1.Phospholipase D2 promotes degradation of hypoxia-inducible factor-1alpha independent of lipase activity.
Mi Hee PARK ; Sun Sik BAE ; Kang Yell CHOI ; Do Sik MIN
Experimental & Molecular Medicine 2015;47(11):e196-
Hypoxia-inducible factor-1alpha (HIF-1alpha) is a key transcriptional mediator that coordinates the expression of various genes involved in tumorigenesis in response to changes in oxygen tension. The stability of HIF-1alpha protein is determined by oxygen-dependent prolyl hydroxylation, which is required for binding of the von Hippel-Lindau protein (VHL), the recognition component of an E3 ubiquitin ligase that targets HIF-1alpha for ubiquitination and degradation. Here, we demonstrate that PLD2 protein itself interacts with HIF-1alpha, prolyl hydroxylase (PHD) and VHL to promote degradation of HIF-1alpha via the proteasomal pathway independent of lipase activity. PLD2 increases PHD2-mediated hydroxylation of HIF-1alpha by increasing the interaction of HIF-1alpha with PHD2. Moreover, PLD2 promotes VHL-dependent HIF-1alpha degradation by accelerating the association between VHL and HIF-1alpha. The interaction of the pleckstrin homology domain of PLD2 with HIF-1alpha also promoted degradation of HIF-1alpha and decreased expression of its target genes. These results indicate that PLD2 negatively regulates the stability of HIF-1alpha through the dynamic assembly of HIF-1alpha, PHD2 and VHL.
Cell Line
;
HEK293 Cells
;
Humans
;
Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
;
Phospholipase D/*metabolism
;
Prolyl Hydroxylases/metabolism
;
Proteasome Endopeptidase Complex/*metabolism
;
*Protein Interaction Maps
;
Proteolysis
;
Ubiquitin-Protein Ligases/metabolism
;
Von Hippel-Lindau Tumor Suppressor Protein/metabolism
2.A Case of Cervical Pregnancy Treated with Intramuscular Methotrexate Injection.
Tae Yeop LEE ; Du Sik KONG ; Doo Jin BAE ; Sun Do HONG ; Yun Jung PARK ; Young Wook SUH
Korean Journal of Obstetrics and Gynecology 2000;43(5):897-900
Cervical pregnancy is a rare form of ectopic gestation in which the blastocyst implants in the cervical mucosa below the histologic cervical os. Because of the serious vaginal bleeding, hysterectomy was usually done in the management of cervical pregnancy. Howerver, conservative treatment is desirable for women who want to be pregnancy in the future. Methotrexate has been utilized recently for conservative management of cervical pregnancy. We report a case of cervical pregnancy which was treated succesfully with intramuscular methotrexate injection.
Blastocyst
;
Female
;
Humans
;
Hysterectomy
;
Methotrexate*
;
Mucous Membrane
;
Pregnancy*
;
Uterine Hemorrhage
3.Prevalence and Characteristics of Chemotherapy-related Cognitive Impairment in Patients with Breast Cancer.
Jin Hee PARK ; Sun Hyoung BAE ; Yong Sik JUNG ; Young Mi JUNG
Journal of Korean Academy of Nursing 2015;45(1):118-128
PURPOSE: Evidence suggests that some patients with breast cancer experience cognitive difficulties following chemotherapy. This longitudinal study was done to examine the prevalence of cognitive impairment and trajectory of cognitive function over time in women with breast cancer, who received adjuvant chemotherapy. METHODS: Participants were 137 patients with breast cancer. They completed neuropsychological tests and the Functional Assessment of Cancer Therapy-Cognitive Function before adjuvant therapy (pretest), toward the end of adjuvant therapy (posttest), and 6 months after the completion of adjuvant therapy (follow-up test). Of the patients, 91 were treated with adjuvant chemotherapy and 46 patients who did not receive chemotherapy made up the comparison group. A reliable-change index and repeated-measure ANOVA were used for statistical analyses. RESULTS: At the posttest point, over 30% of patients showed complex cognitive impairment and reported greater difficulty in subjective cognitive function. At the follow-up test point, 22.0% of patients exhibited complex cognitive impairment and 30.8% of patients complained of subjective cognitive impairment. Repeated-measure ANOVA showed significant decreases after receiving chemotherapy followed by small improvements 6 months after the completion of chemotherapy in cognitive domains of change for attention and concentration, memory, executive function, and subjective cognitive function. CONCLUSION: These results suggest that chemotherapy in patients with breast cancer may be associated with objective and subjective cognitive impairments. Further studies are needed to explore the potential risk factors and predictor of chemotherapy-related cognitive changes. Also nursing interventions for prevention and intervention of cognitive impairments should be developed and tested.
