Objective T o investigate the serum IgE w ith various postm ortem intervals (PMI) in guinea pigs due to sudden death from anaphylactic shock and to explore the effect of refrigeration of corpse on serum IgE level and its application value in forensic m edicine. Methods T he anim al death m odels of anaphylactic shock w ere established. T he corpses w ere preserved at room tem perature (20℃) for 6 h and then refrigerated at 4℃. T he serum w as sam pled at 6, 12, 24, 36 and 48 hours after death. T he IgE level of serum w as detected w ith ELISA . The control group w as also established. Results The serum IgE level had significant difference betw een the experim ental group and the control group (P<0.05). T here w as no significant difference am ong the experim ental groups at 6, 12, 24, 36 and 48 hours post-m ortem (P>0.05). Conclusion If the corpses w ere placed in 4℃ conditions 6 hours after anaphylactic death, the serum IgE still show s a good m arker w ithin 48 h for forensic investigation.

Objective:To learn and master the application of microwave technology in medical electron linear accelerator tube. Methods: Introduced the structure and principle of medical electron linear accelerator, traveling wave accelerating tube and standing wave accelerating tube;compared the structure, stable system and transport system of traveling wave accelerating tube standing wave accelerating tube. Results:standing wave accelerating cube have higher efficiency, shorter accelerating cube and electron gun, but have higher demand for pulse modulator, automatic frequency stabilization, the deflection system and microwave transmission systems. Traveling wave accelerating cube have lower efficiency, but have better energy spectrum and easy to adjust. Conclusion:Both traveling wave accelerating cube and standing wave accelerating cube were used in medical electron linear accelerator nowadays, it's easy to master medical electron accelerator after knowing the application of microwave in medical electron linear accelerator.

Objective To analyze the clinical character of uveitis in second hospital ot Jilin university.Methods Retrospectively analyze the clinical data of uveitis patients referred to from Second Hospital of Jilin University from September 2009 to September 2014.According to anatomical location,the manifestation of these uveitis patients were divided into anterior uveitis,panuveitis,intermediate uveitis and posterior uveitis.To discuss the possible causes of these patients according to the general information and relevant clinical laboratory examinations results.Results There were 1215 cases in this study,which included 587 male,accounting for 48.31％;and 628 female,accounting for 51.69％.The ratio of male-tofemale was 0.93∶1.The range of the age of these patients was from 4 to 91 years old.The mean age of these patients at the onset of these disease was (41.43±14.20) years.Of the 1215 cases,40 male and 43 female were younger than 20 years.The ratio of male-to-female was 0.93∶ 1;412 male and 396 female were between 21 and 50 years old.The ratio of male-to-female was 1.04∶ 1;135 male 189 female were older than 50 years.The ratio of male-to-female was 0.71∶1.There were 572 cases of anterior uveitis,accounting for 47.08％;527 cases of panuveitis,accounting for 43.37％;52 cases of intermediate uveitis,accounting for 4.28％;64 cases of posterior uveitis,accounting for 5.27％.703 cases had etiological diagnosis according to the clinical character and the auxiliary results,accounting for 57.68％.Vogt-koyanagi Haradal (VKH) syndrome,ankylosing spondylitis associated with uveitis and Behcet' s disease were the common entity,accounting for 30.44％,19.77％ and 14.22％ respectively.Conclusions The mean age of these patients in this study was older,compared to other reports.Female patients were more than male,especially in these patients older than 50 years.VKH syndrome,ankylosing spondylitis associated with uveitis and Behcet's disease were the common entities.

Objective To observe the effects of exogenous basic fibroblast growth factor (bFGF) on radiation-induced apoptosis of C17.2 neural stem cells(NSCs) and explore the relationship between bFGF and Hes1,RBP-JK protein.Methods The cell viability was assessed using the MTT assay.After the cells attach to the flasks they were irradiated by the linear accelerator.And 5 min later,different concentrations of bFGF in accordance with the experimental design and cultured cells 48 h.Extracted total protein of each group and Western blot analysis showed the changes of the Hes1,RBP-JK protein.Results Compared with the control group,irradiation group cell growth was inhibited,the OD of 0 ng/ml bFGF group was 0.61±0.81,the OD of 80 ng/ml bFGF group was 1.21±1.01 and the control group was 1.51± 1.13.Compared with the control group,all groups showed statistically significant difference (P＜ 0.05).Western blot analysis showed that with the increasing concentrations of bFGF,RBP-JK expression gradually decreased,but Hes1 expression gradually increased.Conclusion Exogenous basic fibroblast growth factor (bFGF) can inhibit apoptosis of C17.2 NSCs.bFGF can regulate Notch signaling pathway downstream proteins Hes1,RBP-JK expression and inhibit neural stem cell apoptosis which were irradiated.

