OBJECTIVE: To evaluate the diagnostic value of polymerase chain reaction (PCR) as opposed to Enzyme immunoassay (EIA) of Chlamydia trachomatis in cervices of women with genital tract infection. METHODS: Sixty three samples (60 cervical samples and 3 pelvic abscess samples) from sixty women with genital tract infection or lesions such as vaginits, cervicitis, cervical intraepithelial neoplasm (CIN), condyloma accuminata, tuboovarian abscess, preterm labor and premature repture of membranes were tested for Chlamydia trachomatis with PCR and EIA simultaneously. The primers for PCR were for 478 base pairs encoding Chlamydia cryptic plasmid gene RESULTS: Six cases (seven samples) (10%) out of 60 women were positive on PCR while one case (1.7%) was positive on EIA among these women. The positive rate of PCR Chlamydia test according to the diagnosis was as followings; 3 out of 19 cases (15.8%) of cervicitis, 2 out of 5 cases (40%) of CIN, 1 out of 3 cases (33.3%), ol tuboovarian abscess and 1 out of 7 cases (14, 3%) of preterm premature rupture of membranes showed positive Chlamydia test. CONCLUSIONS: Diagnostic testing of Chlamydia using PCR showed more sensitive for detection of Chlamydia trachomatis compared with EIA in women with genital tract infection. So Chlamydia PCR test is suggested to be valuable in appropriate treatment and prevention of Chlamydia infection especially in women with cervical lesions and pelvic inflammatory disease.
Abscess ; Base Pairing ; Cervical Intraepithelial Neoplasia ; Chlamydia Infections ; Chlamydia trachomatis* ; Chlamydia* ; Diagnosis ; Diagnostic Tests, Routine ; Female ; Humans ; Immunoenzyme Techniques ; Membranes ; Obstetric Labor, Premature ; Pelvic Inflammatory Disease ; Plasmids ; Polymerase Chain Reaction* ; Pregnancy ; Reproductive Tract Infections ; Rupture ; Uterine Cervicitis

No abstract available.
Female ; Hypertension, Pregnancy-Induced* ; Iron* ; Pregnancy ; Pregnancy*

No abstract available.
Chorion* ; Chorionic Gonadotropin* ; Female ; Humans ; Laparotomy* ; Pregnancy ; Pregnancy, Tubal*

With the emergence of glycomies in the post-genome era,various lectin-related techniques have been developed to analyse the glycoproteins from cells,body fluids and tissues in comparative glycoprotein studies of cancer research.Compared to other techniques of glycan enrichment and identification,lectin-based techniques can sort and identify glycoconjugates without glycan modification,ascribed to the specific affinity of lectins and their corresponding glycans.

OBJECTIVE: To assess the correlation of erythropoietin(EPO) levels and number of nucleated erythrocytes(NRBC) with measurements of blood gases and to investigate the obstetrical factors influencing the levels of EPO and number of NRBC in umbilical venous blood of non asphyxiated neonates. METHODS: Measurements of EPO levels by RIA, number of NRBC per 100 white blood cells(WBC) by blood smear and measurement blood gases were performed at delivery in umbilical venous blood from 117 cases of neonates with gestational age ranged from 29 to 42 weeks, including 77 cases of non-asphyxia and 40 cases of asphyxia. Statistcial analysis included Student's t test, linear regression and linear correlation using SPSS 7.0 version statistical package. RESULTS: The umbilical venous EPO level is correlated significantly with the number of NRBC (r2 =0.204, p<0.05). The level of EPO as well as the number of NRBC showed inverse correlation with umbilical venous pH(r=-0.223, P<0.05 ; r=-0.896, p<0.05). Number of NRBC showed correlation with pCO2 (r=0.700, P<0.01) and base deficit (r=0.316, p<0.01). The mean level of EPO in non-asphyxiated neonates was 26.2 +/-16.1 mIU/mL showing correlation with gestational age and birth weight and higher level in neonates delivered vaginally than those delivered by elective cesarean section (27.1+/-17.0 vs 18.8+/-6.7 mIU/mL, p=0.012). The mean number of NRBC in non-asphyxiated neonates was 2.5+/-2.1/100 wbc showing no difference according to mode of delivery, gestational age and birth weight. CONCLUSION: Number of NRBC not only correlated with cord gas parameters more strongly but also was less influenced by the obstetrical factors than EPO level.
Asphyxia ; Birth Weight ; Cesarean Section ; Erythroblasts* ; Erythropoietin* ; Female ; Gases* ; Gestational Age ; Humans ; Infant, Newborn ; Linear Models ; Pregnancy

Objective:To investigate the effects of sodium phenylbutyrate(NaPB) on the matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW-1.Methods: CGTHW-1 cells were treated with different concentrations of NaPB,then the invasive ability of CGTHW-1 cells was assessed using Transwell assay.The expression of MMP-9 and TIMP-1 was examined by immunocytochemistry staining and RT-PCR in CGTHW-1 cells.Results: After treatment with NaPB(4 mmol/L) for 72 h,CGTHW-1 cells passing the Transwell were significantly reduced[(29.8?1.77) vs(11.00?2.59),P

Glutathione S-transferase kappa 1 (GSTK1) is a key regulator for adiponectin secretion and multimerization.In Caenorhabditis elegans,GSTK1 is involved in energy production and lipid metabolism.Meanwhile,the GSTK1 level is negatively correlated with obesity.It may alleviate the endoplasmic reticulum stress-mediated downregulation of adiponectin.Moreover,a polymorphism in human GSTK1 promoter is related with insulin secretion and fat deposition.Therefore,GSTK1 might be a novel target for the treatment of insulin resistance and the relevant metabolic diseases.

Adult ; Female ; Glioma ; pathology ; surgery ; Humans ; Neoplasms, Multiple Primary ; pathology ; surgery ; Ovarian Neoplasms ; pathology ; surgery ; Peritoneal Neoplasms ; pathology ; surgery ; Teratoma ; pathology ; surgery

Female ; Humans ; Infant ; Nasopharyngeal Neoplasms ; pathology ; Teratoma ; pathology

No abstract available.
Laser Therapy*