2.RNase MC2 manifests antitumor effects towards human hepatocellular carcinoma
Shuguang SU ; Xiaohua WANG ; Zhen ZHAO ; Jingfeng GUO
Chinese Journal of Clinical and Experimental Pathology 2015;(8):890-894
Purpose To determine the effect of RNase MC2 purified from momordica charantia on cell growth of hepatocellular carcino-ma ( HCC) and its underlying mechanism. Methods MTT, colony formation and nude mice model were used to examine the activity of RNase MC2 in cell proliferation. Cell cycle analysis was done by flow cytometry. Autophagy induced by RNase MC2 treatment was observed via transmission electron microscope. Western blot was performed to detect the RNase MC2-mediated changes of proteins. Re-sults In vitro and in vivo data showed that RNase MC2 markedly inhibited HCC cell proliferation, arrested cells at G2/M phase by in-creasing expression of p53 and p21, induced autophagy via upregulating Beclin-1 and LC3-Ⅱ. Furthermore, combination of RNase MC2 and Sorafenib exerted enhanced lethal effect on HCC cells. Conclusion RNase MC2 manifests significant antitumor activities and enhances the killing effect of Sorafenib in HCC via inducing cell cycle arrest and autophagy.
3.Severe disease of immune imbalance in ICU: persistent inflammation immunosuppression catabolism syndrome
Heyi SU ; Zexun MO ; Zhen CHEN ; Zhenhui GUO
Chinese Critical Care Medicine 2017;29(8):760-764
Terminology of persistent inflammation immunosuppression and catabolism syndrome (PICS) is developed based on the concept of multiple organ failure (MOF), which reflect that the preponderance is gradually reversed from pro-inflammation to anti-inflammation, and eventually the state of simultaneously persistent inflammation and severe immunosuppression appeared. Although the improvement of rescue technology and management increase the early survival rates of patient with critical illnesses, the long-term outcomes of most patients are not optimistic. The patients with PICS are difficult to treat or prevent, and are likely to indolent death and have a rising incidence, which is an important challenge to the intensive care unit (ICU). The paper review the understanding of PICS, summarize the specific changes of immune system in PICS, and explore the immunological markers for early recognition of PICS and judgment of immune state in order to provide new thinking for prevention and control of PICS.
4.Relationship between CD36 expression, foamy cell aggregates in renal interstitium and serum cholesterol level.
Hua SU ; Hong-yan ZHU ; Jian-she LIU ; An-guo DENG ; Zhen-qiong LI
Chinese Journal of Pathology 2011;40(1):42-43
CD36 Antigens
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metabolism
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Cell Aggregation
;
Cholesterol
;
blood
;
Foam Cells
;
pathology
;
Glomerulonephritis, IGA
;
blood
;
metabolism
;
pathology
;
Glomerulonephritis, Membranoproliferative
;
blood
;
metabolism
;
pathology
;
Glomerulonephritis, Membranous
;
blood
;
metabolism
;
pathology
;
Glomerulosclerosis, Focal Segmental
;
blood
;
metabolism
;
pathology
;
Humans
;
Nephritis
;
blood
;
metabolism
;
pathology
;
Nephritis, Hereditary
;
blood
;
metabolism
;
pathology
5.Concentration of target genes of SARS-CoV-2 and influencing factors in the confirmed cases with COVID-19 in Ningbo
ZHANG Dan Dan ; FU Xiao Hong ; JIAO Su Li ; GU Wen Zhen ; XU Guo Zhang
Journal of Preventive Medicine 2020;32(12):1213-1216
Objective:
To learn the concentration of target genes of severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ) and the influencing factors in the confirmed cases with coronavirus disease 2019 ( COVID-19 ) in Ningbo.
Methods:
Demographic information and clinical diagnosis of COVID-19 cases reported in Ningbo from January 21 to February 20, 2020 were collected through China Disease Control and Prevention Information System. The Ct values of ORF1ab and N of the cases were collected through Ningbo Center for Disease Control and Prevention and designated hospitals, and analyzed in terms of gender, age, severity, clinical classification, source of case, sampling time and specimen type.
