2.Expression of Epstein-Barr Virus Gene Products, Bcl-2 and p53 Proteins in Nasopharyngeal Carcinomas.
Sun Hee YOON ; Kang Suek SUH ; Chang Hun LEE
Korean Journal of Pathology 1997;31(8):723-734
The authors studied EBV genome expression in 40 conventionally processed samples of nasopharyngeal carcinomas (NPC), using in situ hybridization for EBERs and immunohistochemistry for LMP, Bcl-2 and p53 proteins. The NPCs consisted of 6 keratinizing squamous cell carcinomas (KSCs), 13 nonkeratinizing carcinomas (NKCs) and 21 undifferentiated carcinomas (UCs). The results were summarized as follows: 1) EBERs were expressed in 80.0% of all the NPCs (32/40). As for the subtypes, they were detected in 92.3% of NKCs (12/13), in 90.5% of the UCs (19/21), and in 16.7% of the KSCs (1/6). In positive cases, the nuclei of tumor cells displayed uniformly strong staining. 2) LMP was expressed in 10.0% of all the NPCs (4/40), all of which were UC. The LMP expression in the UCs was not correlated to the expression of EBERs, Bcl-2 and p53 proteins. 3) Bcl-2 protein was detected in 85.0% of all the NPCs (34/40). As for the subtypes, they were detected in 92.3% of the NKCs (12/13), in 90.5% of the UCs (19/21), and in 50.0% of the KSCs (3/6). 4) p53 protein was detected in 75.0% of all the NPCs (30/40). As for the subtypes, they were detected in 81.0% of the UCs (17/21), in 69.2% of the NKCs (9/13), and in 66.7% of the KSCs (4/6). 5) In the NPCs the expression of EBER showed a significantly positive correlation with that of p53 or Bcl-2 protein. The above results indicate that the association of EBV with NPC is chiefly with poorly differentiated and undifferentiated carcinomas. Additionally, carcinomas commonly display widespread, strong immunoreactivity of Bcl-2 and p53 proteins over tumor cells. In conclusion, these observations indicate that the EBV-association in NPC appears to contribute to the overexpression of tumor-related genes during carcinogenesis.
Carcinogenesis
;
Carcinoma
;
Carcinoma, Squamous Cell
;
Genome
;
Herpesvirus 4, Human*
;
Immunohistochemistry
;
In Situ Hybridization
3.The Recovery of Epidermal Barrier after Stratum Corneum Injury and the Changes of Epidermal Lipid Contents in Hand Eczema and Normal Subjects.
Seung Hun LEE ; Kyeong Han YOON ; Mi Sun WHANG
Korean Journal of Dermatology 1995;33(6):1053-1059
BACKGROUND: In eczematous skin it is expected that altered defense mechanisms are employed against an injury, which mechanisms include alterations in the stratum corneum barrier function and lipid contents. OBJECTIVE: To investigaten aspect of pathophysiology of hand eczema by comparing the water holding capacities and transepidermal water loss(TEWL) after injuries on the skin and by comparing the epidermal lipid contents between hand eczema and normal subjects. METHODS: The water holding capacities were checked by corneometer, and the TEWL were checked after epidermal barrie! destruction with acetone or adhesive tapes. The epidermal lipids were extracted with acetone, ther (1: 1 v/v) mixture solvent and analysed by HP- TLC. RESULTS: The water holdingapacities were decreased in eczema of the palm, but the recoveries of TEWL and the lipid content did not differ between hand eczema and normal subjects. CONCLUSION: The water holding capacities were decreased in the eczematous skin. But there were no significant differencies in the recovery of destructed epidermal barrier and the epidermal lipid contents.
Acetone
;
Adhesives
;
Defense Mechanisms
;
Eczema*
;
Hand*
;
Skin
;
Water
4.Ectomycorrhizal Roots Collected from the Bases of the four Edible Basidiocarps Around Mt. Wol Ak.
Sang Sun LEE ; Dong Hun KIM ; Hung Chae CHUNG
Mycobiology 2000;28(1):27-32
The ectomycorrhizal roots were collected from the soils around the bases of basidiocarps of the four edible mushrooms in the stands of Pinus densiflora or Querus acutissima communities (Mt. Wol-Ak in Eastern Chung-Puk): The basidiocarps of Tricholoma matsutake (TM), Sarcodon asparatus (SA), S. imbricatum (SI), and Polyozellus multiplex (PM) are usually collected. The ectomycorrhizal roots of TM, PM and SI were related to the roots of P. densiflora, but the other to the roots of Q. acutissima in Korea. Particularly, the basidiocarps of PM were collected in the mixed stand of both P. densiflora and Q. acutissima. The morphologies of the ectomycorrhizal roots were observed to be the yellowish brown coral (dichromatous) or pyramid types in the roots of the pine, but dark brown un-branched sticks (roots) in the ends of ectomycorrhizal roots of Querus plants. The un-branched roots were covered with the dark mycelia (rhizomorph) around them. Therefore, the ectomyorrhizal roots of PM were observed to have two kinds of types; The single blackish un-branched roots were observed to be attached to the yellowish coral type roots. The bundles of TM mycelia were filled with cortical cells (in the roots of P. densiflora), but the mycelia of the other fungi (Aphylloporales) were massed between the cortical cells of P. densiflora or Q. acutissima. Their anatomical and gross features were considered to be simailar but very important in the ectomycorrhizal roots for these edible mushrooms.
