Objective To analyze the discrepancies between position adjustments obtained with the stereoscopic 2DKV XGS-10 system and the Varian OBI system for head-and-neck region IMRT treatments,and to compare for image acquisition and registration time.Methods CBCT images were obtained with OBI system and 2DKV images were acquired by XGS-10 system for 30 head-and-neck patients prior to Varian21EX IMRT treatment.The images were registered with planning image for localization,and position adjustments were given in LR,SI and AP directions,then the discrepancies between them were analyzed.On the comparison of the two different systems,the Pearson coefficient was used to analyzed the correlation and 95％ CI analysis to discern the consistence.Results Analysis of images acquired for the 30 patients yielded the following results:position adjustments with XGS-10 system were (-1.03 ± 2.15) mm,(0.86 ± 2.59) mm,(0.42 ± 1.66) mm in LR,SI and AP directions,whereas (0.00 ± 1.68) mm,(1.53 ± 2.12) mm,(0.10 ± 1.54) mm with CBCT in LR,SI and AP directions.The discrepancies were (-1.03 ± 1.24) mm,(-0.68 ± 1.78) mm and (0.32±1.61) mm in LR,SI and AP directions.The correlation coefficients between them were 0.817,0.731 and 0.495 in LR,SI and AP directions.95％ CI were (-1.47--0.59),(-1.32-0.04),(-0.26-0.90) mm.The average image acquisition and registration time were 10 s and ＜ 15 s in XGS-10 system,with 3 min and 8 min in OBI system.Conclusions Both of XGS-10 system and OBI system could be used to improve patient position accuracy,but XGS-10 system could cut down the total time.

Objective To observe clinical effect of acupuncture combined with rehabilitation training on spastic cerebral palsy. Methods 160 patients with cerebral palsy were divided into acupuncture group(n=80) and control group(n=80). The acupuncture group received acupuncture, including the acupoints such as Baihui, Sishengcong, Yamen, Fengchi, etc., and combined with function training. The control group received Bobath appoach as rehabilitation training. Two groups were treated for 3 months. Results The acupuncture group(n=77) showed powerfully for 26 cases, effective for 46 cases, and invalid for 5 cases, and the efficient rate was 93.5%. The control group (n=78) showed powerfully for 20 cases, effective for 45 cases, invalid for 13 cases, and the efficient rate was 83.3% (P<0.05). Conclusion Acupuncture combined with rehabilitation training can facillitate to reduce muscle tone of spastic cerebral palsy, and improve effect of rehabilitation.

Secretion of the salivary glands of leeches contains more than 100 bioactive substances,in recent years it has become the focus of many researchers'attention, which was the most extensive and in-depth study of the salivary glands of leeches anticoagulant and inhibit the biological activity of platelet aggregation substance, and some species have been successfully applied to the treatment of cardiovascular diseases.in addition, a growing number of studies have found that leech salivary gland secretion also has antibacterial, antitumor, analgesic, vasodilator, anti-inflammatory, wound healing and other biological functions.Thus, leech salivary gland secretion with functions of biological diversity.Moreover, the leech is not just for the treatment of human diseases, but also applied to the treatment of several diseases of animals.This review expatiating the functional diversity of leech salivary secretions by consulting a large number of iterature , that the use of leech salivary gland secretion and other blood-sucking animal salivary gland secretions functions provide a useful reference.

OBJECTIVE:To explore the prognosis effect of Shengmai injection to the rt-PA thrombolysis on broadened thera-peutic window patients of mild-to-moderate cerebral infarction under the guidance of computer tomography(CT). METHODS:168 patients with mild-to-moderate cerebral infarction were randomly divided into control group (84 cases) and observation group (84 cases). Control group received rt-PA(0.9 mg/kg),and 10% of the total rt-PA was mixed and injected into patients within 1 min, the rest 90% adding into 250 ml 0.9% Sodium chloride injection (no less than 1 h),CT was performed after 24 h thrombolytic therapy,and the patients with no intracerebral hemorrhage in re-examination received 300 mg/d aspirin for 10 d,then 100 mg/d for 90 d,and appropriate statins,calcium antagonists,β-blockers and nitrates;observation group additionally received 40 ml Sheng-mai injection,adding into 250 ml 5% Glucose injection,intravenous infusion,once a day,for 28 d. National Institutes of Health Stroke Scale (NIHSS) score,Rankin score,daily living-Barthel Index (Barthel) score,rate of symptomatic intracranial hemor-rhage,rate of secondary hemorrhage,hospitalization time,3-month postoperative mortality and incidence of adverse reactions in 2 groups were observed. RESULTS:Before treatment,there were no significant differences in the NIHSS score,Rankin score and Barthel score(P>0.05). The NIHSS score in 2 groups after 6 h,24 h and 7 d were significantly lower than before,and observa-tion group was lower than control group after 24 h and 7 d;Rankin score after 3 months was significantly lower than before,and observation group was lower than control group;Barthel score after 3 months was significantly higher than before,and observation group was higher than control group,The differences were statistically significant (P<0.05). The hospitalization time in observa-tion group was significantly shorter than in control group,and 3-month postoperative mortality was significantly lower than control group,the differences were statistically significant(P<0.05). There were no obvious adverse reactions during treatment. CONCLU-SIONS:Based on conventional treatment,Shengmai injection has improvement in the rt-PA intravenous thrombolysis on broadened therapeutic window patients of mild-to-moderate cerebral infarction under the guidance of CT,it can reduce NIHSS score and Rankin score,increase Barthel score,shorten hospitalization time and reduce the 3-month postoperative mortality,with good safety.

