Objective To investigate the effects of progesterone on apoptosis and Survivin expression protein of Ishikawa cell line of human endometrial carcinoma in vitro.Methods The proliferation of Ishikawa cells in vitro was determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle,apoptotic percentage were detected by cytometer. The expression of Survivin protein of Ishikawa cells was detected by immunocytochemistry. Results Progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone reached at 0.5?10-5～10-4mol/L(P

Objective To investigate the effect of hindlimb unloaded 3 weeks on bone mineral density of different parts in simulated weightlessness rats,and the effect of Chinese herbal prescriptions on them.Methods 2-month-old male Wistar rats were randomly divided into 3 groups:control group(CON),hindlimb unloading group(HU),HU+Chinese herbal prescription group(HUC).During the whole experiment(4 weeks),HUC were given Chinese herbal prescriptions(contains Radix Acanthopanacis Senticosl,Radix Rehmanniae Praeparata,Radix Achyranthis Bidentatae,Concha Ostreae,etc),while CON and HU were given equal measures Concha Ostreae prepared by acetic acid.After 1 week of adaption period,weightlessness was simulated by tail suspension in HU and HUC for three weeks.Rats were executed at the end of the fourth week and bone mineral density of 7 differernt parts were determined.BMD of skull bone and second thoracic vertebra were examined by buoyancy method,and fourth lumbar vertebra,humerus,femur,radio-ulna and tibia were examined by bone densitometer.Results Compared with CON,BMD of skull of HU increased significantly(P 0.05),but BMD of lumbar vertebra,femur and tibia were decreased notably(P 0.05).Conclusions The change of hindlimb unloaded 3 weeks on systemic BMD presented certain trend.BMD of skull bone increased,little change on upper limbs and second thoracic vertebra,and fourth lumbar vertebra and hind limbs reduced.Chinese herbal prescriptions had the function to relieve weightlessness osteoporosis,and had positive effect on calcium distribution with weightlessness.

UNLABELLEDTo study the effect of a Chinese herbal prescription on external calcium deposition to weight-bearing bone in simulated weightlessness rats.

METHODTwenty-one male Wistar rats were divided into 3 groups: control group, tail suspension group, tail suspension with Chinese medicine group which takes a Chinese herbal prescription extract (containing Radix Rehmanniae Preparata, Radix Acanthopanacis Bidentatae, Radix Astragali, Radix Angelicae Sinensis, Concha Ostreae prepared by acetic acid) by intragastric administration. After 1 week adaption, there start off 3 weeks simulated weightlessness by tail suspension. At the eleventh day of simulated weightlessness, every rat was given one equal dose of 41Ca tracer by intragastric administration. Right femurs were separated as experiment over, and the ratio of 41Ca to 40Ca (41Ca/40Ca) was measured by accelerator mass spectrometry (AMS), while total femur calcium was measured by inductively coupled plasma-atomic emission spectroscopy (ICP-AES). Femur 41Ca deposition amount (DA) and femur 41Ca deposition ratio (DR) were calculated.

RESULTThe results showed that compared with control group, 41Ca/40Ca decreased significantly (P < 0.001) in tail suspension group, while in tail suspension with Chinese medicine group, it significantly increased (P < 0.05). DA and DR were both decreased significantly (P < 0.001) in tail suspension group, but no significant change in tail suspension with Chinese medicine group as compared with control group. Compared with tail suspension group, DA and DR increased significantly (P < 0.001) in tail suspension with Chinese medicine group.

CONCLUSIONSimulated weightlessness by tail suspension can cause decreased deposition of external calcium to weight-bearing bone, and the Chinese herbal prescription in this trial is effective to prevent the decrease. Moreover, multiple mechanisms may contribute to weightlessness induced osteoporosis, besides calcium deposition disturbance.

Animals ; Bone Resorption ; etiology ; metabolism ; Calcification, Physiologic ; drug effects ; Calcium ; metabolism ; Calcium Radioisotopes ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Femur ; drug effects ; metabolism ; Hindlimb Suspension ; Male ; Mass Spectrometry ; Rats ; Rats, Wistar ; Weightlessness Simulation ; adverse effects