Adult
;
Analysis of Variance
;
Antineoplastic Combined Chemotherapy Protocols/adverse effects/*therapeutic use
;
Attention/drug effects
;
Breast Neoplasms/*drug therapy
;
Chemotherapy, Adjuvant
;
Cognitive Dysfunction/epidemiology/*etiology
;
Female
;
Humans
;
Longitudinal Studies
;
Memory/drug effects
;
Middle Aged
4.A case of concomittantly occurred bilateral adrenal medullary hyperplasia and a ganglioneuroma near the left adrenal gland.
Hoon Sik KIM ; Jun Young PARK ; Hak Sun KIM ; Kyo Il SUH ; Myung Hi YOO ; Guk Bae KIM ; So Young JIN ; Dong Hwa LEE
Journal of Korean Society of Endocrinology 1991;6(3):259-265
No abstract available.
Adrenal Glands*
;
Ganglioneuroma*
;
Hyperplasia*
5.Laparoscopic Cholecystectomy in Patients with a History of Upper Abdominal Surgery.
Young Sik BAE ; Sang Mok LEE ; Joo Hyun KIM ; Bum Soo KIM ; Sun Hyung JOO
Journal of the Korean Society of Endoscopic & Laparoscopic Surgeons 2009;12(2):108-112
PURPOSE: Laparoscopic cholecystectomy is currently the treatment of choice for gallbladder disease. Previous abdominal surgery was considered as a relative contraindication for laparoscopic cholecystectomy due to the presence of intraabdominal adhesion and the difficulty in visualization. Several recent studies have suggested that previous abdominal surgery is no longer a contraindication for laparoscopic surgery by virtue of the accumulation of surgeons' experience and the evolution of laparoscopic instruments. In this study, we evaluated the impact of previous upper abdominal surgery on laparoscopic cholecystectomy. METHODS: The data of 706 consecutive patients who underwent laparoscopic cholecystectomy from September 2004 to December 2007 was retrospectively analyzed. Thirty-three patients had undergone pervious upper abdominal surgery and 673 patients had not. We compared the operative time, the type of trocar that was used, the postoperative complications, the frequency of open conversion and the length of the postoperative hospital stay between the two groups RESULTS: The operative time was longer (141.8+/-88.7 min vs. 74.1+/-37.4 min, p<0.001) and larger trocars were used more often (p<0.001) in the previous upper abdominal surgery group. There was no significant difference in the open conversion rate, the major postoperative complication rate and the length of the postoperative hospital stay. CONCLUSION: Laparoscopic cholecystectomy might be an optimal treatment for patients with a history of previous upper abdominal surgery. Previous upper abdominal surgery is not a contraindication for laparoscopic cholecystectomy when it is performed by experienced laparoscopic surgeons.
Cholecystectomy, Laparoscopic
;
Gallbladder Diseases
;
Humans
;
Laparoscopy
;
Length of Stay
;
Operative Time
;
Postoperative Complications
;
Retrospective Studies
;
Surgical Instruments
;
Virtues
6.A New Gene Regulated by Electroconvulsive Shock in Rat Brains.
Sun Ju CHUNG ; Jeong Eun PARK ; Ung Gu KANG ; Young Jin KOO ; Joo Bae PARK ; Yong Sik KIM
Journal of Korean Neuropsychiatric Association 2000;39(5):928-935
OBJECTIVES: This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions. METHODS: ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA differential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus. RESULTS: By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain. CONCLUSION: In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain.
Adult
;
Animals
;
Blotting, Northern
;
Brain*
;
Clone Cells
;
Cloning, Organism
;
Electroshock*
;
Hippocampus
;
Humans
;
Male
;
Mass Screening
;
Rats*
;
Rats, Sprague-Dawley
;
RNA, Messenger
7.Phytosphingosine-1-phosphate stimulates chemotactic migration of L2071 mouse fibroblasts via pertussis toxin-sensitive G-proteins.
Mi Kyoung KIM ; Kyoung Sun PARK ; Hyuck LEE ; Young Dae KIM ; Jeanho YUN ; Yoe Sik BAE
Experimental & Molecular Medicine 2007;39(2):185-194
Phytosphingosine-1-phosphate (PhS1P) was found to stimulate an intracellular calcium increase via phospholipase C but not pertussis toxin (PTX)- sensitive G-proteins in L2071 mouse fibroblasts. PhS1P also activated ERK and p38 kinase, and these activations by PhS1P were inhibited by PTX. Moreover, PhS1P stimulated the chemotactic migration of L2071 cells via PTX-sensitive Gi protein(s). In addition, the PhS1P-induced chemotactic migration of L2071 cells was also dramatically inhibited by LY294002 and SB203580 (inhibitors of phosphoinositide 3-kinase and p38 kinase, respectively). L2071 cells are known to express four S1P receptors, i.e., S1P1, S1P2, S1P3, and S1P4, and pretreatment with an S1P1 and S1P3 antagonist (VPC 23019) did not affect on PhS1P-induced chemotaxis. This study demonstrates that PhS1P stimulates at least two different signaling cascades, one is a PTX-insensitive but phospholipase C dependent intracellular calcium increase, and the other is a PTX-sensitive chemotactic migration mediated by phosphoinositide 3-kinase and p38 kinase.
