Livin, a novel human inhibitor of apoptosis protein family member, participates in the regulation of apoptosis through inhibition of caspases and activation of mitogen activated protein kinase ( MAPK )and other pathways. Livin is over-expressed in many tumors and plays an important role in the initiation and development of tumor, providing a promising new field of cancer research.

OBJECTIVE To summarize and analyze of various consequences caused by clinical vacuum blood sampling. METHODS The data taken from 4000 cases after with vacuum blood sampling were analyzed. RESULTS The unqualified rate of vacuum blood sampling apparatus was 0.1%. There were 12 defect blood sampling cases, and there was no puncture hurt, and infection. CONCLUSIONS Internationally standardized vacuum blood sampling is safe and reliable. A strict guideline applied in vacuum blood sampling is able to control medical cross infections.

Solutions of chitosan and carrageenan were co-reacted under various experimental conditions to form water-insoluble precipitates. DR. spectroscopic studies and the counter -ions analyses revealed that the combination of the polyions proceeded through the electrostatic interaction between the - NH+3 of chitosan and the - SO3 of carrageenan, and the mole retios of N/S in the poly-electrolyte complexes (PEC) thus prepared have large scope depending on pH, the degree of deacetyla-tion (DD) of chitosan as well as the mixin ratio of the complexing components. The observation of the PEC by scanning electron microscopy showed that there exist fibrous structures in some PEC depending on such reactive conditions as pH, DD of chitosan related with the charge densities of chitosan chains. The solubility of PEC, which is greatly affected by the DD of chitosans, and the blood compatibility have also been studied.

Objective To study the etiology of comumunity acquired pneumonia (CAP) in children of Jiaxing city and provide the basis for the clinical diagnosis and treatment.Methods An etiological study was performed on 800 young children with CAP in Jinxing First Hospital from Jan 2008 to Dec 2010.Sputum samples were collected for isolating bacteria and checking the antigen of respiratory syncytial virus ( RSV),adenovirus ( ADV),influenza virus (IV) and parainfluenza virus ( PIV ).IgM antibodies of mycoplasma pneumoniae (MP),ADV,W and PIV paired serum samples were detected.The 202 cases with positive etiology were divided into three groups:bacteria group ( 109 cases),virus group (54 cases) and MP group (39 cases).The leukocyte,C-reactive protein (CRP) and procalcitonin (PCT) were detected.Results The etiology of CAP was identified in 202 (25.25％) children of all the 800 cases,the distribution of causal agents was as follows:Streptococcus pneumoniae in 46 cases (22.77％,46/202 ),Haemophilus influenzae in 32 cases (15.84％,32/202),Staphylococcus in 5 cases (2.48％,5/202),Klebsiella pneumoniae in 8 cases (3.96％,8/202),Escherichia coli in 12 cases (5.94％,12/202),MP in 39 cases ( 19.31％,39/202),RSV in 48 cases (23.76％,48/202),ADV in 1 case (0.50％,1/202),IV in 3 cases ( 1.49％,3/202),and PIV in 2 cases ( 0.99％,2/202 ),Streptococcus pneumoniae and RSV mixed infection in 3 cases ( 1.49％,.3/202),Streptococcus pneumoniae and MP mixed infection in 3 cases ( 1.49％,3/202).RSV infection occured mainly in infants less than 1-year-old,Streptococcus pneumoniae oceured in all ages but was mostly in 1 ～ 3years,Haemophilus influenzae infection occured mainly in infants less than 3-year-old,MP infection occured mainly in infants more than six years.The PCT levels of the three groups were (7.78 ±6.14) ng/ml,(0.39 ±0.56) ng/ml,and (0.20 ±0.34) ng/ml,respectively.There were significant differences among the three groups ( P ＜ 0.05 ).Conclusion RSV infection occurs mainly in infants of age less than 1 year,Streptococcus pneumoniae and Haemophilus influenzae infection occur mainly in infants of age 1 ～3 years,while MP is the leading cause of pneumonia in children of age more than 6 years in Jiaxing city.We can decide pathogen on ages,clinical manifestation and PCT level to guide the rational use of drugs.

Objective To investigate the care measures of microspheres arterial embolization in the treatment of primary liver cancer after TACE.Methods The chemotherapy drugs were injected through the catheter superselectively inserted to the left and right hepatic arteries,microspheres and super liquefied iodipin were used for tumor embolization,preoperative and postoperative care were provided to patients.Results Through the full nursing care of 50 cases of liver cancer patients,the occurrence of adverse effects and complications after surgery were reduced,enthusiasm of patients with treatment were confirmed,and the effect of the treatment was improved.Conclusions Treatment of liver cancer by hepatic artery perfusion chemo-embolization is an effective means,using scientific methods of effective care for patients is very important.

