Objective: To observe the biocompatibility of titanium implant surfaces using laser scanning confocal microscopy (LSCM). Methods; Titanium implant surfaces were prepared by 3 different methods. Osteoblasts were seeded onto the titanium surfaces. The cells were investigated by LSCM. Results: The sand-blasted titanium surfaces showed the best biocompatibility. Conclusion; The biocompatibility of titanium implant surfaces depends on the surface preparation method.

Hepatitis C, Chronic ; Humans

Objective To know the incidence of pollen allergy among Beijing residents over the age of 15. Methods In 2005-2006,10 966 people aged 15-75 years were randomly selected and the information on pollen allergy and pollinosis was investigated by the telephone questionary. Results The response rate of the telephone survey was 67.2%,the total incidence of pollinosis was 2.51%,the incidence for male and female was 1.63% and 3.73% respectively,the difference was statistically significant(P

OBJECTIVE To discuss the constitution and drug sensitivity of pathogens that cause pulmonary infection in NICU. METHODS Sputa were collected from patients hospitalized in NICU from Mar 2006 to Mar 2007 and analyzed for distribution and drug sensitivity of pathogenic bacteria. RESULTS All 555 strains of pathogenic bacteria were isolated. The most common pathogens were Pseudomonas aeruginosa(183 strains,33.0%),Staphylococcus aureus(114 strains,20.5%) and Klebsiella pneumoniae(56 strains,10.1%). Nearly most pathogenic bacteria were multi-resistant to commonly used antibiotics. Meticillin resistant strains of S. aureus accounted for 97.4%. The percentange of fungi strains was increasing in NICU. The main isolated strains were Candida tropicalis(5.9%),C. albicans(3.8%)and C. glabrata(3.6%). And all of the fungi were sensitive to amphotericin B. fluconazole. and ketoconazole. CONCLUSIONS Pulmonary infections in NICU are mainly caused by Gram-negative bacteria with high rate of drug resistance. It can be of great importance to make drug sensitivity tests at regular intervals to guide the use of antibiotics.

Objective To investigate the clinical effect of mosapride combined acupoint massage on diabetic gastroparesis(DGP)in elderly patients with type 2 diabetes.Methods 150 cases of DGP in our hospital from April 2015 to May 2016 were collected and randomly divided into three groups:the conventional treatment group(n=35,in insulin treatment),the control group(n=52,in mosapride treatment),and the experimental group(n=63,in mosapride combined with acupoint massage treatment).All patients received the conventional diabetic therapy.Clinical efficacy,gastric emptying rate and the levels of serum gastrin and fasting blood glucose were observed and compared before versus after treatment among the three groups.Results After treatment,the total effective rate was 92.1% in the experimental group,versus 51.4% and 73.1% in the conventional treatment group and control group respectively,showing a statistically significant difference(P<0.05).There were significant differences in gastric emptying rate,the levels of serum gastrin and fasting blood glucose among the 3 treatment groups before versus after treatment.And gastric emptying rate,the levels of serum gastrin and fasting blood glucose were improved in the experimental groups compared with the conventional treatment and control groups(all P<0.05).The follow-up results showed that the adverse reactions and the recurrence rates were significantly lower in the experimental group than in conventional treatment group and control group(all P<0.05).Conclusions Mosapride combined with acupoint massage in the treatment of diabetic gastroparesis have significant clinical effect,low recurrence rate and high safety,and is worthy of promotion.

