There are substantial evidences suggesting that DNA content of tumors may provide the prognostic information with independent significances. In this study, the results of DNA ploidy analyzed by image cytometry on touch imprint and by flow cytometry on fresh cell suspension of 78 solid tumors were compared. For 68 cases, there was an excellent correlation between two methods. For 6 cases, an aneuploid population was found by image, but not by flow cytometry one case had an aneuploid peak by flow cytometry. Two methods may use in a complementary fashion m identify as many aneuploid cell population as possible.

This study was carried out to demonstrate the mode of ADR-induced cell death(apoptosis) on the light and electron microscopic features, to measure the apoptotic index dependent on various doses of ADR, to investigate the possible mechanism of apoptosis induced by ADR, and to evaluate ISNT method for the detection of DNA strand break. HeLa cells were treated with various doses of ADR 0.1~100.0 microgram/ml and observed under the light and transmission electron microscopes at 6 hours, 1 day and 3 days after ADR treatment. In addition, DNA strand breaks induced by ADR were detected in HeLa cells using the in situ nick translation(ISNT) method. The results were as follows: 1) The cell viability of HeLa cells decreased and the apoptotic index increased following exposure to ADR in a dose-dependent manner, resulting in about 44% of apoptotic index at 100.0 microgram/ml of ADR treatment. 2) Light microscopically, HeLa cells treated with ADR showed shrinkage or condensation of nucleus and cytoplasm. There were various unclear changes showing irregular, large, delineated masses of condensed chromatin abutting on the nuclear envelopes. Later stage of apoptosis revealed contracted and condensed cytoplasm with irregular cell membrane. Electron microscopically, margination of condensed chromatin, dilatation of endoplasmic reticulum under the plasma membrane, aggregation of cytoplasmic organelles with morphologically intact mitochondria, and irregular cell surface with blebbing were observed. 3) ISNT using biotinylated dUTP exhibited strong positive nuclear staining in HeLa cells treated with ADR. There was a marked response at 10.0~20.0 microgram/ml of ADR treatment. It is concluded from the above results that the death of HeLa cells induced by ADR was apoptotic in type based on light and electron microscopic appearance. The apoptotic index correlated with the increasing dose of ADR. ISNT with biotinylated dUTP led to visible evidence of DNA strand breaks following ADR treatment of HeLa cells. ISNT can be used for detection of DNA degradation, caused by activation of endogenous endonuclease, which is an early and specific characteristic of apoptosis.

The contamination period of the sterilized pack is influenced by the storage environment such as air contamination, temperature, humidity and the quality of the wrapping material. This study was conducted to provide basic data, for the expiration date of the EO gas sterilized pack (which was wrapped with polypropylene / polyester one side and paper the other side) by investigating the storage environment and monthly contamination period. The duration of study was 12 months, from Dec. 1, 1997 to Nov. 30, 1998. A total of 144 EO gas sterilized packs were kept in six storage cabinets of storage area after selecting four Operating Room, the Central Supply Department and General Surgery Clinic of one University Hospital in Taegu city. The samples were cultured in fluid thioglycolate media 30m1 in 37degrees C incubator for 48 hours. Air microbial bioburden in the six storage area and storage cabinet was monitored by exposing blood agar plate for fit teen minutes and culture were observed at 37t incubator for 2 thours. Temperature and Humidity were investigated four times(8 am, 2, 5, & 10 pm) a day and the mean values calculated. The results of this study are as follows 1. There was no contaminations in EO gas sterilized packs for 12 months observation. 2. The mean air contamination rate of storage area and cabinet in Operating Room was 4.9 CFU and 2.4 CPU during observation period. The mean air contamination rate of storage area and cabinet was 4.2 CPU and 2.2 CPU each during observation period. The rate of air contamination in storage room was at least twice than that of storage in Operating Room. The period that showed highest air contamination was in Feb. 98 as 9.2 CPU and the lowest in Mar. 98 as 1.1 CPU. 3. The predominant microorganisms were gram post five staphylococci, gram negative staphylococci in all storage environment, gram positive bacilli, gram negative bacilli in C cabinet of Operating Room and storage area in General Surgery Clinic, Fungi in storage area of Operating Room. 4. The mean temperature was 24.9+/-0.6degrees C(mean standard deviation) in storage area and 24.8+/-0.5degrees C in storage cabinet of Operating Room. The temperature was not differentiated between storage area and cabinet of Operating Room. The mean humidity was 63.6 7.3% (mean standard deviation) in storage area, 663+/-7.9% in cabinet of Operating Room. The humidity was observed high rate in storage area than cabinet of the six area environment. There was no growth of microorganism in sterilized packs during the observation. So we concluded that the contamination period of EQ gas sterilized packs was over 12 months. The high rate of air contamination in storage area might increase the degree of contamination of sterilized packs and the infection rate of operative wound. So the methods which could reduce the air contamination should be developed.
Adolescent ; Agar ; Daegu ; Ethylene Oxide* ; Fungi ; Humans ; Humidity ; Incubators ; Operating Rooms* ; Polyesters ; Polypropylenes ; Wounds and Injuries

