BACKGROUND: At the process of wound healing, the epithelial cells and epidermal stem cells interactions is complicated which promoting the mechanisms of wound healing. The abnormities of any fine phase can be result in delayed healing or scarring. OBJECTIVE: To summarize the characterization of epithelial cells and epidermal stem cells and their interaction in wound healing so that we can recognize the mechanisms of scarring from histomorphology, biochemistry and molecular variations. METHODS: A computer-based online search of Pubmed Database was undertaken to identify the relevant articles on epithelial cell and epidermal stem cells interactions in wound healing and results in scarring published from 1974 to 2009 with the key words of "epithelial cells, epithelial stem cells, wound healing, keratinocytes, fibroblasts, hypertrophic scar, keloid" in English. At the same time, Chinese relevant articles were searched in China Journal Full-text Database (CJFD) published between 1999 and 2009 with the same key words in Chinese. A total of 43 articles were collected about the cell structure (3 articles), the cell interactions in wound healing and scarring (16 articles), the relevant on epithelial stem cells (24 articles).RESULTS AND CONCLUSION: The effect of different cells in epithelial tissue is known to all. The keratinocytes and fibroblasts had been on studying frequently, and their closely correlated to scarring. At present, the epithelial stem cells has been confirmed, but the relevant on their location, quality and specific epidermal markers are unclear which need to study and explore. With the rapidly development of science and technology, we maybe control the expression of cells by genic regulation not only culture the cells of our need but to prevent the scarring.

OBJECTIVE:To observe therapeutic efficacy of adjuvant treatment of Xuebijing injection for severe acute pancreati-tis. METHODS:80 cases of severe acute pancreatitis were randomly divided into control group and observation group with 40 cas-es in each group. Control group was given symptomatic and supportive treatment,and observation group was additionally given in-travenous injection of Xuebijing injection 100 ml,twice a day,on the basis of control group. The levels of TNF-α,IL-6 and hs-CRP,organ failure were compared between 2 groups before and after treatment. RESULTS:There was no statistical significance in the levels of TNF-α,IL-6 and hs-CRP in 2 groups before treatment(P>0.05);after treatment,the levels of TNF-α,IL-6 and hs-CRP in observation group was significantly lower than control group and before treatment,with statistical significance (P<0.05). The proportion of renal function,respiration function and circulating failure in observation group were significantly lower than in control group,with statistical significance (P<0.05). CONCLUSIONS:Adjuvant treatment of Xuebijing injection for se-vere acute pancreatitis can significantly reduce the level of inflammation in the body and reduce organ damage.

Objective To study the efficacy and safty of low dose rituximab therapy in elderly patients with chronic refractory immune thrombocytopenia(ITP) .Methods 36 elderly patients with chronic refractory ITP were collected from Xingtai People′s Hospital of Hebei ,the patients were given intravenous injection of intravenous rituximab at the dose of 100 mg once weekly for 4 consecutive weeks ,observing the efficacy and adverse reaction and following up for 12 months .Difference of platelet (PLT ) ,white blood cell (WBC) ,cluster differentiation 8 + (CD8 + ) ,CD19 + eukomonocyte number and immunoglobulin (IgG ,IgM ,IgA )were compared before and after treatment .Results The responses of complete remission (CR)were 17 casese(47 .2% ) ,remission (R) were 11 casese (30 .6% ) and no remission (NR) 8 casese (22 .2% ) respectively ,2 patients experienced mild dizziness and chest tightness ;PLT were significantly increased ( P = 0 .001) after treatment ,CD19 + lymphocyte count decreased significantly (P =0 .001) ,WBC ,CD8 + lymphocyte and serum immunoglobulin IgG ,IgM ,IgA showed no significant difference before and after treat-ment(P> 0 .05) .Conclusion Low dose rituximab had better clinical efficacy for the treatment of elderly patients with chronic re -fractory ITP ,low dose rituximab may be a effective treatment in elderly chronic refractory ITP patients with minor adverse reac -tions .

