Objective: To design a heart rate testing procedure which based on Labview. Methods:Collect the blood oxygen signals of the fingers, which keep in step with the pulse wave. Filter the signals and obtain the pregnant datum by the Labview procedures. Results: Achieve the pulse cycle“T”by Labview processing the blood oxygen signals of the fingers, and gain the heart rate by calculating the pulse in one minute(Pulse is equal to the number of heart beat in one minutes-1/T,the heart rate). Conclusion:It is an important and meaningful work to process the signals. Not only acquire the heart rate, but also prompt and diagnose the diseases of the cardiovascular system. It also can provide some meaningful diagnostic evidence.

0.05). But after 12 weeks, blood glucose level and urine volume in DM group were higher and greater than those in control group (both P0.05). (2) The urine osmolality in DM group was decreased while the plasma osmolality increased (P

Objective To study the relation between the regulation of mannose‐binding lectin (MBL) level and pulmonary tuber‐culosis(TB) susceptibility .Methods A total of 142 inpatients with pulmonary TB and 120 healthy controls were recruited to par‐ticipate in this case‐control study .Serum MBL level was detected ,meanwhile the restriction fragment length polymorphism (RFLP) was adopted to detect MBL2 gene polymorphism .Results The one‐way analysis of variance was adopted to analyze the MBL level in different genotype groups ,including the group YA/YA ,XA/YA ,XA/XA ,YA/YB ,XA/YB and YB/YB ,it was found that the MBL level had statistical differences among 3 groups and between any two groups(P<0 .01) .The MBL level in the pulmonary TB group was significantly higher than that in the healthy control group (P< 0 .01) .Among the healthy controls ,the MBL level in 93 .2% individuals(55/59) carrying YA/YA genotype was>1 000 ng/mL ,while which in 100% individuals(26/26) carrying geno‐type XA/XA or allele B was≤1 000 ng/mL .Conclusion The MBL level may be associated with the susceptibility to pulmonary TB .The YA/YA gene for determining high MBL level may be a protected gene .

0.05).Conclusion The polymorphism of rs2285676 in KCNJ11 may not be associated with T2DM in Hanethnic population in Chongqing.

OBJECTIVE:To observe the clinical efficacy and safety of Qingjin huatan decoction for patients with AECOPD phlegm-heat obstructing lung. METHODS:120 patients with AECOPD phlegm-heat obstructing lung were divided into observation group and control group according to random number table,with 60 cases in each group. Control group received routine therapy as low-flow oxygen therapy,anti-infective,bronchiectasis,expectorant,etc.;observation group additionally received Qingjin huatan decoction(decocted by 400 ml pure water),tid,on the basis of control group. The levels of IL-8,IL-17,IL-23 and TNF-α were observed in 2 groups before and 7 d after treatment. Clinical efficacies and the occurrence of ADR were compared between 2 groups. RESULTS:Before treatment,there was no statistical significance in the levels of IL-8,IL-17,IL-23 and TNF-α between 2 groups (P>0.05). After treatment,the levels of IL-8,IL-17,IL-23 and TNF-α in observation group were significantly decreased and lower than in control group,with statistical significance (P<0.05). The levels of IL-17,IL-23 and TNF-α in control group were decreased significantly compared to before treatment,with statistical significance(P<0.05);the level of IL-8 had no signifi-cant change compared to before treatment,without statistical significance (P>0.05). Excellent rate of observation group was 95.00%,which was significantly higher than 78.95% of control group,with statistical significance(P<0.05). There was no statis-tical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Qingjin huatan decoction shows signifi-cant therapeutic efficacy in the treatment of AECOPD phlegm-heat obstructing lung,and can improve inflammatory factors with good safety.

