1.Karyotypes analysis of Ferula fukanensis.
Xin ZHAO ; Xiao-Jun MA ; Sulaiman KAISAR ; Chang-Liang FU ; Rui-Yang CHEN
China Journal of Chinese Materia Medica 2006;31(2):114-116
OBJECTIVEStudy on the karyotypes analysis of Ferula fukanensis.
METHODThe young roots were treated with 0.000 2 mol x L(1) 8-Hydroxyquinoline for 3 h, carnoy's for 3 h, 1 mol x L(-1) HCl in 5 min,carbol fuchsin coloration for 2 min and the treated roots were utilizied to make the plate for observation.
RESULTThe fukanensis is diploid. The chromosome number of somatic cells was 2n = 22. The karyotype formula is 2n = 2x = 20 = 16m + 4sm. The 4th and 10th are submetacentric, and the others are metacentric.
CONCLUSIONThe karyotype of F. fukanensis belongs to "2A" type of stebbins', and it is a primitive species.
Centromere ; Chromosomes, Plant ; genetics ; Diploidy ; Ferula ; genetics ; Karyotyping ; Plant Roots ; genetics ; Plants, Medicinal ; genetics ; Seeds ; genetics