An improved method for the determination of Vitamin D2 in baby food (fortified dried milk) was established by using two steps of high-performance liquid chromatography (HPLC). The sample was directly saponified and extracted with benzene. The extracted unsaponifiable matter was firstly subjected to preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (3:2) as a mobile phase. This first step was for the purpose of clean-up, and a fraction containing Vitamin D2 was collected. The separate fraction was subsequently subjected to analytic HPLC with a Zorbax SIL column (straight-phase type) with isopropanol-hexane (0.8 : 99.2) as a mobile phase. The Vitamin D2 was separated from other contaminants and determined with ultraviolet spectrograph by the peak height estimated. The recovery rate was 84%, standard deviation 12.57 and coefficient of variation 4.57%. The proposed method was rapid, accurate, and suitable for the determination of baby food.

Objective To understand the kinds and content of antibiotic used in the toothpaste selling in China. Methods 10 kinds of toothpaste samples labelled to be able to prevent and/or cure some oral diseases were collected from the supermarkets, the contents of antibiotic were determined by HPLC after selected by disk diffusion susceptibility test. Results Norfloxacin was detected in one kind of toothpaste collected from several supermarkets, the content was 0.06%. Metronidazole was detected in two kinds of toothpaste, the contents were 0.01% and 0.37% respectively, no tinidazole was detected in all samples. Conclusion Some antibiotic in common use have been detected in some toothpaste sold in supermarkets in China.

Objective To investigate hand hygiene awareness and performance for caretakers of children with chemotherapy, and explore effective intervention measures. Methods For 60 caretakers of children with chemotherapy in Hematology Department, we carried out a questionnaire survey of hand hygiene, and tracked the hand washing implementation. Also we carried on a six steps hand washing technique training for the caretakers, then compared and analyzed the hand washing qualified rate before and after the technique training. Results The hand washing cognitive rate of the caretakers when they directly contacting with sick children and before feeding food or medicine for sick children were 88. 33% and 93. 33% , and the implementation rate of those situation were 76. 67% and 89. 13% respectively. In addition, the hand washing cognitive rates of before contacting sick children′s objects and after contacting other people (including other sick children) were just 21. 67% and 41. 67% , and the implementation rates were 14. 89% and 28. 95%respectively. Before and after the six steps hand washing technique training, the bacterial colony count of the caretakers hands were (82. 36 ± 53. 32)cfu/ cm2 and (16. 21 ± 18. 83)cfu/ cm2 (t = 9. 061,P < 0. 01), but after training, the pathogenic bacteria detection rate was 31. 67% and the qualified rates 61. 67% with statistical difference (χ2 = 28. 420,27. 638, respectively; P < 0. 01). Conclusions The hand hygiene cognition for caretakers of children with chemotherapy is relatively low, and the implementation and hand washing qualified rate are unacceptable. The health education propaganda of hand hygiene and hand washing technique training for caretakers should be further strengthen. Hospital should supervise and urge the hand hygiene implementation, in order to improve the hand washing qualified rate of caretakers, reduce the nosocomial infection through hand contact, and protect the health and safety of children with chemotherapy.

Objective:To construct talent training objectives and courses for undergraduate education of tropical medicine.Methods:Two rounds of questionnaire consultation were conducted among 15 experts by Delphi method. SPSS 26.0 software was used for statistical analysis, and the recovery rate, expert authority coefficient, mean of importance score, full score ratio, coefficient of variation and Kendall coordination coefficient were calculated respectively. Kendall's rank correlation test was used to analyze the degree of expert coordination, and the "boundary value method" was used to screen the indicators.Results:The effective recovery rates of the two rounds of consultation were all 100.00% and the expert authority coefficient was 0.815. The coordination coefficient was 0.25, 0.32, and 0.27, 0.36 respectively, and the significance test showed P<0.001. Finally, 11 talent training objectives and 7 courses for undergraduate education of tropical medicine were formed. Conclusions:The talent training objectives and courses for undergraduate education of tropical medicine are reasonable and reliable, which can provide theoretical support for tropical medicine talent training and have certain guiding value.

