Objective To investigate the self-management self-efficacy,health-related quality of life of hemodialysis patients and explore the relationship between them. Methods Self-management self-efficacy questionnaire and SF-36 were used,and 55 hemodialysis patients participated in this study. Pearson correlate and regression analysis were used to test the relationship between them. Results 55 questionnaires were available. Positively associated between self-management self-efficacy and HRQOL(r=0.268,P＜0.05);significantly positively associated between physical activity self-efficacy and HRQOL(r=0.409,P＜0.01). Regression analysis showed that physical activity self-efficacy is the factor of HRQOL (t=3.261,P＜0.01).Conclusion Physical activity self-efficacy is the factor of HRQOL in hemodialysis patients,increasing self-management self-efficacy is benefit to their HRQOL.

Objective To investigate the ability of theory of mind use in elders,and determine the role of inhibitory control in the use of theory of mind. Methods The participants included 30 young-old people ,30 oldold people and 30 young people. Their ability of theory of mind use and inhibitory control were examined with The Referential Communication Task and Hayling Sentence Completion Test,respectively. Results The correct rate of referential communication task was significantly lower in old-old people (0.43 ± 0.32) than those of young-old people (0.69 ±0.24, P=0. 001 ) and young people (0. 77 ±0. 25, P＜0. 001 ). Partial correlation analysis showed that the total errors in Hayling Test-B was negatively correlated with the correct rate of Referential Communication Task ( r= -0.442, P＜0.001 ). Hiberarchy regression analysis showed that the total errors in Hayling Test-B entered the equation ( β = -0.451, P＜0. 001 ) and could independently explained 16.8% of the variance (△R2 = 0. 168, △F = 19.359, P ＜ 0.001 ) after controlling for age. Conclusion Old-old people show worse performance in the use of theory of mind. The impairment of inhibitory control can partly explain the decline of theory of mind use in old-old people.

AIM To express recombinant human cytochrome P450 3A4 mutants CYP3A4.3, CYP3A4.4, CYP3A4.5 and CYP3A4.18, and to employ them for in vitro metabolism studies of CYP3A4. METHODS Use Bac-to-Bac baculovirus expression system to recombinant baculovirus carrying cDNA of CYP3A4 mutants CYP3A4.3, CYP3A4.4, CYP3A4.5 and CYP3A4.18. Spodoptera frugiperda 9 (Sf9), cells were co-infected by recombinant viruses of CYP3A4 mutants, human NADPH-P450 oxidoreductase and cytochrome b5 to obtain recombinant proteins CYP3A4.3, CYP3A4.4, CYP3A4.5 and CYP3A4.18 with metabolic activity. RESULTS The mRNA transcription of CYP3A4 mutants in Sf9 cells were validated by RT-PCR. Testosterone and 7-benzyloxy-4-(trifluoromethyl) coumarin were metabolized by the lysates of Sf9 cells infected by the recombinant viruses. CONCLUSION CYP3A4 mutants CYP3A4.3, CYP3A4.4, CYP3A4.5 and CYP3A4.18 with metabolic activity were successfully expressed by baculovirus-insect cell expression system. The results indicated that recombinant CYP3A4. 5 showed lower activity comparing to the wild type protein towards testosterone, while CYP3A4. 18 with higher activity, and for CYP3A4.3 and CYP3A4.4 showing similar activity to the wild type protein.

Gastrodin (GAS) is the major bioactive component of the extracts from the rhizome of Gastrodia elata Blume. The aim of this study is to investigate the transport of GAS in Caco-2 cells and the interaction of P-glycoprotein and GAS. The apparent permeability coefficients (Papp) of GAS were measured as a function of directions and concentrations. It was demonstrated that the efflux ratio was < 2.0 over the range of 50-500 micromol x L(-1) of GAS from bi-directional transport studies. The transport rate of GAS was dependent on the concentrations. Papp of GAS was not affected by transport directions, GAS concentration or the classical inhibitors of P-glycoprotein (verapamil and GF 120918). The cellular accumulation of GAS in Bcap37/MDR1 cells transected with hMDR1 gene, was similar to that in Bcap37 cells. The accumulation in both cell lines was concentration dependent. GAS did not affect the accumulation of Rhodamine 123 in Bcap37/MDR1 cells over the range of 50-500 micromol x L(-1). It indicated that the transport of GAS in Caco-2 cell monolayers mainly is by passive paracellular transport pathway. P-glycoprotein did not participate in the absorption of GAS in the intestine or the transport across the blood-brain barrier.

