Objective To investigate the regulative effect of cyclooxygenase-2 (COX-2) 3'-UTR in HT29 colon cancer cells. Methods Total RNA was extracted from HT29 colon cancer cells and used as a template to amplify COX-2 gene by reverse transcription polymerase chain reaction. Using PCR technique to construct a series of luciferase reporter gene expression vectors containing various regions of the 3'-UTR of COX-2 (including the whole gene region, gene region between 1 to 156 bp, gene region between 1 to 347 bp, gene region between 1 to 1006 bp, gene region between 156 to 347 bp and gene region between 157 to 2217 bp). These reporter gene expression vectors and pRL-SV40 were co-transfected in HT29 colon cancer cells by Lipofectamine 2000. The relative luciferase activity of the HT29 colon cancer cells was tested. All data were analyzed via t test. Results The luciferase activity was reduced by 70.4%, 37.4%, 64.8% and 24.2% in HT29 colon cancer cells transfected with the whole COX-2 gene region, gene region between 1 to 156 bp, gene region between 1 to 347 bp and gene region between 156 to 347 bp, respectively (t = 6.13, 7.73, 9.75, 3.92, P < 0.05). No obvious changes of luciferase activity were observed in HT29 colon cancer cells transfected with gene regions between 1 to 1006 bp and between 157 to 2217 bp (t = 0.13, 0.01, P > 0.05). Conclusions A region between 156-347 bp in the 3'-UTR of COX-2 has been found which can down-regulate the expression of COX-2 with the cooperation of the ARE element. The 3'-UTR of COX-2 contains several control elements that regulate the expression of COX-2 in colon cancer cells.

Objective To explore the related risk factors of stroke in progression(SIP)and establish discriminant models to predict the incidence.Methods 31 possible related factors were compared between progressive group(n=110)and non-progressive group(n=336),and the related factors were analyzed in discriminant models.Results Discriminant models were established by 16 factors,including arterial stenosis,OCSP subtype,blood glucose,low density lipoprotion,creatinine,the sign of early cerebral infarction on CT,the temperature increasing and the rapidly decreasing of blood pressure in 48 hours after admittion.The evaluation to the models by interview data and prospective data showed a satisfied result.Conclusion Arterial stenosis,OCSP subtype and other significant factors selected by model will be helpful in prediction of SIP.

Objective To analyze the changes of P wave of intracavitary electrocardiogram (ECC) in the placement of periph erally inserted central venous catheters (PICC), and to explore whether the intracavitary ECG can be used to guide the procedure. Methods PICC was inserted in 62 adult patients under intracavitary ECC-guided technique. The intracavitary ECC were recorded in different parts of vessel access through micro-guidewire. Results The intracavitary ECG extracted through micro-guidewire were accurately recorded in all patients. The amplitudes of P wave were (1.11 ±0.36)mm, (1.12±0.28)mm,(1.56±0.45)mm, (4.03±1.87)mm, (5.90±2.45)mm, (7.90±2.95)mm, (9.87±2.77)mm, (5.00±1.76)mm, (2.50±1.73)mm when PICC tip was located in the axillary, subclavian and brachiocephalic vein, in the superior, middle and inferior SVC, at the SVC-right atrium junction, and in the middle, inferior right atrium,respectively. The amplitude of P wave was 1.14±0.34mm in 10 patients with PICC tip malposition in the internal jugular vein. Conclusions The intracavitary ECG can be extracted with the microguidewire of PICC system. The amplitude of P wave is related to the location of PICC tip, which can be used to guide the PICC insertion and positioning.

Objective To investigate the effectiveness of background music in operating room on psychology of patients and medical personnels. Methods A total of 127 patients who would receive selective operation and 65 surgical staff in our hospital were randomly divided into the intervention group and the control group. There were 63 patients and 30 medical personnel in the intervention group, 64 patients and 35 medical personnel in the control group. Routine care was given to all patients, and background music was broadcasted after patients and medical personnels coming into operating room in the intervention group. The blood pressure and heart rate of patients were contrasted, and the anxiety status was evaluated by Self-Rating Anxiety Scales (SAS), Visual Analogue Scale was used to appraise the horror, discomfort and pain between the control group and the intervention group. Results Diastolic pressure and systolic pressure was higher and heart rate was more rapid in the control group than in the intervention group. SAS score of patients and medical personnel was higher in the control group than in the intervention groups, degree of horror, pain and complaint in patients was more apparent in the control group than in the intervention group. Conclusions Background music could release negative emotion and was helpful for stabling the mood of medical personnel and preserving physical and mental health of patients.

Objective To compare the effectiveness and safety of central venous catheters inserted from the left side and right side during peripheral inserted central catheterizations (PICC). Methods Totally 458 adult patients undergoing PICC between May 2007 and May 2008 were enrolled in this study and divided randomly into right-sided group (n = 228)and left-slded group (n = 230). Chest X-ray was performed immediately after catheterization to identify the initial tip locations. Other parameters were evaluated during follow-up. Results The rate of difficult insertion was significantly lower in right-sided group than in left-sided group (14.9% vs 24. 8% , P =0.003). The rate of tip projection angle ＞40°was also significantly lower in right-sided group (2.2% vs 23.4% ,P = 0. 000). The rate of tips reaching the central veins was not significantly different between two groups (54.4%vs 53.0% , P = 0. 538). Compared with right-sided catheters, the tip positions in the left-sided group was significantly less frequently located in the inferior segment of superior vena cave in the central tip locations (6. 6% vs 21.0% , P =0. 001)and more commonly positioned in the nominate vein in non-central tip locations (66. 7% vs 48.1% , P = 0. 008). In addition, the catheter detaining time (P = 0. 617), incidence of local phlebitis after puncture (P = 0. 561), catheter obstruction rate (P = 0. 774), and catheter-related infection rate (P = 0. 854)showed no significant differences between two groups. The incidence of swollen limb was significantly lower inright-sided group than in left-sided group (4. 4% vs 8.3%, P = 0. 043). Conclusions Right-sided catheters provide better outcomes than left-sided catheters. PICC through the right elbow veins should be preferred in clinical practices.

