Objective To study the cautious behaviors of mice under dangerous conditions caused by height. Methods An elevated beard task was designed,in which the mice were employed to get through a horizontally elevated beard. The time of getting through and number of looking-aside behaviors were recorded under different experimental conditions with different elevated height and visual cues. Results The mean time of getting through the elevated board with a height of 60cm was significantly longer than that of 15cm [(22.55±14.64)s Vs (12.16±8.67)s, P<0.05]. The number of looking-aside behaviors decreased from (2.95±1.73) to (1.95± 1.27) , P <0.05 ,when the visual cues on potential danger were controlled. Conclusion The cautious behaviors increase with the height of elevated board and decrease when controlling for the visual cues on potential danger, which indicate a trade-off relationship between the perceived danger and cautious behaviors.

Objective: To analyze the association of type 2 diabetes mellitus (DM) with the clinical and coronary angiographic features of coronary heart disease (CHD) in patients aged over 70 years old. Methods: A total of 310 elderly patients with coronary angiograph-confirmed coronary diseases, who were treated in Changhai Hospital during Apr. 2006 to Jul. 2008, were retrospectively analyzed. The patients were further divided into 2 subgroups according to the presence of DM: DM-CHD group (n = 155) and non-DM-CHD group (n = 155). The age, gender, blood pressure, blood lipid, ejection fraction (EF), the angiographic outcomes, etc. were analyzed and compared between the two groups. Results: The incidence of hypertension was significantly higher in the DM-CHD group than in the non-DM-CHD group (P<0.05). The fasting blood glucose (FBG), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and fibrinogen (FIB) were significantly higher than those in the other group (P<0.01), and the high density lipoprotein cholesterol (HDL-C) was lower than that in the other group (P<0.05). The EF value in the DM-CHD group was significantly lower than that in the non-DM-CHD group (P< 0.01). Among the 35 (22%) patients preliminarily diagnosed as having DM, 14 (40%) would be misdiagnosed if the diagnosis depends solely on FBG without oral glucose tolerance test (OGTT). The prevalence of diffusive coronary lesions in the DM-CHD group was significantly higher than that in the non DM group (P<0.01). DM-CHD group also had significantly higher coronary stenosis index (P<0.01) and more occlusive vessels than non-DM-CHD group. Conclusion: The missed diagnosis rate of DM is high in CHD patients. Compared with non-DM-CHD patients, DM-CHD patients are at higher risks for coronary disease and have more severe coronary lesions.

Objective: To evaluate the clinical effect of combined acupuncture and electrophoresis in treating trigeminal neuralgia. Methods: Sixty patients were randomly allocated to two groups. The treatment group received acupuncture plus electrophoresis, and the control group took Carbamazepine and Methylcobalamin. The therapeutic effects would be evaluated after 30 d. Results: The total effective rate was 93.3% in the treatment group versus 70.0% in the control group. During the follow-up study (6 months to 1 year), 3 cases got relapsed in the treatment group, occupying 11.1%, and 6 got relapsed in the control group, occupying 28.6%. Conclusion: Combined acupuncture and electrophoresis has a precise effect in treating trigeminal neuralgia, and it bears an advantage of simple operation and maintaining cutaneous sensation.

Objective: To preliminarily investigate the relationship between the baseline level of serum soluble ST2 (sST2) and 30-day MACE occurrence rate in patients with ST-elevation myocardial infarction (STEMI).
Methods: A total of 121 patients with confirmed diagnosis of STEMI in our hospital from 2015-05-01 to 2015-07-30 were consecutively enrolled. According to baseline sST2 level, the patients were divided into 2 groups:Low sST2 group, the patients with sST2≤56.68 ng/ml, n=61 and High sST2 group, the patients with sST2>56.68 ng/ml, n=60. Clinical condition and 30-day MACE (defined as death and new onset of congestive heart failure) occurrence rate were compared between 2 groups.
Results: ① The systolic blood pressure (SBP), Killip class≥II grade, blood levels of cTNI, NT-proBNP, hs-CRP and LVEF were different between 2 groups, all P<0.05. ② Baseline sST2 level was positively related to heart rate (r=0.271, P=0.003), Hs-CRP (r=0.359, P=0.000), cTNI (r=0.324, P=0.000) and NT-proBNP (r=0.425, P=0.000);negatively related to SBP (r=-0.226, P=0.013) and LVEF (r=-0.406, P=0.000).③30-day MACE occurrence rate was different between 2 groups (8.2%vs 30%, P=0.002). ④ Multivariate Cox regression analysis presented that sST2>56.68 ng/ml was the risk factor for 30-day MACE occurrence (HR=1.152, 95%CI 1.078-1.231, P=0.000).
Conclusion: Increased baseline level of sST2 implied the higher incidence of death and new onset of congestive heart failure in STEMI patients.