Under the endeavor of several generations,department of pharmacology,school of pharmaceutical sciences,Central South University,now has become the national key discipline.It is in possession of the right to confer master and doctor degree and is a mobile postdoctoral station.In 2004,department of pharmacology gained the national exquisite course.In recent years,taking the chance of welcoming the teaching evaluation of the ministry of education,a series of teaching reform and investigation has been carried out which can both improve the standards of teaching and accelerate the stable progression of the discipline.

Objective To observe the effect of electroacupuncture on migraine. Methods 60 patients were divided into electroacupuncture group (30 cases) who received electroacupuncture, and control group (30 cases) who received local nerve block. Results In the electroacupuncture group, 22 (73.33%) cases were cured, 6 (20.00%) cases were obviously improved, 2 (6.67%) cases were improved. While in control group, 17 (56.67%) cases were cured, 5 (16.67%) cases were obviously improved, 8 (26.67%) cases were improved. There was significant difference between these two groups (P<0.05). The time of treatment was less in the electroacupuncture group than in the control (P<0.05). Conclusion Electroacupuncture is effective on migraine.

Objective To investigate the influencing factors for the short-term prognosis in elderly patients with posterior circulation infarction after intravenous thrombolysis.Methods We retrospectively analyzed the clinical data of 67 elderly patients with posterior circulation infarction treated with thrombolysis from March 2011 to December 2012.Clinical data of age,sex,hypertension,hyperlipidemia,glucose abnormalities,and thrombolysis,National Institute of Health stroke scale (NIHSS) score on admission,hyperhomocystinemia,the infarction area,anticoagulation and antiplatelet treatment were collected.All patients were followed up after discharge.The prognosis was assessed by the Rankin scale (mRS) after 3 months of thrombolysis.Patients were divided into good (MRS score ≤2) prognosis group and poor (mRS＞2) prognosis group.Results There were 43 cases (64.2％) in good prognosis group and 24 cases (35.8％) in poor prognosis group.Logistic regression analysis showed that NIHSS score＞12 (95％CI:1.087-1.569,P=0.005),thrombolysis time＞4.5 h (95％CI:1.362-2.258,P=0.004),high homocysteine levels (95％CI:0.612-0.956,P =0.000),platelet after 24 h (95％ CI:0.785 1.133,P=0.001) were the independent risk factors for the poor prognosis of posterior circulation infarction in elderly patients after intravenous thrombolysis.Conclusions Early thrombolysis and timely anticoagulant treatments have certain significances in improving the short-term prognosis in patients with posterior circulation infarction.

Objective To study the clinicopathological features of primary and metastatic hepatic neuroendocrine carcinoma.Methods The records of 35 patients with primary hepatic neuroendocrine carcinoma and 35 patients with metastatic hepatic neuroendocrine carcinoma were retrospectively reviewed.These patients served as the primary group(priNET,n=35)and the metastasis group (metNET,n=35),respectively.Results There were significant differences between the two groups of patients in gender,site,size and number of tumor(P＜0.05).Although there was no significant difference between the two groups in the distribution of the tumors in the two lobes of liver (P＞0.05),priNET had more tumors localized to one lobe of liver while metNET had more tumors involving both lobes of liver(P＜0.05).Conclusions Gender,size,site and number of tumor may play an important role in the differentiation of primary or metastatic hepatic neuroendocrine tumor.

Objective To establish a rat model of tympanosclerosis(TS) by myringotomy and inoculation of streptococcus pneummoniae, and to observe the morphological change in the tympanic membrane (TM) and middle ear mucous.Methods Forty Sprague-Dawley rats were randomly divided into two groups (n=20/group) and then ten in each group were chosen to serve as the control and the other ten were set up for the TS model.Group A (myringotomy): myringotomy was performed on the bilateral TMs of all rats except the control group.Group B (bacterial inoculation): streptococcus pneumoniae was inoculated into the bilateral middle ear cavity of all rats except the control group.The condition of the TMs and the middle ears in the two groups were respectively examined at 2 weeks after myringotomy and at the five time points (1 week, 2 weeks, 4 weeks, 6 weeks, and 8 weeks) after bacterial inoculation.Then the rats were decapitated and the morphological changes were observed by hematoxylin and eosin staining.Results One rat in group B died two weeks after the inoculation.In the two experimental groups, the calcifications were observed in 70%of the TMs (14/20) in group A and in 33.33%of the TMs (6/18) at 8 weeks in group B.At the same time, the inflammatory infiltration and hyaline degeneration markedly appeared in the tympanic membrane and middle ear mucous membrane.In the two control groups, neither morphological changes nor calcifications occurred.Conclusion The current study indicated that the animal model of TS was successfully accomplished by myringotomy and inoculation of streptococcus pneummoniae, and their morphological changes were basically consistent.However, the method of myringotomy is easier to use and can obtain a higher modeling rate in a relatively short time.