Anti-rat lymphocyte rabbit serum was prepared with lymphoid tissue of rat spleen. Prednisolone, Imuran and this antiserum were used to prolong the survival of full-thickness sliin homograft in albino rats. The results were as follows. l. Administratio of tle anti-rat lymphocyte rabbit serum in a dose of 0. 3cc for 2 days before grafting and 0. 3cc every 3 days until rejection resulted in prolongation of the, urvival of the grafts. (Mean, 15.3 days) 2. Administration of preInisolone to the recipients in a dose of 2mg/kg/day from 7 days Iielore grafting to the time of rejection resulted in prolongation of survival of the, rafts. (Mean, 8.4 days) 3. Adminitration of Imuran to the reciipients in a dose of 5mg/kg/day from 7 day defore gvafting to the time cif rejection resulted in prolongation of the survival of ther afts. (Mean,14.6 days)
Allografts* ; Animals ; Azathioprine ; Immunosuppressive Agents* ; Lymphocytes ; Lymphoid Tissue ; Prednisolone ; Rats* ; Skin* ; Spleen ; Transplants

No abstract available.
Calcium* ; Homeostasis* ; Phosphorus*

No abstract available.
Cytogenetics* ; Diagnosis*

No abstract available.
Graft vs Host Disease*

Multiple symmetric lipomatosis is a rare disease and affects almost exclusively middle aged man, usually with a background of excessive a alcohol intake. The disease is characterized by progressive growth of subcutaneous fat masses which are located symmetrically at neck, shoulders, chest, abdomen and groin, and which subsequently penetrate deeply into the surrounding spaces and structures with symptomatic compression of deep organs, such as trachea. A recent survey revealed a high incidence of sometic and autonomic neuropathy. The exact cause of the disease is not known, but a hyperplastic mechanism has been postulated, with in vitro studies demonstrating a defect in adrenergic-stimulated lipolysis of lipomatous tissue. We have experienced two cases of multiple symmetric lipomatosis. Case 1 was a 59-year-old male, complaining of slowly enlarging doughunt ring-shaped mass at his neck. He had a habit of excessive alcohol intake for many years. The subcutaneous mass at the neck was excised. The pathology report described the specimen as "normal adipose tissue". Case 2 was a 49-year-old male, complanining of slowly enlarging multiple symmetric masses at the neck, shoulders, chest, abdomen, flank and groin over a period of 6 years. He also complained of mild muscular weakness. He had a habit of excessive alcohol intake for many years. The subcutaneous mass in the neck was excised. The specimen had a tendency to form globular masses and microscopically indistinguishable from mature adipose tissue.
Incidence