Agaricales
;
Anthozoa
;
Fruiting Bodies, Fungal*
;
Fungi
;
Korea
;
Mycorrhizae
;
Pinus
;
Soil
;
Tricholoma
5.A Case of Cryptococcosis with Cutaneous Manifestation.
Seong Hun LEE ; Sun Young MOON ; Joo Heung LEE ; Seung Chul LEE ; Young KIM
Korean Journal of Dermatology 1995;33(5):935-939
Cryptococcosis is a disease caused by the Cryptococcus neoformans, occcuring most frequently in immunocompromised hosts. Cutaneous involvement is seen in 10-15 % of disseminated cases and its manifestation is variable. A 52 year old man presented with a subcutaneous neck mass and severe headache which had lasted for 2 months and 1 month respectively. Initially cutaneous involvement was monomorphic and localized and a CSF study failed to reveal any organisms. After several weeks of herb medication, however, multiple skin lesions occurred with varied morphology and a CSF study confirmed cryptococcosis by culture. The Urine cortisol was markedly elevated, suggesting an exogenous intake of steroid.
Cryptococcosis*
;
Cryptococcus neoformans
;
Headache
;
Humans
;
Hydrocortisone
;
Immunocompromised Host
;
Middle Aged
;
Neck
;
Skin
6.The effect of hypertension on the progression of renal insufficiency in chronic renal failure patients.
Dae Suk HAN ; Kyu Hun CHOI ; Young Ki KIM ; Dong Hun CHA ; Ho Yung LEE ; Kyo Sun KIM
Korean Journal of Nephrology 1991;10(2):135-144
No abstract available.
Humans
;
Hypertension*
;
Kidney Failure, Chronic*
;
Renal Insufficiency*
7.The effect of hypertension on the progression of renal insufficiency in chronic renal failure patients.
Dae Suk HAN ; Kyu Hun CHOI ; Young Ki KIM ; Dong Hun CHA ; Ho Yung LEE ; Kyo Sun KIM
Korean Journal of Nephrology 1991;10(2):135-144
No abstract available.
Humans
;
Hypertension*
;
Kidney Failure, Chronic*
;
Renal Insufficiency*
8.Critical Review of Shunting Procedures for Hydrocephalus.
Yoon Sun HAHN ; Hun Joo KIM ; Hun Jae LEE
Yonsei Medical Journal 1976;17(2):163-171
The authors analyzed 174 consecutive shunting procedures for hydrocephalus at Severance Hospital, Yonsei University. There were a total of 65 complications (48.5%) among 134 patients. The most common complications were blockage of the catheters (26.2%) and shunt-related infections (15.4%). Among the different types of shunting technique ventriculoatrial, ventriculoperitoneal and ventriculocisternal routes were most commonly used. The ventriculoatrial route had a greater complication rate (40%) than ventriculoperitoneal shunting (21.8%). Flushing devices are critically reviewed and the disadvantages are discussed. The authors suggested a need for a standardized prospective national data collection system where Procedures and compilations may be assembled in a comparable form.
Bacterial Infections/etiology
;
Catheterization/adverse effects
;
Cerebrospinal Fluid Shunts*/adverse effects
;
Female
;
Human
;
Hydrocephalus/surgery*
;
Infant
;
Male
9.Ten cases of congenital anomalies diagnosed by prenatal ultrasonography.
Keun Hyeoung LEE ; Won Ki OH ; Sun Tae KIM ; Kwang Wook LEE ; Hun Jung IM
Korean Journal of Obstetrics and Gynecology 1991;34(12):1760-1775
No abstract available.
Ultrasonography, Prenatal*
10.Solitary Fibrofolliculoma: A case report.
Hun Kyung LEE ; Lee So MAENG ; Seok Jin KANG ; Soo Il CHUNG ; Sun Moo KIM
Korean Journal of Pathology 1996;30(5):460-462
The clinical and pathological features of solitary fibrofolliculoma are presented. Solitary fibrofolliculoma is very rarely encountered and to our knowledge, only 7 cases have been reported in the Western literature and no cases have been published in Korea. We experienced a case of solitary fibrofolliculoma occurring in a 56-year-old female, who had a 1.0 cm-sized and slowly growing nodule on her chin. A brief review of the literature, was made especially in relation to the pathological findings and histogenesis of solitary fibrofolliculoma.
Female
;
Humans