1.Auditory brainstem response and DPOAE in autism children
Sufang WANG ; Xuelei DONG ; Yongsheng WANG
Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(02):-
OBJECTIVE To explore the audiological characteristics in children with autism. METHODS Agroup of 20 children with autistic disorder were included in an investigation of auditory brain stern responses(ABR)and distortion product otoacoustic emissions(DPOAE).The results were compared with those in 18 normal children of agematched. RESULTS①As compared with the values in the control group,the latency of waveⅤfor both ears,waveⅠ-Ⅴand waveⅢ-Ⅴintervals for the right were significantly prolonged(P
2.Role of cPKCγ/GAP-43 signaling pathway in ketamine-induced apoptosis in hippocampal neurons of developing rats:an in vitro experiment
Pei ZHANG ; Zimiao HAO ; Sufang JIANG ; Xuze LI ; Lijun BO ; Rongtian KANG ; Zhenming DONG ; Lining HUANG
Chinese Journal of Anesthesiology 2017;37(3):296-299
Objective To evaluate the role of conventional protein kinase Cγ (cPKCγ)/growthassociated protein-43 (GAP-43) signaling pathway in ketamine-induced apoptosis in hippocampal neurons of developing rats in an in vitro experiment.Methods Primarily cultured hippocampal neurons were seeded in culture plates at a density of 1×10.6 cells/ml and divided into 2 groups (n=10 each) using a random number table:control group (C group) and ketamine group (K group).Group C received no treatment.Ketamine was added with the final concentration of 300 μmol/L in group K.At 12 h of culture or incubation,the apoptosis in hippocampal neurons was detected by flow cytometry.The apoptotic rate was calculated.The expression of cPKCγ,GAP-43 and phosphorylated GAP-43 in hippocampal neurons was measured by Western blot.Results Compared with group C,the apoptotic rates of hippocampal neurons were significantly increased,and the expression of cPKCγ,GAP-43 and phosphorylated GAP-43 was down-regulated in group K (P<0.01).Conclusion The mechanism by which ketamine induces apoptosis in hippocampal neurons of developing rats may be related to inhibition of cPKCγ/GAP-43 signaling pathway activation in an in vitro experiment.
3.The effect of injecting mouse nerve growth factor on the recovery of hand function in patients with cubital tunnel syndrome
Sufang HUANG ; Chaoliang WANG ; Xueguang DONG ; Li GENG
Chinese Journal of Physical Medicine and Rehabilitation 2019;41(2):106-110
Objective To observe the effectiveness of injecting mouse nerve growth factor ( mNGF) on the recovery of hand motor function among patients with cubital tunnel syndrome. Methods A total of 138 patients with moderate to severe cubital tunnel syndrome were randomly divided into groups designated as A, B and C, each of 46. Twenty micrograms of mNGF was injected daily 1 mm from the ulnar nerve at the cubital tunnel for the patients of group A and the injection site was moved 1 mm distally everyday along the nerve , but injected intramuscularly for those in group B. Those in group C received 500 μg of mecobalamin injected intramuscularly 3 times a week. The whole intervention consisted of two 4-week phases, with an interval of 2 months. Before and after the intervention, the function of internal hand muscles, hand function recovery rates and any electrophysiological changes in the ulnar nerve were measured and compared between the two groups. Results All of the patients showed significant improve-ment in hand muscle function and neuroelectrophysiology. The incidence of had muscle atrophy, Tinel′s sign, posi-tiveness in the paper clamping test and claw hand all significantly improved compared with before the treatment in all three groups. The average Disability of Arm Shoulder and Hand score in group A after the treatment was significantly higher than the group B and group C averages. The average ulnar nerve conduction velocity, incubation period and amplitude of group A after the treatment were all significantly better than before the treatment and better than the other groups′averages. After the treatment, the average hand function recovery in group A reached 91%, significantly high-er than in groups B ( 76%) and C ( 59%) . Conclusion Injecting mNGF next to the ulnar nerve is superior to in-jecting it intramuscularly in promoting the recovery of the ulnar nerve and hand function for patients with moderate to severe cubital tunnel syndrome.