One hundred and four biopsy specimens of various dermatoses were stained with Periodic Acid-Schiff and examined. The lesions were grouped into five groups; A. twenty two cases of vesicob-ullous diseases; B. twenty one cases of maculopapulosquamous diseases; C. eighteen cases of chronic granulomatous infection; D. seventeen cases of precancerosis and benign and malignant neoplasms; and E. twenty six cases of miscellaneous skin diseases. Special attention was given to the morph-ological changes of the dermoepidermal junction and the basement membrane. The results were as follows. 1. Among 10 cases of erythema multiforme, partial fraying appeared in 4 cases, partial thicken-ing in 4 and hyperchromasia in 4. Marked convolution was noted in the thickened basement membrane. PAS-positive material was scattered in the edemateus papillary dermis in some cases. 2. All of 6 cases of pemphigus erythematosus and pemphigus vulgaris showed normal basement membrane. 3, In bullous pemphigoid, partial absence in the areas of bulla formation appeared in 2 cases am-ong 3 cases. PAS positive material was present within the bullae and in the upper dermis. 4. All of 3 cases of dermatitis herpetiformis showed partial absence or fraying, 5. Half of 10 cases of psoriasis showed normal basement membrane. Among the remainder, fraying and partial thickening appeared in 3 and 2 cases respectively. 6. Among 6 cases of lichen planus, almsot total absence appeared in 2 cases and fraying in 3 cases. PAS positive material appeared focally in the upper dermis in some cases. 7. Four of 5 cases of pityriasis rubra pilaris showed diffuse thickening and marked convolution ef the basement membrane. 8. Half of 4 cases of lupus vulgaris showed normal basement membrane. Fraying and partial thickening appeared in 2 and 2 cases respectively. 9. All but 1 among 5 cases of lepromatous leprosy showed poor staining and poor visualization of the basement membrane. 10. One case of tuberculoid leprosy showed partial fraying, partial thickening and hyperchromasia with marked convolution, among 5 cases. il. Among 4 cases of condyloma latum, only one showed partial fraying. 12. One of 3 cases of senile keratosis showed partial fraying of the basemement membrane. 13. One case of Bowens disease showed partial thickening and hyperchromasia among 3 cases. PAS-positive material was present focally in the upper dermis in some cases. 14. All of 6 cases of basal cell carcinoma, showed normal basement membrane. PAS-positive ma-terial were present focally in the uppermoet dermis in some vases. 15. All of 3 cases of squamous cell carcinoma showed total absence of the basement membrane. 16. All of the 2 cases of keratoacanthoma showed partial fraying. 17. Among 10 cases of chronic discoid lupus erythematosus, 9 cases showed partial thickening and hyperchromasia with marked convolution of the basement membrane. Two cases showed partial fraying in areas of marked liquefaction degeneration of the basal cells. PAS positive material appe-ared in the uppermost dermis in some cases. 18. All of 3 cases of fixed drug eruption, 2 cases of seborrheic keratosis and 6 cases of verrucae showed normal basement membrane. 19. One case of poikiloderma atrophicans vasculare showed partial fraying, among 2 cases. 20. All of 2 cases of chronic radiodermatitis showed diffuse thickening and hyperchramasia. The thickened basement membrane showed marked convolution focally.
Basement Membrane* ; Biopsy ; Bowen's Disease ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Dermatitis Herpetiformis ; Dermis ; Drug Eruptions ; Erythema Multiforme ; Keratoacanthoma ; Keratosis ; Keratosis, Seborrheic ; Leprosy, Lepromatous ; Leprosy, Tuberculoid ; Lichen Planus ; Lupus Erythematosus, Discoid ; Lupus Vulgaris ; Membranes ; Pemphigoid, Bullous ; Pemphigus ; Pityriasis Rubra Pilaris ; Psoriasis ; Radiodermatitis ; Skin Diseases ; Warts

The purpose of this study was to survey bacteria which was known as a fecal bacteria living in the human and animal gastro and intestinal tract. The colony density of the fecal coliform and fecal streptococci was 280 and 130 in 100ml sample amounts respectively. Staphylococcus aureus and Pseudomonas aeruginosa which is usually exist in swimming pool and was known as indicator of the pool water contamination was detected.
Animals ; Bacteria* ; Humans ; Pseudomonas aeruginosa ; Rivers* ; Staphylococcus aureus ; Swimming Pools

No abstract available.
Angiomyoma*

Many investigators have attempted to elucidate the basic pathogenic mechanisms of atopic dermatitis through clinical and laboratory investigations and have no longer attached to the etiological significance to IgE which is known to be a mediator of the so-called atopic diseases. In recent years, many authors have reported some abnormalities not onIy in the humoral but also in cellular immune status of patients with atopic dermatitis. although such findings are more or less controversial. (countinued..)
Dermatitis* ; Dermatitis, Atopic ; Humans ; Immunoglobulin E ; Research Personnel

Subepiderral calcified nodules is a special form of idiopathic calcinosis circumscripta with its distinct clinical and histopathologic features, which was originally described by Winer(1952) as solitary congenital no3ular czlcification of the skin. Although the lesions are usually solitary and congenital, subs aquent investigators reported multiple lesions and later onset. Clinically, th. leions are small hard. yellowish-white warty nodules developed. usually on the face and mostly in children and histopathologically are subepidermal calcified mass with s cvn4ry epidermal changes. Although the pathogenesis is still unexplained, it s seems that the disease is not associated with any systemic or cutaneous disease or any biochemial abnormalities. We present a first recorded case of subepidermal calcified no3ules developed on the left the a Korasn female, aged 23, which has been present for 7 years. Shave biopsy showed typical histopathologic and histochemical features of the subpidermal calcified nodules. Literature were reviewed.
Biopsy ; Calcinosis ; Child ; Female ; Humans ; Research Personnel ; Skin