Objective To evaluate the effect of Ganoderma lucidum on drug resistance of the cisplantin-resistant ovarian cancer cell line(A2780CP).Methods The A2780CP cells were randomly divided into control group and test group.The cells in control group and test group were incubated in culture media alone and in culture media containing Ganoderma lucidum 0.5 mg/ml for 48 h respectively.The resistance index(RI) of A2780CP was determined by WST-1 assay.The expression of Akt,Bcl-2 and p53 protein was measured by Western blot.Results The RI was significantly deeressed,the expression of Akt and Bcl-2 protein was down-regulated,while p53 protein expression was up-regulated in test group as compared with control group(P<0.05).Conclusion Ganoderma lueidum can reduce the resistance of A2780 CP cells to cisplantin by down-regulating the expression of Akt and Bcl-2 and up-regulating p53 expression in A2780CP cells.

Objective To explore the similarities and differences in endoscopic and pathological characteristics between elderly and non-elderly patients with Barrett esophagus (BE). Methods Three hundred and seventy-one cases with BE were divided into elderly group (n=254) and nonelderly group (n=117). The detection rate, endoscopic findings and pathological changes were assessed. Results The detection rate of BE was 2.9% in the elderly, and 0. 9% in the non-elderly(χ2 =127.8, P＜0.01). The 112 cases (44.1%) of the elderly had reflux symptoms, and so did 87cases (66.7%) of the non-elderly (χ2 =55.9, P＜0.01). The detection rate of BE in the two groups was increased year by year from 2004 to 2008. The detection rate of ring pattern was significantly higher in elderly group than in non-elderly group (28.7% vs. 10.3%, χ2=14.5, P＜0.01). Nonelderly patients had higher rate of island pattern than elderly patients (59.5% vs. 71.7%, χ2=4.7,P＜0.05). There were significant differences in the rate of specialized intestinal metaplasia between elderly and non-elderly patients (42.1% vs. 27.4%, χ2=6.9, P＜0.01). The difference in low and medium grade intraepithelial neoplasm between the two groups had statistical significance (21.3% vs.11.1%, χ2=4.9, P＜0.05). There were two cases with adenocarcinoma in elderly group, but no case was found in non-elderly group. The detection rate of H. pylori was comparable between elderly group and non-elderly group (35.5% vs. 40.9%, χ2=0.40, P＞0.05). Conclusions The elderly patients have the 3.2 times higher detection rate of BE than non-elderly patients. The detection rates of specialized intestinal metaplasia and intraepithelial neoplasm are higher in elderly group than in nonelderly group.

Objective To investigate the scientific and objective quality management methods of ophthalmic nursing to improve the quality of ophthalmic clinical nursing. Methods According to the implemention details of review standards of tertiary special eye hospital (2011) and the management specification of clinical nursing of Guangdong province, combining with the characteristics of ophthalmic nursing to formulate the 21 indicators. The indicators had been applied in 12 wards to evaluate the nursing quality since 2013 each quarter. Using the Plato analysis method to analyze the data dynamically since 2013, then controlling and improving the nursing quality in view of the analysis result. The clinical nursing quality in the first half of 2013 was compared with the second half of 2013. Results The ophthalmic nursing indicators of 12 wards in the second half of 2013 were higher than the first half of 2013. Significant statistical difference was found in the implementation rate of periocular clean, cut fingernails preoperative, infusion system, identification, send medicine to the mouth, patients at high risk of falling drop of bed evaluation, wrist band awareness, patients′ satisfaction between two groups (χ2=4.115-12.308, P < 0.05 or 0.01). Conclusions Establishing and implementing the nursing quality evaluation indicators system of ophthalmology can evaluate the clinical nursing quality objectively and promote the continuous improvement of clinical nursing quality.

Objective To explore the application conditions of 22 S rule (one of the Westgard rules) in single concentration quali‐ty control .Methods The application scope of 22 S rule was used into the same batch detection of single‐concentration quality control materials .Results The 22 S rule improved the detection rate of system errors .Conclusion 22S rule should be applied to two detec‐tions within a batch when using one quality control material ,as to improve the detection of system error more efficiency .

Objective To investigate the key nursing points during and after phacoemulsification and intraocular implantation of multiple focus lens.Method Pre-and post-operative nursing was done to the patients undergoing cataract surgery for 42 eyes of 42 patients.Result All surgeries were well done without complication.The patients’visual acuity had significant improvement after operation as compared to the pre-operation(P<0.05).Conclusions Intraocular implantation with a multiple focus lens can markedly improve the patients’vision and enhance their life quality.It is important for a nurse to conduct mental care,health education and complete preoperative examination. After surgery,the disease conditions should be carefully monitored and instructs on eye protection should be done to the patients for the purpose of promoting their recovery.

OBJECTIVE To investigate factors associated with postvitrectomy endophthalmitis. METHODS Cases with postvitrectomy endophthalmitis in our hospital between 2003 and 2007 were collected and retrospectively reviewed. RESULTS All 14011 cases (eyes) underwent pars plana vitrectomy and postoperative endophthalmitis happened to seven cases (eyes). The incidence of infection was 0.047%. Confirmed microbiology was demonstrated in 4 of 7 cases (57.0%),in which Gram-positive cocci were found 75.0%. 71.4% Of cases with postvitrectomy endophthalmitis were older than 60. The infection was controlled in 6 cases after antibiotic treatment. One case with diabetes and culture confirmation of Gram-negative bacilli deteriorated even after the second vitrectomy,and evisceration was finally performed. CONCLUSIONS The predominant organism is Gram-positive cocci in postvitrectomy endophthalmitis. The risk factors include increasing age,presence of systemic diseases and immunodeficiency. Timely and effective antibiotic therapy is important to restore visual acuity for postvitrectomy endophthalmitis.