OBJECTIVE: To observe the clinical efficacy of Sodium Hyaluronate Eye Drops in combination with Recombinant Bovine Basic Fibroblast Growth Factor Eye Drops for dry eye after undergoing eximer laser in-situ keratomileusis(LASIK).METHODS: 62 cases(100 eyes) of patients with dry eye after undergoing LASIK were randomly divided into two groups: 32 cases(53 eyes) in the treatment group were treated with Sodium Hyaluronate Eye Drops in combination with Recombinant Bovine Basic Fibroblast Growth Factor Eye Drops,and 30 cases(47 eyes) in the control group with Sodium Hyaluronate Eye Drops alone(4 times/day,1～2 drops/time) for 2 months.The drug efficacy was observed for 1～3 months since 1 week after the initiation of medication.RESULTS: The total effective rate in the treatment group vs.the control group was 98.11% vs.89.36%(P

OBJECTIVE: To investigate the serum IgE with various postmortem intervals (PMI) in guinea pigs due to sudden death from anaphylactic shock and to explore the effect of refrigeration of corpse on serum IgE level and its application value in forensic medicine. METHODS: The animal death models of anaphylactic shock were established. The corpses were preserved at room temperature (20 °C ) for 6 h and then refrigerated at 4 °C. The serum was sampled at 6, 12, 24, 36 and 48 hours after death. The IgE level of serum was detected with ELISA. The control group was also established. RESULTS: The serum IgE level had significant. difference between the experimental group and the control group (P < 0.05). There was no significant difference among the experimental groups at 6, 12, 24, 36 and 48 hours post- mortem (P > 0.05). CONCLUSION If the corpses were placed in 4 °C conditions 6 hours after anaphylactic death, the serum IgE still shows a good marker within 48 h for forensic investigation.
Anaphylaxis/blood* ; Animals ; Autopsy/veterinary* ; Death, Sudden ; Enzyme-Linked Immunosorbent Assay ; Forensic Medicine ; Guinea Pigs ; Immunoglobulin E/blood* ; Postmortem Changes ; Refrigeration ; Serum

0.05).Conclusion The parameters of CT perfusion can be regarded as a surrogate index of tumor angiogenic activity pre-and post-radiotherapy in nasopharyngeal carcinoma.

Glioblastoma(GBM)is one of the most common primary intracranial tumor that has high de-gree of malignancy ,invasive ability and a fatal prognosis .In recent years ,with the development of modern technol-ogy in biomedical sciences ,the understanding on GBM has developed gradually from pathological diagnosis to mo -lecular classifications ,which is based on the molecular characteristics of genetic signatures .Based on gene expres-sion and DNA methylation patterns , primary glioblastoma is divided into four subtypes , including the classical , neural,proneural and mesenchymal .These molecular classifications are closely relevant to the biological charac-teristics of glioblastoma .This review briefly introduces the molecular classifications of primary glioblastoma , but mainly focuses on the changes of the major genetic EGFR ,PTEN and PI3K,CDKN2A in the classical subtype of GBM,and discusses the treatment strategies for primary glioblastoma .

Objective To investigate early changes of angiopoietin-2(Ang-2)in multiple trauma patients and assess its clinical significance.Methods Forty-five multiple trauma patients aged 2060 years admitted to the hospital within one hour after injury were randomly divided into three groups according to injury severity score(ISS).Blood specimens were obtained immediately upon arrival in the emergency department and plasma samples were assayed for comparing changes of Ang-2,TNF-a and IL-6.Meanwhile,plasma level of Ang-2 was measured and analyzed under different oxygenation index,shock index and base deficit.Results Plasma level of Ang-2 was positively correlated with ISS(P ＜ 0.01)and was concordant with the plasma levels of TNF-a and IL-6(P＜0.01).Furthermore,plasma level of Ang-2 was elevated upon increase of shock index or decrease of oxygenation index(P ＜ 0.01).Plasma level of Ang-2 was elevated with the increase of base deficit(P ＜ 0.01).Conclusions High level of Ang-2 is a marker of endothelial activation and dysfunction early after trauma.Ang-2 is related tightly with the injury severity,inflammation factors,systemic oxygenation and tissue hypoperfusion and may have a tight relation with pathophysiological development and clinical outcome after trauma.

Objective To explore the effectiveness of sandplay therapy combined with family counseling in children with school phobia and its influence of child′ self-consciousness. Methods Integrative sandplay therary with family consulting were used to treat 28 patients with school phobia regularly for 2 months. Sandplay and family consulting therapy were given once a week for 45 minutes . Clinical outcomes were assessed using CGI-GI and Piers-Harris children′s self-consciousness scale before and after treatment as well as 3 months posttreatment. Results Overall response rate was 85%. In addition, the physical appearance and characteristic factor before and after treatment were no significant difference (P>0.05). The rest of the various factors and total score compared with pre-treatment significantly improved (P<0.05). After treatment for 3 months, every factor in self-consciousness of children and total score were no significant difference (P>0.05). Conclusion Integrative sandplay therapy with family counseling has better and long-lasting treatment effect to self-consciousness of children with school refusal.