0 55). The Vmax and Vmin of the patients were singnificantly higher than those of healthy people. The RI of healthy subjects was higher than that of the patients. The Vmax and Vmin of the kidney with tumor was singnificantly higher than that of the other normal kidney side. Conclusion The hemodynamic features of the kidney tumors and kidney artery were valuable to the differential diagnosis of the malignant and benign kidney tumors.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Digestive System Neoplasms ; complications ; surgery ; Female ; Humans ; Intestinal Obstruction ; etiology ; therapy ; Male ; Middle Aged ; Postoperative Complications ; therapy ; Young Adult

Objective To investigate 60Co γ-ray induced damage in lymphocytes and the relationship between doses of 60Co γ-ray irradiation and the levels of phosphorylated H2AX and ATM.Methods Cells were irradiated with 60Co γ-rays in the range of 0-8 Gy.The levels of phosphorylated H2AX and ATM were detected by Western blot and FACScan,respectively.The micronucleus(MN)was analyzed by CB method to evaluate DNA damage.Results FACScan results showed the dose-effect relationship of γ-H2AX expression were linear.square at 0.5 h post-irradiation to different doses,and the fitting curve was shown as Y=3.96+11.29D-0.45D2.The level of phosphorylated ATM(p-ATM)was not changed significantly by using the same method.Western blot showed that p-ATM protein expression was significandy increased after irradiation compared with sham.irradiated group.The MN assay which represented DNA damage was sensitive to different doses.Conclusions γ-ray irradiation could induce the phosphorylation of H2AX and ATM,which may play an important role in indicating DNA damage.Both of H2AX and ATM have the potential as sensitive biomarker and biodosimeter for radiation damage.

Objective To investigate the effects of 60Co γ-ray partial radiation on chromosome aberration in human peripheral blood in vitro.Methods The samples of heparinized peripheral whole blood from 3 healthy persons were exposed to 60Co γ-rays at the doses between 0 and 8 Gy with the dose rate of 0.35 Gy/min at the temperature of 37 ℃ ,and then mixed with the unirradiated blood samples of the Microscopy was used to observe the chromosome aberration double ( centromere + centromere) and the biological dose was estimated thereby.ResultsThe amounts of double centromere + centromere were increased along with the dose of irradiation in all groups.The estimated biological dose was higher than the 1/3 of the irradiation dose when the dose was between 0.5 to 2 Gy,and was close to the 1/3 of the irradiation dose when the dose was between 4 to 8 Gy.Conclusion Chromosome aberration can be used as a biomarker in estimation of uneven irradiation.

Objective To analyze the chromosome aberrations induced by partial radiation in human peripheral blood lymphocytes in vitro.Methods Heparinized whole blood samples were exposed to 2 Gy ~(60)Co 7-rays at 37℃ ,and then mixed with non-irradiated blood by different ratio.The slides were prepared after culturing and the unstable aberrations were analyzed.Results The chromosome aberrations had a good relationship with the ratio of irradiated blood.The chromosome aberrations in partial irradiated group were higher than that in the irradiated group.The estimated dose was 1.27 Gy when the ratio was 1 : 1 ,greater than the dose of 1 Gy.The estimated dose was 0.93 Gy when the ratio was 0.5＝1,also greater than 0.5 Gy.But when the ratio was 1:0,the radiation dose was accordant with the estimated dose.Conclusions Chromosome aberrations could be a biomarker for estimating the uneven irradiation.

Objective To explore the effect of radon and its progeny on the expression and mutations of p53 in lung tissue of mouse model. Methods Apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP-biotin nick end labeling. The expression of p53 gene was analyzed by immunohistochemistry, Western blot and realtime-PCR. PCR-SSCP was used to detect the mutation of p53 in lung tissues. Results Compared with those in the control group, the apoptotic index were increased significantly in 30 WLM and 60 WLM groups( t = 18.11, -10.30,P ＜ 0.05 ). The p53 protein was increased significantly ( t = -11.08, P ＜ 0.05; t = - 7.00, P ＜ 0.0 ) ) in 30 WLM and 60 WLM groups. The mutation of p53 gene was not detected in lungs of radon-exposure mice. Conclusions Lung and bronchus might be the targets of radon and its progeny, and p53 gene plays an important role in the progression of radon-induced lung injury.

