0.05,there were not significant differences in two groups.In the hypothyroidism group,the characteristics had been improved,such as myxedema、feeling of cold,lowing-sweat、alopeda,The function of thyroid and heart rate had been improved。To compare with matched control,there were significant difference P

Objective To explore the effects of Acer truncatum oil on oxidative stress and inflammatory reaction in rats after exhaustive exercise to provide a scientific basis for the prevention of sports injury by Acer truncatum oil.Methods Thirty healthy male SD rats were randomly divided into control group (A,normal diet + saline every day),exhaustive group (B,normal diet + saline daily) and exhaustive with Acer truncatum oil group (C,0.21 mL/kg daily Acer truncatum oil gavage),6 weeks later,the rats performed once exhaustive swimming exercise,then the creatine kinase (CK),lactate dehydrogenase (LDH),cytokine interleukin (IL-1β,IL-10) and tumor necrosis factor alpha (TNF-α) in the serum;malondialdehyde (MDA) homogenate reduction glutathione (GSH),superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GSH-Px) in muscle were measured.Results After exhaustive exercise,when compared with the group A,the serum levels of CK,LDH,IL-1,TNF-α and IL-10 were significantly higher than in the group B(P＜0.05,P＜0.01,P＜0.05,P＜0.01,P＜0.05),IL-10/ TNF-α ratio had no statistically significant difference(P＞0.05),the level of MDA in gastrocnemius muscle increased significantly (P＜0.05),GSH level and GSH-PX activity decreased significantly (P＜0.05,P＜0.01),SOD and CAT activity were not statistically significant (P＞0.05).when compared with the group B,serum CK,LDH level and IL-1 β,TNF-α were significantly decreased (P＜0.05,P＜0.01,P＜0.01,P＜0.05),IL-10 had no statistical significance (P＞0.05),a significant increase in IL-10/TNF-α(P＜0.05),the level of MDA of gastrocnemius muscle decreased significantly (P＜0.05),the level of GSH and SOD,CAT and GSH-PX were significantly increased (P＜0,01,P＜0.01,P＜0.05,P＜0.01).Conclusion Acer truncatum oil can significantly reduce oxidative stress and inflammatory reaction caused by the exhaustive exercise,which has a protective effect on skeletal muscle.

Objective To explore the relationship between the emotional labor and personality of the health care workers. Methods 168 health care workers in 6 hospitals of Shandong province were surveyed to complete the Emotional Labor Scale and the revised NEO personality inventory(NEO-PI-R). Results The first strategy of emotional labor that workers usually used was natural acting (5.17±1.15),the next one was deep act-ing (4.74±0.78) and the last was surface acting(3.88±1.21). The correlation analysis showed that the extro-version had a negative correlation (r=-0.20, P < 0.05) with the surface acting and a positive correlation (r=0. 31, P<0.01) with the deep acting. The agreeableness had a positive correlation (r=0.22, P < 0.05) with the nat-ural acting. The conscientiousness had a positive correlation (r=0.25, P<0.05) with the deep acting. The extro-version had significant negative influence on surface acting and significant positive influence on deep acting. The a-greeableness had positive significant effect on natural acting and the conscientiousness had positive significant effect on deep acting. Conclusion Personality influenced the emotional labor and gender,age,work tenure and ed-ucational had significant effect on emotional labor.

Objective To study the optimal dose of esmolol for maintaining cardiovascular stability in patients with hypertension during tracheal extubation. Methods In post-anestheisa care unit, hypertensive patients after general anesthesia meeting the extubation criteria were included. Patients were divided into 2 groups according the age: group Ⅰ (>65 years old for the elderly hypertensive, 21 cases), and groupⅡ(≤65 years old for the non-elderly hypertensive, 22 cases). All the patients received esmolol bolus before sputum suction and tube extraction, and the tracheal extubation were extubated 2 minutes after esmolol bolus. The systolic blood pressure, diastolic blood pressure and heart rate were was recorded before tracheal extubation, 2 min after esmolol bolus, at the time of sputum suction extubation, 1 min after tracheal extubation, 3 min after tracheal extubation and 5 min after tracheal extubation. Esmolol dose was determined by the up and down method. Initial dose was 0.5 mg/kg, in accordance with the arithmetic dose (0.2 mg/kg) increasing or decreasing progressively. In negative results (the systolic blood pressure at extubation or 5 min after extubation ≥ 20% of the base, or the systolic blood pressure at sputum suction extubation>180 mmHg, 1 mmHg=0.133 kPa) esmolol dose increased progressively, and in positive results (the systolic blood pressure at extubation or 5 min after extubation<20%of the base) esmolol dose decreased progressively. When the crosspoint (from positive to negative result) reached 6, the study was terminated. Results The median effective doses of esmolol for maintaining cardiovascular stability in groupⅠand groupⅡwere (0.6 ± 0.1) and (0.8 ± 0.1) mg/kg. Conclusions Esmolol can maintain cardiovascular stability in patients with hypertension during tracheal extubation. Median effective dose decreases in older hypertensive patients.

