1.Comparison of Direct-labeling Method of Antibody with 99mTc and 188Re.
Chang Woon CHOI ; Sang Moo LIM ; Kwang Sun WOO ; Wee Sup CHUNG ; Tae Hyun CHOI ; Soo Jeong LIM
Korean Journal of Nuclear Medicine 1999;33(1):84-93
PURPOSE: We investigated the direct labeling method of antibody with 99mTc and 188Re and examined the stability and function of these labeled compounds in in vitro and in vivo. MATERIALS AND METHODS: Disulfide bond of nonspecific human IgG was reduced to -SH group by 2-mercaptoethanol. Stannous ion was used to reduce 99mTc and 188Re. The stability of 99mTc-IgG and 188Re-IgG was estimated upto 24 hrs. Biodistribution was evaluated in abscess bearing rats at 4 and 24 hr post-injection of 99mTc or 188Re labeled IgG. RESULTS: The number of -SH group per reduced IgG molecule was 2.34. The labeling yield of 99mTc-IgG and 188Re-IgG were 90% and 95%, respectively. The stability of 99mTc-IgG at 1, 4, 6 and 24 hr was 91%, 83%, 78%, 7% and that of 188Re-IgG, high uptake was found on kidney, blood, stomach and abscess (9.42+/-0.68, 1.43+/-0.24, 0.86+/-0.18, 0.72+/-0.10 %ID/g, respectively). The uptakes at 24 hr were kidney, abscess, stomach, and blood in descending order. In case of 188Re-IgG, high uptake at 4 hr post injection appeared on kidney, blood, abscess and stomach (3.92+/-0.62, 1.32+/-0.08, 0.88+/-0.01, 0.26+/-0.06, respectively). The upatkes at 24 hr were kidney, abscess, blood abd stomach in descending order. The abscess to blood uptake ratio of 99mTc-IgG was 0.5 at 4 hr and 2.02 at 24 hr and that of 188Re-IgG was 0.67 and 1.29. CONCLUSION: 99mTc-IgG and 188Re-IgG and 188Re-IgG canbe labeled efficiently with direct labeling method. However, 99mTc-IgG and 188Re-IgG, labeled with direct method, was unstable. Further study in needed to enhance the stability of the antibody labeling.
Abscess
;
Animals
;
Humans
;
Immunoglobulin G
;
Kidney
;
Mercaptoethanol
;
Rats
;
Stomach
2.Polymer formation and altered biodistribution of IgG labelled with Tc and cyclic DTPA.
Sang Moo LIM ; Kwang Sun WOO ; Wee Sup CHUNG ; Ok Doo AWH
Korean Journal of Nuclear Medicine 1993;27(2):270-276
No abstract available.
Immunoglobulin G*
;
Pentetic Acid*
;
Polymers*
3.Calcification within primary lung cancer in Korea.
Woo Sun KIM ; Tae Hwan LIM ; Kwang Gil PARK ; Young Kuk CHO ; Seung Yon BEAK
Journal of the Korean Radiological Society 1991;27(1):71-76
No abstract available.
Korea*
;
Lung Neoplasms*
;
Lung*
4.Vestibuloneuritis Developed Concurrently in Ipsilateral Site with Herpes-Zoster Oticus Syndrome .
Gyu Cheol HAN ; Ju Hyoung LEE ; Joo Hyun WOO ; Jung Kook YOO ; Sun Hwa LIM
Journal of the Korean Balance Society 2004;3(1):187-191
BACKGROUND:Acute vestibular neuronitis is the disease of which the etiology and pathophysiology are largely unknown . But the viral infection and ischemia of the labyrinth and the vestibular nerve are considered as general etiology. This study was performed to support the viral infection rather than the ischemic theory. MATERIALS & METHODS:We studied seventy years old female patient who showed painful vesicles on left auricle and vertigo with spontaneous nystagmus to the right side. We performed physical examination, serologic test, ENG test, pure tone audiogram, brain magnetic resonance imaging and polymerase chain reaction. RESULTS:We found small vesicles and vascular injection in left EAC, herpes zoster IgG positive, spontaneous right beating in electronystagmograpy, 54% left canal paresis in Caloric test , decreasing left side Tc in velocity step rotatory test, decresed gain, deviation to left in symmetry and phase lead in sinusoidal harmonic acceleration test, normal range hearing in pure tone audiogram, microangiopathy on cortex in brain MRI and negative PCR. CONCLUSION:This case supports viral infection etiology rather than ischemia in vestibular neuritis. But more studies to find the etiology of vestibular neuronitis are required.
Acceleration
;
Brain
;
Caloric Tests
;
Ear, Inner
;
Female
;
Hearing
;
Herpes Zoster
;
Herpes Zoster Oticus
;
Humans
;
Immunoglobulin G
;
Ischemia
;
Magnetic Resonance Imaging
;
Paresis
;
Physical Examination
;
Polymerase Chain Reaction
;
Reference Values
;
Serologic Tests
;
Vertigo
;
Vestibular Nerve
;
Vestibular Neuronitis
5.CT findings of pleural lesions: differential diagnosis between malignant and benign diseases.
Seung Yon BAEK ; Tae Hwan LIM ; Woo Sun KIM ; Kwang Gil PARK
Journal of the Korean Radiological Society 1991;27(3):351-357
No abstract available.
