Objective:Study the relationship of HLA I and B7 1 to elucidate the role of costimulatory molecule on antigen presentation in immunological rejection.Methods:FCM was applied to detect the expression of B7 1 Then the cytotoxicity of PBML was observed before and after blocking the HLA I Results:The level of HLA I expression increased greatly(P

Objective To describe the epidemiological characteristics and relative factors of adults with overweight and obesity in Liaoning province.Methods With multi-stage randomized cluster sampling,8 120 cases were selected from the 540 families in seven urban and suburb districts of Liaoning province.data of overweight and obesity history and social- economic status for residents aged 18 yrs and over were investigated by face-to-face interview,weight and height of these subjects were measured.Result the overweight rate was 33.9%,the obesity rate was 13.7%.The prevalent rates of overweight and obesity were increased with the age and it was higher in city than in rural and in female than in male(all P

Survivin gene is the strongest inhibitor of apoptosis so far.Recently,it was found that the promoter polymorphism of survivin gene is closely related to risk of cancer and genetic susceptibility.This finding might provide clues for further elucidation of the correlation of risk of cancer and genetic polymorphism,and for diagnosis,prevention and screening of cancer gene.

MicroRNAs (miRNAs) regulate target genes expression in the post-transcriptional levels.There are many kinds of differential expressions of miRNA in head and neck cancer,and the miRNA participate in a series of important biological processes such as cell development,proliferation,differentiation,apoptosis and etc.miRNAs is expected to become an effective means for the diagnosis and treatment of head and neck cancer.

Livin is a new and important member of the inhibitor of apoptosis protein(IAPs)family.Livin is high expressed in most human tumor cells, involved in apoptosis inhibition and closely related with tumor development and progression.Studies found that through the down-regulation or inhibition of Livin expression and the induction specific antibodies of Livin could inhibi and kill tumor cells, which provided a new method for tumors'diagnosis, cell and gene targeted therapy.

The leucoanthocyantin reducase (LAR) gene, an important functional gene of catechins biosynthesis pathway, was cloned from Fagopyrum dibotrys (D.Don) Hara by degenerate PCR and rapid amplification of cDNA ends (RACE). The full-length cDNA of FdLAR is 1 581 bp (GenBank accession: JN793953), containing a 1 176 bp ORF encoding a 391 amino acids protein, and its 3'-untranslated region has an obvious polyadenylation signal. The recombinant plasmid containing FdLAR completed ORF was transformed into E. coli BL21 (DE3). The target fusion peptide with molecular weight of 66 kD was expressed under the condition of 16 degrees C and induced by IPTG at final concentration of 1.0 mmol x L(-1). Bioinformation analysis indicated that the amino acid sequence of FdLAR showed great homology to other LAR with the NADB-Rossmann conversed domain in the N-terminus. Real-time quantitative PCR was used to detect the expression levels of FdLAR gene during different development periods. The determination of flavonoids contents in appropriate rhizomes showed that the relationship between FdLAR gene expression and the accumulation of flavonoids displayed different trends during vegetative growth and reproductive growth stages, suggesting that the FdLAR gene may be involved in the pathway of flavonoid metabolisms in Fagopyrum dibotrys.

OBJECTIVE To evaluate the curative effects of surgery combined with early radiotherapy and local injection of hormonal anti-scarring drugs in the treatment of keloid in head and neck region. METHODS 35 patients with keloid were treated with surgery(completely excised and sutured directly or treated with local skin flap).Radiotherapy was applied within 24 hours after operation.A total dose of 20 Gy was administered in ten fractions of 2 Gy every day. Patients who had recurrences were treated with local injection of hormonal anti-scarring drugs.RESULTS After operation,all the wounds were healed by first intention.After 1-4 year follow-up,25 cases(71.43%) were cured without relapse;10 cases(28.57%) showed partial remission.The rate of therapeutic effect was 100%.CONCLUSION Surgical removal combined with local radiation and local injection of hormonal anti-scarring drugs are one of the effective methods in treatment with keloid in head and neck region.

OBJECTIVE:To investigate the dosage and consumption sum of opioid analgesic drug in Qingdao district. METH-ODS:The consumption data of drugs in 29 public hospitals at secondary or above level in Qingdao district were analyzed statistical-ly by ABC analytic methods and defined daily dose methods. RESULTS:In ABC analysis,5 kinds of class A drugs accounted for 20% of the total number of species,and the percentage of consumption sum was 77.38%;4 kinds of class B drugs accounted for 16.00% of all species numbers,and the percentage of consumption sum was 11.98%;other 16 kinds of drugs accounted for 64.00% of the total number of species,and the percentage of consumption sum was 10.64%. Oxycodone sustained-release tablets and Morphine sustained-release tablets with the highest DDDs consumed more health care costs,serial number ratio was 1.00,syn-chronization was good and conform to the actual needs of clinical work. CONCLUSIONS:The composition ratio of opioid analge-sic drug costs is consistent with the theoretical value,significant discrepancy between cost and DDDs does not appear.

Objective To explore the immune function of the whole body and the tumor site of human nasopharyngeal carcinoma (NPC) patients as well as its correlation with CCR4. Methods The ratios of CD4~+CD25~+T cells and CCR4+cells to lymphocytes were measured by flow cytometry in tissues (25 cases) and peripheral blood (35 cases) from nasopharyngeal carcinoma patients, and then statistical analysis was made. Results The ratios of CD4~+CD25~+T cells and CCR4+ cells in tissue and peripheral blood of NPC were higher than those in the control group (P<0.05). In NPC the ratios of the two cells in tissue were higher than in peripheral blood respectively (P<0.05), but there was no such difference between tissue and peripheral blood in the control group (P>0.05). The ratio of CD4~+CD25~+T cells in tissue at stage Ⅲ+Ⅳ was higher than that at stage Ⅰ+Ⅱ of NPC (P<0.05), but there was no such difference between the two stages in peripheral blood in NPC. There was a positive correlation between CD4~+CD25~+T cells and CCR4+ cells in tissue and peripheral blood of NPC (P<0.05). Conclusion NPC patients have different immunosuppression, and there is even more severe immunosuppression in the tumor site. The ratio of CD4~+CD25~+T cells is correlated with the stage of NPC. Therefore, as NPC progresses to later stages, the percentage of CD4~+CD25~+T cells is increased, which is correlated with poor prognosis. CCR4 plays an important role in reactant of CD4~+CD25~+T cells to tumor sites, and is resistant to immunosurveillance.