The application and expanding of nasal endoscopic surgery,an epoch-making transform of rhinology,have a very close associations with medical imaging,which includes anatomy photograph,conceptual diagram,radiological imaging,nasal endoscopic image display system,operation photographic recording and computer assisted instruction courseware,etc.It is important to collect the medical imaging through various means and set up medical imaging resources library for nasal endoscopic surgery teaching.

On the basis of experiences in advanced otolaryngology-head & neck surgeons teaching,we realized that it was important to accomplish the following measures:to insist on entering instruction,to make reasonable training plan,to hold regular lecture,to attach importance to applied anatomy teaching,to cultivate clinical ability,to standardize medical record writing,to strengthen communication between doctors and patients and to strengthen medical morality construction.Only by doing so can we improve the advanced education quality.

OBJECTIVE: Polymorphism of interleukin-23 receptor (IL-23R) gene was found being related with the susceptibility to several immune-related diseases. For the terminal differentiation of IL-17-producing effecter T-helper cells in vivo, the IL-23R gene is very important. As proved recently, Th17 cells might have a great influence to the pathogenesis of allergic airways disease. Our intention was paid to find any association between the polymorphisms in the IL-23R gene and allergic rhinitis(AR) in Chinese population. METHOD: A group of patients was involved in a case-control comparison, consisted of 239 AR patients and 271 control Chinese subjects. The study claimed to take some blood samples for DNA extraction and select 3 single-nucleotide polymorphisms in IL-23R individually genotyped by the PCR-RFLP method. RESULT: Comparison with the controls, a great growing prevalence of the homozygous rs7517847 GG genotype and G allele appeared in AR patients (P < 0.05, respectively). Besides, a great increased frequency of the GGA haplotypes was presented in AR patients in comparison with the controls (P < 0.05). CONCLUSION The results demonstrated a important association pattern between polymorphisms in IL-23R and AR in Chinese population. Between rs7517847 in an SNP of IL-23R and AR, a great association was identified.
Adolescent ; Adult ; Aged ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Genetic Predisposition to Disease ; Genotype ; Haplotypes ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Receptors, Interleukin ; genetics ; Rhinitis, Allergic ; genetics ; Young Adult

OBJECTIVE: To study the inhibitory effects of short hairpin RNA (pshLMP1) combined with DNAzyme or alone on latent membrane protein-1 (LMP1)expression in HNE1 cell lines. METHOD: The pshLMP1 and DNAzymes were co-transfected with pEGFP-N1-1158 (a expression vector encoding LMP1 and EGFP fusion protein) into HNE1 cells, divided into 4 groups as positive control, RNA interference, combining and DNAzyme groups. The green fluorescence were analyzed and then the mRNA and protein of LMP1 gene were detected. RESULT: The HNE1 cells expressing green fluorescence in combining group were significantly less than that in RNA interference group (P < 0.01); The inhibition efficiency of green fluorescence in combining group were 86. 31% Wp 88.88% ,which were higher than that in RNA interference group with 75.15%. The detection of LMP1 mRNA and proteins showed that combining group had a higher inhibition ability. CONCLUSION Combining gene therapy with pshLMP1 and DNAzyme is superior to pshLMP1 alone to inhibit LMP1 gene expression.
Cell Line, Tumor ; DNA, Catalytic ; therapeutic use ; Exons ; Gene Expression Regulation, Viral ; Genetic Therapy ; Humans ; Nasopharyngeal Neoplasms ; genetics ; metabolism ; pathology ; RNA, Small Interfering ; genetics ; therapeutic use ; Transfection ; Viral Matrix Proteins ; genetics

OBJECTIVE: To probe into both the quality of life and personality characteristics of allergic rhinitis (AR) patients. METHOD: AR patients were investigated by questionnaires of SRF-36, EPQ, SAS, SDS, then the influencing factors were analyzed by multiple regression analysis. RESULT: People suffered from AR were influenced on quality of life, especially on role-physical, general health, role-emotional; there was no obviously difference on personality characteristics; however, anxiety tendency but no depressive emotion was observed. CONCLUSION In order to seek better treatment under new medical model, we should put emphasis on the quality of life and personality characteristics of AR patients.
Adolescent ; Adult ; Asthma ; epidemiology ; psychology ; Female ; Humans ; Male ; Middle Aged ; Quality of Life ; Rhinitis, Allergic, Perennial ; epidemiology ; psychology ; Surveys and Questionnaires ; Young Adult

OBJECTIVE: To detect and analyze the EBV-LMP1 gene N terminus XhoI-site mutation and C terminus 30 bp deletion in plasma of patients with nasopharyngeal carcinoma (NPC) in Chongqing of China. METHOD: DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China. All the PCR production of N terminus was digested by enzyme XhoI, then segregated in 8% PAGE. All- the PCR production of C terminus was segregated in 8% PAGE too. Compared with B95-8 cell, some of the PCR production were sequenced and analyzed with software. RESULT: LMP1 was amplified successfully from 48 of 48 NPC cases (100%) and from 38 of 40 non-NPC cases (95%) by PCR, but none of LMP1 XhoI-site mutation and none of 30 bp deletion was found by digesting, segregating and Sequencing. CONCLUSION EBV-LMP1 gene N terminus XhoI-site and C terminus 30 bp deletion have no mutation in plasma of NPC or non-NPC cases in Chongqing of China. The precise relationship of EBV-LMP1 gene mutation or deletion with NPC pathogenesis needs further investigation.
Adult ; China ; DNA, Viral ; genetics ; Female ; Herpesvirus 4, Human ; Humans ; Male ; Middle Aged ; Nasopharyngeal Neoplasms ; blood ; genetics ; pathology ; virology ; Sequence Deletion ; Viral Matrix Proteins ; blood ; genetics

Objective To discuss the application of mind map in self-management of elderly patients after receiving mitral valve transcatheter edge-to-edge repair(M-TEER).Methods A total of 66 patients,who underwent M-TEER at Nanjing Hospital of Nanjing Medical University from August 2021 to October 2022,were enrolled in this study.Using the envelope concealment method,a total of 66 data analysis samples were included in the analysis.There were 33 patients each in the study group and control group.Routine health education was adopted for the patients of the control group,while the responsible nurse conducted health education for the patients of the study group under the guidance of the mind map that was designed by a multidisciplinary specialized nurses.Results There was a significant difference in postoperative medication compliance between the study group and the control group(P＜0.05),i.e.the degree of compliance,including taking medicine on time,insisting on taking medicine and taking medicine as prescribed,in the study group was obviously higher than that in the control group.The postoperative 6-min walking test,which was regarded as one of the indicators of cardiac functions,in the study group was remarkably better than that in the control group,the difference was statistically significant(P＜0.05).The postoperative quality of life(including daily activity ability,frailty degree,social support,and incidence of hospitalization for heart failure within one year after treatment)in the study group was strikingly better than that in the control group(P＜0.05).Conclusion The use of mind map in self-management of elderly patients after receiving M-TEER can effectively improve the medication compliance of the patients after discharge,improve the quality of life of patients,and reduce the incidence of hospitalization due to heart failure.