1.Experimental Study on Treating Cholecystitis on Guinea Pigs by Jinyin Granule.
Yuchun WANG ; Fuqin SU ; Shu MIAO ; Li ZHOU ; Tao LI
Journal of Medical Research 2006;0(09):-
Objective To investigate the action and the mechanism of Jinyin granule(JYG) on cholecystitis in guinea pigs.Methods Cholecystitis models of Guinea pigs were established and then randomly divided into six groups: normal group,model group,XiaoYanLiDan tablet(XYLDT) group,treatment groups including low,middle and high dosage groups of JYG.The Pigs were given above drugs respectively while in normal and model groups the same volume of normal saline was given.Body weight,gallbladder weight,bile amount,IL-1? in the serum were measured and gallbladder pathological tissue was observed by light microscopy.Results Comparing with the model group,the treatment groups had higher body weight,lower gallbladder weight,less bile amount,obviously slighter inflammation and less amount of IL-1? in the serum.Conclusion JYG possesses the therapeutic action to cholecystitis on guinea pigs and its mechanism is interrelated to the decreasing of IL-1? in the serum.
3.Brief introduction of acupuncture needling and teaching keypoint.
Shu-wei HOU ; Li GUO ; Su-ping KONG
Chinese Acupuncture & Moxibustion 2014;34(9):915-918
We summarized our accumulated clinical and teaching experiences and explored the regularity of acupuncture needling and teaching. It is of great importance in pressing hand during inserting needle. Stroking and pressing are two crucial parts which deserve more attention, and seldom useage of pressing hand should be abolished. Operating hand needs practice before inserting needle, while it should fully relaxed during inserting. Blending "touching", "stretch" "gathering" "erupting" and "advancing" in single moment, applying appropriate dynamic mode of inserting needle such as "join 3 forces as one" "3 points in a line" expertly and naturally. In addition, enough attention should be paid on "altering direction" and "shifting point". Inserting deftly and powerfully, no/slight sensation, deqi when inserting needle are the highest reflection as an acupuncturist.
Acupuncture
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education
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Acupuncture Therapy
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instrumentation
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methods
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China
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Humans
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Teaching
4.Effects of thalidomide on the expression and secretion of vascular endothelial growth factor and tumor necrosis factor-alpha by a human keratinocyte cell line HaCaT
Fei SU ; Hongzhong JIN ; Feng LI ; Dan SHU
Chinese Journal of Dermatology 2013;46(7):492-495
Objective To investigate the effect of thalidomide on the proliferation of as well as the expression and secretion of vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-α) by the human keratinocyte cell line HaCaT.Methods Cultured HaCaT cells were divided into several groups to be treated with dimethyl sulfoxide (negative control group) and various concentrations (0.01nmol/L-100 μmol/L) of thalidomide (experimental groups) respectively for 20 to 24 hours.Subsequently,water soluble tetrazolium-1(WST-1) assay was performed to estimate cellular proliferation,real time quantitative PCR to detect the mRNA expression of VEGF and TNF-α in HaCaT cells,and enzyme-linked immunosorbent assay (ELISA) to quantify the protein expressions of VEGF and TNF-α in the culture supernatants of HaCaT cells.Statistical analysis was done by one-way analysis of variance with least significant difference post hoc test.Results The survival rate of HaCaT cells was 74.3%,82.9% and 90.8% after 24-hour treatment with thalidomide of 100,10 and 1 μmol/L respectively,significantly lower than that in the negative control group (100%,all P <0.01).A significant decrease was induced in the mRNA expression (0.439-to 0.634-fold change,all P <0.01) and supematant level ((0.587-to 0.923-fold change,P <0.05) of VEGF in HaCaT cells by thalidomide of 0.01-100 nmol/L,as well as in the mRNA expression (0.493-to 0.587-fold change,P <0.05) and supernatant level (0.408-to 0.617-fold change,P <0.01) of TNF-α by thalidomide of 0.1-100 nmol/L.Conclusion Within a certain range of concentration,thalidomide could suppress the proliferation of,as well as the expression and secretion of VEGF and TNF-α by,HaCaT cells.
