Livin, a novel human inhibitor of apoptosis protein family member, participates in the regulation of apoptosis through inhibition of caspases and activation of mitogen activated protein kinase ( MAPK )and other pathways. Livin is over-expressed in many tumors and plays an important role in the initiation and development of tumor, providing a promising new field of cancer research.

OBJECTIVE To summarize and analyze of various consequences caused by clinical vacuum blood sampling. METHODS The data taken from 4000 cases after with vacuum blood sampling were analyzed. RESULTS The unqualified rate of vacuum blood sampling apparatus was 0.1%. There were 12 defect blood sampling cases, and there was no puncture hurt, and infection. CONCLUSIONS Internationally standardized vacuum blood sampling is safe and reliable. A strict guideline applied in vacuum blood sampling is able to control medical cross infections.

Solutions of chitosan and carrageenan were co-reacted under various experimental conditions to form water-insoluble precipitates. DR. spectroscopic studies and the counter -ions analyses revealed that the combination of the polyions proceeded through the electrostatic interaction between the - NH+3 of chitosan and the - SO3 of carrageenan, and the mole retios of N/S in the poly-electrolyte complexes (PEC) thus prepared have large scope depending on pH, the degree of deacetyla-tion (DD) of chitosan as well as the mixin ratio of the complexing components. The observation of the PEC by scanning electron microscopy showed that there exist fibrous structures in some PEC depending on such reactive conditions as pH, DD of chitosan related with the charge densities of chitosan chains. The solubility of PEC, which is greatly affected by the DD of chitosans, and the blood compatibility have also been studied.

Objective To investigate the care measures of microspheres arterial embolization in the treatment of primary liver cancer after TACE.Methods The chemotherapy drugs were injected through the catheter superselectively inserted to the left and right hepatic arteries,microspheres and super liquefied iodipin were used for tumor embolization,preoperative and postoperative care were provided to patients.Results Through the full nursing care of 50 cases of liver cancer patients,the occurrence of adverse effects and complications after surgery were reduced,enthusiasm of patients with treatment were confirmed,and the effect of the treatment was improved.Conclusions Treatment of liver cancer by hepatic artery perfusion chemo-embolization is an effective means,using scientific methods of effective care for patients is very important.

Objective To study the etiology of comumunity acquired pneumonia (CAP) in children of Jiaxing city and provide the basis for the clinical diagnosis and treatment.Methods An etiological study was performed on 800 young children with CAP in Jinxing First Hospital from Jan 2008 to Dec 2010.Sputum samples were collected for isolating bacteria and checking the antigen of respiratory syncytial virus ( RSV),adenovirus ( ADV),influenza virus (IV) and parainfluenza virus ( PIV ).IgM antibodies of mycoplasma pneumoniae (MP),ADV,W and PIV paired serum samples were detected.The 202 cases with positive etiology were divided into three groups:bacteria group ( 109 cases),virus group (54 cases) and MP group (39 cases).The leukocyte,C-reactive protein (CRP) and procalcitonin (PCT) were detected.Results The etiology of CAP was identified in 202 (25.25％) children of all the 800 cases,the distribution of causal agents was as follows:Streptococcus pneumoniae in 46 cases (22.77％,46/202 ),Haemophilus influenzae in 32 cases (15.84％,32/202),Staphylococcus in 5 cases (2.48％,5/202),Klebsiella pneumoniae in 8 cases (3.96％,8/202),Escherichia coli in 12 cases (5.94％,12/202),MP in 39 cases ( 19.31％,39/202),RSV in 48 cases (23.76％,48/202),ADV in 1 case (0.50％,1/202),IV in 3 cases ( 1.49％,3/202),and PIV in 2 cases ( 0.99％,2/202 ),Streptococcus pneumoniae and RSV mixed infection in 3 cases ( 1.49％,.3/202),Streptococcus pneumoniae and MP mixed infection in 3 cases ( 1.49％,3/202).RSV infection occured mainly in infants less than 1-year-old,Streptococcus pneumoniae oceured in all ages but was mostly in 1 ～ 3years,Haemophilus influenzae infection occured mainly in infants less than 3-year-old,MP infection occured mainly in infants more than six years.The PCT levels of the three groups were (7.78 ±6.14) ng/ml,(0.39 ±0.56) ng/ml,and (0.20 ±0.34) ng/ml,respectively.There were significant differences among the three groups ( P ＜ 0.05 ).Conclusion RSV infection occurs mainly in infants of age less than 1 year,Streptococcus pneumoniae and Haemophilus influenzae infection occur mainly in infants of age 1 ～3 years,while MP is the leading cause of pneumonia in children of age more than 6 years in Jiaxing city.We can decide pathogen on ages,clinical manifestation and PCT level to guide the rational use of drugs.