Objective:To investigate the training needs of nursing students during their practice in the emergency department, and to provide evidence for further improvement and development of the training.Methods:A total of 279 nursing students from 24 hospitals in Guangdong Province and 4 undergraduate universities running nursing specialty were investigated by convenience sampling. The contents of the questionnaire included general information and emergency training needs, including teaching contents and teaching methods.Results:Among the surveyed nursing students, 97.13% (271 students) believed it was very necessary to learn first aid knowledge and skills, and 85.66% (239 students) were very interested in learning first aid knowledge and skills. The score of "acute and critical skills" in the emergency training needs was the highest (88.91 points), and the score of "department rules and regulations" was the lowest (76.08 points). Univariate analysis showed that there were significant differences in gender, willingness to engage in nursing work in the future, interest in learning first aid knowledge and willingness to learn first aid knowledge independently by network ( P < 0.05). The three teaching methods ranked the highest in the survey of emergency teaching methods were situational teaching, problem-based learning and action teaching method. Conclusion:Nursing students have a strong demand for training during the emergency internship. Clinical nursing administrators and educators should combine the internship demand of nursing students and the teaching outline of medical colleges to carry out the training arrangement during the emergency practice, so as to meet the learning needs of nursing students during the emergency practice.

To investigate cytotoxic secondary metabolites of Micrococcus sp. R21, an actinomycete isolated from a deep-sea sediment (-6 310 m; 142 degrees 19. 9' E, 10 degrees 54. 6' N) of the Western Pacific Ocean, column chromatography was introduced over silica gel, ODS, and Sephadex LH-20. As a result, eight compounds were obtained. By mainly detailed analysis of the NMR data, their structures were elucidated as cyclo(4-hydroxy-L-Pro-L-leu) (1), cyclo(L-Pro-L-Gly) (2), cyclo( L-Pro-L-Ala) (3), cyclo( D-Pro-L-Leu) (4), N-β-acetyltryptamine (5), 2-hydroxybenzoic acid (6), and phenylacetic acid (7). Compound 1 exhibited weak cytotoxic activity against RAW264. 7 cells with IC50 value of 9.1 μmol x L(-1).
Animals ; Biological Factors ; chemistry ; isolation & purification ; metabolism ; pharmacology ; Cell Survival ; drug effects ; Macrophages ; cytology ; drug effects ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Mice ; Micrococcus ; chemistry ; genetics ; isolation & purification ; metabolism ; Molecular Structure ; Phylogeny ; RAW 264.7 Cells ; Seawater ; microbiology ; Secondary Metabolism

