sulfuric acid and phosphoric acid, 24 (group 2 GC) were prepared at galvanostatic mode in calcium glycerophosphate and calcium acetate and 24 (group 3 CMP (Calcium Metaphosphate) Coating were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid followed by CMP coating. Rest of 24 (control group were as a control group of RBM surface. Bone tissue responses were evaluated by resonance frequency analysis (RFA) that were undertaken at 2, 4 and 6 weeks after implant placement in the mandible of mini-pig. Group 1 SP (anodized with sulfuric acid and phosphoric acid implants) demonstrated slightly stronger bone responses than control Group RBM. Group 2 GC (anodized surface with calcium glycerophosphate and calcium acetate implants) demonstrated no difference which were compared with control group. Group 3 GMP (anodized and CMP coated implants) demonstrated slightly stronger and faster bone responses than any other implants. But, all observation result of RFA showed no significant differences between experimental groups with various surface type. Histomorphometric evaluation demonstrated significantly higher bone-to-implant contact for group 2 GC. Significantly more bone formation was found inside threaded area for group 2 GC. It was concluded that group 2 GC (anodized surface with calcium glycerophosphate and calcium acetate implants) showed more effects on the bone tissue responses than RBM surface in initial period of implantation. In addition, CMP showed a tendency to promote bone tissue responses.]]>
Acetates ; Bone and Bones ; Calcium ; Calcium Compounds ; Glycerophosphates ; Mandible ; Osseointegration ; Osteogenesis ; Phosphoric Acids ; Sulfur ; Sulfuric Acids ; Titanium

The pure titanium disks were divided into three groups and etched for 30 minutes with HNO3, hot H2SO4/H2O2 or hot H2SO4/HCl respectively. The treated disks were studied and analyzed with scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). The disks etched with HNO3 had a smooth surface, while those etched with hot H2SO4/H2O2 or hot H2SO4/HCl had rough surfaces, and the surface etched with hot H2SO4/HCl had larger micropores. The XPS analysis demonstrated that the main elements of the surface in three groups were titanium, oxygen and carbon. The carbon concentration was the lowest on the surface etched with hot H2SO4/H2O2 and the highest on that etched with hot H2SO4/HCl. The substances were TiO2, Ti2O3, TiO and metal Ti on the surface etched with HNO3 or hot H2SO4/H2O2. Only TiO2 was detected on the surface etched with hot H2SO4/HCl.
Hydrochloric Acid ; Hydrogen Peroxide ; Metallurgy ; methods ; Nitric Acid ; Oxides ; Sulfuric Acids ; Surface Properties ; Titanium ; chemistry

This study was aimed to establish an applicable, convenient and rapid method for platelet intracellular trehalose determination, so that the technique of trehalose loading could be optimized and used in research on freeze-drying platelets. Protein from loaded-trehalose platelets was deposited by using trichloroacetic acid, trechalose concentration in platelets was determinated by sulfuric-anthrone reaction and was analyzed by high performance liquid chromatography (HPLC). The results showed that when platelets were loaded with 1.7% of trehalose in loading solution, intracellular concentration determinated by sulfuric-anthrone reaction was 0.22% and derterminated by HPLC was 0.2%. The recovery rate of trehalose determinated by sulfuric-anthrone reaction was 100.7% and stability and repeatability of results were good. In conclusion, the method is convenient, rapid, accurate and highly sensitive for determination of platelet intracellar trehalose.
Blood Platelets ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Humans ; Reproducibility of Results ; Sulfuric Acids ; chemistry ; Trehalose ; analysis

OBJECTIVETo study the production technology of extraction in combination hydrolysis in situ for isolating diosgenin from Dioscorea nipponica by orthogonal design.

METHODThe optimum production conditions were investigated with the recovery of diosgenin as an index by extraction in combination hydrolysis in situ, and were compared with the traditional method.