Thyroid peroxidase(TPO) gene was detected in 20 patients with congenital hypothyroidism. An insertion c. 2268insT of TPO gene was found in one of them, and c. 2268insT combined with c. 1477G＞C mutation in another. TPO gene mutation may be related to pathogenesis of congenital hypothyroidism in Chinese.

Objective To evaluate whether the forced expiratory volume in sixth scend(FEV6) was the optimal surrogate for forced vital capacity (FVC) in the diagnosis for chronic obstructive pulmonary disease (COPD).Methods Retrospectively analyzed the spirometric data of 142 COPD patients (7 cases with mild COPD,60 cases with moderate COPD,48 cases with severe COPD,27 cases with extremely severe COPD) admitted to Hebei Chest Hospital from October 2011 to October 2012 who had FEV6 data.FEV6,FEV5,FEV4 and FEV3 were measured on volume-time curves and the diagnostic value was analyzed.Results FEV6,FEV5,FEV4 and FEV3 were highly correlated to FVC (r =0.994,0.939,0.935 and 0.923 respectively,P ＜0.001).Assuming =70％ as the diagnostic standard for obstruction,FEV1/FEV6 had a diagnostic rate of 92.96％ with a false negative rate of 7.04％.FEV1/FEV5,FEV1/FEV4 and FEV1/FEV3 had higher false negative rates (9.86％,13.38％ and 27.46％ respectively) than FEV6.The value of FEV1/FEV6-FEV1/FVC had no statistical significance between the mild-moderate and the severe-extremely severe COPD groups(t =1.376,P =0.171).Conclusion There is a strong correlation between FEV6 and FVC.FEV6 may be the best surrogate for FVC in the diagnosis of chronic obstructive pulmonary disease.Elevating diagnostic critical value can reduce the false negative rate.

Adolescent ; Adult ; Female ; Humans ; Male ; Occupational Diseases ; etiology ; prevention & control ; Occupational Exposure ; analysis ; prevention & control ; Trichloroethylene ; poisoning ; Young Adult

Objective:To study relationship between B10 cells and the incidence of autoimmune diabetes in nonobese diabetic mice. Methods:20 NOD/LT female mice of 6 week old were cultured in normal culture to 30 weeks,and the mice were divided into two groups according the mice’s blood glucose,serum creatinine and body weight detected at their 30 weeks old. IL-10 levels in spleen tissues of the two groups were detected by enzyme-linked immunosorbent assay. We used flow cytometry to detect the proportion of B10 cells in the spleen of mice in the two groups. NOD/LT mice were randomly divided into control group and B10 group. The B10 cells were inoculated in B10 groups,their blood glucose were detected when they were 10,15,20,25 and 30 weeks old. Results: The blood glucose and serum creatinine levels were significantly higher in the group than that in the autoimmune diabetes group (P< 0. 05),and the body weight was significantly lower than that in the autoimmune diabetes group (P<0. 05). The level of IL-10 in the spleen tissues of the autoimmune diabetes mice was significantly higher than that in the non autoimmune diabetes group. The content of B10 cells in the spleen of the mice with autoimmune diabetes mellitus was significantly higher than that in the non autoimmune diabetes group. When mice at the age of 10,15 weeks,the incidence of autoimmune diabetes in B10 group was significantly lower than that in the control group,but the incidence of autoimmune diabetes in B10 group was significantly higher than that in control group at 20,25 and 30 weeks. Conclusion:The over accumulation of B10 cells may be one of the reasons for the further development of autoimmune diabetes in NOD mice.