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The beneficial effects of sunlight and ultraviolet light for the treatment, of psoriasis vulgaris has long been recognized. Parrish et al were the first to introduce a controlled light source, which allowed the delivery of a known quantity of light energy to the patients skin after the patient had ingested a photosensitizer, 8-methoxypsoralen (methoxsalen) two hours previous to UVA exposure. This successful treatment has become known as PUVA (psoralen and UVA). PUVA photochernotherapy denotes a systemic approach that is based on the interaction of light and a photoactive drug. Parrish et al have described successful treatment of psoriasis with PUVA and other investigators alI over the world have confirmed their beneficial results. The rationale of photochemotherapy in psoriasis is the inhibition of the increased DNA synthesis within the psoriatic lesions by the interaction of psoralen molecule and light energy in the UVA range(320-400nm). The present study was undertaken to evaluate the therapeutic effectiveness of PUVA photochernotherapy combining orally administered methoxsalen and UAA exposure using Dermatron UVA phototherapy system in patients with wi5espread psoriasis. A total of 15 patients with psoriasis over more than 30% of their bodies were selected at the department of dermatology, National Medical Center through March 1978 to September 1978. Oral administration of methoxsalen, 20 to 50 mg doses according to body weight, was followed by exposure to a high-intensity longwave ultraviolet light emitting a continuous spectrum between 320 and 400nm (peak emission, 350 - 365nm). Initial UVA exposure times were based on each patients minimal phototoxicity dose (MPD) and exposure times were increased at each treatment by 1 - 2 minutes to maintain minimal erythema. Frequency of treatment was two to three times weekly. Once the psoriatic lesions had, cleared completely the patient was placed on a maintenance schedule. If psoriasis recurred the frequency of treatment was increased. The results were as follows: l. 5 patients were dropped out due to unknown reasons. 2. 7 cases showed clearing of over 95, of the lesions (Response 5'). Mean number of PU VA therapy was 16. 7. During 1 4 months follow-up period, 4 cases remained free of psoriasis but 3 cases couldn't be followed. 3. Immediate side effects of PUVA were temporary and generally mild. No significant changes in laboratory screenings were noted. Side effects of therapy included transient nausea(33.3%), dizziness(26.7%), erythema.(66.7%), pruritus (60%), increased pigrnentation(100 %). 4. Comparison of pre-and post-PUVA therapy in biopsy specimens by light microscopy revealed histologic improvement evidenced by diminished acanthosis and parakeratosis, and regeneration of granular layers in all of 8 cases. Glycogen accumulation in the affected epiderrnis was significantly decreased or absent after PUVA therapy in all cases.
Administration, Oral ; Appointments and Schedules ; Biopsy ; Body Weight ; Dermatitis, Phototoxic ; Dermatology ; DNA ; Erythema ; Ficusin ; Follow-Up Studies ; Glycogen ; Humans ; Mass Screening ; Methoxsalen ; Microscopy ; Parakeratosis ; Photochemotherapy* ; Phototherapy ; Pruritus ; Psoriasis* ; PUVA Therapy ; Regeneration ; Research Personnel ; Skin ; Sunlight ; Ultraviolet Rays

PURPOSE: This study was to investigate effects of the dementia family education program on dementia recognition, burden, and depression in caregivers of elders with dementia. METHODS: Aquasi-experimental research with a nonequivalent control group non-synchronized pre and post design was used with 52 caregivers of elders with dementia living in D city. Data were collected from August 12 to December 12, 2013. The dementia family education program was provided to the experimental group once a week over 8 weeks, for 90 minutes per session. Data were analyzed using χ² test, Fisher exact probability test, t-test. RESULTS: Experimental group participants who took part in the dementia family education program had higher dementia recognition scores than the control group that did not participate (t=3.79, p<.001). Experimental group participants who took part in the dementia family education program had lower burden and depression scores than the control group that did not participate (t=-2.90, p=.006) (t=-2.22, p=.034). CONCLUSION: The findings indicate that the dementia family education program enhances dementia recognition by caregivers of elders with dementia and lowers burden and depression in these caregivers. Use of the dementia family education program is recommended as an effective nursing intervention for caregivers of elders with dementia.
Caregivers* ; Dementia* ; Depression* ; Education* ; Humans ; Nursing