Objective To explore the relationship between humor style and attribution style.Methods A total of 193 college students were studied by means of cluster random sampling with the Students Humor Style Questionnaire and the Multidimensional Multiattributional Causality Scale.Results (1) Self-enhancing humor had positive correlation with internal control,controllability and stability(r =0.293,0.308,0.172; P＜ 0.01).Affiliative humor had no positive correlation with attribution style.Aggressive humor and self-defeating humor had positive correlation with internal control (r =0.146,0.167 ; P ＜ 0.01),external control (r =0.402,0.339 ; P ＜0.01),stability(r=0.286,0.306 ; P＜0.01) and instability(r =0.296,0.227; P ＜ 0.01).(2) Self-enhancing humor could predict internal control and controllability significantly;and aggressive humor and self-defeating humor has significantly positive prediction to stability,instability,internal control and external control.Conclusion Humor style has significant correlation with attribution style.Humor style can predict attribution style in some degree.

Objective To observe the expression and relevance of human papillomavirus L1 (HPVL1)capsid protein and tumor suppressor gene p16~4A in cervical lesions.Methods The expression of HPVL1 capsid protein and p16INK4A in liquid-based cytology specimens and organization from 210 cases infected with HPV virus were detected by Max-vision immunohistochemistry method.Results In different grade of liquidbased cytology specimens,the positive rates of HPVL1 capsid protein had statistic difference (x 2 =70.50,P ＜ 0.005).The rate in LSIL couples was 68 ％ (34/50),which was the highest in all couples.The positive rates of tumor suppressor gene p16INK4A were increased gradually.In SCC couples,the positive rates was 100 ％(30/30),which was the hishest.In LISL couples,the rate of HPVL1+ p16INK4A-was 32 ％ (16/50),which was the hishest.In SCC couples,the rate of HPVL1-p16INK4A+ was 100 ％ (30/30),which was the highest.In orgnizational specimens,the positive rates of HPVL1 capsid protein in cervical intraepithelial neoplasia had statistic difference (x2 =54.37,P ＜ 0.005).The rate in CIN Ⅰ group was 60.4 ％ (32/53),which was the highest.The positive rates of tumor suppressor gene p16 were increased gradually.In cervical cancer group,the positive rate was 100 ％ (28/28),which was the highest.In CIN Ⅰ group,the rate of HPVL1+ p16INK4A-was 45.3 ％ (24/53),which was the highest.In cervical cancer couples,the rate of HPVL1 (-) p16INK4A(+) was 100 ％ (28/28),which was the highest.Conclusion Detection on the expression of HPVL1 capsid protein and tumor suppressor gene p16INK4A can diverse different levels of cervical lesions and separate cases from aggravated or self-healing,to avoid over-treatment or misdiagnose.

Objective To analyze the clinicopathologic features,prognosis and influencing factors of invasive cervical adenocarcinoma.Methods Clinical and pathologic data of 96 cases of invasive cervical adenocarcinoma and 100 cases of squamous carcinoma of the cervix treated in Shanxi Cancer Hospital from years of 1998 to 2007 were retrospectively analyzed.Results The morbidity of cervical adenocarcinoma was showing the arise and low-age trend.The overall 5-year survival rate was 60.4 ％,The overall survival rates of patients with stage Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 82.9 ％,51.5 ％,13.3 ％ and 0,respectively.Ocervical adenocarcinoma had many prognosis-related factors:clinical stage,lymmphatic spread,vascular invasion,volume of tumor,histologic grade.Multivariate analysis showed that clinical stage,lymph nodal metastasis and vascular invasion were independent prognosis-related factors.However,whether ovary was reserved,it was irrelative to prognosis.Conclusion The morbidity of cervical adenocarcinoma is showing the arise and low-age trend.Earlier diagnosis is extremely important for improving the survival rate of cervical adenocarcinoma,the reserved ovary doesn't influence the overall 5-year survival rate for young women with early cervical adenocarcinoma.