4.Research on constructing talent training objectives and courses of undergraduate education of tropical medicine by Delphi method
Jie WU ; Yan LI ; Qunfang CAI ; Sufang DONG ; Li YIN ; Yajun LU ; Qianfeng XIA
Chinese Journal of Medical Education Research 2024;23(3):343-346
Objective:To construct talent training objectives and courses for undergraduate education of tropical medicine.Methods:Two rounds of questionnaire consultation were conducted among 15 experts by Delphi method. SPSS 26.0 software was used for statistical analysis, and the recovery rate, expert authority coefficient, mean of importance score, full score ratio, coefficient of variation and Kendall coordination coefficient were calculated respectively. Kendall's rank correlation test was used to analyze the degree of expert coordination, and the "boundary value method" was used to screen the indicators.Results:The effective recovery rates of the two rounds of consultation were all 100.00% and the expert authority coefficient was 0.815. The coordination coefficient was 0.25, 0.32, and 0.27, 0.36 respectively, and the significance test showed P<0.001. Finally, 11 talent training objectives and 7 courses for undergraduate education of tropical medicine were formed. Conclusions:The talent training objectives and courses for undergraduate education of tropical medicine are reasonable and reliable, which can provide theoretical support for tropical medicine talent training and have certain guiding value.
5.Analysis on the incidence and influencing factors of psychological violence among college students in Guangzhou in 2010.
Chang WANG ; Guibo CHI ; Sufang MO ; Shengyong WANG ; Jingbo ZHANG ; Xiaomei DONG ; Wenhao LI
Chinese Journal of Preventive Medicine 2014;48(10):872-877
OBJECTIVETo study the incidence and influencing factors of psychological violence among college students in Guangzhou in 2010.
METHODSConvenience sampling method was used and 2 200 college students from 61 classes of three universities in Guangzhou were interviewed with self-designed questionnaire about the incidence and influencing factors of the psychological violence in 2010. The valid sample was 2 060. Chi-square test and multinomial logistic regression were used to assess the incidence and influencing factors of the psychological violence between different genders.
RESULTSA total of 66.3% (1 365/2 060) college students reported having experienced at least one kind of psychological violence during the past 12 months, either as a perpetrator or as a victim. The proportion of psychological violence among males (72.3%, 775/1 072) was significantly higher than that in females (59.7%, 590/988)(χ(2) = 36.39, P < 0.05). The incidence of perpetration only, victimization only, and both perpetration and victimization of psychological violence were 16.1% (331/2 060), 8.9% (184/2 060) and 41.3% (850/2 060), respectively. The incidence of victimization only in females was 10.4% (103/988), which was significantly higher than that in males (7.6%, 81/1 072) (χ(2) = 5.20, P < 0.05). The incidence of both perpetration and victimization in males was 47.3% (507/1 072), which was significantly higher than that in females (34.7%, 343/988) (χ(2) = 33.56, P < 0.05). In males, the multinomial logistic regression showed the risk factors for perpetration only was having harmful behavior habits (OR = 1.90). For victimization only, the risk factors were with siblings (OR = 2.42) and prior mistreatment by teachers from primary to senior high school (OR = 1.78). For both perpetration and victimization, the risk factors were with siblings (OR = 1.88), having harmful behavior habits (OR = 1.98), bad family economic conditions (difficult OR = 1.99, general OR = 1.67) and prior mistreatment by teachers from primary to senior high school(OR = 2.07). In females, the risk factors for perpetration only were self-claimed shortage of monthly living expenses(OR = 2.14) or just enough (OR = 2.26), having harmful behavior habits (OR = 1.69) (all P values<0.05) . For victimization only, the risk factors were bad family economic conditions (difficult OR = 6.67, 15 persons; general OR = 3.81), bullied by others before university(OR = 2.05). For both perpetration and victimization, the risk factors were self-claimed shortage of monthly living expenses (OR = 1.81), bad family economic conditions (OR = 2.43), disharmonious relations between parents (OR = 1.76), physical punishment by parents (OR = 1.66), bullied by others before university (OR = 2.14) and prior mistreatment by teachers from primary to senior high school(OR = 1.73). Having a religious faith was the protective factor (OR = 0.38) (all P values<0.05).
CONCLUSIONThe incidence of psychological violence among college students is very serious in Guangzhou in 2010. And the influencing factors of it between different genders are different. Females are affected by a wider ranges of factors than males, and are more influenced by family and parents.