1-Phosphatidylinositol 3-Kinase/metabolism
;
Animals
;
Calcium Signaling/drug effects
;
Chemotaxis/*drug effects
;
Estrenes/pharmacology
;
Extracellular Signal-Regulated MAP Kinases/metabolism
;
Fibroblasts/*cytology/*drug effects
;
GTP-Binding Proteins/*metabolism
;
Gene Expression Regulation/drug effects
;
Humans
;
Mice
;
Pertussis Toxin/*pharmacology
;
Phosphorylation/drug effects
;
Pyrrolidinones/pharmacology
;
RNA, Messenger/genetics/metabolism
;
Receptors, Lysosphingolipid/genetics/metabolism
;
Sphingosine/*analogs & derivatives/pharmacology
;
p38 Mitogen-Activated Protein Kinases/metabolism
8.Intrinsic Endometriosis of Ureter: A Case Report.
Myung Sun HONG ; Ho Chul KIM ; Ku Sup YUN ; Chul Soon CHOI ; Sang Hoon BAE ; Sung Yong KIM ; Hyung Sik SHIN
Journal of the Korean Radiological Society 1995;33(1):109-112
Endometriosis is a rare cause of an ureteral obstruction. We report a case of intrinsic ureteral endometriosis resulting in severe hydroureteronephrosis. The diagnosis of ureteral endometriosis may be considered in women with flank pain and ureteric obstruction within true pelvis.
Diagnosis
;
Endometriosis*
;
Female
;
Flank Pain
;
Humans
;
Lesser Pelvis
;
Ureter*
;
Ureteral Obstruction
9.Factors Influencing Elevated Distress Scores at the End of Primary Treatment of Breast Cancer.
Jin Hee PARK ; Sun Hyoung BAE ; Mison CHUN ; Yong Sik JUNG ; Young Mi JUNG
Asian Oncology Nursing 2015;15(3):132-139
PURPOSE: The purpose of this study was to determine the prevalence of distress and to investigate the related problems in breast cancer patients at the end of primary treatment. METHODS: A sample of 118 participants was recruited among outpatients who had successfully completed primary treatment of breast cancer. Data were collected between July, 2013 and October, 2014 using the Distress Thermometer (DT) scale and problem lists. Descriptive statistics and logistic regression analysis were used to analyze data. RESULTS: Among the 118 patients that participated in this study, 34 patients (28.8%) suffered from elevated distress using the previously validated cut point > or =4 for the DT. Problems most frequently encountered were fatigue (68.8%), worry (59.3%), appearance (51.7%) and memory/concentration (48.3%). Multivariate logistic regression analysis revealed that depression (OR=9.55), worry (OR=8.34), fatigue (OR=8.19) and age (OR=1.14) were independent predictors for elevated distress scores. CONCLUSION: Utilizing the DT, onethird of breast cancer patients screened met criteria for distress at the end of the primary treatment. Breast cancer patients with depression, worry, fatigue and older age should be targeted for distress screening and management.
Breast Neoplasms*
;
Breast*
;
Depression
;
Fatigue
;
Humans
;
Logistic Models
;
Mass Screening
;
Outpatients
;
Prevalence
;
Survivors
;
Thermometers
10.Three cases of pacemaker-mediated tachycardia.
Kae Won LEE ; Chung Whee CHOUE ; Woo Sik KIM ; Heung Sun KANG ; Kwon Sam KIM ; Jung Sang SONG ; Jong Hoa BAE
Korean Circulation Journal 2000;30(3):334-338
Pacemaker-mediated tachycardia (PMT) is a well-known complication of dual-chamber pacemaker with atrioventricular sensing and pacing (DDD) or atrial synchronous, ventricular demand pacemaker (VDD). PMT usually starts with sensing a retrograde P waves linked to ventricular extrasystole with retrograde ventriculoatrial conduction and forms reentrant or circus movement tachycardia with the pacemaker itself as an antegrade limb and the conducting tissue of the heart as a retrograde limb. Recently, a number of pacemaker manufactures have incorporated in their devices a variety of relatively complex algorithms to prevent PMT. Despite these measures, PMT may still occur because of inappropriate programming or unpredictable variations of ventri-culoatrial conduction. We report two cases of PMT in 78 year-old man and 60 year-old man who received DDD type pacemakers due to sick sinus syndrome, and a case of PMT in 69 year-old man who had suffered complete heart block and received a VDD type pacemaker. In these cases, we investigate symptoms, mechanism of PMT, programed parameters of pacemaker and treatments of PMT.
Aged
;
Dichlorodiphenyldichloroethane
;
Extremities
;
Heart
;
Heart Block
;
Humans
;
Middle Aged
;
Sick Sinus Syndrome
;
Tachycardia*
;
Ventricular Premature Complexes