RESULTExtraction in combination hydrolysis in situ conducted in 1.5 mol x L(-1) sulfuric acid of water containing 75% isopropanol at 100 degrees C for 4.5 h could get higher recovery of diosgenin than traditional methods.

CONCLUSIONThis production technology can get higher recovery of diosgenin, and it is simple, time and money saving.

Dioscorea ; chemistry ; Diosgenin ; isolation & purification ; Hydrolysis ; Pressure ; Sulfuric Acids ; Technology, Pharmaceutical ; methods ; Temperature ; Time Factors

It has been submitted that different ion solutions containing sulfate induce crystal growth and might substitute conventional acid etching for pretreatment of enamel in orthodontic bonding(Artun et al., Am. J. Orthod. 85, 333, 1984). This investigation was designed to evaluate the relevance of crystal growth on the enamel surface as an alternative to conventional acid etching in direct bonding of orthodontic brackets. Annexing Li2SO4, MgSO4, KeSO4 respectively in the solution with 25% polyacrylic and 0.3M sulfuric acids were employed to enhance the crystal growth. Human bicuspids were treated with various parameters as combinations of crystal growth and glass ionomer cement, crystal growth and orthodontic resin, acid etching and orthodontic resin for an inveatigative purpose. Crystal growth solution cintaining MgSO4 showed the highest shear bond strength(15.6MPa) wuthin the groups of bonding brackets with glass ionomer cement(P<0.01). Bonding with glass ionomer cement on the surface treated with crystal growth solution containing MgSO4 or K2SO4 was not different shear bond strength statistically from bonding with orthodontic resin on the acid-etched surface. It suggests that bonding brackets with glass ionomer cement on the surface treated with crystal growth solution containing MgSO4 or K2SO4 is a potential altermative to bonding with resin on the acid etched sufrace.
Adhesives* ; Bicuspid ; Crystallization* ; Dental Enamel* ; Glass ; Glass Ionomer Cements ; Humans ; Orthodontic Brackets* ; Sulfuric Acids

We identified Lacrymaria velutina of the Coprinaceae in Korea. The unusually large and sturdy fruiting body, fibrillose to fibrillose-scaly cap and stalk without a volva with an obscure superior hairy ring zone or hairy annulus, and blackish brown, warted spores distinguished this species from closely related Psathyrella species. An illustrated account of the microscopic traits is presented. Fruiting bodies with obtusely hemispherical caps, 2.5~6 cm, becoming convex with age; surface dry, densely fibrillose-scaly with split margin; stipe, 4.5~6 cm, equal, hollow, fibrillose, dry, whitish above the superior ring zone, light brown below; crowded gills, adnexed, dark black at maturity. Pileipellis typically cellular with the gill edge appearing white and beaded. Blackish brown basidiospores that discolor in concentrated sulfuric acid. Spores elliptical, warted, 9~11 x 6~8 microm, with prominent snout-like germpores. Cheilocystidia abundant, 57~68 x 19~25 microm, and narrowly elongated clavate, often clustered in threes or fours. Pleurocystidia rarely present, 45~47.5 x 12~13 microm, and clavate to utriform. This trait distinguishes our sample as L. velutina from other Psathyrella spp. of the Coprinaceae, which have smooth spores. This taxon was clarified by the observation that Psathyrella spores fade in concentrated sulfuric acid. A molecular phylogenetic study revealed that our specimen was Lacrymria velutipes, which is closely related to Lacrymaria lacrymabunda. Moreover, those two species are clearly distinguishable from other Psathyrella species, which agreed with the morphologically distinctive traits described above. We believe that this is the first report of this taxon, which has not been described in Korea.
Animals ; Fruit ; Gills ; Humans ; Korea ; Light ; Spores ; Sulfur ; Sulfuric Acids ; Warts

OBJECTIVETo establish a method for determination of a variety of acid gas in the workplace air by Ion Chromatography. (hydrofluoric acid, hydrogen chloride or hydrochloric acid, sulfur anhydride or sulfuric acid, phosphoric acid, oxalic acid).