Objective To evaluate the effects of pelvic autonomic nerve preservation(PANP) on urinary and sexual functions in postoperative patients with radical resection for rectal cancer.Methods The radical resection of rectal cancer was carried out in 256 cases of patients with rectal cancer in our hospital from January 2002 to August 2008.Patients were divided into study group (n=156) with PANP and control group (n=100) without PANP.The preserved functions of automatic micturition,male erection,ejaculation,the change of female orgasm,and local recurrence of tumor were observed in the two groups.Results The preserved functions of urination,male erection,ejaculation and female sexual orgasm in study group were significantly stronger than those in control group (P<0.01),and there Was no significant difference of local recurrence of tumors between the two groups(P>0.05).Conclusions The radical operation with PANP for rectal cancer can improve postoperative micturition and sexual function,and this method does not increase local tumor recurrence after operation.

[Objective] To study the analgesic, anti-diarrhea and anti-inflammatory effects of Wulian compound extract. [Method] To study the analgesic effect of Wulian compound extract on mice with hot-plate test, writhing test, and formaldehyde stimulation. To study the anti-inflammatory effect of Wulian compound extract by mice ear-swol en induced by xylene and abdominal cavity capil ary Permeability text .To study the anti-diarrhea effect of the Wulian compound extract with the method of diarrhea induced by castor oil and senna. [Results] Wulian compound extract can inhibit the pain caused by high fever, acetic acid and formaldehyde. Inhibit the xylene induced ear swel ing of mice and increase of celiac capil ary permeability induced by acetic acid. At the same time Wulian compound extract can prolong diarrhea latency induced by the senna and castor oil, reduce the frequency of diarrhea. [Conclusion]Compound Wulian extracts have significant antidiarrheal effect, anti-inflammatory, analgesic.

Objective:To investigate the effect of methylphenidate on the ERP waveform of children with attention deficit hyperactivity disorder(ADHD) between responders and non-responders and it's psychological significance.Methods:ERP elicited by the continuous performance test were recorded before and under methylphenidate treatment in 28 ADHD children and their behavioral performance were collected simultaneously.Based on the behavioral results,the patients were divided into two groups:responders and non-responders.Results:After medcation,the responders' behavioral performance were significantly better,amplitude of NOGO-P3(30.24?10.78?v) were also larger than that of before treatment(20.34?11.42?v,P=0.04);No significant differences were found in the behavioral performance and NOGO-P3 amplitude before and after treatment(23.51?11.97?v/25.88?13.96?v,P=0.61) in the non-responders group.The amplitude of NOGO-N2 of the two groups did not have significant difference before and under methylphenidate treatment.Off medication,there were no differences between responders and non-responders.Conclusion:Methylphenidate can improve the ability of response inhibition in responders as well as increase the amplitude of NOGO-P3 of them.

Objective To analyse the expression of p16INK4A, CyclinD1, Ki-67 proteins and HPV16/18, 31/33 in invasive squamous cell carcinoma of cervix(ISCC)and to indicate the significance of them in the occurrence and development of ISCC. Methods Using tissue microarray,in Situ Hybridization (ISH) and immunohistochemical method detected the expression of HPVI6/18, 31/33 and investigated the expression of p16INK4A, CyclinD1, Ki-67 in ISCC, CIN and NCE. Results was analysed by SPSS vision 13.0. Results The positive expression rate of HPV16/18, CyelinD1, Ki-67 in ISCC was markedly higher than that in CIN and NCE. Significant relationship was observed between p16INK4A protein expression and histological grade of the cancer. No significant correlation was observed between CyclinD1 protein expression and every clinical pathological parameters of ISCC. Conclusion High-risk HPV takes an important role in the development in ISCC. p16INK4A gene might not act as a suppressor-gene only on the occurrence in ISCC. Ki-67 can be looked as a valuable clinic index of diagnosing benign and malignant tumor.

Objective To discuss Zedoray Rhizome on the correlation between its traditional Chinese herbal nature(Property and Flavor,Cannel Tropism,Efficacy and so on)and its pharmacologic actions.Methods By searching for and summarizing the literatures on the nature and pharmacologic actions of Zedoray Rhizome recorded in CNKI,CBM,Wanfang and Vip Database from 1989 to 2007,analyzed the correlation between its herbal nature and its modern pharmacologic actions.Results The efficacies of"Qi-promoting Blood-breaking and stagnation-removing" are related with the modern pharmacologic actions of resisting tumor,improving factors of hemorheology,resisting inflammation and virus,and preventing acute liver injury,etc;the efficacy of "Warming middle energizer to relieve pain"means the function of improving activities of spleen,stomach and uterus in modern pharmacology;and the spleen channel tropism is related with and its pharmacologic action of enhancing immune activity.Conclusion There are some correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions.The correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions are waiting for the further improvements to provide the dependable basis for the clinical application.