Diagnosis, Differential*
6.Immunologic and morphologic study of experimental IgA nephropathy in ddY mice after administration of cyclosporin A(CsA).
Woo Young CHUNG ; Soon Yong LEE ; In Sook LIM ; Sun Kyung LEE
Korean Journal of Nephrology 1992;11(2):101-114
No abstract available.
Animals
;
Cyclosporine*
;
Glomerulonephritis, IGA*
;
Immunoglobulin A*
;
Mice*
7.The Effects of Steroid and Lidocaine on Inflammatory Responses in the Rabbit Lung of Induced Endotoxemia.
Yong Gul LIM ; Yoo Jin KANG ; Dae Woo KIM ; Won Sun CHO
Korean Journal of Anesthesiology 1998;35(5):846-851
Background: It has been reported that steroid and lidocaine reduce inflammatory responses to endotoxin. The aim of this study is to compare the effects of lidocaine with those of steroid on inflammatory responses to Escherichia coli endotoxemia in the rabbit lung. Methods: Twenty four animals were randomly assigned to one of four groups. Group I (endotoxemic group; control, n=6): They were received E. coli endotoxin (500 microg/kg) intravenously through the ear vein and followed by saline infusion at 20 ml/kg/hr for 4 hours. Group II (steroid treated group, n=6): They were treated with steroid (30 mg/kg) intravenously just after endotoxin injection and then saline was given at 20 ml/kg/hr for 4 hours. Group III (lidocaine treated group, n=6): They were received same as the endotoxemic group and lidocaine (3 mg/kg IV bolus, then continuous infusion at the rate of 2 mg/kg/hr) was administered with saline at 20 ml/kg/hr for 4 hours. Group IV (steroid & lidocaine treated group, n=6): They were received same as the endotoxemic group and after endotoxin infusion, steroid (30 mg/kg) and lidocaine (3 mg/kg IV bolus, then continuous infusion at the rate of 2 mg/kg/hr) was administered intravenously with saline. Numbers of neutrophils and monocytes in the samples of peripheral blood and lung tissue were counted and compared to evaluate the anti-inflammatory effect of drugs. Results: The group II, III, and IV showed significant attenuation of inflammatory responses compared to group I in the rabbit lung(P<0.05). But there were no significant differences among group II, III, and IV. There was no additive effect between steroid and lidocaine. Conclusion: These results suggest that lidocaine could be used as an alternative drug to steroid for its anti-inflammatory effects and cost-effectiveness.
Animals
;
Ear
;
Endotoxemia*
;
Escherichia coli
;
Lidocaine*
;
Lung*
;
Monocytes
;
Neutrophils
;
Veins
8.Benign compression fractures of the spine: signal patterns.
Kyung Nam RYU ; Woo Suk CHOI ; Sun Wha LEE ; Jae Hoon LIM
Journal of the Korean Radiological Society 1992;28(3):429-434
Fifteen patients with 38 compression fractures of the spine underwent magnetic resonance(MR) imaging. We retrospectively evaluated MR images in these benign compression fractures. MR images showed four patterns in T1-weighted images. MR imaging patterns were normal signal(21), band like low signal(8), low signal with preservation of peripheral portion of the body(8), and diffuse low signal through the vertebral body(1). The low signal portions were changed to high signal intensities in T2-weighted images. In 7 of 15 patients(11 compression fractures). There was a history of trauma, and the remaining 8 patients(27 compression fractures) had no history of trauma. Benign compression fractures of trauma, remained 8 patients(27 compression fractures) were non-traumatic. Benign compression fractures of the spine reveal variable signal intensities in MR imagings. These patterns of benign compression fractures may be useful in interpretation of MR imagings of the spine.
Fractures, Compression*
;
Humans
;
Magnetic Resonance Imaging
;
Retrospective Studies
;
Spine*
9.A clinical study on the idiopathic respiratory distress syndrome of the newborn: comparison of radiographic and clinical findings.
Byoung Yul LIM ; Chong Woo BAE ; Yong Mook CHOI ; Chang Il AHN ; Sun Wha LEE
Journal of the Korean Pediatric Society 1991;34(1):16-23
No abstract available.
Humans
;
Infant, Newborn*
10.A Case of Congenital Dislocation of the Knee.
Seung Geun LIM ; Woo Jin KWON ; Sun Ki LEE ; Jae Ho HYUN ; Jin PARK ; Chun Hee WON ; Young Woo KIM
Korean Journal of Perinatology 1998;9(2):175-179
Congenital dislocation of the knee is very rare skeletal deformity and was firstly described by Chatelaine in 1822. Since then there have been a few number of case reports and some series discussing the etiology and treatment. The tibia is displaced anteriorly in relation to the femur. It is generally subdassified as simple hyperextention, subluxation, and dislocation depending on the degree of the joint displacement and the severity of disease. Early gentle manipulation and serial splintage or plaster cast are recommended for treatment and were successful in the majority except the case of quadriceps contrarture or late correction. The authors experienced one case of congenital dislocation of the right knee and brief review of the related literatures was made.
Casts, Surgical
;
Congenital Abnormalities
;
Dislocations*
;
Femur
;
Joints
;
Knee*
;
Tibia