Objective To investigate the change of T-lymphocyte subgroup in children with infectious mononucleosis (IM).Methods Sixty young children with IM caused by Epstein-Barr virus (EBV) were recruited for this study in Jiaxing First Hospital from Jan 2005 to Dec 2010.Thirty-six normal children were chosen as control group.Both of the two groups were detected blood test,blood smear,T-lymphocyte subgroup ( including CD3,CD4,CD8,CD4/CD8 ),liver and kidney function test,IgM antibodies of EBV,cytomegalovirus,adenovirus,and hepatitis virus.Results Compared with the control group (53.58％ ± 5.97％,31.22％±8.72％ ),the ratio of CD3 (81.55％ ±9.04％ ) and CD8(58.13％ ± 13.35％ ) were significantly higher in IM group( P ＜0.05 ).And the ratio of CD4 ( 18.19％ ± 7.61％ ) and CD4/CD8 (0.37 ± 0.33) were significantly lower in IM group,compared with the control group (36.98％ ± 5.32％,1.98 ± 0.41 )(P ＜ 0.05 ).While the leukocyte count,the ratio of abnormal leukomonocyte,and alanine transaminase had no correlation with the decrease of CD4/CD8 ( P ＞ 0.05 ).Conclusion Children with IM caused by EBV have bad immunocompetence,and we can treat them with immunotherapy early.

Objective To evaluate the effects of pelvic autonomic nerve preservation(PANP) on urinary and sexual functions in postoperative patients with radical resection for rectal cancer.Methods The radical resection of rectal cancer was carried out in 256 cases of patients with rectal cancer in our hospital from January 2002 to August 2008.Patients were divided into study group (n=156) with PANP and control group (n=100) without PANP.The preserved functions of automatic micturition,male erection,ejaculation,the change of female orgasm,and local recurrence of tumor were observed in the two groups.Results The preserved functions of urination,male erection,ejaculation and female sexual orgasm in study group were significantly stronger than those in control group (P<0.01),and there Was no significant difference of local recurrence of tumors between the two groups(P>0.05).Conclusions The radical operation with PANP for rectal cancer can improve postoperative micturition and sexual function,and this method does not increase local tumor recurrence after operation.

Objective To supply scientific basis for the reasonable development and utilization of Rubus multibracteatus Levl.et Vant.resources.Method The total flavonoids was extracted from Rubus multibracteatus Levl.et Vant.with 60% methanol and its content was determined by spectrophotometry.The reliability and repeatability of the method were tested through stability,repeatability,accuracy and recovery experiments.Result Both standard and tested solutions had the maximum absorbance at 510 nm.The RSD values of repeatability,accuracy and recovery experiments were 2.11%,0.97% and 2.4% respectively.The results of content determination showed that the total flavonoids in Rubus multibracteatus Levl.et Vant.was 3.8%.Conclusion Extracted with 60% methanol,colored with aluminiumnitrate and determined by spectrophotometry at 510 nm were suitable for the determination of total flavonoids in Rubus multibracteatus Levl.et Vant..

Objective To analyse the expression of p16INK4A, CyclinD1, Ki-67 proteins and HPV16/18, 31/33 in invasive squamous cell carcinoma of cervix(ISCC)and to indicate the significance of them in the occurrence and development of ISCC. Methods Using tissue microarray,in Situ Hybridization (ISH) and immunohistochemical method detected the expression of HPVI6/18, 31/33 and investigated the expression of p16INK4A, CyclinD1, Ki-67 in ISCC, CIN and NCE. Results was analysed by SPSS vision 13.0. Results The positive expression rate of HPV16/18, CyelinD1, Ki-67 in ISCC was markedly higher than that in CIN and NCE. Significant relationship was observed between p16INK4A protein expression and histological grade of the cancer. No significant correlation was observed between CyclinD1 protein expression and every clinical pathological parameters of ISCC. Conclusion High-risk HPV takes an important role in the development in ISCC. p16INK4A gene might not act as a suppressor-gene only on the occurrence in ISCC. Ki-67 can be looked as a valuable clinic index of diagnosing benign and malignant tumor.

Objective To discuss Zedoray Rhizome on the correlation between its traditional Chinese herbal nature(Property and Flavor,Cannel Tropism,Efficacy and so on)and its pharmacologic actions.Methods By searching for and summarizing the literatures on the nature and pharmacologic actions of Zedoray Rhizome recorded in CNKI,CBM,Wanfang and Vip Database from 1989 to 2007,analyzed the correlation between its herbal nature and its modern pharmacologic actions.Results The efficacies of"Qi-promoting Blood-breaking and stagnation-removing" are related with the modern pharmacologic actions of resisting tumor,improving factors of hemorheology,resisting inflammation and virus,and preventing acute liver injury,etc;the efficacy of "Warming middle energizer to relieve pain"means the function of improving activities of spleen,stomach and uterus in modern pharmacology;and the spleen channel tropism is related with and its pharmacologic action of enhancing immune activity.Conclusion There are some correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions.The correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions are waiting for the further improvements to provide the dependable basis for the clinical application.