Objective:To evaluate the efficacy of chemotherapy for advanced biliary tract carcinoma and the factors that influence sur-vival. Methods:A total of 91 cases of advanced biliary tract carcinoma from January 2010 to April 2015 were enrolled in our study. The patients' characteristics, chemotherapy regimens, and effects were analyzed. Results:We enrolled 56 males and 35 females with a me-dian age of 57 years. A total of 90 patients were assessable for their responses to first-line chemotherapy. A total of 69 patients re-ceived the GP regimen, whereas 21 patients received some other regimens. The disease control rate (DCR), median progression free survival (mPFS), and median overall survival (mOS) were 68.1%versus 52.4%, 5.10 months versus 2.50 months (P=0.025), and 13.00 months versus 7.20 months, respectively. Only 31 patients received S-1 based regimens, and 12 patients received some other regi-mens as second-line chemotherapy. The DCR, median PFS, and median OS showed no statistical differences. Only four patients re-ceived S-1 based regimen plus bevacizumab as second-line chemotherapy (median PFS 5.3 months;median OS 7 months). Hematologi-call toxicity was the most common side effect in the first-line GP regimen. The side effects of the S-1 based chemotherapy regimen was relatively less. Conclusion:The GP regimen is an effective first-line chemotherapy for advanced biliary tract carcinoma, whereas S-1 ap-pears as an effective second-line chemotherapy drug. Bevacizumab-based regimens may be effective and require further validation.

Objective To explore the mechanism that gold nanorods trigger apoptosis in cancer cells.Methods Gold nanorods was synthesized by gold seed growing method, and its characterization was detected; gold nanorods on cell proliferation-toxicity were evaluated by CCK-8 Kit and apoptosis were detected by flow; mitochondrial membrane potential were tested by JC-1 and activation of Caspase 9 and Caspase 3 were detected by western blot. Results The results found that gold nanorods had nontoxic to normal cells, but highly toxic to tumor cells; and with the increasing of gold nanorods’ working time, the percentage of apoptotic cancer cells was increasing; in addition to, normal cells’ mitochondrial membrane potential did not change, but cancer cells had a significant reduction in mitochondrial membrane potential.Conclusion This study proves that gold nanorods induce apoptosis through the mitochondrial apoptosis pathway.

ObjectiveTo explore the effect of emotional catharsis on the mental health status， coping style and satisfaction with the intervention among adolescents in Nanchong in the post-pandemic period. MethodsCompletely random sampling method was used to recruit 390 adolescents from 2 general secondary schools， 2 county secondary schools and 2 universities in Nanchong from January 2021 to April 2022. The subjects were divided into study group （n=195） and control group （n=195） by random number table method. The study group received emotional catharsis intervention. The control group received self-regulation and relaxation without any other intervention. Interventions for both groups lasted for 3 months. Before and after the intervention， the mental health status and coping style of the adolescents were assessed using the Symptom Checklist 90 （SCL-90） and the Simplified Coping Style Questionnaire （SCSQ）. Also， the participants rated the satisfaction with the intervention via self-made satisfaction questionnaire after the intervention. ResultsAfter intervention， the total SCL-90 score of study group was lower than that of control group （t=68.312， P<0.01）. In terms of SCSQ， study group scored higher on the positive coping dimension （t=30.488， P<0.01）， and lower on negative coping dimension （t=46.562， P<0.01） than those of control group. There were 190 （98.96%） cases satisfied with the intervention in the study group and 175 （89.74%） cases in the control group， the difference of intervention satisfaction rate between the two groups was statistically significant （χ²=15.321， P<0.01）. ConclusionIn the post-pandemic period， emotional catharsis may be conducive to improve the mental health status and coping style among adolescents in Nanchong， and adolescents have high levels of satisfaction with the intervention.

