Objective To investigate the clinical characteristics and risk factors of peripheral arterial disease(PAD)in patients with diabetic foot ulcer(DFU).Methods Two hundred and eleven hospital patients with DFU were enrolled and divided into is chemic ulcer (IU) group (136 patients,with PAD) and non-IU group(75 patients,without PAD).The clinical characteristics were retrospectively analyzed.Results Single factor analysis showed that age,the course of diabetes mellitus,smoking,accompanied with coronary artery disease,the course of DFU,triacylglycerol,low-density lipoprotein cholesterol,fasting plasma glucose (FPG),post-prandial 2 h blood glucose,apaprotein(Apo)A1 and ApoB,glycosylated hemoglobin (HbA1c),systolic blood pressure,C-reactive protein were risk factors (P ＜0.01 or ＜0.05).Multiple factors Logistic regression showed that age(OR =0.983,P =0.006),accompanied with coronary artery disease(OR =4.800,P=0.001),FPG (OR =1.153,P=0.001),HbA1c (OR =0.798,P=0.001) were important risk factors of DFU.Conclusions The degree of lesion in DFU combined with PAD is more seriously and associated disease are more.Age,accompanied with coronary artery disease,FPG,HbA1c are the important risk factors of DFU combined with PAD.

OBJECTIVE To explore the risk factors and control strategies of nosocomial infection in clinical immunology laboratory.METHODS The positive detection rates of HBV marker and antibodies to HAV,HCV,HEV,HIV,TP,and TB in total of 91 877 samples in clinical immunology laboratory during Jul 2004 and Jul 2005 were retrospectively surveyed and statistically analyzed.RESULTS The number of positive specimens of HBV marker and antibodies to HAV,HCV,HEV,HIV,TP,and TB were respectively 8 376,7,26,24,107,3,522,and 52 and the positive detection rates of these items were respectively 16.68%,0.43%,0.20%,2.22%,6.49%,0.03%,4.75%,and 4.61%.CONCLUSIONS It is very important to understand the infection sources and risk factors in clinical immunology laboratory in order to strengthen management of hospital infection and protection of occupational exposure.

Objective To study the effec of nursing intervention on the quality of life in convalescent schizophrenia patients. Method 80 patients with schizophrenia were divided into observed group (n=40) and control group (n=40).The patients in observe group received nursing intervention.Patients were evaluated by Positive and Negative Symptoms Scale (PANSS) and General Quality of Life Inventory (GQOLI) at the time of beginning the trial and after half of a year of the intervention respectively. Results After the nursing intervention,the PANSS scores in observe group are significant lower than those of control group (P

OBJECTIVE: To offer guidance for psychiatric patients about rational drug use and improve patient's compliance.METHODS: Clinical pharmacists answered the questions encountered by patients during medication process by means of face-to-face talk with patients or their relatives and telephone inquiry.RESULTS: Patients' drug counseling in psychiatry department covered wide range,much as in the aspects of drug interactions and adverse drug reactions.Due to medication consultation service,patient's consciousness of rational drug use had been improved and some medication errors had been avoided,clinical pharmacists' professional knowledge has been enriched and clinical pharmacy has been enhanced and developed.CONCLUSION: The medication consultation service developed in psychiatric department is of positive practical significance in promoting rational drug use and improving clinical efficacy.

To study the effects of mouse cytomegalovirus (MCMV) on the in vitro maturation, fertilization, cleavage and blastula formation of mouse oocytes, the immature oocytes were infected in vitro by MCMVs of different dosages (100 TCID(50), 10 TCID(50) and 1 TCID(50)). The oocytes were then observed for in vitro maturation, fertilization, cleavage and blastula formation and the ultrastructural changes after the culture with the viruses. Our results showed that no significant differences were found in IVM, IVF, cleavage and blastula formation among the groups treated with of virus of various dosages. And ultrastructural abnormality was observed in the oocytes treated by 100 TCID(50) of viruses. It is concluded that MCMV did not have any conspicuous effects on IVM, IVF, cleavage and blastula formation of murine immature oocytes.
Blastocyst ; Cells, Cultured ; Cleavage Stage, Ovum ; Cytomegalovirus Infections ; Fertilization ; Muromegalovirus/*pathogenicity ; Oocytes/cytology ; Oocytes/growth & development ; Oocytes/*virology

Objective To study the forensic application of G oldeneyeTM D N A ID 26Y K it in the She na-tionality. Methods Through capillary electrophoresis, the genotype of 26 Y-STR loci were analyzed in 53 unrelated m ale individuals from Fujian She nationality. The population genetics param eters such as allele frequency and haplotype diversity were calculated. The com parisons am ong the She nationality and the other nationalities were analyzed. Results A total of 126 alleles were observed on the 26 Y-STR loci of 53 unrelated m ale individuals. The allele frequencies and G D value ranged from 0.010 1 to 0.886 8 and 0.211 2 to 0.846 2, respectively. The G D value w as greater than 0.5 in the 19 loci. A total of 47 hap-lotypes were observed. B ased on RST, m ultidim ensional scaling plot indicated that the genetic relationship am ong Fujian She nationality and M innan H an nationality w as closest, follow ed by Southern C hina H an nationality and N orthern C hina nationality. Conclusion G oldeneyeTM D N A ID 26Y K it including 26 Y-STR loci has good polym orphism in the She nationality. A s an additional system , it has forensic appli-cation value in som e special cases.

