Two-dimensional electrophoresis performed on a microfluidic chip could reduce the consumption of sample and reagents, realizing high-throughput analysis in a very short time. During the isoelectric focusing ( IEF) separation, the buffer for the two different separation methods should be kept from being mixed. After the IEF, the protein was transferred from the IEF channel into the second dimension channels. Based on the pseudo valve structure, this paper reported a method to deposit a titanium dioxide membrane at the interface of two dimensional channels, which enhanced the performance of the pseudo valve. The IEF electrophoresis and SDS-polyacrylamide gel electrophoresis ( SDS-PAGE ) procedures were characterized respectively. The total separation took 10-15 min, and the protein's migration rate was linear with the migration time, and inversely proportional to the logarithm of protein molecular weight. Based on the above results, the differential two-dimensional electrophoresis was performed to test the deviation between different measurements.

Objective To explore framework and measure of vocational psychological quality for postgraduate students majoring in med-ical sciences in China. Methods The framework of vocational psychological quality had been developed based on the grounded theory. A structured and standardized questionnaire had been developed via field test for 95 postgraduates and interviews with 24 experts. A total of 1142 postgraduates had been measured with the final questionnaire. Results The standardized questionnaire had been developed including 3 subscales from factor analysis, such as vocational cognition, vocational personality and vocational adaptation, with 13 factors and 62 items in total. The Cronbach'sαcoefficient was 0.902 for the whole questionnaire, and the split-half reliability was 0.747. The correlation coeffi-cients between subscales and whole questionnaire was 0.401 to 0.929. Conclusion Vocational cognition, vocational personality and vocation-al adaptation are the main constituents of the vocational psychological quality for postgraduates majoring in medical sciences. The question-naire is acceptable in content and structure validity and internal consistency reliability.

Some problems have influence on the quality of Clinical practice teaching of foreign students,including language communication barriers,teacher shortage and so on.To solve these problems,we worked hard on the rationale use of teachers,improvement of the teacher’s English level,giving lecture and collecting opinions to improve the quality of teaching.

Objective To investigate the HAART in PMTCT different intervention time of blocking effect. Methods 49 cases who received out -patient examination and hospital delivery of HIV positive pregnant women were selected,the intervention time pregnant 14 weeks,pregnant 28 weeks.The patients were divided into three groups,group A (n =20),group B (n =19),group C (n =10).The safe delivery,the newborn 50 out of 18 months of tracking and detection,and the results were analyzed.Results Three groups of antiviral HIV infected maternal immune status (P >0.05 ),antiviral CD +4 cell number variation had no statistical significance (P >0.05 ),the blocking effect was A group of infants 0 infection.In group B,1 cases of infant infection.Group C 2 cases of infant infection.Compared between group A and group B,the difference was not statistically significant (χ2 =0.97,P >0.05),compared between group B and group C,the difference was not statistically significant (χ2 =1.40,P >0.05). Compared between group A and group C,the difference had statistical significance (χ2 =4.08,P <0.05 ). Conclusion HAART for blocking HIV mother to child transmission effect is very significant,if in 14 weeks pregnant initiation of HAART,the infant HIV infection rate is lower,recommended the early antiviral treatment and after childbirth to strengthen follow -up and management.

Objective To study the influence of hydrocortisone sodium succinct by intravenous infusion or continuous intravenous micro pumping on the vital signs,shock duration,blood glucose and mortality in the patients with severe sepsis secondary shock.Methods Ninety patients with septic shock treated in our hospital from June 2014 to September 2016 were selected and divided into the control group and observasion group by using the random number table method,45 cases in each group.The control group was given hydrocortisone sodium succinct 200mg/d by slow intravenous drip for 2h,while the observasion group was given hydrocortisone sodium succinct 8.33mg/h by continuous intravenous micro pumping.The two groups were treated for continuous 5 d.The blood glucose,hemodynamics and prognosis were compared between the two groups.Results In the observasion group,the blood glucose indicators such as the time window of high blood glucose,LAGE,MBG and CV levels were lower than those in the control group,the difference had statistical significance (P<0.05).The hemodynamic indicators such as HR,CVP and MAP between the two groups before treatment and at 6,24 h and 5 d after treatment had no statistical difference between the two groups (P>0.05).The shock duration,ICU stay length,total hospital stay and 28 d mortality in the observasion group were lower than those in the control group,but the difference between the two groups had no statistical significance (P>0.05).Conclusion Continuous intravenous micro pumping of hydrocortisone sodium succinct can stabilize blood glucose fluctuation range in the patients with septic shock,but the hemodynamic indexes and prognosis have no obvious improvement.

OBJECTIVE: To review the development of Dendrobium officinale Kimura et Milated health foods. METHODS: Through the database of Slate Food and Drug Administration and State Intellectual Property Office website, all health foods mainly composed of D. officinale and related patents were listed and analyzed. RESULTS AND CONCLUSION: D. officinale related health foods can be further developed by continuous improvement their health of functions, formulation composition and dosage form. Combined with novel foods development, the function and form of Dendrobium officinale related health foods will be diversified and even enter the international market.