Objective To explore and compare the clinical efficacy of levetiracetam tablets and compound sodium valproate sustained release tablets in the treatment of children and adolescents with epilepsy.Methods From April 2017 to April 2018,80 children and adolescents with epilepsy treated in Chaonan Minsheng Hospital of Shantou were selected as study objects,and they were randomly divided into two groups by drawing lots,with 40 cases in each group.The observation group was given levetiracetam tablets,and the control group was treated with valproate.The improvement of EEG after therapy,the total effective rate,and the incidence of adverse reactions were observed and evaluated.Results The EEG improvement rates after treatment for 6 months in the observation group and control group were 41.17％,45.71％,respectively,the difference was not statistically significant(x2 =0.508,P ＞0.05).The EEG improvement rates after treatment for 9 months in the observation group and control group were 70.58％,74.28％,respectively,the difference was not statistically significant (x2 =0.225,P ＞ 0.05).The total effective rate in the observation group was 92.50％,which was 95.00％ in the control group,the difference was not statistically significant between the two groups (x2 =0.354,P ＞ 0.05).However,the incidence rate of adverse reactions of the observation group(22.50％) was significantly lower than that of the control group(45.00％)(x2 =6.864,P ＜ 0.05).Conclusion Both levetiracetam tablets and compound sodium valproate sustained release tablets have appreciable efficacy and safety in the treatment of epilepsy in children and adolescents,but levetiracetam therapy has less adverse reactions,which deserves further promotion in monotherapy of epilepsy in children and adolescents.

Objective:To investigate different sleep duration and glucose and lipid metabolism levels in residents of a community in Urumqi.Methods:Using the 2 049 residents′ data of chronic metabolic disease in a community of Urumqi collected in May 2017, 1 822 subjects aged between 19-80 years with complete information were enrolled, their blood pressure, waist circumference, height, weight, body mass index were measured and recorded. Using oral glucose tolerance test to measure fasting and 2 h after meal plasma glucose, uric acid, HbA 1C, total cholesterol, triglyceride, low density lipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C) levels were all tested. Results:(1)There were 363 (19.9%), 1 349 (74.0%), and 110 (6.1%) respondents with sleep time≤6.0, 6.1 to 8.0, and>8.0 h/d, respectively. There were statistically significant differences in age, education, and family income in groups with different sleep time ( P<0.05), while their gender, smoking status, and exercise status were not statistically significant ( P>0.05). The rates of overweight, obesity, abdominal obesity, high uric acid, and hypertension in people with different sleep durations were statistically different ( P<0.01). The rates of the above indicators were higher in the group of sleep time≤6.0 h/d than the other two groups. (2) Differences in diastolic blood pressure, systolic blood pressure, body mass index, abdominal circumference, total cholesterol, and LDL-C levels were statistically significant among different sleep duration groups ( P<0.05). Further comparisons of the above indicators among three groups with different sleep durations were performed ( P<0.05). The levels of the above indicators in the sleep time≤6.0 h/d group were higher than those in the other two groups. There were no significant differences in fasting blood glucose, glycated hemoglobin, uric acid, triglyceride, and HDL-C among the three groups. (3) Multivariate logistic regression analysis showed that groups whether or not adjusted of age, family income, and education level, sleep time≤6.0 h/d was related to abdominal obesity, and sleep time≤6.0 h/d was be a risk factor for abdominal obesity [Unadjusted: OR=1.48(95% CI1.04-2.08); Adjusted: OR=1.65(95% CI1.18-2.32; P<0.05]. Conclusion:Sleep time ≤6.0 h/d is associated with abdominal obesity, and sleep time≤6.0 h/d may be a risk factor for abdominal obesity.

Objective To investigate the clinical value of Xpert M TB/RIF in the early diagnosis of tuberculous meningitis (TBM ) .Methods Totally 130 patients with central nervous system infection in our hospital from February 2015 to December 2016 were divided into two groups ,65 cases of TBM patients as the TBM group ,65 cases of non TBM patients as the non TBM group . The CSF samples of all patients were respectively detected by acid fast staining ,Roche solid culture and Xpert MTB/RIF assay .The test results were compared .Results With clinical diagnosis as the gold standard ,the sensitivity of Xpert MTB/RIF to detection of TBM was 43 .08% and specificity was 100 .00% .The sensitivity of solid culture to detection of TBM was 58 .46% and specificity was 98 .46% .The sensitivity of acid fast staining to detection of TBM was 9 .23% and specificity was 100 .00% .Based on the re-sults of drug sensitivity test of traditional proportional method ,the sensitivity of Xpert M TB/RIF to detection of rifampin resistance was 88 .89% and specificity was 98 .35% .Conclusion Xpert M TB/RIF is a new diagnostic technique for detecting TBM and ri-fampin resistance in patients .It has the advantages of rapid ,direct ,reliable and high specificity and is worthy of clinical application .