangles (NFT) in the brain tissues of the non-human primate models.

Objective To investigate the clinical characteristics of clavicular defect and nonunion after operation and evaluate the clinical effects of vascularized pedicled pectoralis major clavicular periosteal flap and locking plate fixation plus bone morphogenetic protein (BMP) implantation. Methods From January 2004 to April 2008, 12 patients (mean age, 42.8 years old) with clavicular defect and nonunion were treated in Zhongshan Hospital. The internal fixators were removed, and then fibrous scars and sclerotic bones were cleared. The clavicular detects were fixed with locking plate, with implantation of the BMP. The wound was covered with vascularized pedicled pectoralis major clavicular periosteal flap. Postoperative functional rehabilitation exercises were performed. Results All patients were followed up for 8-24 months (mean 1.2 years). The clinical results were evaluated by Constant-Murley scoring system. The clavicular defects were healed in all patients within 4-7 months, and the internal fixators were successfully removed from some of the patients. Conclusions Vascularized pedicled pectoralis major clavicular periosteal flap combined with locking plate fixation plus BMP implantation is effective in the treatment of clavicular defect and nonunion.

Cord blood is the main source of stem cell outside of bone marrow and peripheral blood, and cord blood transplantation, especially unrelated cord blood transplantation (UCBT) has been widely used. The major challenges in UCBT are delayed engraftment and graft rejection. Many approaches have been investigated to enhance collection of HSC and HPC in cord blood units,sinduding use of double unit UCBT, adopt reduced intensity conditioning (RIC) approach, amplification of cord blood cells in vivo or ex vivo, and co-infusion with a haploidentical T cell depleted graft or mesenchymal stem cells(MSC),or injecting cord blood cells directly into the bone marrow.Those approaches may greatly increase the clinical use of cord blood cells for transplantation.

A new closed bipolar electrode electrochemiluminescence ( ECL)-based device was designed, and further used to investigate the ECL behaviors of luminol in this device. Our results showed that, while a suitable voltage was applied to the two poles of the closed bipolar electrode, both the positively charged ions and luminol-based anionic ions could be enriched on the two poles of the closed bipolar electrode, respectively. More importantly, the ECL signals, generated from the electro-oxidation of luminol on anodic pole, were found to be related to the total amount of positively charged ions on the cathodic pole of the closed bipolar electrode. Under the optimum experimental conditions, the ECL response was linearly to the concentration of analyte in the range of 1. 0×10-9-1. 0×10-8 mol/L with a detecting limit of 1. 1×10-10 mol/L. Based on this finding, a new ECL method for sensing the solution conductance was developed.

Objective To discuss and analyze clinical pathological characteristics of senile recurrent ovarian cancer and its influences on prognosis.Methods 140 senile ovarian cancer patients who have been admitted in ourhospital were selected as study subjects.103 cases with recurrent ovarian cancer were selected as observation group (group A),and the rest 37 cases who did not suffer from recurrent ovarian cancer were selected as control group(group B).Pathological characteristics of carcinoma tissues after their first admission in our hospital were compared between the two groups.And pathological characteristics of carcinoma tissues of group A after their second admissionin our hospital were analyzed,comprehensively analyzed prognosis situations and clinical pathological characteristics of patients in these two groups.Results In group A,the number of poorly differentiated carcinoma tissues was obviouslymore than group B,and the number of patients whose neoplasm staging was M stage was more than that in group B,the differences between the two groups were significant (x2 =13.48,15.87,all P ＜ 0.05).The incidence rate of adverse reactions between the two groups also had statistical significance(x2 =13.8,11.6,14.5,13.9,14.9,13.4,all P ＜0.05).Conclusion The clinical pathological characteristics of senile recurrent ovarian cancer can reflect patients'prognosis situations in some degree,and has certain clinical value in evaluation of patients' disease recurrence and prognosis.

Objective To observe the expression of phosphorylation of signal transducer and activa -tor of transcription 5 (STAT5) protein and the DNA-binding activity of STAT5 after interleukin 2 therapy for genital herpes .Methods Six patients with recurrent genital herpes were included in this study .CD4 +T cells from patients who underwent interleukin 2 therapy were analyzed for changes in STAT 5 signaling.None had been treated with corticosteroid and immunosuppressive agents .Phosphorylation of STAT 5 was detected in the T cells using Western blot analysis .Electrophoretic mobility shift assay ( EMSA) was carried out to detect the DNA-biding activity of STAT5.Results The expressions of phosphorylated STAT 5 in T cells de-rived from patients with genital herpes 28 days after interleukin treatment were 1.8~2.7 fold to that of be-fore the treatment was given .STAT5 DNA-binding activity in T cells derived form patients with genital her-pes who had received the treatment was 2.3~3.4 fold increased compared to that of before the initiating of interleukin 2 treatment.The differences between the before and after interleukin 2 treatment were statistical-ly significant( t =10.6, P <0.05).Conclusion This study indicates that STAT5 may be an important signaling mediator of interleukin 2 therapies , and that STAT5 activation may predict response to this treat-ment .

Objective To understand adolescent's mental stress, coping style and their correlating with emotion. Methods This research, in order to be tried with 5870 middle school students, adopt Mental Stress Questionnaire, Coping Style Scale and Profile of Mood States. Results Compared with male students, female students had higher scores on stress from school work achievement and appearance(P＜0.05) and lower scores on stress from family[(2.04±1.19)vs(2.11±1.25)](P＜0.05),also they had higher sores on extra-coping style[(3.11±0.77)vs(2.77±0.81)](P＜0.01). Compared with lower grade students, higher grade students had higher scores on all mental stress except stress from family, while they had lower scores on extra-coping style (P＜0.01). Stepwise logistic regression analysis indicated that high scores on mental stress and intro-coping style could predict negative emotion (P＜0.01), and extra-coping style could predict positive emotion (P＜0.01).Conclusion The middle school students suffered more stress from school work achievement than from other aspects. Students who differed in sex, grade had significant different scores on mental stress and coping style. Mental stress and coping style could predict adolescents' emotion.