Objective: To observe the inhibitory effect of Fuzheng Xiaoliu Decoction (FXD) on cerebral glioma. Methods: Rat glioma orthotopic transplantation model was established by inoculating C6 glioma cells to brain right caudate nucleus in situ of Wistar rats, and the rats in treatment group were ig administered with FXD for consecutive 14 d. After the last administration, blood was taken to measure whole blood viscosity and plasma viscosity, serum immunoglobulin (IgG, IgA, and IgM), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), and interferon-γ (IFN-γ) levels; All rats were sacrificed after last administration to collect whole blood; The brain was taken and weighed, brain index was calculated, tumor length and short diameters were measured, and tumor volume and inhibitory rate were calculated; The tumor tissue was taken, vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2), insulin-like growth factor-1 (IGF-1) levels were determined. Results: All three doses (1.2, 2.4, and 3.6 g/kg) of FXD could inhibit rat glioma tumor volume. It could reduce the contents of IGF-1, PGE2, VEGF in tumor tissue and serum IgG, elevated the level of serum IgA, IgM, TNF-α, IL-2, and IFN-γ to varying degrees. FXD (3.6 g/kg) can reduce whole blood viscosity at low shear (P < 0.05, 0.01). Conclusion: FXD has significant antitumor effect on C6 glioma, and the mechanism of antitumor effect may be the inhibition of tumor angiogenesis and tumor cell proliferation, enhancing immune response, and reducing blood viscosity.

Objective: To observe the effects of Jimaitong Tablet on the blood pressure, liver and kidney function, and vasoactive substances of the rats with long-term drinking. Methods: Forty SD rats were divided into four groups including control, model, Jimaitong Tablet, and Amlodipine groups (n = 10). The rats drinking spirits freely, and the alcohol concentration increased in gradient. After 4 weeks of modeling, the rats were ig administered successively for 49 weeks, once daily. The blood pressure (such as SBP, DBP, and MBP) and blood biochemical markers of liver and kidney function (such as ALT, AST, Cr, and UA) had been regularly monitored. After the last administration, the rat blood was collected from the inferior vena cava plexus, and the angiotensin II (AngII), aldosterone (ALD), and thromboxane B2 (TXB2) levels in plasma were measured. Results: The blood pressure (SBP, DBP, and MBP) of rats increased after 12-week continuous drinking and was stable until week 53. After the administration of Jimaitong Tablet for 15 weeks onwards, the blood pressure (SBP, DBP and MBP) of rats with long-term drinking could be significantly reduced. It showed stable antihypertensive effect during the administration for up to 49 weeks (P < 0.01, 0.05). Jimaitong Tablet could improve ALT and AST levels in serum to varying degrees (P < 0.01, 0.05) at the administration of 38-48 weeks. Jimaitong Tablet could reduce ALD, AngII, and TXB2 levels (P < 0.01, 0.05) in plasma of the model rats during the administration of 49 weeks. Conclusion: Jimaitong Tablet has good effect on hypertensive rats induced by long-term drinking, and has a distinct improving effect on liver injury in rats. Its mechanism of the antihypertensive effect may be related to the renin-angiotensin-aldosterone system and thromboxane level.

Objective To investigate the mechanism underlying the protective effect of hyperbaric oxygen (HBO) in secondary spinal cord injury (SCI). Methods Models of acute SCI were established in 96 SpragueDawley rats using Allen's dropping weight technique. The rats were then divided into a HBO group, a high pressure nitrogen normal oxygen group, a normal pressure oxygen group and a normal pressure air group. The injured spinal cords were sampled for morphological studies at the 1 st, 3rd, 7th and 14th day after injury. Apoptotic cells were labeled using the TUNEL technique, and the expression of caspase-3 was detected. The neurological functionality of the spinal cord was assessed by open field locomotor evaluation ( the BBB score). Results The expression of caspase3 in the HBO group decreased significantly more than in the other groups after injury. The number of TUNEL-positive cells was significantly lower in the HBO group as well. Neurological function improved significantly after HBO therapy. Conclusions HBO can down-regulate the expression of caspase-3 and inhibit cell apoptosis in rats after SCI.The protective effect of HBO was related with the oxygen level.

Objective To study the tumorigenetic characteristics of side population (SP) cells and their exsistence in gastric cancer cell lines and tissues.Methods Flow cytometry and the DNA-binding dye Hoechst 33342 staining were used to analyze sorted SP cells in 3 gastric cancer cell lines(SGC-7901,BCIC-823 and MKN28).SP cells and non-SP cells(main population cells)isolated from SGC-7901 cell line weresubcutaneously injected into 36 nude mice with 500,5000 or 50 000 cells per mouse,respectively.Thetumorigenity was observed 8 weeks after injection.The expression of ATP-binding cassette sub-family Gmember 2(ABcG2)mRNA in three gastric cell lines and its level in gastric cancer tissues were detected by real-time PCR and immunostaining.respectively.Results The proportion of SP cells was accounted for 1.0%in SGC-7901 cells and 1.3%in BGc-823 cells.No SP cell was found in MKN28 cells.As low as 500 SP cells or 50 000 non-SP cells could initiate tumors in mice.The expression of ABcG2 mRNA was higher in SGC-7901(O.162)and BGC-823(O.096)cell lines than that in MKN28 cell line(0.005).The value of AtCG2 mRNA/beta-aetin mRNA and protein in gastric cancer tissues was different from those in gastritis tissues.Condasimm SP cells have strong ability of tumorigenesis compared with non-SP cells.The expression of ABCG2 is found in gastric cancer tissues and part gastritis tissues.The more the SP cells in gastric cell lines,the higher the expression of ABCG2.