Results:
There were 157 confirmed COVID-19 cases, including 56 males ( 35.67% ) and 101 females ( 64.33% ). Sixty-seven cases ( 42.68% ) aged between 40 and 60 years old. The Ct values of ORF1ab and N in females were 29.96±5.28 and 30.38±4.90, which were higher than 27.56±4.94 and 28.03±4.88 in males ( P<0.05 ). The Ct values of ORF1ab and N were the lowest when sampled at 1-7 days after onset ( P<0.05 ), which were 27.84±4.80 and 28.35±4.65. The Ct values of ORF1ab ( rs=0.288, P=0.001 ) and N ( rs=0.296, P=0.001 ) were positively correlated with sampling time. The Ct values of ORF1ab in pharyngeal swab and sputum were 29.19±4.85 and 28.74±6.40, the Ct values of N in pharyngeal swab and sputum were 29.61±4.60 and 29.22±6.10, both without significant differences between different samples ( P>0.05 ).
Conclusions
Sampling time has a great influence on the Ct values of ORF1ab and N of SARS-CoV-2.
6.The action mechanisms of Morus alba leaves extract for the treatment of diabetes based on plasma metabolomics.
Tao JI ; Li-li ZHANG ; Xiao-chen HUANG ; Shu-lan SU ; Zhen OUYANG ; Zhen-hua ZHU ; Sheng GUO ; Er-xin SHANG ; Da-wei QIAN ; Jin-ao DUAN
Acta Pharmaceutica Sinica 2015;50(7):830-835
In order to evaluate the effect and mechanism of the mulberry leaf alkaloid, flavones, and polysaccharide intervention on diabetes, the overall metabolite profiling characteristics for the plasma of diabetic mouse was performed by using an ultra-performance liquid chromatography/electrospray-tandem mass spectrometry (UPLC-ESI-MS). The 8 potential biomarkers were found in diabetic mice plasma based on the data of MS/MS characteristics obtained from the UPLC-OrbitrapMS analysis, which mainly involved in sphingolipids, amino acid metabolic pathway. The principal component analysis showed that the normal group and model group were obviously distinguished and implied that metabolic disturbance was happened in diabetic mice plasma. The extracts of mulberry leaf flavonoids, polysaccharide, alkaloid had exhibited the effects of callback function for diabetic mice through regulating the amino acid metabolism and sphingolipid metabolism.
Alkaloids
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chemistry
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Amino Acids
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metabolism
;
Animals
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Biomarkers
;
blood
;
Chromatography, High Pressure Liquid
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Diabetes Mellitus, Experimental
;
drug therapy
;
Flavones
;
chemistry
;
Flavonoids
;
chemistry
;
Metabolic Networks and Pathways
;
Metabolomics
;
Mice
;
Morus
;
chemistry
;
Plant Leaves
;
chemistry
;
Sphingolipids
;
metabolism
;
Tandem Mass Spectrometry
7.Time course of C4a and platelet aggregation rates in no-reflow patients with acute myocardial infarction undergoing percutaneous coronary intervention.
Su-xia GUO ; Zhen-yu YANG ; Ru-xing WANG
Chinese Journal of Cardiology 2011;39(2):168-170
OBJECTIVETo observe serum C4a and platelet aggregation rates changes in acute myocardial infarction (AMI) patients before and after percutaneous coronary intervention (PCI) and association with the development of no-reflow phenomenon.
METHODSFrom June 2006 to August 2009, 119 AMI patients underwent PCI (28 cases of no-reflow group, 91 cases of reflow group) and 30 subjects with suspected coronary heart diseases and normal coronary angiography results (control group) were enrolled in this study. C4a and platelet aggregation rate were measured at 30 minutes before PCI, immediately after PCI, 30 minutes, 1 hour, 2 hour, and 6 months post PCI in AMI patients and at before coronary angiography in control subjects.