Objective To investigate the inhibition effects of c erbB 2 and c raf 1 antisense oligodeoxynucleotides (ASODN) combined transfection on the human ovarian epithelial cancer transplanted subcutaneously in nude mice Methods There were 7 groups: normal control group,c erbB 2 sense observed group,c raf 1 sense observed group,c erbB 2 antisense observed group,c raf 1 antisense observed group,whole dose combined group,half dose combined group Human ovarian epithelial cancer cells SKOV3 were treated by different oligodeoxynucleotides,then transplanted subcutaneously in nude mice,respectively The changes of tumor volume were observed and the tumor growth inhibitory rate was calculated Results There was no difference between sense observed group and normal control group There was a larger growth inhibitory rate in whole dose combined group and half dose combined group,the first time that can be detected was 13 7 days and 15 2 days,and the maximum tumor growth inhibitory rates were 61 1% and 71 3%,respectively Conclusions The results suggested that ASODN combined transfection can inhibit the tumorigenesis of ovarian epithelial cancer cells in nude mice,it may be a more useful gene therapy for the ovarian epithelial carcinoma

Objective To observe the effects of anisodamine on levels of neurotensin in the plasma and lung homogenate during acute lung injury in rats after brain trauma.Methods The level of neurotensin in the plasma and lung organizes after brain trauma and that after administration with anisodamine were determined by radioimmunoassay(RIA).Results Level of neurotensin in the plasma and lung homogenate of rat with brain trauma was obviously higher than that in normal control group but significantly lower than that in anisodamine group.Conclusion Increase of neurotensin in the plasma and lung homogenate may be due to causing lung injury after brain trauma.Anisodamine can exert protective effect on acute brain injury by reducing level of neurotensin in in the plasma and lung homogenate.

Objective: To observe the short-term changes of Body Mass Index (BMI) and Waist Circumference (WC) in junior high school students in Harbin, and to explore its association with the changes of high-sensitivity C-reactive protein (hs-CRP) in the same period. Furthermore, to analyze the value of hs-CRP to predict the BMI and WC changes in children and adolescents. Methods: Convenient sampling method was used,and the students in grades 6 and 7 in junior high school in Harbin were selected by stratified clicstering. Physical examination and serum hs-CRP were performed for two consecutive years. According to the results of the two surveys, the subjects were divided into control group (normal → normal), case group (abnormal → abnormal), improved group (abnormal → normal) and progression group (normal → abnormal). The multinomial logistic regression was used to analyze the predictive value of hs-CRP changes for BMI and WC changes. Results: The rate of overweight and obesity among samples from junior high school was 30.6%. The increment of BMI in female was more obvious than that in male(U=17 358.0，P<0.05). Both increments in BMI and WC were positively correlated with increments in hs-CRP(P<0.05). The risk of occurrence of "BMI always abnormal" and "WC always abnormal" increased sequentially in hs-CRP "normal → abnormal", "abnormal → normal" and "abnormal → abnormal", were 3.45 times, 5.98 times and 38.84 times of "BMI is always normal", respectively; and were 3.45 times, 4.26 times and 28.50 times of "WC is always normal", respectively. The risk of "BMI improvement" was 7.70 times more than that of BMI "always normal" when hs-CRP "abnormal → normal". Conclusion The prevalence of overweight and obesity in junior high school students in Harbin is high. The BMI increases faster in female from junior high school. The trends of change of BMI and WC are consistent with the trend of change of hs-CRP. The “hs-CRP change” has a good predictive value for BMI and WC development.

Objective:To study the safety of different peripherally inserted central catheter (PICC) dressing replacement frequencies in preterm infants.Method:From June 2017 to February 2020, preterm infants were enrolled in this prospective randomized controlled study. Preterm infants with PICC were randomly assigned into 7 d, 11 d and 14 d dressing replacement groups using online randomization software. Polyurethane transparent dressing and the same dressing replacement method were used in all three groups. The incidences of catheter-related bloodstream infection (CRBSI) and positive skin bacterial culture at dressing site were compared among the three groups.Result:A total of 296 cases were enrolled, including 96 cases in the 7 d group, 108 cases in the 11 d group and 92 cases in the 14 d group. The incidences of CRBSI in three groups were 2.5/1 000 catheter day in 7 d group, 1.1/1 000 catheter day in 11 d group and 0.8/1 000 catheter day in 14 d group. The incidences of catheter pathogen colonization were 1.0% in 7 d group, 0.9% in 11 d group and 0% in 14 d group. The positive rates of skin bacterial culture at dressing site were 1.0% in 7 d group, 2.8% in 11d group and 2.2% in 14 d group. The incidences of PICC exit site infection in three groups were 1.0% in 7 d group, 0.9% in 11d group and 1.1% in 14 d group and no significant differences existed among the groups ( P>0.05). Gram-positive cocci were the main bacteria [91.7% (11/12)] of CRBSI and skin bacterial culture at dressing site and gram negative bacilli accounted for 8.3% (1/12). No fungal infection were found. Conclusion:It is safe to replace the PICC dressing in premature infants as needed within 14 days if the dressing is intact without curling, bleeding and exudation.