Objective To explore changes of risk factors between premenopausal and postmenopausal female patients with coronary heart disease (CHD). Methods A total of 283 female patients were divided into CHD group (n=154) and con?trol group (n=129) according to whether they suffered from CHD or not. Patients were further stratified into two groups accord?ing to menopausal situation:premenopausal group (n=78, including premenopausal CHD group of 34 cases and premenopaus?al control group of 44 cases) and postmenopausal group (n=205, including postmenopausal CHD group of 120 cases and post?menopausal control group of 85 cases). Clinical data of age, body mass index (BMI), medical history and menses situation were recorded. The levels of cholesterol (CHO), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipo?protein cholesterol (LDL-C), very low density lipoprotein cholesterol (vLDL-C), apolipoprotein AI (ApoAI), apolipoprotein B (apoB), lipoprotein a (Lpa), homocysteine (HCY) and high-sensitive C-reactive protein (hsCRP) were also detected. Results There were significantly higher levels of age, CHO, TG, LDL-C, vLDL-C, Lpa, ApoB, hsCRP, and the ratio of hypertension history, diabetic history, coronary heart disease family history in CHD group than those of control group. The level of HDLc was significantly lower in CHD group than that of control group. The ratio of hypertension history, diabetic history and levels of CHO, TG, LDL-C, vLDL-C, Lpa, ApoB, hsCRP were significantly higher in both premenopausal and postmenopausal CHD groups than those of their control groups. The level of HDL-C was significantly lower in postmenopausal CHD group than that of postmenopausal control group (P<0.05). Hypertension history, diabetic history and LDL-C were the independent risk fac?tors of premenopausal CHD. Hypertension history, diabetic history and CHO were the independent risk factors of postmeno?pausal CHD, and HDL-C was the protecting factor of CHD. The ratio of hypertension history, the level of CHO and LDL-C were significantly higher in postmenopausal CHD group than those of premonopausal CHD group. Conclusion The abnor?mal blood pressure level and dyslipidemia are more prevalent in postmenopausal female patients with CHD than premeno?pausal female patients. We should control these risk factors to improve the prognosis.

AIM: This experiment was performed to evalu at e the significance of the expression of different types of protein kinase C (PKC ) in lung squamous cell carcinoma induced by methylcholanthrene(MAC).METHODS: Lung squamous cell carcinoma in Wistar rats was induced with MAC for 12 weeks. The stage of the cancer development was determined histop athologically. Immunohistochemistry method was used to study the expression of different types of protein kinase C ?,?,?,? in lung squamous cell carcinoma. RESULTS: The level of expression of PKC ? in lung squamous cel l car cinoma was significantly higher than that in peripheral lung tissues and normal lung tissues (24.08%?2.93%,5.00%?2.10%,1.96%?0.29%,respectively. P

Literature of TCM contains abundant information of Chinese medicine. Traditional Chinese medicine information is characterized by its high degree of knowledge-intensive and complex inter-knowledge, so it is difficult to integrate and share knowledge. TheShanghanlun is one of the Chinese medical classics, and it includes the rules and methods of syndrome differentiation and treatment and it showed good effects in the clinical application. However, it is difficult to master for beginners. As a kind of knowledge organization tool, topic maps can flexibly define the relationship between knowledge, which realizes the visualization of knowledge network, and integrates and shares the distributed knowledge. This paper focused on the current situation of topic maps researches. The topic maps was used to organize the original content ofShanghanlun, construct the concept model of it and explore the specific application of topic maps technology in the field of traditional Chinese medicine.

Objectives:The effects of parenteral and enteral nutrition combined Octreotide on pancreatic secretion of patients with pancreatic fistula were compared. Methods:Pancreatic juice,drained directly from the pancreatic fistula,was collected before and after using Octreotide,and volume,bicarbonate,protein,amylase,HCO - 3,K +,Na + and Cl - were measured. Results:No differences in exocrine pancreatic secretion were observed between the enteral and parenteral methods of feeding.There were significant decreases in pancreatic secretion volume,bicarbonate,protein,amylase,HCO - 3,K +,Na + and Cl - after using Octreotide( P

Objective To develop an HPLC method for the determination of mycophenolic acid(MPA), mycophenolic acid glucuronide(MPAG) in plasma.Methods The samples were precipitated with zinc sulphate-methanol solution before injection.Carbamazepine was selected as internal standard,ZORBAX XDB C18 (4.6 mm ×250 mm,5 μm) column was used and the flow rate was 1 mL/min.The mobile phase consisted of methanol-acetonitrile-potassium dihydrogen phosphate buffer solution(gradient elution) .The column temperature was 30℃ and the detective wave length was 254 nm.And then the MPA,MPAG concentration of 32 patients in 7-14 days after renal transplantion were determined.Results The assay was linear within 0.2-50μg/mL for MPA, 2.5-500 μg/mL for MPAG(r>0.999).Absolute recovery rates of MPA,MPAG were more than 80%, the recoveries were between 90%-110%.The intra-day and inter-day RSDs were both lower than 10%.Totally 32 cases of renal transplantion patiens were with mycophenolate mofetil at the dose of 1-1.5 g/d,and MPA in plasma was within the range of 0.32-6.19μg/mL,MPAG in plasma was within the range of 9.52-149.25μg/mL.Conclusion The method is accurate, convenient and rapid, which could be used in the quantitative determination of plasma concentration of MPA,MPAG in renal transplantion patients.

