AIM: To assess the applied value of color Doppler ultrasound in continued monitoring the postoperative complications of renal transplantation by observing the renal morphous and related hemodynamic index of patients fore-and-aft three months of renal transplantation. METHODS: The experiment was carried out among 152 patients underwent allograft renal transplantation in Ultrasound Department of Nanfang Hospital from April 2005 to April 2006. With the informed consents, all the patients received pre-operative evaluation and postoperative monitor, emphasizing on the renal morphous, hemodynamic index and complications after renal transplantation in three days, seven days, one month and three months. First, two-dimensional imaging was used to observe the shape of the transplant kidneys and measure length, width, thickness, cortical thickness and pyramid size of kidney. Secondly, color Doppler ultrasound was used to observe the iliac artery of the transplant kidneys and the anastomose of the renal artery as well as segmental artery of kidney. And then, high frequency probe was used to check the interlobar and interlobular artery of kidney. Finally, pulsed Doppler was used to collect spectra and measure the peak systolic velocity (PSV), end-diastolic velocity, resistance index and pulsate index. RESULTS: All 152 patients were involved in the result analysis without any drop. There were 64 cases presented complications with an incidence of 42.11%. Partly of them were found more than two complications.①It included 23 cases with acute rejection, accounting for 15.14%. The resistance index of each renal artery was all more than 0.75, thereinto, 12 cases more than 0.85.②There were 14 cases renal artery stenosis, taking up to 9.22%. All of the PSV were more than 180 cm/s, thereinto, there were 5 cases (35.71%) more than 300 cm/s, 6 cases (42.86%) intercurrent acute rejection.③There were 25 cases hydronephrosis, with the incidence of 16.45%. The size of the transplant kidney was (208.52?42.43) cm3. And 20 cases showed peritransplant fluid collection with the incidence of 13.16% CONCLUSION: Color Doppler ultrasound is a useful tool for early diagnosis of blood perfusion, hemodynamic index and complication after renal transplantation; it also can improve the renal transplantation and patient survival rates successfully.

Purpose To explore the significance of stomach full acoustic imaging in the diagnosis of congenital hypertrophic pyloric stenosis (CHPS). Materials and Methods 44 cases of CHPS children underwent gastrointestinal filling acoustic contrast imaging, diameter and mucous layer thickness of the front and rear walls of the pyloric tube were measured, the contrast agent flow between the pyloric lumen gaps was observed. Results With the backdrop of the ultrasound contrast agent, delicate structure of the stomach and pyloric mucosa could be more clearly observed, it was shown that muscular layer in the middle of pyloric tube was thickest and mucosal layer edema and thickening was more obvious near the antrum;internal diameter of pyloric tube was (1.95±0.35) mm;first pass time of the contrast agent through the pyloric tube:within 5 min in 16 cases, 5-10 min in 19 cases, while clear pass through still could not be observed by 20 minutes in 9 cases;during the 20 minutes observation, contrast agent passed through the pyloric tube for 5 times or more in 13 cases, 1 to 5 times in 22 cases, while 9 cases showed no obvious pass. Conclusion Stomach full acoustic imaging enables clearly and detailed observation of the structural characteristics of the pyloric tube and hydrodynamic manifestation of the stomach liquid when passing through the tube, thus is significantly important for surgical treatment and efficacy evaluation.

Objective To explore the effects on dogs' testis by microbubbles excitated by ultrasound.Methods Sixteen male dogs were divided into 3 groups randomly.Pulsed ultrasound irradiation and intravenous microbubbles injection were both applied in the microbubble enhanced ultrasound group (MEUS),pulsed ultrasound irradiation and intravenous microbubbles injection were individually applied in the ultrasound group (TUS) and the simple microbubbles group (MB).Results In MEUS group,the layers of seminiferous tubules decreased and the germ cells were arranged disorderly and bubbles appeared in the cells.The appearances of focal ultra-structural damage such as intercellular space widening,baseline broken and tight-connection of sustentacular cells disappearing were observed by transmission electron microscope,which were slightly or hardly demonstrated in other groups.Conclusions Microbubbles excitated by ultrasound can affect testicular germ cells and the integrity of bloold-testis harrier,which providing new train of thought of target treatments of testis diseases.

ObjectiveTo investigate the haemostatic effect of combining pulsed therapeutic ultrasound (TUS) and microbubble (MB) on splenic trauma.MethodsForteen healthy dogs were divided into 3 groups.Six animals were treated by microbubble-enhanced therapeutic ultrasound (MEUS),the other eight animals were treated with TUS only ( n =4) and the MB only ( n =4) served aS the controls.The spleens of all animals were surgically exposed and a 20 mm long,5 mm deep incision was created on the spleens using scalpel.Human prothrombin complex (HPC) was slowly injected prior to the treatment.During the procedure,the initial bleeding rate and the bleeding rate after the treatment were calculated using gauze weighing.Contrast enhanced ultrasound (CEUS) was performed to assess the blocking effects of splenic circulation.The targeted spleens were harvested for pathological examination.ResultsThere were no differences in initial bleeding rate before treatment.After treatment,in the MEUS group oozed blood was found and the bleeding rate were lower than that of the TUS and the MB groups ( P ＜0.05).A nonenhanced or perfusion defect region was formed within the treated area.The histological results showed splenic sinus hyperemia,platelet aggregation and intravascular thrombosis.Conclusions Microbubbles enhanced pulsed therapeutic ultrasound can arrest the regional blood circulation of spleen by disrupting the microvasculature and this can be used in splenic heamostasis for treating spleen trauma.

Purpose To explore the value of Doppler ultrasound in the diagnosis of congenital hypertrophic pyloric stenosis (CHPS) by observing pylorus. Materials and Methods Sixyt-five children with CHPS diagnosed by ultrasound in Neonatology Department of Guangzhou First People's Hospital from 2006 to 2015 were retrospectively analyzed. CHPS group and 50 infants in control group were examined by Doppler ultrasound to observe the features of blood flow distribution and blood flow grading, and Doppler spectrum analysis was performed to observe contrast agent passing through pyloric canal. Results The difference of the thickness of muscular layer and mucous layer, and that of the long diameter and inner diameter of pyloric canal in CHPS group and control group all suggested marked statistical significance (P<0.01); the blood flow grading in muscular layer and mucous layer between the two groups showed statistical significance (t=13.33 and 18.77, all P<0.01). The blood flow velocity in muscular layer of CHPS group was (16.96±0.91) cm/s, resistance index (RI) 0.68±0.33, inner diameter of pyloric canal (1.98±0.33) mm. Conclusion Doppler ultrasound enables us to grasp the distribution and grading of blood flow of pylorus as well as the condition of contrast agent passing through pyloric canal, in which way the degree of pyloric stenosis can be evaluated by combining indexes such as Vmax and RI, and an objective basis for clinical choice of treatment can be provided, possessing high application value.