Objective To explore the influence of evidence-based nursing on the comfort level of patient with indwelling urethral catheterization.Methods Total of 300 patients with indwelling urethral catheterizationwere divided into control group and observation group according to random number table method,with 150 patients in each group,respectively.Common nursing method wasadoptedto the control group,while evidence-based nursing interventionwas applied for observation group.Relevant literature was retrieved to select the optimal nursing evidence according to the clinical experience and patients' demand,to develop specific nursing planand assess the comfort level of the patients in the two groups according to pain level,frequent urination,urgent urination,micturition desire.Results In the control group,5 patients' comfort was in level 0 ; 65 patients' comfort was in level Ⅰ; 80 patients' comfort was in level Ⅱ; 0 patients' comfort was in level Ⅲ.In the observation group,69 patients' comfort was in level 0; 47 patients' comfort was in level Ⅰ; 34 patients' comfort was in level Ⅱ; 0 patients' comfort was in level Ⅲ.The difference of comfort level of the patients in the two groups had statistical significance (U =7.42,P ＜ 0.01).Conclusions The application of evidence-based nursing intervention can improve the comfort level of patient in indwelling urethral catheterization during the retention period.

Background: In China, secondary cytoreductive surgery (SCR) has been widely used in ovarian cancer (OC) over the past two decades. Although Gynecologic Oncology Group-0213 trial did not show its overall survival benefit in first relapsed patients, the questions on patient selection and effect of subsequent targeting therapy are still open. The preliminary data from our pre-SOC1 phase II study showed that selected patients with second relapse who never received SCR at recurrence may still benefit from surgery. Moreover, poly(ADP-ribose) polymerase inhibitors (PARPi) maintenance now has been a standard care for platinum sensitive relapsed OC. To our knowledge, no published or ongoing trial is trying to answer the question if patient can benefit from a potentially complete resection combined with PARPi maintenance in OC patients with secondary recurrence. Methods SOC-3 is a multi-center, open, randomized, controlled, phase II trial of SCR followed by chemotherapy and niraparib maintenance vs chemotherapy and niraparib maintenance in patients with platinum-sensitive second relapsed OC who never received SCR at recurrence. To guarantee surgical quality, if the sites had no experience of participating in any OC-related surgical trials, the number of recurrent lesions evaluated by central-reviewed positron emission tomography–computed tomography image shouldn't be more than 3. Eligible patients are randomly assigned in a 1:1 ratio to receive either SCR followed by 6 cyclesof platinum-based chemotherapy and niraparib maintenance or 6 cycles of platinum-based chemotherapy and niraparib maintenance alone. Patients who undergo at least 4 cycles of chemotherapy and must be, in the opinion of the investigator, without disease progression, will be assigned niraparib maintenance. Major inclusion criteria are secondary relapsed OC with a platinum-free interval of no less than 6 months and a possibly complete resection. Major exclusion criteria are borderline tumors and non-epithelial ovarian malignancies, received debulking surgery at recurrence and impossible to complete resection. The sample size is 96 patients. Primary endpoint is 12-month non-progression rate.

In recent years , there are increasing articles concerning Epstein-Barr virus associated lymphoproliferative disorder (EBV+LPD), and the name of EBV +LPD is used widely.However,the meaning of EBV+LPD used is not the same , which triggered confusion of the understanding and obstacles of the communication.In order to solve this problem.Literature was reviewed with combination of our cases to clarify the concept of EBV +LPD and to expound our understanding about it .In general, it is currently accepted that EBV +LPD refers to a spectrum of lymphoid tissue diseases with EBV infection , including hyperplasia , borderline lesions , and neoplastic diseases .According to this concept , EBV+LPD should not include infectious mononucleosis ( IM ) and severe acute EBV infection ( EBV +hemophagocytic lymphohistiocytosis, fatal IM, fulminant IM, fulminant T-cell LPD), and should not include the explicitly named EBV+lymphomas ( such as extranodal NK/T cell lymphoma , aggressive NK cell leukemia , Burkitt lymphoma, and Hodgkin lymphoma , etc.) either.EBV +LPD should currently include: ( 1 ) EBV +B cell-LPD:lymphomatoid granulomatosis , EBV +immunodeficiency related LPD , chronic active EBV infection-B cell type, senile EBV +LPD, etc.(2) EBV +T/NK cell-LPD:CAEBV-T/NK cell type, hydroa vacciniforme, hypersensitivity of mosquito bite, etc.In addition, EBV+LPD is classified, based on the disease process , pathological and molecular data , as 3 grades:grade1, hyperplasia ( polymorphic lesions with polyclonal cells ); grade 2, borderline ( polymorphic lesions with clonality ); grade 3, neoplasm (monomorphic lesions with clonality).There are overlaps between EBV +LPD and typical hyperplasia, as well as EBV+LPD and typical lymphomas .However , the most important tasks are clinical vigilance , early identification of potential severe complications , and treating the patients in a timely manner to avoid serious complications , as well as the active treatment to save lives when the complications happened .