1.The expression of interleukin-25 in blood and nasal tissue of patients with chronic rhinosinusitis with nasal polyps.
Zhijuan GAO ; Jianting WANG ; Hongying ZHAO ; Suliang GUO ; Xiuxia HUANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(20):1598-1601
OBJECTIVE:
To explore the expression of interleukin-25 (IL-25) in chronic rhinosinusitis with nasal polyps (CRSwNP) and its potential significance in pathogenesis.
METHOD:
IL-25 expression in blood was detected by enzyme-linked immunosorbent assay (ELISA). IL-25 expression in tissue was detected by immunohistochemistry (LSAB method) from polyps (68 CRSwNP patients) and 55 inferior turbinate mucosa from patients with deviation of nasal septum served as control. Complete blood count and HE staining of blood and tissue eosinophil infiltration degree.
RESULT:
IL-25 expression in CRSwNP group were significantly higher than the control group, the difference was statistically significant (P < 0.01). IL-25 expression in local organizations was positively correlated with the number of eosinophil infiltration in CRSwNP group (r = 0.679, P < 0.01).
CONCLUSION
The expression of IL-25 in CRSwNP patients mutually reinforcing and might increase eosinophil infiltration and play an important role in the development of CRSwNP.
Chronic Disease
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Interleukin-17
;
blood
;
Nasal Polyps
;
blood
;
Nasal Septum
;
Rhinitis
;
blood
;
Sinusitis
;
blood
;
Turbinates
2.Molecular identification and typing of 182 strains of Streptococcus pneumoniae isolated from children
Yinghui GUO ; Baohua HE ; Yingtong WANG ; Zhaoyi JIA ; Qian WANG ; Guixia LI ; Wenchao ZHANG ; Yinqi SUN ; Suliang CHEN
Chinese Journal of Infection Control 2017;16(4):326-329
Objective To type Streptococcus pneumoniae(S.pneumoniae) isolated from children, and provide scientific basis for the correct selection of S.pneumoniae vaccine.Methods 182 strains of S.pneumoniae were collected from Children's Hospital of Hebei Province in 2014, species of strains were identified by polymerase chain reaction (PCR), types of strains were analyzed with multiplex PCR.Results PCR detection showed that cpsA gene amplification of 182 strains were all positive;multiplex PCR detection revealed that except 8 strains were not typed, the main types of the remaining 174 strains were 19 F (n=68, 37.36%), 19A(n=33, 18.13%), and 6A/6B (n=26,14.28%), the other types were 35B, 14, 6C/6D, 23F, 15B/15C, and so on.Conclusion The main types of 182 strains of S.pneumoniae are 19 F, 19A, and 6A/6B, which provide scientific basis for the correct selection of S.pneumoniae vaccine for this province.