RESULTSThe levels of serum C4a at 30 minutes prior to PCI in control, no-reflow, and reflow groups were similar (P > 0.05). Platelet aggregation rate at 30 minutes prior to PCI was significantly higher in no-reflow group and reflow group than in control group (all P < 0.05). Serum C4a and platelet aggregation rates were significantly higher in no-reflow group at immediate, 30 minutes and 1 hour after PCI than at 30 minutes prior to PCI, two hours and 6 months after PCI (all P < 0.05), and were significantly higher than in reflow group at immediate, 30 minutes and 1 hour after PCI (all P < 0.05). Serum C4a and platelet aggregation rates were similar at different time points in reflow group (all P > 0.05). The levels of C4a in no-reflow group at immediate, 30 minutes and 1 hour after PCI were positively correlated with platelet aggregation rates (r = 0.91, 0.79, 0.60, respectively, all P < 0.01).
CONCLUSIONThe transient increase on levels of C4a and platelet aggregation rate early post PCI are verified in no-reflow patients with AMI undergoing PCI.
Aged ; Angioplasty, Balloon, Coronary ; Case-Control Studies ; Complement C4a ; metabolism ; Humans ; Middle Aged ; Myocardial Infarction ; blood ; No-Reflow Phenomenon ; Platelet Aggregation ; Postoperative Period
9.Monocyte human leukocyte antigen-DR expression in elderly patients with sepsis.
Jun WU ; Ze LIU ; Jie SUN ; Zhen-hui GUO ; Lei SU
Journal of Southern Medical University 2008;28(9):1657-1659
OBJECTIVETo investigate the changes of human leukocyte antigen DR (HLA-DR) expression in the monocytes of elderly patients with sepsis.
METHODSA total of 213 elderly patients were divided into 4 groups according to the criteria defined by the American College of Chest Physicians/ Society of Critical Care Medicine (ACCP/SCCM), namely the non-sepsis group (group A), sepsis group (group B), severe sepsis group (group C) and septic shock group (group D). Another 60 healthy subjects were recruited as the control group. HLA-DR expression in the monocytes from each subject was determined by flow cytometry, and the HLA-DR levels were compared between the groups.
RESULTSCompared with that of the healthy control, HLA-DR expression was significantly increased in group A and lowered in group D. No significant differences were found between the control group and groups B and C. HLA-DR expression was significantly different between groups A, B, C, and D (P<0.01), increasing in the order of group D (32.74-/+13.4)%, group C (61.9-/+14.29)%, group B (69.99-/+12.8)%, and group A (85.06-/+15.37)%.
CONCLUSIONSAlthough with multiple organ diseases and repeated or long-term hospitalization, the non-septic elderly patients do not show lowered expression of HLA-DR. In the elderly patients with sepsis and severe sepsis, HLA-DR expression is not a sensitive index, but obviously lowered HLA-DR expression may serve as a specific indicator in elderly patients with septic shock.
Aged ; Aged, 80 and over ; Biomarkers ; analysis ; Female ; Flow Cytometry ; HLA-DR Antigens ; analysis ; biosynthesis ; Humans ; Leukocytes, Mononuclear ; immunology ; Male ; Sepsis ; immunology ; Shock, Septic ; immunology
10.The action mechanisms of Morus alba leaves extract for the treatment of diabetes based on plasma metabolomics.
Tao JI ; Lili ZHANG ; Xiaochen HUANG ; Shulan SU ; Zhen OUYANG ; Zhenhua ZHU ; Sheng GUO ; Erxin SHANG ; Dawei QIAN ; Jinao DUAN
Acta Pharmaceutica Sinica 2015;50(7):830-5
In order to evaluate the effect and mechanism of the mulberry leaf alkaloid, flavones, and polysaccharide intervention on diabetes, the overall metabolite profiling characteristics for the plasma of diabetic mouse was performed by using an ultra-performance liquid chromatography/electrospray-tandem mass spectrometry (UPLC-ESI-MS). The 8 potential biomarkers were found in diabetic mice plasma based on the data of MS/MS characteristics obtained from the UPLC-OrbitrapMS analysis, which mainly involved in sphingolipids, amino acid metabolic pathway. The principal component analysis showed that the normal group and model group were obviously distinguished and implied that metabolic disturbance was happened in diabetic mice plasma. The extracts of mulberry leaf flavonoids, polysaccharide, alkaloid had exhibited the effects of callback function for diabetic mice through regulating the amino acid metabolism and sphingolipid metabolism.