Interferons(IFNs) exhibit an antiproliferative effect on many normal and transformed cells and have in vivo antitumor activity in a variety of cancers. Recent clinical studies have suggested major activity with IFNs, especially in advanced squamous cell carcinoma of the skin and cervix. With the objective of exploring how the IFNs might work in squamous carcinoma cell line, we studied the effect of IFN-a on cervical cancer cell lines. The effect of IFNs on apoptosis and cell cycle of cervical cancer cell lines(C33A, CaSki, SiHa, HeLa, ME-180) was analysed by flow cytometry in time dependent manner. Results were as follows: (1) According to cell count of studied cancer cell lines treated with 2,000 IU/ml IFN-a for 7 days exposure, IFN-a had the antiproliferative effect on all five tested cervical cancer cell lines. Also this antiproliferative effect was confirmed by WST-1 assay. (2) The effect of IFN-a on apoptosis of each cultute was analysed by flow cytometry after 3 days and 7 days exposure with 2,000 IU/ml IFN-a, Apoptosis was not induced by IFN-a treatment. (3) The effect of IFN-a on the cell cycle of each culture was analysed by flow cytometry after 3 days exposure with 2,000 IU/ml IFN-a. As compared to control cells, treatment with IFN-a resulted in a higher proportion of cells in S phase with lower portion of cells with G2/M phase. (4) Time course of IFN-a effect on HPV 16 and HPV 18 E6 mRNA levels was evaluated by northern blot analysis. In CaSki cell line, HPV 16 E6 mRNA expression induced by IFN-a was not inhibited. But in HeLa cell line, HPV 18 E6 inRNA expression was inhibited. IFN-a appears to have the antiproliferative effect on all five tested cervical cancer cell lines and the antiproliferative effect of IFN-a seemed to be induced not by apoptosis but by disruption on specific cell cycle. Also regulation of HPV E6 mRNA expression induced by IFN-a is not directly related to the mechanisms of the antiproliferative effect of IFN-a in cancer cell lines.
Apoptosis ; Blotting, Northern ; Carcinoma, Squamous Cell ; Cell Count ; Cell Cycle ; Cell Line* ; Cervix Uteri ; Female ; Flow Cytometry ; HeLa Cells ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans ; RNA, Messenger ; S Phase ; Skin ; Uterine Cervical Neoplasms*

PURPOSE: Acute diarrhea in young children is a major problem in pediatric hospitals worldwide. We evaluated the clinical efficacy of orally administered Lactobacillus acidophilus in the treatment of acute diarrhea in children. METHODS: From September 2002 to July 2003 at National Police Hospital 41 children aged 3 months to 5 years with acute diarrhea were enrolled in this study. The patients were randomized to one of two groups to receive either 0.5 x 10(8) colony forming unit (CFU) of L. acidophilus or matching placebo on admission and every 8 hours during hospitalization. RESULTS: The mean duration of diarrhea in all 41 children was decreased (p=0.001) in the L. acidophilus (40.5 hours) group compared to the placebo (56.6 hours) group. Stool frequency was also reduced (p=0.01) on the 3rd day in the L. acidophilus group. Rotavirus was identified in 58% of the patients. The decrease of duration of diarrhea was more significant in rotavirus-negative patients (p=0.002) compared to the rotavirus-positive patients (p=0.027). CONCLUSION: L. acidophilus shows to be an effective therapeutic agent in acute diarrhea in children. Further studies are needed to confirm the present findings.
Child* ; Diarrhea* ; Hospitalization ; Hospitals, Pediatric ; Humans ; Lactobacillus acidophilus* ; Lactobacillus* ; Police ; Probiotics ; Rotavirus ; Stem Cells

PURPOSE: This study was done to provide data that would contribute to effective task performance in clinical nursing and development of nursing professional by understanding job stress, job satisfaction and organizational commitment according to Life Organization (LIFO) behavior types of novice nurses. METHODS: This study was a cross-sectional survey and the participants were 313 novice nurses who worked in one university hospital located in the city of Gwangju. Data were collected through the structured questionnaires that included general characteristics, job stress, job satisfaction, and organizational commitment scales. Collected data were analyzed using descriptive statistics, and ANOVA with SPSS/WIN 19.0. RESULTS: Regarding job stress according to LIFO behavior types of novice nurses, there were statistically significant differences between the favorable situation and the unfavorable situation. There were also statistically significant differences regarding the organizational commitment according to LIFO behavior types. CONCLUSION: The results indicate a difference in behavior patterns of novice nurses that suggest a need for education in order to reduce stress from the job and enhance organizational commitment by developing each nurse's preferred pattern and compensating for unfavorable patterns, as well. There also needs to be nursing resources that take into consideration behavior patterns of novice nurses.
Cross-Sectional Studies ; Education ; Gwangju ; Job Satisfaction* ; Nursing ; Surveys and Questionnaires ; Task Performance and Analysis ; Weights and Measures

PURPOSE: This study was done to investigate compliance with health behaviors among patients who are hypertensive. METHOD: The participants in this study were 121 outpatients with hypertension. Compliance with health behaviors was measured using a questionnaire developed by the researchers. Data were analyzed using t-test, ANOVA, Duncan's multiple range test and chi-square test. RESULTS: According to the general characteristics of the participants, compliance with medication and diet was higher among women than men, among high school graduates than middle school graduates or those with lower academic qualifications, and among those who were unemployed compared to those who were employed. Compliance with alcohol abstinence or not smoking was higher among women than men, and among those who were unemployed compared to those who were employed. According to the risk factors of hypertension, compliance with health behaviors was significantly higher in those who had associated diseases. The participants in the group with a hypertension morbidity period longer than 10 years did exercise more than 3 times a week and regularly weighed themselves once or more a week. The item showing highest compliance concerning medication and diet was "Take prescribed drugs regularly", while the one showing lowest was "Take drugs with knowledge about their side effects".
Alcohol Abstinence ; Compliance* ; Diet ; Female ; Health Behavior* ; Humans ; Hypertension ; Male ; Outpatients ; Surveys and Questionnaires ; Risk Factors ; Smoke ; Smoking