Objective To explore the diagnostic value of impulse oscillometry(IOS) in the patients with COPD. Methods The IOS parameters of 142 cases of COPD (COPD group) were measured and compared with those of 160 normal eases (control group). Linear correlation analysis was done between the IOS parameters and the conventional pulmonary function indexes such as FEV1 / Pred and FEV1/FVC, and the diagnostic value of the IOS parameters in COPD was discussed. Results Compared with those of the control group, respiratory impedance (Zrs),resistance at 5 Hz and 20 Hz (R5,R<<20>) and their difference (R5-R20), central resistance (Re) and peripheral resistance (Rp), resonance frequency (Fres)in the COPD group increased significantly, while reactance at 5 Hz (X5) decreased obviously (P< 0.01 ). In the COPD group, Zrs, Fres, R5, R20, R5-R20 and Rp were all correlated negatively with FEV1 / Pred, but X5 was correlated positively with FEV1/Pred. And the most strong correlation was found between Fres and FEV1/Pred. Fres, R5, R5-R20, Rp were correlated negatively with FEV1/FVC( P all < 0.05). Conclusions IOS is suitable for the diagnosis of COPD,and can be used to evaluate the severity of COPD. The application perspective is very extensive.

BACKGROUND: The rehabilitative intervention accelerates the recombination and reconstruction of cerebral structure and function and then promotes the amelioration of function.OBJECTIVE: To evaluate the influence of early and late rehabilitative interventions on the motor function and activities of daily living (ADL) with neurologic deficit score (NDS), Fugl-Meyer assessment (FMA) and modified Barthel index in patients with cerebral infarction.DESIGN: A randomized controlled trial.SETTING: Department of Neurology, Qilu Hospital of Shandong University; Department of Rehabilitation, Jinan Great Wall Hospital; Department of Neurology, the Third People' s Hospital of Heze.PARTICIPANTS: Totally 216 inpatients with cerebral infarction (125 males and 91 females, aged 60-75 years), who were selected from Qilu Hospital of Shandong University, Jinan Great Wall Hospital and the Third People's Hospital of Heze from December 2000 to December 2003, were randomly divided into early rehabilitation group (n=108) and late rehabilitation group (n=108) after admission.INTERVENTIONS: In the early rehabilitation group, the patients began to receive rehabilitation at 48 hours to 14 days after the stability of vital signs and absence of the progress of neurological signs. In the late rehabilitation group, the patients began to receive rehabilitation at 15-30 days after attack. They were trained with Bobath method and motor relearning program, once a day, 45 minutes for each time, and 6 times every week.Before and 30 days after the rehabilitative treatment, the rehabilitation was evaluated with modified Barthel index (100 points as normal, 0-20 as extremely severe functional defect, 25-45 as severe functional defect, 50 -70 as moderate functional defect, 75-95 as mild functional defect), FMA (total score was 100 points, including the highest scores of upper and lower limb movement were 66 and 34 points respectively) and NDS (the highest and lowest scores were 45 and 0 point, 0-15 as mild, 16-30 as moderate, 31-45as severe).ter treatment.RESULTS: All the 216 patients with cerebral infarction were involved in obviously lower than that before treatment in both groups (P ＜ 0.01), lower in the early rehabilitation group than in the late rehabilitation group score at 30 days after treatment was obviously higher than that before treat ment in both groups (P ＜ 0.01), higher in the early rehabilitation group than in the late rehabilitation group [upper limb: (32.43±21.52), (26.69±19.79)dex: The modified Barthel index at 30 days after treatment was obviously higher than that before treatment in both groups (P ＜ 0.01), higher in the early rehabilitation group than in the late rehabilitation group [(54.23±30.33),(46.57±29.85) points, P ＜ 0.05].CONCLUSION: Both early and late rehabilitative interventions can obviously accelerate the recovery of neurological function, motor function and ADL, but the effect of early rehabilitative intervention is superior to that of the late one.