Adolescent ; Aggression ; classification ; psychology ; Family ; psychology ; Female ; Humans ; Incidence ; Logistic Models ; Male ; Risk Factors ; Sex Factors ; Stress, Psychological ; epidemiology ; Students ; Surveys and Questionnaires ; Universities
6.Morphological study on abnormal brain development induced by hypoxia in neonatal mice
Jie YUAN ; Yingqi LIU ; Yuhao DONG ; Yiyuan HUANG ; Sufang JIANG ; Ming ZHANG ; Xianghui ZHAO
Chinese Journal of Neuroanatomy 2023;39(6):633-640
Objective:Neonatal mice hypoxia model was established to observe the responses of the main neural cell types in cognition-related brain areas.Methods:Pups at postnatal day 2(P2)were subjected to 10%oxygen for suc-ceeding 5 days,and harvested at different development stage for histologic study.Immunofluorescence histochemistry was used to compare the changes of oligodendrocyte density,mature oligodendrocyte ratio and myelin protein level in corpus callosum(CC)and motor cortex(M1)after hypoxia,as well as the expression changes of excitatory and inhibi-tory neurons in anterior cingulate cortex(ACC),hippocampus(Hippo)and sensory cortex(S1).Furthermore,the density changes of different types of inhibitory intermediate neurons,microglia and astrocytes in ACC were compared.At the same time,the effect of hypoxia on the expression of synaptic proteins was also detected.Results:Quantitative immunofluorescence results showed lower myelin protein levels and mature oligodendrocyte ratio in CC and M1 of hypoxic mice compared with control mice.There was no significant difference in the number of excitatory neurons in ACC,but the number of gamma-aminobutyric acid(GABA)neurons in ACC,Hippo,and S1 were significantly reduced,especially parvalbumin neuron,ssomatostatin neurons,and vasoactive intestinal polypeptide neurons in ACC.The number of excitatory synapses labeled by vesicular glutamate transporter 1(VGluT1)and inhibitory synapses labeled by gephyrin were significantly decreased in ACC of hypoxic mice.Although there was no significant difference in astrocyte and microglia numbers,microglia were activated after hypoxic injury.Conclusion:Chronic hypoxia will lead to changes in the development of oligodendrocytes and interneurons,impair synapse formation.These results provide an important experimental basis for exploring the neural mechanism of diseases related to abnormal brain intelligence devel-opment.
7.Surveillance analysis of CD4+T lymphocyte levels among HIV/AIDS population before and after antiviral treatment in Xining City, Qinghai Province
Qing JIA ; Ru MA ; Shicun DONG ; Yuxin MA ; Sufang YANG ; Fuchang MA ; Chunxiang WANG
Shanghai Journal of Preventive Medicine 2024;36(9):862-867
ObjectiveTo analyze the changes in CD4+T lymphocyte (CD4 cells) levels and influencing factors in patients with human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) before and after highly active anti-retroviral therapy (HAART) in Xining City, Qinghai Province, so as to provide a scientific basis for formulating corresponding follow-up intervention strategies. MethodsFrom February to July, 2023, 468 HIV/AIDS patients who received HAART in Xining, Qinghai Province were selected using a convenient sampling method for a questionnaire survey. Venous blood was collected and CD4 cell levels were detected by flow cytometry. SPSS 22.0 was used for t-test, analysis of variance and χ2⁃test, and multiple linear regression analysis was used to explore the level of CD4 cells and its main influencing factors in HIV/AIDS population after HAART. ResultsUnivariate analysis showed significant differences in baseline CD4 cell levels among people of different ages, nationalities, residential types and annual income (P<0.05). There were statistically significant differences in the most recent CD4 cell levels among people of different nationalities, different marital statuses, with or without regular physical exercise, with or without hypertension, different baseline CD4 cell count, different years since HIV diagnosis, different years of HAART treatment, and different treatment programs (P<0.05). Multiple linear regression analysis showed that the baseline CD4 cell count was 350‒499 (t=7.40) and ≥500 (t=6.69), the number of years of HAART treatment was 5‒9 (t=3.59) and ≥10 (t=4.54), the number of years of HIV diagnosis was ≥10 (t=-2.58), the number of people receiving second-line treatment (t=-2.49) and the latest CD4 cell levels after HAART were statistically correlated (P<0.05). ConclusionThe CD4 cell levels of HIV/AIDS population in Xining city of Qinghai Province are affected by many factors. Comprehensive health intervention should be carried out for this population, the implementation of early detection and treatment strategy should be strengthened, relevant physiological and biochemical indicators should be monitored regularly, and health education should be carried out, so as to encourage this population group to choose a healthy lifestyle, improving their quality of life from multiple dimensions.
