1.Application of Regulating Spleen on the Treatment of Repeated Respiratory Tract Infection in Children
Su ZHENG ;
Journal of Zhejiang Chinese Medical University 2006;0(03):-
This paper emphasizes constitution factor,the chief pathogenesis of repeated respiratory tract infection(RRI) of children;the main pathogenesis are Qi deficiency of spleen-lung and retention of phlegm.According to children's physiology feature,spleen is frequently insufficient and RRI Children are given too much antibiotic and bitter-cold herbs,that causes damage to stomach.Regulating spleen is expected to figure prominently in prevention and treatment of RRI children.Regulating spleen not only means reinforcing the spleen,but also promoting digestion to eliminate stagnation and regulating Qi and eliminating phlegm to restore the normal physiological function of spleen and stomach,strengthen children's constitution and immunity.There are 4 therapeutic principles of RRI children: activating spleen,promoting lung and resolving phlegm during the acute phase;reinforcing the spleen,eliminating stagnation and eliminating phlegm;reinforcing the spleen and promoting lung during the deferment period;reinforcing the spleen and enriching Qi for prevention.
2.Clinical study of jugular vein blood oxygen saturation in patients with acute cerebral infarction
Journal of Clinical Neurology 1995;0(04):-
0.05).Conclusion SjvO_2 and OEF may objectively reflect brain oxygen metabolism and cerebral blood flow after acute cerebral infarction. Furthermore, they are valuable indicators for predicting prognosis.
3.Determination of Gallic Acid in Milin Capsules by HPLC
Journal of Zhejiang Chinese Medical University 2006;0(02):-
[Objective] To develop an HPLC method for determining Gallic acid in Milin Capsules.[Method] The contents of Gallic acid were determined by HPLC.Agilent1200 HPLC.Chromatography column was ZORBAXSB-C18(4.6?250mm,5?m);mobile phase:methanol-0.2% phosphoric(6∶94);flow rate was 1.0ml/min;UV detection wave length was 270nm.[Results] The average recovery of Gallic acid was 99.10%,RSD was 1.07%.[Conclusion] This method was simple,quick,stable,accurate,can be used as a method for determining Gallic acid in Milin Capsules.
4.Clinical analysis of 16 cases of cryptococcus neofornans meningitis
Chinese Journal of Primary Medicine and Pharmacy 2006;0(03):-
Objective To investigate the clinical characteristics and diagnosis of some patients with cryptococcus neoformans meningitis(CNM).Methods The clinical features were analyzed and summarized for the findings of 16 CNM cases.Results The clinical cachexia manifestations were not found in the 16 CNM cases.The higher intracranial hypertension and the slight elevation of CSF protein content were found in the earlier period.The cryptococcus neoformans could be found in CSF through lots of lumbar puncture usually.Conclusion The symptom in the earlier period of cryptococcus neoformans meningitis is not typical.It is difficult to find the cryptococcus neoformans.In order to definite the diagnosis,more examinations of CSF must be done.
5.The Pre-assessment of Effectiveness on Constructive Project for Deep Treatment of Drinking Water
Yuming SU ; Buyun ZHENG ;
Journal of Environment and Health 1992;0(05):-
Objective In order to control the organic pollution in surface water sources in Jiaxing area, deep water treatment method was applied to treat drinking water. The effectiveness and correlative hygiene issues of the constructive project were pre,assessed. Methods On the basis of selecting and comparing some water factories in domestic and abroad which were similar with this constructive project, the assessment was synthetically carried out by surveying on the spot, checking blueprint, examining technological process and equipments layout, worked technique and materials, after experimental deep water,treatment was pup in to pilot scale test. Results The drinking water deep treatment method could eliminate organic substances effectively from original water. The results of the pilot test showed that the removed rates of CODMn, TOC, color degree and NH3,N through this measure were respectively 95.57%, 25.35%, 32.80% and 37.40% higher than those through route drinking water treatment measures. The measure could translate Ames test result into negative. The dosage of alum and chlorine could be reduced 33% and 50% respectively. Water purity and taste could be improved. The arrangement and layout of the facilities of this constructive project was advanced and reasonable, which was conformed to the hygienic requirements. Conclusion This constructive project could effectively solve the serious pollution problem of organic substances in water source in Jiaxing area and improve the purification effectiveness of drinking water and water quality. The occupational hazards that possibly present in the productive process of this project could be prevented. From the view of hygiene, this constructive project was feasible.