Objective To analyze the diversity of intestinal microecology in different healthy age groups and explore the relationship between age and the composition of intestinal microflora.MethodsFecal samples were collected from the children,adult and elderly groups.The main intestinal microflora were cultured and quantified,and the qualitative and quantitative changes in the level of category were determined by fluorescence quantitative PCR.The Bifidobacteria/Enterobacteriaceae (B/E) ratio reflexing intestinal colonization resistance was also calculated.Results The germiculture of fecal samples indicated that the counts of Bifidobacteria and Lactobacillus (P＜0.01 and P＜0.05,respectively) were significantly lower in the adult and aged groups than in children group.The number of Bacteriodes and Enterobacteriaceae in the elderly group were higher than in the children and the adult groups (all P＜0.01).The counts of Enterococcus were increased in the adult and the aged groups (P＜0.05 and P＜0.01) compared with the children group,and were higher in the aged group than in the adult group (P＜0.05).The B/E ratio was significantly decreased in the aged group (0.88-±-0.13) as compared with the children (1.15±0.15) and the adult groups ( 1.01± 0.20) ( both P ＜ 0.01).The diversities of Bifidobacteria,Bacteriodes,Enterobacteriaceae and B/E ratio by fluorescent quantification PCR were consistent with the results by germiculture technology.The diversity of Lactobacillus (P＜0.01) between the children and the aged groups as well as between the children group and the adult group significantly improved (P＜0.01 and P＜0.05 ) in PCR compared with the germiculture technology. ConclusionsThe numbers of intestinal Bifidobacterium and Lactobacillus are decreased with lower colonization resistance,while the numbers of Bacteriodes,Enterobacteriaceae and Enterococcus are increased with aging.

Objective To compare the effectiveness and safety of central venous catheters inserted from the left side and right side during peripheral inserted central catheterizations (PICC). Methods Totally 458 adult patients undergoing PICC between May 2007 and May 2008 were enrolled in this study and divided randomly into right-sided group (n = 228)and left-slded group (n = 230). Chest X-ray was performed immediately after catheterization to identify the initial tip locations. Other parameters were evaluated during follow-up. Results The rate of difficult insertion was significantly lower in right-sided group than in left-sided group (14.9% vs 24. 8% , P =0.003). The rate of tip projection angle ＞40°was also significantly lower in right-sided group (2.2% vs 23.4% ,P = 0. 000). The rate of tips reaching the central veins was not significantly different between two groups (54.4%vs 53.0% , P = 0. 538). Compared with right-sided catheters, the tip positions in the left-sided group was significantly less frequently located in the inferior segment of superior vena cave in the central tip locations (6. 6% vs 21.0% , P =0. 001)and more commonly positioned in the nominate vein in non-central tip locations (66. 7% vs 48.1% , P = 0. 008). In addition, the catheter detaining time (P = 0. 617), incidence of local phlebitis after puncture (P = 0. 561), catheter obstruction rate (P = 0. 774), and catheter-related infection rate (P = 0. 854)showed no significant differences between two groups. The incidence of swollen limb was significantly lower inright-sided group than in left-sided group (4. 4% vs 8.3%, P = 0. 043). Conclusions Right-sided catheters provide better outcomes than left-sided catheters. PICC through the right elbow veins should be preferred in clinical practices.

Objective To investigate the status and trends of prostate cancer mortality in China.Methods Prostate cancer data between 1998 and 2008 from the National Cancer Registration Center were collected,including the mortality of prostate cancer,the cumulative probability of dying from prostate cancer of males aged 0-74,composition and age-specific mortality.Comparing and analysing the status and trends of the mortality between urban and rural men.Results In 2008,the mortality of prostate cancer of chinese men was 4.07/100 000,the world's population age-standardized mortality rate was 2.44/100 000.The mortality cumulative risk of males aged 0-74 was 0.18％,accounting for 1.78％ of the mortality of men's malignant tumors.The mortality of urban males was about 2.7 times of that in rural areas.The results of age-specific mortality showed that the mortality of chinese males over the age of 70 ranked lth in the males genitourinary tumor.The average annual growth rate during 1998-2008 was 8.44％.The growth mortality rate of urban men with prostate cancer was higher than in rural areas,it was 2.49/100 000 and 0.96/100 000,but the average annual growth rate was lower than rural males,it was 7.86％ and 8.28％.The proportion of high age group was increased significantly in the age composition of the mortality over time.Conclusions In recent years,the mortality of prostate cancer has showed a obvious growth trend in China.Prostate cancer impacts Chinese men's health seriously.