Objective To investigate the correlations between obsessive-compulsive symptoms and autism-spectrum quotient and explore if systemizing is the mediator between them. Methods With Padua Inventory (PZ) ,AQ and Systemizing Quotient (SQ), 1901 college students were assessed with stratified random sampling survey. Results Compared with the femal students, score of systemizing in male students had a significant increase ( (26. 773 ± 9.225 )vs (22.017 ± 7 ± 934), P ＜0.01 ). AQ total score was significantly positive correlated with PI total score( r=0. 336, P＜0.01 ),and correlation was also found among SQ,AQ and PI in different degrees. the mediating effect of systemizing was significant. The regression equation was AQ = 18. 942 +0. 064PI -0. 022SQ (P ＜ 0.01 ). Conclusion There are positive correlation between obsessive-compulsive symptoms and AQ in college students, and systemizing is the mediator between above symptoms.

Objective To explore the moderate effect of work control on the work stress-organizational citizenship behavior relationship and work stress-job satisfaction relationship.Methods 200 employees of five corporations in Shandong province were surveyed by questionnaires using work control scale,work stress scale,organizational citizenship behavior scale and job satisfaction scale.Results Work stress was negatively correlated with job satisfaction(r=-0.28,P＜0.01)and organizational citizenship behavior(r=-0.25,P＜0.01).Job satisfaction was significantly higher for employees who perceived high work control under certain work stress(β=0.16,P＜0.05).The relationship between work stress and organizational citizenship behavior was significantly stronger for employees who perceived high work control under certain work stress(β=0.19,P＜0.05).Conclusion The final results showed that work stress Was negatively correlated with organizational citizenship behavior.Work control had a positively moderate effect on the relationship between work stress and organizational citizenship behavior and the relationship between work stress and job satisfaction.

BACKGROUND: Macrophage colony-stimulating factor (M-CSF)/receptor activator of nudeer kappa B ligand (RANKL), two types of cytokines co-induce myeloid stem cells to form osteoclasts, is a kind of new method to harvest ostaoclasts with high purity and quantity, but there is lack of uniform cultivation standard. OBJECTIVE: To construct an effective M-CSF/RANKL induced mice myeloid stem cells inducing osteoclast differentiation cultivation system. METHODS: Myeloid stem cells ware obtained from ICR mice and then cultured for 24 hours in a-minimum essential medium containing M-CSF, at cell density of 10~7/L, 10~8/L, 10~9/L. Then 10 μg/L M-CSF and 20, 50, 100 μg/L RANKL were added into culture medium. Tartaric-resistant acid phosphatase stained was performed to observe the transition process from stem cell to osteoclast, as well as cell morphology and stain situation after culture, and positive stained osteoclasts were counted. We compared the influence of different induction conditions to the quantity of osteoclast. RESULTS AND CONCLUSION: A small quantity of osteoclasts contained many red positive beads in the intracytoplasm were observed at 3 days. There were positive beads with hypochromatic dikeryon in cells. A large amount of positively stained osteoclests were seen after 6-day cultudng, which maintained dikaryon. After 9-day culturing, positively stained colossal multinudear cells occurred, became larger and maintained three nuclei. At certain cell density, 100 μg/L RANKL could induce to form more osteoclasts compared with other 2 concentrations (P < 0.05); at certain RANKL concentration, the osteoclasts formation at cells density of 10~8/L was dramatically greater than other 2 cell densities (P < 0.05); the number of osteoclasts was the most when the concentration of RANKL was 100 μg/L and cell density of 10~8/L (P < 0.05). When osteoclasts are induced by M-CSF/RANKL from mudne myeloid stem cells, the best concentration of RANKL is 100 μg/L and cells density is 10~8/L.