Objective To find the best cut point of FPG to predict diabetes(DM) and impaired glucose tolerance(IGT) and to study its effects on the metabolism status.Methods The ROC analysis of FPG in 3189 citizens from some area of Chongqing without a previous history of diabetes was done by OGTT in a cross-section study.The metabolic features of impaired glucose regulation(IGR) and its subcategories were analyzed according to 5.6 mmol/L and 6.1 mmol/L as the FPG threshold for IFG.Results The areas under the ROC curve were 0.899 for diabetes and 0.728 for IGT.The cut point of FPG with the best equilibrium between sensitivity and specificity was 5.6 mmol/L for diabetes and 5.2 mmol/L for IGT.Among people whose 2 hPG

Objective To investigate the correlation between the excretive level of urinary albumin(ELUA) and the diabetic retinopathy(DR) in type 2 diabetes mellitus(DM) pedigree.Methods Sixty-five type 2 diabetes mellitus families including 476 persons were enrolled in the present study.All of them were divided into normal glucose tolerance group(NGT,140 persons),impaired glucose regulation group(IGR,93 persons),diabetes mellitus group(DM,164 cases) and diabetic retinopathy group(DR,79 cases).The cases in DR group were further divided into background group(BDR,66 cases) and proliferation group(PDR,13 cases) according to the findings of oral glucose tolerance test(OGTT),fundus fluorescence angiography and fundus examination.The levels of blood pressure(BP),fasting blood glucose(FBG),postprandial blood sugar(PPBS),lipid,serum creatinine(Cr),blood urea nitrogen(BUN) and ELUA were determined.The correlation analysis and multiple regression analysis were performed between the ELUA and other parameters.Results There was an increasing trend of ELUA in the NGT,IGR,DM and DR groups,and there existed significant differences in ELUA among the four groups(P0.05).A positive correlation was found by Spearman rank correlation analysis between ELUA and diastolic blood pressure(DBP) in DR group(P

The expressions of HNF-4? and HNF-1? gene were assayed by RT-PCR in the livers of insulin-resistant rats and type 2 diabetic rats. mRNA levels of HNF-4? and HNF-1? in insulin-resistant rats were lower than those in control rats, and these levels were further decreased in diabetic rats. Changes in expressions of these 2 genes seem to be associated with type 2 diabetes mellitus.

Objective: To discover and determine the content of kaempferol in Sedum aizoon L.for the first time.Methods: Waters HPLC-MS/MS,XTerra-MS C18 (5?m,2.1?150mm) and the mobile phase consisted of acetonitrile-water-formic(40∶60∶1) were applied to find kaempferol in Sedum aizoon L.;Daojin HPLC and the SHIM-PACK VP C18(250nm?4.6nm,10?m) column were used.The mobile phase consisted of methanol-0.4% phosphoric acid solutions(59:41) with the flow rate at 1.0ml/min and the UV detector wave-length were set at 370nm.Results: Compared with standard sample,the thing that kaempferol exists in Sedum aizoon L.was confirmed.The calibration curve was in good linearity over the range of 2.0-8.0?g,and regression equation was Y=40343X-11107(r=0.9998).The average recovery rate was 102.53%,with RSD =0.92%(n=6).Conclusion: The method is simple,accurate and reproducible so it can be used to determine and analyze the content of kaempferol in Sedum aizoon L.

BACKGROUND:Because of poor biocompatibility, conventional intraocular lenses (IOLs) used to improve visual acuity can result in a higher incidence of inflammatory foreign body reactions.
OBJECTIVE:To study the biocompatibility and therapeutic effects of hirudin peptide-modified acrylic IOL on eye diseases.
METHODS:By immersion coating method, we prepared a polymethylmethacrylate IOL by the modification with hirudin polypeptides and observed its biocompatibility through the cytological observation. In this study, six New Zealand white rabbits were equivalently randomized into conventional and hirudin peptide grouppolypeptide hirudin group. Rabbits in the conventional group were given conventional lens implantation; while those in the hirudin peptide group underwent hirudin peptide-modified polymethylmethacrylate IOL.
RESULTS AND CONCLUSION:Three days after implantation of hirudin peptide-modified polymethylmethacrylate IOL, cels proliferated and spread wel on the acrylic IOL, but on the cel membrane, there were a few cels most of which were gathered sphericaly with no spreading. In the hirudin peptide group, some cels attached to the border zone migrated to the IOL surface, but did not impede the central visual field; in the conventional group, the IOL surface was stil covered by fibrotic cel membrane.Comparedwith the conventional group, the score in the SchirmerItest was increased but the breakup time of tear film decreased in the hirudin peptide group. To conclude, the polymethylmethacrylate IOL modified by hirudin peptides has good physicochemical properties and biocompatibility, increases visual acuity, and yields satisfactory outcomes in the cataract treatment, which provides a new insight into the treatment of cataract.