Objective To evaluate the teaching effects of micro-lecture based on the wechat platform in new nurses'training in operating rooms.Methods Fifteen new nurses in the operating room in July 2014 were selected as observation group,and thirteen new nurses in July 2013 were selected as control group.The multimedia-aided teaching mode was applied in the two groups,while micro-lecture based on the we-chat platform was implemented in the observation group.The theory and operating skills between two groups were evaluated by examination, and a random inquiry about satisfaction with new nurses was directed on surgeon and seniors nurses.Resulsts 100% of the nurses in obser-vation group accepted and preferred wechat in continuing education.The new nurses’performance in theory and skill test in the observation group was significantly higher than that of the control group (t =2.901,P =0.011;t =2.225,P =0.029).In addition,the surgeon’s and seniors nurses’satisfaction with new nurses in the observation group was significantly higher than that of the control group (Z =6.425,P =0.011).Conclusion Micro-lecture based on the wechat platform can motivate nurses’learning enthusiasm and positivity,and effectively improve their theoretical knowledge and professional skills.

Clinical characteristics were retrospectively analyzed in a patient with parathyroid crisis as the main symptoms of parathyroid adenoma and asymptomatic pheochromocytoma.This analysis was aimed to implement specific diagnosis and treatment and to accumulate experience in managing these diseases.

Objective To evaluate the effect of AIDS health education in university students to provide the theoretical basis for AIDS prevention in the young college students .Methods In order to know about the situation of awareness HIV/AIDS ,a general survey of HIV/AIDS related knowledge was conducted in freshmen of Dali college by self‐made questionnaire In December 2010 , then various forms of health education was took for college students ,such as theoretical study ,lecture and so on .Investigation a‐mong students once again ,and compared with the baseline survey results in December 2012 .Results The accuracy of HIV/AIDS health knowledge in the young college students after had health education was higher significantly than that before(P<0 .05);The score of AIDS elated problem have been weighted ,and awareness was defined as≥60 ;Before health education ,2 619 students knew about AIDS related knowledge ,872 students did not ,the average score was (64 .50 ± 10 .22) ,the total awareness rate was 75 .67% ;after the health education ,3 323 students know about AIDS related knowledge ,138 students did not ,the average score was (84 .94 ± 12 .68) ,the total awareness rate was 96 .01% .After continuing health education ,the total score was significantly increased (P<0 .01) .Conclusion The awareness rate of AIDS could be improved by health education in various forms and that was impor‐tant for the prevention of AIDS among college students .

Objective To identify the differentially expressed microRNAs in the early transformed cells,the late transformed cells and their parental BEP2D cells.Methods The differentially expressed microRNAs in the above cells were identified by microRNAs microarray assay.Results There were 38differentially expressed microRNAs in R15H20 cells versus BEP2D cells,with 18 upregulated and 20downregulated microRNAs.R15H20 and RHT35 cells shared 25 differentially expressed microRNAs compared with BEP2D cells,with 15 down-regulated and 10 up-regulated microRNAs.There were 87differentially expressed microRNAs in RHT35 cells versus BEP2D cells,with 47 upregulated and 40 downregulated microRNAs.There were 38 differentially expressed microRNAs in RHT35 cells versus R15H20 cells with 20 upregulated and 18 downregulated microRNAs.Conclusions microRNAs are differentially expressed in the different stages of carcinogenesis of BEP2D cells induced by α particles,which suggests that microRNAs may play an important role in α particle-induced malignant transformation of BEP2D cells.