0.05]. At 20, 30 and 40 days concentration of endostatin[(212.80?85.91) ?g/L,(293.63?62.53) ?g/L, (271.57?32.45) ?g/L, respectively] were higher than that of the control group (P

OBJECTIVEThis study aims to detect the expression level of interleukin-23 (IL-23) in tongue squamous cell carcinoma tissues and its relationship with clinical prognosis, as well as explore the anti-apoptotic and drug resistance of the tongue squamous cell line-SCC9 before and after treatment with IL-23.

METHODSThe expression of IL-23 in tumor tissues from 28 tongue cancer patients was analyzed by immunohistochemistry assay. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the mRNA expression of Wingless-related integration site (Wnt)1 and c-myc in SCC9 cells treated with different IL-23 concentrations. After interferencing the β-catenin with small interfering RNA (siRNA), the expression of β-catenin, B-cell lymphoma-2 (Bcl-2), ATP-binding cassette sub-family G member 2 (ABCG2), and permeability-glycoprotein (P-gp) in SCC9 was measured by Western blot analysis. The effect of IL-23 on the apoptotic resistance of SCC9 to cisplatin was examined by methyl thiazolyl tetrazolium test.

RESULTSThe expression of IL-23 in tongue cancer tissues was correlated with lymphatic metastasis, nerve invasion, and the recurrence after therapy (P<0.05). After dealing with IL-23, SCC9 showed the upregulation effect of Bcl-2, ABCG2 and P-gp expressions. IL-23 was closely related to the activation level of the Wnt pathway and significantly strengthened the resistance to cisplatin (P<0.01).

CONCLUSIONIL-23 activates the Wnt pathway in tongue squamous cell carcinoma, thereby enhancing its resistance to apoptosis and drug.

Apoptosis ; Carcinoma, Squamous Cell ; metabolism ; Cell Line, Tumor ; Cisplatin ; Drug Resistance, Neoplasm ; physiology ; Humans ; Interleukin-23 ; metabolism ; Interleukin-23 Subunit p19 ; Lymphatic Metastasis ; Neoplasm Recurrence, Local ; RNA, Small Interfering ; Real-Time Polymerase Chain Reaction ; Tongue Neoplasms ; metabolism ; beta Catenin ; metabolism

OBJECTIVETo dicuss the application and therapeutic effect of middle-forearm flap based on perforator of ulnar artery for electrical burn wound on the wrist.

METHODSFrom Oct. 2009 to Oct. 2012, 10 cases of electrical burn wounds on the wrist were treated. A line from radialis medial epicondyle of humerus to the interior radialis pisiform bone was connected as flap axis. At the midpoint of the line, Doppler flow imaging meter was used to detect the emerging point of perforator vessel. The flap was designed and harvested. The flap was transferred reversely, with superficial vein retaining which was anastomosed with vein at recipient sites in 3 cases. The wounds in the donor sites were closed directly in 2 cases, and with skin graft in 8 cases.

RESULTSAll the 10 flaps survived completely. 7 cases without vein anastomosis underwent obvious flap edema during 2-4 days postoperatively, which resovled 1 week later. Sub-flap tissue necrosis and infection happened in 2 cases, which healed after dressing and drainage. Patients were followed up for 3-36 months with satisfactory results.

CONCLUSIONSThe middle-forearm flap based on perforator of ulnar artery has a stable and reliable blood supply. It offers a new choice for the electric burn wound on the wrist, especially at the ulnar side.

Burns, Electric ; surgery ; Forearm ; Humans ; Reconstructive Surgical Procedures ; Skin Transplantation ; Surgical Flaps ; blood supply ; transplantation ; Ulnar Artery ; Wrist Injuries ; surgery

Amyloid ; metabolism ; Humans ; Male ; Middle Aged ; Pituitary Neoplasms ; metabolism ; pathology ; surgery ; Plaque, Amyloid ; pathology ; Prolactin ; secretion ; Prolactinoma ; metabolism ; pathology ; surgery ; Synaptophysin ; metabolism

Objective To determine the prevalence of and correlations between HIV infection and syphilis among men who have sex with men (MSM) in Beijing.Methods Totally,600 MSM collected from sentinel surveillance sites for AIDS in Beijing were recruited in this study,and underwent screening tests for HIV infection and syphilis.Enzyme-linked immunosorbent assay (ELISA) was used for the primary screening for HIV infection,and Western blot for its confirmation.Both nontreponemal test (rapid plasma regain (RPR) assay) and treponemal test (Treponema pallidum particle agglutination assay,TPPA) were performed to detect syphilis,and only those with a reactive TPPA result were defined as having syphilis (including past infection).Specimens confirmed as HIV-1 positive were further tested with BED-capture enzyme immunoassay (CEIA) to find recent HIV-1 infection,and the incidence of HIV infection was then calculated.The correlation between syphilis and HIV infection was statistically analyzed.Results Of the 600 MSM,49 (8.17％) had HIV infection,including 14 cases of recent infection,with the incidence rate of HIV infection being 4.68％; 51 were positive for both RPR and TPPA tests with the infection rate (including past infection) being 18.5％ (111/600).People without a Beijing household registration and those with high education levels accounted for a large proportion of HIV-infected MSM.Conclusions The prevalence and incidence of HIV infection as well as prevalence of syphilis maintain at a relatively high level in MSM in Beijing.HIV infection is markedly correlated with syphilis in MSM population.