Cytotoxic test in vitro combined with cytochemical stain, fluorescent stain, transmission electronmicrograph was used to study the vacuolated effect by helicobacter pylori (H.pylori) (Toxin＋) and its pathological mechanism. 78.26 % patients with peptic ulcer associated with H.pylori was infected with H.pylori (Toxin＋), while 42.86 % patients with gastritis was infected with H.pylori (Toxin＋). It was positive in vacuole with acridine orange and acid phosphatase stain. Transmission electronmicrograph of vacuole revealed the presence of abounding membrane. There was a closed relationship between infection with H.pylori (Toxin＋) and peptic ulcer disease. The vacuole induced by H.pylori (Toxin＋) was autophagosome, which was pathological phenomenon induced by toxin.

Cytotoxic test in vitro combined with cytochemical stain, fluorescent stain, transmission electronmicrograph was used to study the vacuolated effect by helicobacter pylori (H.pylori) (Toxin＋) and its pathological mechanism. 78.26 % patients with peptic ulcer associated with H.pylori was infected with H.pylori (Toxin＋), while 42.86 % patients with gastritis was infected with H.pylori (Toxin＋). It was positive in vacuole with acridine orange and acid phosphatase stain. Transmission electronmicrograph of vacuole revealed the presence of abounding membrane. There was a closed relationship between infection with H.pylori (Toxin＋) and peptic ulcer disease. The vacuole induced by H.pylori (Toxin＋) was autophagosome, which was pathological phenomenon induced by toxin.