Objective To investigate the value of cystic adenomatoid malformation volume ratio (CVR) using prenatal ultrasound in evaluation of congenital pulmonary sequestrations (PS) prognosis in fetus.Methods Totally 88 cases of fetal PS diagnosed with prenatal ultrasound were enrolled.The fetal CVR were recorded,and the clinical outcomes were observed.The fetus were further divided into CVR≥1.6 group and CVR＜1.6 group,then the fetal hydrops rate,incidence of respiratory distress symptoms after birth and perinatal infant survival rate between two groups were compared.Results In 88 cases,prenatal ultrasound diagnosed intralobar pulmonary sequestration (ILS) type in 62 cases (62/88,70.45％),extralobar pulmonary sequestration (ELS) type I in 19 cases (19/88,21.59％),and ELS type Ⅱ in 7 cases (7/88,7.95％).There were 81 (81/88,92.05％) live births,5 (5/88,5.68％) of induction,and 2 (2/88,2.27％) of intrauterine fetal death,respectively.In 44 cases of CVR≥1.6 group,36 cases (36/44,81.82％) had respiratory symptoms after birth,and 39 (39/44,88.64％) had combined fetal hydrops.The live birth rate was 84.09％ (37/44).In 44 cases of CVR＜1.6 group,3 cases (3/44,6.82％) had respiratory symptoms after birth and 3 (3/44,6.82％) had combined fetal hydrops.The live birth rate was 100％ (44/44).The fetal hydrops rate,incidence of respiratory symptoms after birth and perinatal infant survival rate were statistically different between the two groups (all P＜0.05).Conclusion Prenatal ultrasound CVR is an effective index for screening and assessing the prognosis in fetus with PS.

ObjectiveTo evaluate the effectiveness and consistency of three commonly used early colorectal cancer screening models for advanced colorectal adenoma as a noninvasive means， and to assess the predictive value of traditional Chinese medicine （TCM） tongue images in the models. MethodsPatients diagnosed with colorectal adenoma who underwent colonoscopy and pathological examination were selected as the study participants. Basic clinical data and tongue image were collected. The prediction models of Asia-Pacific colorectal screening （APCS） model， its revision （M-APCS） and colorectal neoplasia predict （CNP） model were applied to compare the predictive effects of the three models on advanced stage adenomas of the colon， the differences in clinical data and traditional Chinese medicine tongue characteristics among patients with different degrees of adenomas， and the similarities and differences in tongue characteristics among the models. The discriminative ability of the three risk models was evaluated using the area under the curve （AUC） and receiver operating characteristic （ROC） curves. The calibration was assessed using the Kuder-Richardson coefficient and the Hosmer-Lemeshow test for consistency analysis. ResultsA total of 227 patients with adenoma were analyzed， including 104 patients （45.82%） with advanced adenoma. In the detection of advanced adenoma， those with greasy coating （70 cases， 67.3%） were higher than those without greasy coating （34 cases， 32.7%， P＜0.05）. After multivariate analysis， the odds ratio （OR） value of non-greasy coating was 0.371 （0.204~0.673， P＜0.01）， indicating that non-greasy coating was a protective factor for advanced adenomas. Among the three risk models， the detection rate of advanced adenoma in the high-risk group with APCS was the highest （63.3%）， which was 1.49 times and 2.04 times that of the medium-risk group （42.6%） and the low-risk group （31.1%， P＜0.01）. The detection rate of advanced adenomas in high-risk groups of M-APCS and CNP was slightly higher than that in moderate or low risk groups （P＞0.05）. The proportion of yellow and greasy coating in high-risk group was higher than that in the medium-risk or low-risk group （P＜0.05）. For the ability to distinguish advanced and non-advanced adenomas， the AUC of APCS was 0.629 （95% CI： 0.556~0.702） and was higher than that of M-APCS （0.591） and CNP （0.586）. In calibration evaluation， Cronbach's alpha was 0.919 （＞0.7）， which indicated that the three models were consistent. In the correlation matrix， the correlation coefficients between APCS model and M-APCS model， and CNP model were 0.794 and 0.717， respectively， and the correlation coefficients between M-APCS model and CNP model were 0.873， Hosmer-Lemeshow χ² =2.552， P＞0.05， which suggested that the three models had good calibration ability. ConclusionAll three models demonstrate the efficiency to identify advanced colorectal adenoma， and their calibration ability is considered to be good. Among the three models， the APCS exhibits the highest recognition efficiency， however， the recognition accuracy of the APCS model needs improvement. The presence of a greasy coating is identified as one of the potential predictors of advanced adenoma. Consequently， it can be considered for inclusion in the risk model of advanced colorectal adenoma to enhance the accuracy.