This study was aimed to explore effects of Jinzhen Koufuye (JZKFY) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Rats were randomly divided into the normal group, model group, positive control group, and three JZKFY groups with the drug dosage of 4.4, 2.2, 1.1 g·kg-1, respectively. Intragastric administration was given to animals for 7 consecutive days. One hour after the last administration, ALI model was induced by the injection of LPS (6 mg·kg-1), and then rats were killed at 16 h after saline (control) or LPS injection. Histological examinations were performed on the lungs. At the same time, the lung permeability index, activities of MPO, MDA and SOD in lung tissues, contents of TNF-α, IL-6 and IL-1β in serum were measured, respectively. The results showed that JZKFY of high-, medium-, or low-dosage can significantly reduce JZKFY lung permeability index. JZKFY of high- and medium-dosage can obviously regulate activities of MPO, MDA and SOD in lung tissues, re-duce contents of TNF-α, IL-6 and IL-1β in serum, as well as significantly improve lung tissue lesions in ALI. It was concluded that JZKFY might improve ALI induced by LPS through inhibiting lung vascular permeability, reduc-ing the lung neutrophil aggregation, improving the ability of antioxidative stress and lowering inflammatory response.

Objective To investigate the effect ofpeptidoglycan from Staphylococcus aureus on the release of several chemokines including intedeukin 8 (IL-8), regulated upon activation, normal T cell expressed and secreted (RANTES), macrophage-derived chemokine (MDC) by normal human epidermal keratinocytes (KCs) and the role of Toll-like receptor 2 (TLR2) in this process. Methods KCs were derived from the foreskin of a healthy boy and propagated. After 2 - 4 passages, KCs were collected and treated with various concentrations (3, 10, 30 and 100 mg/L) of peptidoglycan for 24 hours or with peptidoglycan of 100 mg/L for varying durations (3, 6, 12, 36 hours). A fi'action of KCs were pretreated with functional grade purified anti-TLR2 monoclonal antibody before the treatment with peptidoglycan of 100 mg/L. After additional 12-hour culture following the treatment, enzyme linked immunosorbent assay was used to detect the level of IL-8, RANTES and MDC in culture supernatants of KCs. Results KCs spontaneously released IL-8 and RANTES. Peptidoglycan increased the production of IL-8 but decreased that of RANTES by KCs. The levels of IL-8 were 209.96 ± 10.31 ng/L, 250.28 ± 9.52 ng/L, 285.11 ± 10.28 ng/L, 359.40 ± 6.93 ng/L in KCs treated with peptidoglycan of 3, 10, 30, 100 mg/L, respectively, compared to 135.41 ± 14.37 ng/L in untreated KCs (all P < 0.05). On the contrary, a significant decrement was seen in the secretion of RANTES by KCs treated with peptidoglycan of 10, 30, 100 mg/L compared with untreated KCs (110.72 ± 8.51 ng/L, 90.50 ±2.45 ng/L, 49.89 ± 13.74 ng/L vs 149.94 ± 18.71 ng/L, all P < 0.05). The monoclonal antibody to TLR-2 could markedly suppress the promotion of IL-8 production by peptidoglycan, but had no obvious influence on the inhibition of RANTES production by peptidoglycan. MDC could not be detected in the culture super-natants of KCs with or without peptidoglycan stimulation. Conclusion Peptidoglycan could inhibit RANTES secretion but induce IL-8 production by KCs likely via TLR2.

Objective To explore the relationship between tongue conditions and hemorrheology in acute stage of cerebral hemorrhage and to increase the accuracy of tongue conditions for the syndrome differentiation of acute stage of cerebral hemorrhage.Methods Eighty-eight qualified patients were enrolled into this study.The tongue conditions(tongue color,tongue coating and tongue shape) and hemorrheological indexes(whole blood viscosity,whole blood reduced viscosity,plasma viscosity,hematocrit and fibrinogen) were examined,and their relationship was also analyzed.Results The changes of tongue shape was obvious and low-shear whole blood viscosity was increased in the swollen tongue group(P

The object of using parasitic germplasm resource is to raise the technical level to control parsitosis for domestic animal and poultry.A total of 1 036 parasitic species collected from 16 kinds of domestic animals and poultries were reported in South-western China,where is one of the most serious areas for parasitosis transmission in China.This paper described the research and use of the new techniques on diagnosis,surveillance and control of parasitosis in domestic animal and poultry in Sichuan and South-western China,especially on applying resources of parasitic species in the pilot areas,so that the capacity of control and prevent of parasitosis in sheep,pig and dairy cattle farms were reasonably improved.