METHODThe sample in workplace air was collected by the porous glass plate absorption tube containing 5 ml leacheate. (Sulfuric acid fog, phosphoric acid aerosol microporous membrane after collection, eluted with 5 ml of eluent.) To separated by AS14+AG14 chromatography column, by carbonate (2.0+1.0) mmol/L (Na(2)CO(3)-NaHCO(3)) as eluent, flow rate of 1 ml/min, then analyzed by electrical conductivity detector. The retain time was used for qualitative and the peak area was used for quantitation.

RESULTSThe each ion of a variety of acid gas in the air of workplace were excellent in carbonate eluent separation. The linear range of working curve of 0∼20 mg/L. The correlation coefficient r>0.999; lower detection limit of 3.6∼115 µg/L; quantitative limit of 0.012∼0.53 mg/L; acquisition of 15L air were measured, the minimum detection concentration is 0.004 0∼0.13 mg/m(3). The recovery rate is 99.7%∼101.1%. In the sample without mutual interference ions. Samples stored at room temperature for 7 days.

CONCLUSIONThe same analysis method, the detection of various acidic gases in the air of workplace, simple operation, good separation effect, high sensitivity, high detection efficiency, easy popularization and application.

Acids, Noncarboxylic ; analysis ; Air ; analysis ; Air Pollutants, Occupational ; analysis ; Chromatography ; methods ; Gases ; Hydrochloric Acid ; Hydrofluoric Acid ; Ions ; analysis ; Limit of Detection ; Phosphoric Acids ; Sulfuric Acids ; Workplace

Acute Lung Injury ; etiology ; surgery ; Burns, Inhalation ; surgery ; Humans ; Lung Transplantation ; Male ; Middle Aged ; Respiratory Aspiration ; complications ; Sulfuric Acids ; poisoning

OBJECTIVETo study the reason for the deep dormancy of the aged Cuscuta chinensis seed and find the solving method.

METHODThe separated and combined treatments were applied in the orthogonal designed experiments.

RESULTThe aged seed had well water-absorbency; the water and ethanol extracts of the seeds showed an inhibition effect on germination capacity of the seeds.

CONCLUSIONThe main reason for the deep dormancy of aged C. chinensis seed is the inhibitors existed in seed. There are two methods to solve the problem. The seeds is immersed in 98% of H2SO4 for 2 min followed by 500 mg x L(-1) of GA3 treatment for 60 min, or in 100 mg x L(-1) of NaOH for 20 min followed by 500 mg x L(-1) of GA3 treatment for 120 min.

Cuscuta ; drug effects ; physiology ; Germination ; drug effects ; Gibberellins ; pharmacology ; Seeds ; drug effects ; physiology ; Sodium Hydroxide ; pharmacology ; Sulfuric Acids ; pharmacology

Patients with corrosive esophageal strictures have a high risk of developing carcinoma of the esophagus. For this reason, it is often recommended that resection of the esophagus should be considered for chronic caustic strictures in order to prevent the development of carcinoma. A 49-year-old woman who had undergone an esophagogastrectomy due to esophageal caustic stricture at the age of 23 was admitted with a 2-month history of dysphagia. Esophagoscopy showed a benign postoperative stricture in the remnant esophagus. After endoscopic balloon dilation of the stricture, which was about 5 cm in length, an ulcerative mass was noted distal to the stricture. This mass was diagnosed as squamous cell carcionoma. Nearly all the patients with corrosive carcinomas in the published literature had consumed an alkali drink, but our patient had a history of sulfuric acid ingestion.
Alkalies ; Constriction, Pathologic ; Deglutition Disorders ; Eating ; Esophageal Stenosis ; Esophagoscopy ; Esophagus ; Female ; Humans ; Middle Aged ; Sulfur ; Sulfuric Acids ; Ulcer