Child ; Clinical Laboratory Techniques ; Humans ; Tuberculosis ; diagnosis

Objective To analyze the distribution of Escherichia(E .) coli in clinical infection and its drug resistance situation to provide the scientific evidence for the control of outside hospital infection and nosocomial infection and clinical rational drug use . Methods E .coli isolated situation among various types of clinical samples in our hospital during 5 years from January 2010 to De‐cember 2014 ,its department distribution and drug resistance were analyzed .Results The isolated 2 405 strains of E .coli were mainly originated from urine samples (1 049 strains ,43 .60% ) and sputum samples (562 strains ,3 .4% ) .In which the detection rate of extended‐spectrum beta‐lactamase(ESBLs)producing E .coli was 57 .92% ;the resistance rate of E .coli to the most antibacterial drugs including penicillins ,cephalosporins ,fluroquinolones ,aminoglycosides and sulfonamides was more than 50% ,the drugs with the resistance less than 10% were imipenem(0) ,meropenem(0) ,piperacillin/tazobactam(4 .6% ) ,cefoperazone/sulbactam(6 .4% ) and cefoxitin (7 .7% ) ,the resistance to partial third and fourth generation cephalosporins of ceftriaxone ,cefotaxime and cefepime showed the significantly increasing trend .Conclusion E .coli has relatively serious drug resistance situation in hospital‐acquired in‐fections ,clinic should strengthen the pathogen distribution monitoring and drug resistance detection for avoiding the generation of more drug resistant strains in order to reduce the bacterial drug resistance and the hospital infection rate .

Antisperm antibodies (ASA) in sera from 59 couples of infertility were studied by ELISA, the positive rate was 32.20%, in which 20.34% females and 11.86% males. (P

The effects of SLG on the immune function of normal and immune-suppressed mice were investigated. The results showed that SLG (50mg/kg, im) could correct the suppressive effect of cyclophosphamide, dexamethasone or prednisolone on spleen and. thymus weight, serum specific antibody level and lymphocyte transformation. SLG could also improve the phagocytic activity of peritoneal macrophages and enhance peripheral blood ANAE+-lymphocyte ratio of mice. No obvious effect of SLG on the normal mice were observed except that it could increase the antibody level of normal mice. The results implicate that SLG may have some immunomodulatory effect.

Objective: To understand the cognition status for risk factors and early symptoms of stroke via face to face questionnaire survey in 336 in-hospital patients with atrial ifbrillation (AF).
Methods: A face to face questionnaire survey was conducted by uniformly trained investigators in 336 AF patients who were admitted in our hospital more than 3 days from 2014-04-01 to 2014-10-01.
Results: There were 300/336 AF patients ifnished the questionnaire and among them 131 (43.7%) patients know that AF may increase the risk of stroke and 169 (56.3%) patients unknown or uncertain. There were 41.3%-79.3% patients having cognition for early symptoms of stroke and only 25.3% (76/300) patients may identify all 5 early signs of stroke.
Conclusion: In our research, AF patients had poor cognition for the risk and early signs of stroke. Medical professional should intensify the propaganda and education for stroke prevention.

Objective To compare curative effects between laparoscopic oviduct-sparing operation and medical interventional treatment for the management of tubal pregnancy.Methods A total of 63 patients with tubal pregnancy were randomly divided into two groups.One group underwent laparoscopic salpingotomy and aspiration of the gestational sac content, while the other group underwent intra-tubal injection of methotrexate(MTX).Curative effects of the two procedures were compared.Results Laparoscopic oviduct-sparing operation was successfully performed on one session in 31 out of 32 patients,the success rate being 96.9%.Medical interventional treatment was conducted successfully in 24 out of 31 patients,23 of whom received one dose of MTX and 1 of whom received two doses,the success rate being 77.4%.There was no statistical difference between the two groups in success rates (?~2=3.765,P=0.052).The time for serum ?-hCG to normal levels was significantly shorter in patients receiving laparoscopic oviduct-sparing operation(9.1?2.1 d) than in patients receiving interventional treatment(26.3?3.9 d)(t=-20.974,P=0.000).Conclusions Both laparoscopic oviduct-sparing operation and medical interventional treatment can effectively preserve affected oviduct for patients with tubal pregnancy.As compared with medical interventional treatment,laparoscopic oviduct-sparing operation has a broader range of indications,less side effects,and a shorter recovery time of serum ?-hCG.