Child ; Clinical Laboratory Techniques ; Humans ; Tuberculosis ; diagnosis

Objective To investigate the correlation of serum alpha-fetoprotein (AFP) with clinical pathological factors and prognosis of hepatocellular carcinoma ( HCC ).Methods Two hundred and twenty patients with HCC who hospitalized in our hospital from January 2007 to June 2009 were enrolled in this study.The relationship of AFP levels before operation to clinical pathological factors such as pathological grade,tumor size,tumor encapsulation,tumor numbers,portal vein tumor thrombus,TNM stage and postoperative recurrence were analyzed.Results Serum AFP concentration before operation were related with tumor size ( x2 =11.76,P ＜ 0.05 ),tumor encapsulation ( x2 =17.07,P ＜ 0.05 ),portal vein tumor thrombus ( x2 =8.944,P ＜ 0.05 ),higher TNM stage ( x2 =4.929,P ＜ 0.05 ) and early postoperative recurrence rate ( x2 =5.569,P ＜ 0.05 ).However,it had no correlation with pathological edmondson grade tumor numbers.Conclusion HCC patients with high serum AFP concentration often has greater tumor without encapsulation,high occurrence rate of portal vein tumor thrombus and higher TNM stage,they are more likely to early postoperative recurrence.Serum AFP before operation is useful not only for diagnosis,but also as a prognostic biomarker to predict prognosis and recurrence.

Objective:To develop an Online Game Cognitive Distortions Scale(OGCDS) and examine its applicability in Chinese adolescents.Methods:A pretest was given to 117 middle school students to determine a 17-item version of OGCDS.A sample of 495 middle school students were required to complete OGCDS,Internet Game Cognition Addiction Scale and Young's Internet Addiction Disorder scale.Reliability and validity of OGCDS were analyzed.Results:①The retest reliability,split-half and Cronbach's ? reliability coefficients of the total scale were 0.753,0.827 and 0.904,respectively.②Scores of the total scale and each subscale were both significantly correlated with scores of IGCAS and IAD scale at 0.01 levels (r=0.540～0.728;r=0.283～0.413).③Four factors were extracted by exploratory factor analysis,explaining 72.626% of the total variance;the load of each item varied from 0.490～0.886.④Confirmatory factor analysis confirmed that standard measurement model fit data well:?2=141.586,NFI=0.909,IFI=0.952,CFI=0.952,RMSEA=0.063.Conclusion:Reliability and validity of OGCDS is corresponded with psychometric standard.

Objective: To understand the cognition status for risk factors and early symptoms of stroke via face to face questionnaire survey in 336 in-hospital patients with atrial ifbrillation (AF).
Methods: A face to face questionnaire survey was conducted by uniformly trained investigators in 336 AF patients who were admitted in our hospital more than 3 days from 2014-04-01 to 2014-10-01.
Results: There were 300/336 AF patients ifnished the questionnaire and among them 131 (43.7%) patients know that AF may increase the risk of stroke and 169 (56.3%) patients unknown or uncertain. There were 41.3%-79.3% patients having cognition for early symptoms of stroke and only 25.3% (76/300) patients may identify all 5 early signs of stroke.
Conclusion: In our research, AF patients had poor cognition for the risk and early signs of stroke. Medical professional should intensify the propaganda and education for stroke prevention.

Antisperm antibodies (ASA) in sera from 59 couples of infertility were studied by ELISA, the positive rate was 32.20%, in which 20.34% females and 11.86% males. (P

The effects of SLG on the immune function of normal and immune-suppressed mice were investigated. The results showed that SLG (50mg/kg, im) could correct the suppressive effect of cyclophosphamide, dexamethasone or prednisolone on spleen and. thymus weight, serum specific antibody level and lymphocyte transformation. SLG could also improve the phagocytic activity of peritoneal macrophages and enhance peripheral blood ANAE+-lymphocyte ratio of mice. No obvious effect of SLG on the normal mice were observed except that it could increase the antibody level of normal mice. The results implicate that SLG may have some immunomodulatory effect.

The regulatory effect of endothelial cells of the rabbit pulmonary arteriole(PAECs) on the tone of pulmonary artery was studied. The conditioned medium (CM) ofPAECs, cultivated under normal and hypoxic condition for 24 hours, did not induce con-striction of pulmonary artery ring. The aqueous phase was able to induce the constriction.The constriction of pulmonary artery rings was reduced by the heated aqueous phase, but it was disappeared when the heated aqueous phase was treated with trypsin. The constric-tion caused by heated aqueous phase was stronger in hypoxic endothelial cells than innormal cells. Treatment of PAECs with indomethacin and captoril, and administration ofFPL 55712, BN 52021, chlorpheniramine and prazozin to contraction pool did not affectthe constriction of pulmonary artery rings. It was concluded that PAECs might producelong half-life lipid substances which could induce the dilatation of pulmonary artery.Hypoxia inhibted the release of long half-life aqueous heat-unstable vasoconstrictive sub-stances. but enhanced that of the heat-stable fraction which was probably a peptide.

Primary biliary cirrhosis(PBC) is a chronic,progressive cholestatic liver disease of unknown cause that usually affects middle-aged women.Ursodeoxycholic acid(UDCA)is the first selected drug to PBC which has definite effect.Treament of PBC with UDCA combined with immunodepressant become presents research focuses.Liver transplantation is the only treatment of the final stage of PBC.

Objective:In occurrence and development process oflung cancer, the only known angiogenesis is one element ofthe growth, proliferation and aversion oftumor cells.VEGF, MMP-2 and MMP-9 play important role in the development and aversion ofA549 cell.This experiment is to reveal the effect ofLingqi Capsule on expression VEGF, MMP-2 and MMP-9 in A549 cell and mechanism ofanti-tumor.Methods:Medicated serum was prepared with serum-pharmacology methods, the the effect ofmedicated serum on VEGFmRNA and protein expressions ofMMP-2 and MMP-9 was detected by RT-PCR technology and immunohistochemistry respectively.Results:It suggested that the VEGF mRNAcorrect value ofthe administration group was respectively(0.804?0.020),(0.836?0.022)and(0.914?0.009),the blank control group was(0.905? 0.033)and positive control group was(0.766?0.014).Difference between the administration group and the blank control group was significant(P

The content of geniposide in Compound Zhizi Granules was determined With TLC-scanner. The optimum experiment design obtained with orthogonal test could eliminate the disturbance of other components. The result was satisfactory.