Objectives:To study the clinical characteristics of myopia in children and the measures to prevent it. Methods:748 patients with myopia were analyzed by refraction examination. Results:By the classification of the causes of myopia, the simple myopia constitutes the majority. By the degrees of the disease, the number of mild myopia was the first and moderate myopia was the second. By the patterns,the amount of compound myopic astigmatism was the majority. The correction of simple myopia or mild and moderate myopia was the most effective way. Of the patients(4～14 years), the age of 12 years had the highest myopia rate. Conclusions:To pay great attention to the prevention works of myopia in grade school years. Correcting refraction exactly and on time is an effective method to slow down the progression of myopia in children.

Objecfive To investigate the expression of erbB4 and PTEN in cutaneous squarnous cell carcinoma(SCC)tissue and their significance.Methods Immunohistochemical Elivision method was used to measure the protein expression of erbB4 and PTEN in tissue specimens from 52 patients with SCC (11 cases with and 41 cases without lymph node metastasis)and 10 normal human controls.Results The positivity rate of erbB4 was significantly higher in the patients with SCC than in the normal human controls (75%vs.10%,x2=12.77,P<0.01),and in the patients with lymph node metastasis than in those without (100%vs.68.29%,P<0.05).Significant differences were also observed in the expression rate of PTEN between the patients with SCC and the normal controls (48.08%vs.100%,x2=9.20,P<0.01),between the patients with well-differentiated SCC and those with poody differentiated SCC(78.57%vs.36.84%,P<0.05),and between the patients with lymph node metastasis and those without (9.09%vs.58.54%,P<0.01).The expression of erbB4 was negatively correlated with that of PTEN (r=-0.42.P<0.01).Conclusion The disturbalice in mutual regulation between erbB4 and FIEN seems to be involved in the initiation.progression and metastasis of SCC.

Objective To observe the inhibitory effect of pyridoxine hydrochloride (PN) combined with common chemotherapeutics on mice hepatoma cells H22 in vitro. Methods MTT assay was used to determine the effects of PN in combination with 10 different antineoplastic agents on H22 cells, and immuno-histochemistry was used to observe the distribution of PN in H22 cells and morphologic changes of the cells before and after PN treatment. Results After 24 hours incubation with 5 mmol/L PN, the treated cells expanded apparently with nucleus chipping. PN entered the tumor cell and was mainly condensed in cytoplasma and H22 cells were sensitive to PN. When administered concomitantly with chemotherapic agents, most of the combinations showed antagonistic effects while a few of the combinations were additive. For instance, doxorubicin (ADM) used in combination with PN inhibited cell proliferation with an IR value (IR=0.63) much lower than ADM alone (IR=0.71, P<0.01), and the CI value was less than 0.9, which indicated an antagonistic effect. However, PN in combination with ifosfamide (ICTX) showed additive effect (CI>0.9), and the IR value (IR=0.60) in combined group was higher than that (IR=0.40) in ICTX group (P<0.05). Conclusion PN treatment could increase the intracellular PLP level and result in growth inhibition and cell death, and combined administration of PN and ICTX might be a potential method to improve efficacy and to reduce toxic effects while a co-administration of PN and ADM should be avoided.

Objective To investigate the location and expression of hypoxia inducible factor (HIF) subunits in the remnant kidney of 5/6 nephrectomy rats. Methods Remnant kidneys were produced in adult male SD rats by 5/6 nephrectomy. The renal function and histopathological changes were evaluated at week 1, 2, 4, 6, 8 and 12 after operation. Tissues of remnant kidneys were collected to detect the location and expression of HIF-1α and HIF-2α by immunohistochemistry staining and Western blotting. The mRNA levels of HIF targeted genes vascular endothelial growth factor (VEGF) and heme oxygenase-1 (HO-1) were determined by RTPCR. Results (1) 5/6 nephrectomy rats underwent one week of acute renal failure at first[Scr (122.8±22.1) μmol/L] and then developed compensative chronic renal failure [(66.0±3.7)-(66.4±8.4) μmol/L], but the level of Scr increased quickly after week 6 [(66.4±8.4)-(127.8±22.7) μmol/L],concomitantly with progressive tubulointerstitial fibrosis in remnant kidney cortex. (2) In cortex, HIF-1α was expressed only in the atrophic and dilated tubular cells while HIF-2α was located in endothelial, interstitial fibroblasts, and vascular smooth muscle cells. The semiquantitative results of imunohistochemistry and Western blotting revealed that HIF-1α and HIF-2α were both gradually up-regulated during the early stage of remnant kidney, peaked at week 4 and 6, and then gradually down-regulated. (3) The mRNA levels of HIF targeted genes VEGF and HO-1 transiently peeked at week 4 and 6, and then decreased gradually. Conclusions The increased stabilization of HIF-αprotein and transcription of HIF targeted genes at the early stage of this model is a compensation reaction towards hypoxia. The mechanism of decreased expression of HIF-α at the end stage of chronic kidney disease deserves further investigation.