Objective To explore the effects of mindfulness-based stress reduction ( MBSR ) on anxiety, depression and immune function in patients with gastric cancer .Methods The study investigated 118 patients with gastric cancer who were hospitalized from March 2011 to February 2015 .The participants were randomly divided into the intervention group ( n=59 ) and the control group ( n=59 ) .Both groups received conventional mental health education while the intervention group complemented with MBSR intervention once a week ( six weeks in total ) .Self-rating anxiety scale ( SAS) and self-rating depression scale ( SDS) were used to evaluate patients′anxiety and depression status before and after the intervention .Moreover , T-cell subsets status could reflect the immune function using phosphatase bridging ELISA .Results Before the intervention , the scores of SAS and SDS had no significant differences between the two groups ( P>0.05).However, after the MBSR intervention, the scores for the intervention group significantly reduced and lower than the control group (P<0.05) since the scores for the control group had limited changes (P>0.05).Furthermore, the CD3+, CD4+, CD8 +and CD4+/CD8+had little differences between the two groups before the intervention ( P>0.05) . However , the CD3+, CD4+ and CD4+/CD8+ for the intervention group significantly increased with CD 8+reduced after MBSR (P<0.05), and the differences between the two groups were statistically significant after MBSR(P<0.05), but there were little changes in the control group ( P>0.05).Conclusions The MBSR intervention can significantly reduce the negative emotions such as anxiety and depression , and can increase the immunity in patients with gastric cancer .

ObjectiveThe study aimed to evaluate the measurement uncertainty in the determination of five cacaine anesthetics and their metabolites residues by high-performance liquid chromatography-tandem mass spectrometry （LC-MS/MS）. MethodsThrough the establishment of mathematical model， the sources of uncertainty were analyzed， and various components were quantified and synthesized to evaluate the influence of the uncertainty components on the measurement results． ResultsThe uncertainties of the experiments were mainly derived from calibration curve fitting， sample pretreatment， recovery rate， standard solution preparation and measurement repeatability. Furthermore， the expanded uncertainty related to the content in shrimp （k=2） was 1.18 μg‧kg^-1 at 8.68 μg‧kg^-1 for tricaine methanesulfonate （MS-222）， 1.28 μg‧kg^-1 at 9.11 μg‧kg^-1 for benzocaine， 13.5 μg‧kg^-1 at 91.4 μg‧kg^-1 for 4-aminobenzoic acid， 12.2 μg‧kg^-1 at 91.0 μg‧kg^-1 for p-acetylamino benzoic acid， and 11.3 μg‧kg^-1 at 95.3 μg‧kg^-1 for 3-aminobenzoic acid， respectively. ConclusionThis method is suitable for the uncertainty analysis of cacaine anesthetics and their metabolites determination in aquatic products by LC-MS/MS， and can provide scientific and reliable basis for the measurement accuracy.