5.Serum levels of interleukin-36 and its receptor antagonist in patients with psoriasis and their correlations with disease severity
Dan SHU ; Hongzhong JIN ; Baoxi WANG ; Fei SU ; Feng LI
Chinese Journal of Dermatology 2014;47(7):469-472
Objective To evaluate the relationship of serum levels of interleukin (IL)-36α,IL-36β and IL-36γas well as their receptor antagonist IL-36Ra with disease severity in patients with psoriasis.Methods Venous blood samples were collected from 45 patients with generalized pustular psoriasis (GPP),34 patients with psoriasis vulgaris (PV),and 37 healthy human controls.Enzyme-linked immunosorbent assay (ELISA) was performed to determine the serum levels of IL-36α,IL-36β,IL-36γ and IL-36Ra.Nonparametric Mann-Whitney test was conducted to compare the levels of IL-36 and IL-36Ra among these groups,and Spearman's rank correlation analysis to assess the relationship between the serum levels of IL-36 and IL-36Ra and disease severity.Results No statistical difference was observed in the serum levels of IL-36 or IL-36Ra among the patients with GPP,patients with PV,and healthy human controls.The serum levels of IL-36β and IL-36γ (given as the median ± interquartile range) were significantly higher in 27 patients with GPP during episodes of pustules ((12.101 ± 11.315) ng/L and (34.541 ± 15.580) ng/L respectively) and in 7 patients with severe GPP ((11.218 ± 9.318) ng/L and (38.536 ± 17.332) ng/L respectively) than in the healthy human controls ((5.355 ± 9.020) ng/L and (23.052 ± 22.410) ng/L respectively,P < 0.05 or 0.01).The serum level of IL-36γwas positively correlated with that of IL-36β in patients with GPP,patients with PV,and the healthy human controls (r =0.85,0.86,0.91,respectively,all P < 0.01),and both IL-36β and IL-36γserum levels were lowly and positively correlated with the severity of GPP (r =0.33,0.41,respectively,both P < 0.05).A positive correlation was also observed between the serum level of IL-36β and psoriasis area and severity index (PASI) scores in patients with PV (r =0.54,P < 0.01).Conclusions The serum levels of IL-363 and IL-36γ are lowly and positively correlated with disease severity in patients with GPP,suggesting that IL-36β and IL-36γplay an important role in the pathogenesis of GPP.
6.Changes of Electrocardiogram after Acute Stroke
Li LI ; Huairong LIU ; Junlian SHU ; Ping WEI ; Yan WANG ; Jinhua SU
Chinese Journal of Rehabilitation Theory and Practice 2007;13(6):550-551
Objective To investigate the feature and mechanism of electrocardiogram (ECG) changes in different onset parts after different types of acute stroke.MethodsA retrospective analysis about ECG was performed according to data of 163 acute stroke patients.ResultsThe abnormal ratio of ECG had no significant difference between hemorrhagic stroke and ischemic stroke. Moreover, the patients with onset areas on basal ganglia and thalamus had a higher rate of abnormal ECG.ConclusionThe patients with acute stroke have a higher incidence of abnormal ECG. The changes of ECG are associate with types and areas of injury.
7.Effect of Qishen Huoxue Granule in treating severe sepsis.
Yan-Li SU ; Hong WANG ; Shu-Wen ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(3):209-211
OBJECTIVETo study the anti-inflammatory effect and the therapeutic efficacy of Qishen Huoxue Granule (QHG) in treating severe sepsis.
METHODSOne hundred and sixty-seven patients with severe sepsis were enrolled and randomly assigned to two groups, the 85 patients in the control group treated with conventional Western medicine and the 82 in the QHG group treated with conventional Western medicine plus QHG. Changes of tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-10 (IL-10), procalcitonin (PCT), Marshall score, APACHE II score, ICU stay time and 28-day mortality were monitored and compared.
RESULTSCompared with the control group, IL-6 and TNF-alpha levels, the ICU stay time and 28-day mortality were significantly lower in the QHG group (all P < 0.05). During the QHG treatment, no severe adverse event was observed.