8.Antimicrobial resistance profile of clinical isolates in hospitals across China: report from the CHINET Surveillance Program, 2017
Fupin HU ; Yan GUO ; Demei ZHU ; Fu WANG ; Xiaofei JIANG ; Yingchun XU ; Xiaojiang ZHANG ; Zhaoxia ZHANG ; Ping JI ; Yi XIE ; Mei KANG ; Chuanqing WANG ; Aimin WANG ; Yuanhong XU ; Jilu SHEN ; Ziyong SUN ; Zhongju CHEN ; Yuxing NI ; Jingyong SUN ; Yunzhuo CHU ; Sufei TIAN ; Zhidong HU ; Jin LI ; Yunsong YU ; Jie LIN ; Bin SHAN ; Yan DU ; Sufang GUO ; Lianhua WEI ; Fengmei ZOU ; Hong ZHANG ; Chun WANG ; Yunjian HU ; Xiaoman AI ; Chao ZHUO ; Danhong SU ; Ruizhong WANG ; Hua FANG ; Bixia YU ; Yong ZHAO ; Ping GONG ; Dawen GUO ; Jinying ZHAO ; Wenen LIU ; Yanming LI ; Yan JIN ; Chunhong SHAO ; Kaizhen WEN ; Yirong ZHANG ; Xuesong XU ; Chao YAN ; Hua YU ; Xiangning HUANG ; Shanmei WANG ; Yafei CHU ; Lixia ZHANG ; Juan MA ; Shuping ZHOU ; Yan ZHOU ; Lei ZHU ; Jinhua MENG ; Fang DONG ; Hongyan ZHENG ; Han SHEN ; Wanqing ZHOU ; Wei JIA ; Gang LI ; Jinsong WU ; Yuemei LU
Chinese Journal of Infection and Chemotherapy 2018;18(3):241-251
Objective To investigate the antimicrobial resistance profile of the clinical isolates collected from selected hospitals across China. Methods Twenty-nine general hospitals and five children's hospitals were involved in this program. Antimicrobial susceptibility testing was carried out according to a unified protocol using Kirby-Bauer method or automated systems. Results were interpreted according to CLSI 2017 breakpoints. Results A total of 190 610 clinical isolates were collected from January to December 2017, of which gram negative organisms accounted for 70.8% (134 951/190 610) and gram positive cocci 29.2% (55 649/190 610). The prevalence of methicillin-resistant strains was 35.3% in S. aureus (MRSA) and 80.3% in coagulase negative Staphylococcus (MRCNS) on average. MR strains showed much higher resistance rates to most of the other antimicrobial agents than MS strains. However, 91.6% of MRSA strains were still susceptible to trimethoprim-sulfamethoxazole, while 86.2% of MRCNS strains were susceptible to rifampin. No staphylococcal strains were found resistant to vancomycin. E. faecalis strains showed much lower resistance rates to most of the drugs tested (except chloramphenicol) than E. faecium. Vancomycin-resistant Enterococcus (VRE) was identified in both E. faecalis and E. faecium. The identified VRE strains were mainly vanA, vanB or vanM type based on phenotype or genotype. The proportion of PSSP or PRSP strains in the non-meningitis S.pneumoniae strains isolated from children decreased but the proportion of PISP strains increased when compared to the data of 2016. Enterobacteriaceae strains were still highly susceptible to carbapenems. Overall, less than 10% of these strains (excluding Klebsiella spp.) were resistant to carbapenems. The prevalence of imipenem-resistant K. pneumoniae increased from 3.0% in 2005 to 20.9% in 2017, and meropenem-resistant K. pneumoniae increased from 2.9% in 2005 to 24.0% in 2017, more than 8-fold increase. About 66.7% and 69.3% of Acinetobacter (A. baumannii accounts for 91.5%) strains were resistant to imipenem and meropenem, respectively. Compared with the data of year 2016, P. aeruginosa strains showed decreasing resistance rate to carbapenems. Conclusions Bacterial resistance is still on the rise. It is necessary to strengthen hospital infection control and stewardship of antimicrobial agents. The communication between laboratorians and clinicians should be further improved in addition to surveillance of bacterial resistance.