Objective In this study,we investigated the hair cell apoptosis and the molecular mechanisms in age-related hearing IOSS mouse cochlea.Methods All animals were assessed by ABR and DPOAE analysis in both ears.TUNEL(Terminal dexynucleotidyl transferase(TdT)mediated dUTP Nick End Labeling)and PI were used to identify DNA fiagments and caspase-3 activities in hair cells.Results The results showed that the nmf308 mice had progressive hair cell loss along with age.The cochlear OHCs were reduced 5%～10% at 1 month and 100% at 3 month in the basal region.Substantial amounts of TUNEL-postive OHCs nuclei were observed at age of 1 month,and activated caspase-3 labeling OHCs were most obviously observed at age of 2 months.Conclusion These results suggest that DNA single strand break is attributed primarily to apoptosis of cochlear lesion,and activation of caspase-3 at leter stage leads to the hair cell apoptiovs of nuclear condensation in age-related hearing loss mouse cochlea.

OBJECTIVE To explore the advantage of exposure recurrent laryngeal nerve in thyroidectomy. METHODS Exposure the recurrent laryngeal nerve was performed deliberately in 452 cases during thyroidectomy. The larynx was examined with laryngoscope before and after operation. RESULTS Of the 452 cases with 748 exposed recurrent laryngeal nerve, only 14 cases (1.88%) occurred temporary recurrent laryngeal nerve palsy, and 2 cases (0.27%) occurred permanent recurrent laryngeal nerve palsy. CONCLUSION Exposure the recurrent laryngeal nerve during thyroidectomy can protect the nerve from surgery injury.

OBJECTIVE: To study the complications in total thyroidectomies and the safety of total thyroidectomy. METHOD: Retrospective analyses 351 cases underwent total thyroidectomy in our department. Preoperative and postoperative electronic laryngoscopy were used to assess the vocal cord function. All patients have serum calcium analysis on the operative day and 3rd and 7th day after operation. RESULT: Temporary hypocalcaemia occurred in 47.01% after total thyroidectomy, but only 15.67% with severe symptom temporarily,and 21.37% with temporary mild numbness. Permanent hypoparathyroidism occurred in 0.85% patients. None with bilateral recurrent laryngeal nerve palsy, and temporary unilateral vocal cord palsy occurred in 1.42% patients and permanent unilateral recurrent laryngeal nerve palsy occurred in 0.56% patients. CONCLUSION Total thyroidectomy is safe with low complications.
Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Safety ; Thyroid Neoplasms ; surgery ; Thyroidectomy ; adverse effects ; methods ; Young Adult

OBJECTIVE: To investigate the surgical treatment of mass at root of the neck. METHOD: Twenty-six cases of mass in root of the neck, 10 benign tumor and 16 malignancy. All the patients were received surgical treatment with "T" or "L" shaped incision, and cleidotomy or remove partial of presternum and articulationes sternoclavicularis when necessary. RESULT: All the benign tumor were removed completely, 1 case with chylous leakage and 1 case with recurrent laryngeal nerve injury after operation. Twelve cases of malignant tumor removed completely and 4 cases underwent partially resection. Subclavian vein rupture occur in 2 cases during operation, and chylous leakage in 1 case, brachial plexus or phrenic nerve injury in 2 cases individually, 1 case with mediastinal emphysema and 2 cases with hematoma. Follow up for 1 to more than 5 years. No patient died and relapsed in benign group. Six malignant cases survive for 5 years after operation,4 cases survive for more than 3 years,and 2 patients alive for 2 years. And the others were died within 1 year in malignancy group. CONCLUSION Surgery for mass in root of the neck is feasible and with good effect for benign mass,and also is a way to treat the malignancy in this region.
Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Head and Neck Neoplasms ; surgery ; Humans ; Infant ; Male ; Middle Aged ; Neck ; pathology ; surgery ; Treatment Outcome ; Young Adult

OBJECTIVE: To introduce a new transcranial endoscopic approach of glossopharyngeal neurotomy to treat glossopharyngeal neuralgia. METHOD: The clinical data, the operative technique, the operative effect and the result of follow-up of two patients were presented. Through reviewing pertinent literature, we discussed the therapeutic advancement of glossopharyngeal neuralgia and the preponderance of transcranial endoscopic approach of glossopharyngeal neurotomy. RESULT: The two patients both have satisfied outcomes and remained free of disease after surgical treatment. The endoscopic surgery has many significant advantages, such as rigorous operation, little injury, precise therapeutic effect, few complication, and so on. CONCLUSION Transcranial endoscopic approach of glossopharyngeal neurotomy is an ideal method to treat glossopharyngeal neuralgia.
Endoscopy ; Female ; Glossopharyngeal Nerve ; surgery ; Glossopharyngeal Nerve Diseases ; surgery ; Humans ; Male ; Middle Aged ; Neurosurgical Procedures ; methods ; Treatment Outcome