CONCLUSIONThe integrative treatment could reduce the blood levels of IL-6 and TNF-alpha, shorten the ICU stay time and decrease the 28-day mortality of patients with sepsis, showing a favor therapeutic prospect.
Aged ; Aged, 80 and over ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Interleukin-6 ; blood ; Male ; Middle Aged ; Phytotherapy ; Sepsis ; blood ; drug therapy ; pathology ; Treatment Outcome ; Tumor Necrosis Factor-alpha ; blood
8.Siwu series decoctions for treating primary dysmenorrea of gynecology blood stasis syndrome--research progress of Taohong Siwu decoction.
Li LIU ; Jin-ao DUAN ; Shu-lan SU ; Pei LIU ; Yu-ping TANG ; Da-wei QIAN
China Journal of Chinese Materia Medica 2015;40(5):814-821
The relevant literatures of Siwu series decoctions--Taohong Siwu decoction for treating primary dysmenorrea of gynecology blood stasis syndrome were summarized including the clinical application, pharmacological effects, bioactive components and metabolic characteristics. On this basis,research on the bioactive components and formulation rule were discussed, to provide reference for modern traditional Chinese medicine formula study.
Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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therapeutic use
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Dysmenorrhea
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drug therapy
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Female
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Humans
9.Analysis of the management staff and funds allocation in the new rural cooperative medical system in Guangxi
Yanli ZUO ; Sheng WANG ; Yusha GUO ; Fang SU ; Caiyuan WU ; Shu LI
Chinese Journal of Hospital Administration 2014;30(8):629-631
Objective To learn the management staff and funds allocation of the NRCMS in Guangxi,and come up with solution accordingly.Methods Collection of the annual report data of NRCMS in Guangxi from 2009 to 2012,and calculation of the management personnel and funds expenditure.Results The management personnel of NRCMS in Guangxi is found with a 1000-person gap between the actual staffing and approved staffing quota; the management funds rise year by year which come mostly from fiscal appropriation.Yet it accounts for less than 2.00% and with a high rate of surplus; per-capita funds for management personnel rise significantly,along with their per-capital salaries,yet the highest fall below 25000 yuan per year per person.Conclusion Staffing quota should be fixed more rationally to ensure the number and competence of NRCMS management staff; more funds and better use of management funds are required.
10.Expression of musca domestica β-glucosidase in the organs besides digestive system of Ⅲ instar larvae
Rong HU ; Shu ZHANG ; Yue HUANG ; Min SU ; Hong LI ; Guo GUO ; Ping FU ; Jianwei WU
Journal of Regional Anatomy and Operative Surgery 2016;25(7):473-477
Objective To study whether the organs besides digestive system of musca domestica Ⅲ instar larvae have the capability of produceing musca β-glucosidase.Methods Tissues of malpighian tubules,trachea,epiploon and body wall of musca domestica Ⅲ instar lar-vae were dissected under anatomic microscope,and the expression of β-glucosidase gene in these dissected tissues were detected by reverse transcription PCR.And the tissue localization of β-glucosidase mRNA was further identified by in situ hybridization.Moreover,anti-cellulase was used to determinate the tissue distribution with immunohistochemical staining.The relative mRNA expression levels of musca domesticaβ-glucosidase gene in these organs were tested by real-time quantitative PCR.Results The reverse transcription PCR showed that the ampli-fication products of β-glucosidase gene were observed in tissues of malpighian tubules,trachea and body wall.β-glucosidase mRNA was shown in the epithelium cells of malpighian tubules,trachea and body wall by in situ hybridization,and it was almost the same in the results of im-munohistochemical staining.The real-time quantitative PCR showed that the relative expression quantity of β-glucosidase gene in malpighian tubules and body wall were higher than that in foregut,while it was lower in itrachea than that in foregut.And it was of statistical difference in gene expression level of β-glucosidase among these organs (P <0.05).Conclusion Malpighian tubules,trachea and body wall of musca domestica Ⅲ instar larvae have the function of secreting β-glucosidase.Combining with the characteristics of secreting β-glucosidase in most organs of digestive system,it may provide a new biological method for the prevention and treatment of human diseases transmitted by musca domestica with the use of taget gene β-glucosidase.