PURPOSE: This study purposes to examine the knowledge level and compliance of health behavior in patients with myocardial infarction to develope a cardiac rehabilitation program. METHOD: The subjects consisted of 72 patients with myocardial infarction, hospitalized at three university-affiliated hospitals. The data were collected by interviewing their subjects using a questionnaire and reviewing the medical records from September 15, 1999 to July 31, 2000. Data were analyzed using the SAS program for Windows version 6.12. RESULTS: 1. The average knowledge score of the patients was 19.7, and the average compliance score was 53.9. 2. Knowledge scores were highest in the items of avoiding overeating and taking medicine at prescribed dosage, and lowest in the item of when to avoid sexual activity. 3. Knowledge level were highest on domains of exercise & daily activities, and risk factors and followed by diet, medication, and nature of disease. 4. Those who had higher education, or were living with a spouse were significantly higher in knowledge score. 5. Compliance score was highest in the item of smoking cessation and lowest in the item of measuring heart rate regularly. 6. Compliance score was highest on domain of smoking cessation and followed by diet, exercise, others, and managing mental stress. 7. Female patients had significantly higher compliance scores of health behavior on domain of diet than male patients. 8. The knowledge score was positively correlated to compliance of health behaviors. CONCLUSION: According to the above findings, it can be concluded that intensive nursing care and education should be provided to the patients who have lower education or are living without a spouse. Also, nursing intervention should be developed to increase compliance of managing mental stress and doing regular exercise.
Compliance* ; Diet ; Education ; Female ; Health Behavior* ; Heart Rate ; Humans ; Hyperphagia ; Male ; Medical Records ; Myocardial Infarction* ; Nursing ; Nursing Care ; Surveys and Questionnaires ; Rehabilitation ; Risk Factors ; Sexual Behavior ; Smoking Cessation ; Spouses

BACKGROUND: Growth retardation (GR) has literally hundreds of causes that have differences in prognoses, complications, and responses to treatments. Especially, growth retarded patients resulting from chromosomal disorders should be genetically counseled. To focus the cytogeneticist's attention on specific chromosomal regions, it is important to understand cytogenetic aberrations associated with GR prior to chromosomal analysis. So, we attempted to figure out the cytogenetic findings of patients with GR and support the effective application of cytogenetic studies, accurate diagnosis and genetic counseling. METHODS: Of 203 cases referred for GR, the positive rate and pattern of chromosomal aberrations were retrospectively analyzed with review of associated clinical features. Cytogenetic studies were performd by high resolution banding technique after peripheral T lymphocyte culture and, if necessary, Ag-NOR stain, C-banding and fluorescence in situ hybridization. RESULTS: Forty two (20.7%) patients had abnormal karyotypes. Thirty one (15.2%) patients had well-recognized chromosomal syndromes including Turner, Cri-du-chat, Edward, 13q- and 18p-/ 18q- syndromes. In addition to those, the sporadic chromosomal aberrations of 11 cases were partial monosomy of 11q23, partial trisomy of 1q32, 4p, 9p, and 14q, and ring chromosome 4 and 18, etc. which were not literally established in view of the correlation with phenotype. CONCLUSIONS: The various results of definite or debating chromosomal disorders associated with GR could be used as the data for diagnosis, management, prognosis, and genetic counseling in growth retarded patients. Furthermore, these may provide the background for prospective study to define the new chromosomal disorders.
Abnormal Karyotype ; Chromosome Aberrations* ; Chromosome Deletion ; Chromosome Disorders ; Cytogenetics* ; Diagnosis ; Fluorescence ; Genetic Counseling ; Humans ; In Situ Hybridization ; Lymphocytes ; Phenotype ; Prognosis ; Retrospective Studies ; Ring Chromosomes ; Trisomy

PURPOSE: The purpose of this study was to examine the level of knowledge and learning needs of patients with a permanent pacemaker implantation. METHODS: The participants were 86 patients with a permanent pacemaker implantation. Data were collected during April and May 2002 using a questionnaire survey on knowledge and level of learning need. The collected data were analyzed using the SAS program. RESULTS: 1. The average knowledge score was 13.21. The knowledge level was significantly higher in participants under 50 years of age and for those with higher levels of education. The items with the highest knowledge score was "permanent pacemaker needs regular check ups" and the lowest, "a bath can be taken 1 month after stitches are removed". 2. The total average score for learning needs was 60.78 and the mean item score was 3.04 as measured on a 4-point Likert scale. The item showing the highest learning need was "electrical fields can affect the pacemaker's function" and the lowest learning need was for "sexual activity after permanent pacemaker implantation". CONCLUSION: According to the above findings, it can be concluded that an intensive nursing education program should be developed for patients with a permanent pacemaker especially for those patients over 50 years of age and those with lower levels of education.
Baths ; Education ; Education, Nursing ; Humans ; Learning* ; Surveys and Questionnaires

No abstract available.
Histiocytosis, Langerhans-Cell* ; Scabies*

No abstract available.
Eczema* ; Hand* ; Rubber*