6.Application of sorting peripheral blood cells by flow cytometry in the detection of peripheral Mood cell sorting
Meijing ZHENG ; Jingjing DUAN ; Liping SU ; Yanfeng WANG ; Wen SU
Journal of Leukemia & Lymphoma 2010;19(3):159-160,165
Objective To apply fluo-rescencc-activated cell sorting(FACS) in sorting T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+), which establish the separating method of series of cells from human peripheral blood, so that the scientific research and clinical research could be carried out were specifically. Methods 10 cases of normal peripheral blood were collected and T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) were stained with florescence conjugated antibodies. The positive cells were sorted by FACS. Results Before sorting the peripheral blood, the proportion of the T lymphocyte(CD_3~+) and granulocyte (CD_(15)~+) in the leukocyte is 48.8 % and 30.8 %; after sorting by FACS, the purity of T lymphocytes (CD_3~+) is up to 98 % and the recovery is about 95 %; the purity of granulocyte (CD_(15)~+) is up to 97 % and the recovery is about 96 %. Conclusion The FACS could Mlow us to quickly sort T lymphocyte (CD_3~+) and granulocyte (CD_(15)~+) with higher recovery and higher purity from the peripheral blood.
7.Use of tumor proliferation marker ki-67 and PCNA in surgical pathology.
Chinese Journal of Pathology 2009;38(8):568-571
Astrocytoma
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metabolism
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pathology
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Biomarkers, Tumor
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metabolism
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Brain Neoplasms
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metabolism
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pathology
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Cell Proliferation
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Diagnosis, Differential
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Glioblastoma
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metabolism
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pathology
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Humans
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Ki-67 Antigen
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metabolism
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Melanoma
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metabolism
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pathology
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Meningeal Neoplasms
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metabolism
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pathology
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Meningioma
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metabolism
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pathology
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Nevus
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metabolism
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pathology
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Proliferating Cell Nuclear Antigen
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metabolism
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Skin Neoplasms
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metabolism
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pathology
8.Diagnostic value of the vascular endothelial growth factor in ascites
Li ZHENG ; Weichang CHEN ; Su YAN
Chinese Journal of Practical Internal Medicine 2002;0(08):-
Objective To study to the differential diagnostic value of the VEGF in ascites and to investigate the clinical significances of VEGF combined with CEA measurement.Methods The VEGF and CEA levels in ascites were measured by ELISA in 58 ascites specimens.Results The VEGF level in malignant ascites was significantly higher than that in nonmalignant ascites (P
9.The Trend of the Satisfaction of Physical Self of Middle School Students in Guangzhou
Sian XU ; Xue ZHENG ; Binyuan SU
Chinese Mental Health Journal 2002;0(12):-
Objective:To study the satisfaction of the physical self of the junior students in Guangzhou. Methords:582 middle school students were measured by the physical self scale. Results: The junior students in Guangzhou felt a medium satisfaction at their physical self, and the highest satisfaction was appearance(3.9?0.8),then the lowest was sexual characters(3.0?0.7). The male junior students felt obvious higher satisfaction with their physical self than the females, including appearance(3.9?0.7/3.8?0.7,F=7.39,P
10.A sensitive and specific method for detection of nuclear transcription factor C/EBP? based on the complexes of DNA-binding-C/EBP?
Jianrong SU ; Zheng ZHANG ; Dongxu SUN
Chinese Journal of Laboratory Medicine 2003;0(12):-
Objective To establish a perfect method that is based on the complexes of DNA-binding-C/EBP? for detection of nuclear transcription factor C/EBP? (CCAAT enhancer binding protein ?). Methods To search specific oligonucleotides sequence and establish a labeling method of consensus oligonucleotides and the standard curve was regressed.The electrophoresis conditions were optimized.Results The combination of 20pmol of ?- 32 P-ATP and 4 pmol of C/EBP? oligonuleotides in reaction can express the highest probe activity and corporation effect.The linearity was established (r2=0.975). 0.5?g of nuclear extract can be detected and bands are clear and specific.Conclusion The method is good at specificity. It can be a method to detecting nuclear factor C/EBP? or its DNA-binding site.