Objective To investigate the effect of signal transducers and activators of transcription 3(STAT-3)on sen-sitizing oral squamous cell carcinoma to cis-dichlorodiamineplatinum via downregulating miRNA-21. Methods Tscca and Tca8113P160 human tongue squamous cell carcinoma cell lines were employed in this study. WP1066 was used to suppress STAT-3 signaling pathway. Cells were divided into three groups:dimethyl sulphoxide (DMSO) group, cis-dichlorodiamine-platinum (DDP) group and WP1066+DDP group. Transcription level of miR-21 was assessed by real-time PCR, while the expression levels of STAT-3, p-STAT-3, tissue inhibitor of metalloproteinase-3 (TIMP-3) and matrix metalloproteinase-2/9 (MMP-2/9 ) were evaluated by Western blot assay. Matrigel matrix and transwell assay were used to determine cancer cell colony formation and invasive ability respectively. Expression level of miR-21 was examined by luciferase reporter gene as-say. Results Expression levels of STAT-3, pSTAT-3 and miR-21 were significantly suppressed by WP1066 treatment. The diameters of culture colony in cells treated with WP1066 and DDP were smaller than those in control group. The number of tongue cancer cells that migrated through the transwell membrane in WP1066 and DDP treated group was less than that in control group. Additionally, MMP-2/9 expression decreased while TIMP-3 increased dramatically in both cell lines in WP1066+DPP group compared to the other two groups. Conclusion Reduction of STAT-3 can sensitize oral squamous cell carcinoma to cis-dichlorodiamineplatinum via downregulating miR-21. Our study shows that DDP, in combination with WP1066, might be used as a potential target in the treatment of human oral squamous cell cancer.

Objective To explore the effect of renal sympathetic denervation (RDN) on cardiac remodeling in myocardial infarction (MI) rats and to investigate the related mechanism. Methods MI rats were induced by ligation of the anterior descending coronary artery. A total of 30 Wistar rats were randomly assigned to MI group (n = 10), MI + RDN group (rats with RDN one weekafter MI, n = 10), and Sham group (n = 10). Four weeks after modeling, the left ventricular cardiac remodeling and function of ratswere examined by echocardiography; the cardiac tissues in the infarct border zone were stained with Masson trichrome for fibrotic analysis. Protein expressions of collagen I, collagens H and transforming growth factor (TGF-(3i) were evaluated by immunohistochemistry. Results Compared with the MI group, the MI + RDN group had significantly increased ejection fraction (EF) and fractional shortening (FS) and significantly decreased left ventricular internal dimensions at end systole and end diastole (all P<0. 05). The results of Masson staining showed that RDN after MI attenuated the collagen deposition around the border area of the infarct region. RDN treatment also inhibited the protein expression of collagen I, H and TGF-β1 in the border area of MI rats (P<0. 05). Conclusion RDN treatment can attenuate cardiac remodeling and improve lett ventricular function after MI, which might be associated with the inhibition of myocardial TGFβ1 expression and the subsequent suppression of collagen deposition.

Objective To observe the clinical characteristics of kidney transplant patients combined with coronary heart disease, and to analyze the safety and efficacy of coronary angiography and percutaneous coronary intervention for them. Methods Six kidney transplant patients underwent coronary angiography in our department between 2005 and 2011. The clinical characteristics, coronary angiography data and prognoses of the patients were retrospectively analyzed. Results Three patients had normal coronary angiograms and the other 3 combined with diabetes mellitus also had coronary heart diseases as found by coronary angiography. One case had unstable angina and was implanted with a total of 5 stents during 2 sessions. Another case had acute inferior wall myocardial infarction (caused by complete occlusion of the right coronary artery) was implanted with 2 stents. The other case had acute non-ST-segment elevation myocardial infarction and coronary angiography revealed multi-segment diffuse lesions in 3 arteries; stent implantation failed in this patient. There was no significant difference in renal functions before and after coronary angiography in the 6 patients, and there was no deterioration of renal function during the follow-up. Conclusion Coronary angiography and percutaneous coronary intervention are safe and feasible for kidney transplant patients, and they can greatly improve the quality of life of the patients.

Objective To explore the significance of palpation-negative breast tumor resection with ultrasound-guided methylene blue labeling and percutaneous suture traction.Methods 46 cases of small breast tumors with the diameter from 0.5 cm to 1.0 cm were double labeled with methylene blue under the guide of ultrasound l hour preoperatively.The tumors were fixed,drew outward with percutaneous suture and resected accurately.All the 46 cases were rechecked by ultrasound to verify whether residual or false resection occurred 1 month later.Results All the 58 tumors of the 46 patients were accurately resected.No residue or false resection occurred.The average operation duration was 10 min to 20 min.Conclusion Palpable-negative breast tumor resection with ultrasound-guided methylene blue labeling and percutaneous suture traction has the advantages of small invasion,accurate positioning and short operation duration.