The aim of joint development and sharing of institutional repository is to reveal and use the primary literature in military colleges and universities, and help scientific researchers, teachers and students to discover and use such re-sources .The ideas on joint development and sharing of institutional repository in military colleges and universities were thus analyzed, and the related problems were elaborated with suggestions put forward for their solution.

BACKGROUND:The effects of posterior decompression fixation in the repair of thoracolumbar fracture are affirmative. The fixation was divided into short-segment and long-segment pedicle screw fixation according to different treatment methods. The therapeutic effects of above two methods are controversial in the clinic at present. OBJECTIVE:To compare and observe the anterior vertebral compression ratio and the changes in Cobb angle after posterior short-segment and long-segment pedicle screw placement fixation in the repair of thoracolumbar fracture. METHODS:120 patients with thoracolumbar fracture were enrol ed in this study, and assigned to two groups according to fixation methods. Study group received posterior short-segment pedicle screw fixation. Control group received posterior long-segment pedicle screw fixation. Anterior vertebral compression rate and Cobb angle were observed before fixation, immediately and 12 months after fixation in both groups. RESULTS AND CONCLUSION:There was no significant difference in anterior vertebral compression rate and Cobb angle between the two groups before fixation (P>0.05). Anterior vertebral compression rate and Cobb angle were significantly smal er in the study group than in the control group immediately and 12 months after fixation (P<0.05). Besides, surgical time and blood loss volume were less in the study group than in the control group (P<0.05). These results indicated that posterior short-segment pedicle screw fixation for treatment of thoracolumbar fracture can effectively improve kyphosis, recover vertebral compression degree, and its surgical time is short and blood loss volume during surgery is little, so it is better than posterior long-segment pedicle screw fixation.

Objective To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis (PFGE REA) and random amplified polymorphic DNA (RAPD) analysis for molecular typing of Neisseria gonorrhoeae isolates. Methods Genomic DNA from 36 Neisseria gonorrhoeae isolates were digested with SpeI and analysed by contour clamped homogeneous electric fields electrophoresis. The 36 isolates were also characterised by RAPD analysis with arbitrary primer OPA 03. Results 12～19 DNA fragments from 5 to 600kb were obtained among the 36 isolates after PFGE REA. The 36 isolates were discriminated into 20 PFGE patterns. Amplification of Neisseria gonorrhoeae DNA with primer OPA 03 produced 15 different DNA fragments (280～1900bp), and 3～9 fragments per strain could be seen. The 36 isolates showed 18 different RAPD patterns. Strains sharing common auxotypes and antibiotic spectrum could be differentiated by PFGE REA and RAPD analysis. General agreement was found between these two techniques. Isolates from different geographic areas and even some isolates in the same area showed considerable amount of DNA polymorphism. In addition, some isolates shared common or very similar patterns were unique to a given geographic area. Conclusion It is concluded that both PFGE REA and RAPD analysis are useful, sensitive molecular techniques for differentiation of clinical isolates of Neisseria gonorrhoeae. They should be helpful in the investigation of strain origin, clonal relation among strains and spread of antibiotic resistance. Compared with PFGE REA, RAPD analysis is faster, relatively simple and more economical.

Objective: To investigate the effect of soy isoflavones extract on bone loss induced by estrogen deficiency. Methods: 87 postmenopausal women were randomly assigned in single blind manner, to three treatment groups with daily intake of placebo, 84 mg and 126 mg soy isoflavones respectively. Additionally a positive control group was 10 women was given daily 2.5 mg estrogen. Bone mineral density (BMD) of the lumbar spine, neck of femur and Ward`s triangle were measured at baseline and post treatment after 24 wk . Results: BMD in all sites in both isoflavones treated and estrogen treated groups did not change significantly. However, the BMD of the lumbar spine decreased in the placebo group (P

AIM: To establish a method of determining eucalyptol, camphor, menthol and borneol in Ganmaokang Spray. METHODS: GC was conducted on a EC-WAX column(30 m?0.53 mm?1.2 ?m) and naphthalene was adopted as internal standard. RESULTS: Eucalyptol, camphor, menthol and borneol respectively showed a good linear relationship in the following ranges of concentration:0.384 48-0.987 12 mg/mL,0.240 6～(0.561 4 mg/mL,)0.367 56-0.857 6 mg/mL and 0.135 6-0.316 4 mg/mL. The average recoveries were obtained as 98.4%, 98.2%, 98.5% and 98.2%(n=5). CONCLUSION: The method is simple, sensitive, accurate and suitable for the determination of four constituents in Ganmaokang Spray.

Polysaccharides is one of the main bioactive components of Cordyceps species, because of the potential clinical value with stronger anti-tumor, such as anti-neuroblastoma, anti-melanoma, anti-lung cancer, anti-colon cancer and so on, its have received widespread attention in biomedical field and increasing research in last decades. According to structural elucidation, this review gives a systematic literature overview on antitumor mechanism of Cordyceps species-derived polysaccharides from three aspects, including inhibition of tumor cell growth, enhancement of immunomodulatory activity and reduction of tumor metastasis. Finally, it also puts forward some scientific problems for follow up research.

Objective　To analyse the state of the administration of clinical med icine in some children's hospital. Methods　Prevalence study was use d. Results　The administration of antibiotic and vitamin were domina nt among inpatients, and their utilization rates were 84.26%, 79.08% respec tively. 1 643 among 1950 of cases used antibiotic agents, the utilization rates of penicil ins, cephalosporins and antiviral medicine were 53.38%, 31.21% and 30.74% respec tiv ely. The administration of combined antibiotic added up to 71.88%. Conclusions　The phenomenon of irrational administration of medicine wa s existing in the children's hospital. So it should be perfected on the rules an d regulations for the administration of medicine, and the management of medicine should be strengthened.

After the concept of data curation and its application in foreign countries were described , the key points of data curation for data service in special libraries and related problems were analyzed according to the overall needs and changes of users in special libraries.

Objective To investigate the prevalence of sick building syndrome (SBS) and explore the related risk factors in the people working in office environment in Beijing. Methods A cross-sectional study design was made in the present paper in April 2009. One hundred and two employees in 16 small and moderate offices in three different buildings in Beijing were investigated with the questionnaire for their subjective symptoms, environmental perceptions, psychosocial information and health situation. The indoor environmental factors of the buildings were monitored based on the standardized procedures at the same time. Generalized linear model and multilevel model were used for the data analysis. Results The prevalence rate of symptoms for sick building syndrome was 67.4%. The prevalence rate of SBS symptoms was positively associated with the self-feelings of the subject to high temperature, insufficient ventilation indoors, work stress, satisfaction for job and health complains, as well as with using photocopier in office by ANOVA(P

Objective To study human prostate cancer tissue specific hytk gene therapy vector using prostate specific antigen (PSA) promoter,and to determine its feasibility. Methods A prostate tissue specific hygromycin phosphotransferase thymidine kinase fusion gene(hytk)vector was established with the substitution of CMV promotor in tgCMV/hytk vector with PSA promotor.The vector was transferred into prostate carcinoma cell lines with FuGENE TM 6.Antitumor effects were observed after GCV treatment. Results PSA promoter that actively drives hytk gene was expressed only in the PSA producing prostate cell line(LNCaP).In vitro experiments demonstrated the LNCaP cells transferred by HyTK gene were killed in the presence of GCV treatment. Conclusions Hytk based vector harboring PSA promoter is a novel ideal candidate vector for prostate carcinoma gene therapy.