The technique of radial artery cannulation and its complications are well documented, but serious complications are rare. This is a report of one case of amputation of wrist due to finger necrosis developed from the radial artery cannulation in patient who had craniectomy surgery. This 52-year-old 79 kg male underwent subdural hematoma removal surgery. Right radial artery cannulation was carried out percutaneously using 22 gauge Teflon extracath needle after modified Allen's test appeared to be positive. It was intermittently flushed by heparinized solution. His arterial blood pressure was maintained 100/70-110/80 mmHg and 5 units of banked whole blood and 1 unit of fresh frozen plasma were transfused during 8-hours operation. Cannula was removed on the 9th hour after operation because that was obstructed. On the 12th hour after removal of cannula, his right hand noted to be cool and cyanotic. So, warm towel and hot bag applied continuously on the right hand and the right stellate ganglion block was carried out every day for 4 times. However, on the 10th day after removal of cannula, necrotic change of all fingers of the right hand became worse and skin of fingers were shrunken. Therefore, disarticulation of the right wrist carried out on the 71th day of his hospitalization.
Amputation* ; Arterial Pressure ; Catheterization* ; Catheters ; Disarticulation ; Extremities* ; Fingers ; Hand ; Hematoma, Subdural ; Heparin ; Hospitalization ; Humans ; Male ; Middle Aged ; Necrosis ; Needles ; Plasma ; Polytetrafluoroethylene ; Radial Artery* ; Skin ; Stellate Ganglion ; Wrist

BACKGROUND: Cardiac hypertrophy is the compensatory response of the myocadium to increased workload. Compensatory mechanisms come into play when the hypertrophied heart can no longer accommodate the increased demand or persistent stimuli. Although it has been reported that the molecular changes in hypertrophied hearts that initially mediate enhanced function may contribute to the development of heart failure, the structural/biochemical/molecular basis for myocardial contractile failure is still obscure. This study was aimed to clarify the structural basis for relation between hypertrophy and failure. METHOD: Nine pairs of rabbits were sacrificed at 8,12,24,48 hours and 1,2,4,6,8 weeks after experiment aortic constriction. There hearts were studied with routine histopathology. Each heart was weighed and compared with total body weight. Multiple sections were embedded in paraffin, sectioned at 5um, and stained with hematoxylin and cosin and Masson's trichrome and analysed. RESULTS: The heart weight to body weight ratio(g/Kg) increased progressively with time after aortic banding. Banding of the aorta in the rabbit resulted in multifocal areas of myofiber degeneration, necrosis and fibrosis through the wall of the left ventricle, in the papillary muscles of the left ventricle and in the left ventricular portions of the interventricular septum in rabbit of 6 and 8 weeks after aortic banding. By 4 weeks after banding, the foci of necrosis were not observed. CONCLUSION: There findings suggest that the increased necrosis, fibrosis in animals with cardiac hypertrophy induced by banding the aorta may play a role in progression to heart failure.
Animals ; Aorta ; Body Weight ; Cardiomegaly ; Constriction ; Fibrosis ; Heart Failure ; Heart Ventricles ; Heart* ; Hematoxylin ; Hypertrophy ; Necrosis* ; Papillary Muscles ; Paraffin ; Rabbits

No abstract available.
Blood Transfusion* ; Female ; Humans

OBJECTIVE: The purpose of this study were to: (1) describe the individual characteristics, perceived health status, body image, and health promoting behaviors of staff nurses working in a metropolitan hospital and (2) determine the relationship of perceived health status, body image, and health promoting behaviors. METHODS: Data were collected from 311 staff nurses working at one of university affiliated hospitals using a self-administered questionnaire for perceived health status(SF 36 V2), body image(BAQ), and health promoting behaviors (HPLP-II) and using the report of year 2002 employee physical check-up results for health related characteristics. RESULTS: Most of nurses were within the normal range of BMI, total cholesterol, and liver enzymes but 42.2% had systolic BP above 120mmHg and 37.3% hemoglobin below 12g/dL. Although 96.4% of BMI score indicated 'underweight' or 'normal', 'feeling fat' showed the highest. Among health promoting behaviors the most frequently reported one was spiritual growth and the least one was engagement in physical activity. In the correlational analysis, health promoting behaviors had the positive relationships with perceived health status, vitality, mental health, attractiveness, strength and fitness (p=.000 - .004). CONCLUSION: These findings provide information that is relevant in designing interventions to enhance health promoting behaviors among nurses working in a hospital.
Body Image* ; Cholesterol ; Hospitals, Urban ; Liver ; Mental Health ; Motor Activity ; Reference Values ; Surveys and Questionnaires