Objective: To study the prevention and rehabilitation of Shengxue Pills on liver, spleen, bone marrow cells, peripheral blood lymphocyte of mice injured by low-dose radiation and their mechanisms. Methods: Mice were randomly divided into five groups: control, Shengxue Pills prevention, Shengxue Pills rehabilitation, model 1 (corresponding to rehabilitation), and model 2 (corresponding to prevention) groups. Using the ELISA method to detect the activity of hydroxyl radical, superoxide anion radical, and ornithine decarboxylase (ODC) in liver and spleen cells. The bone marrow cells and peripheral lymphocyte micronucleus rate were observed under high power lens. Results: Compared with control group, the amounts of hydroxyl radical and superoxide anion radical in the two model groups were obviously increased. Compared with the corresponding model groups, the above indexes in prevention group and rehabilitation group were significantly decreased. Compared with the control group, the activity of ODC in mice of the two model groups was obviously enhanced, but bone marrow cells and peripheral lymphocyte micronucleus rate were increased. Compared with the corresponding model groups, the activity of ODC in mice of prevention and rehabilitation groups was weakened, and bone marrow cells and peripheral lymphocyte micronucleus rate were decreased. But each index of spleen cells showed no significant difference. Conclusion: Shengxue Pills could effectively prevent and rehabilitate the damage in liver cells, peripheral blood lymphocytes, and bone marrow cells of mice injured by low-dose radiation.

AIM: To research the application analysis of trabeculectomy combined with phacoemulsification treating patients with primary angle-closed glaucoma ( PACG) and cataract.
METHODS: Seventy-three patients from September, 2011 to September, 2013, with primary angle- closed glaucoma and cataract, were selected in our research. Patients were all treated with trabeculectomy combined with ultrasonic emulsification, and the therapeutic effect was observed.
RESULTS: The effects of trabeculectomy combined with phacoemulsification treating primary angle-closed glaucoma and cataract in reducing intraocular pressure were better, and postoperative visual acuity results of the research objects were satisfactory. And it could deepen the central anterior chamber depth effectively. All the research objects had few complications.
CONCLUSION: Trabeculectomy combined with phacoemulsification treating primary angle - closed glaucoma and cataract can achieve a good result for reducing intraocular pressure, postoperative visual acuity recovery and correction, and the surgical success rate is high, with low complication rates. It is worthy of widely clinical application.

Adult ; Brucellosis ; Humans ; Male ; Middle Aged ; Occupational Diseases

Objective To investigate the disease control status of patients with diabetes and chronic kidney disease (DMCKD) so as to provide evidence for patients' management.Methods All medical records during May 2007 to May 2009 were reviewed and 345 outpatients were retrospectively investigated from Department of Nephrology.Body mass index (BMI),blood pressure (BP),HbA1c,fasting plasma glucose (FPG),serum lipids,renal function were evaluated,etc.Results Among them,65.8％ patients were overweight or obesity.The standard control rate of FPG,HbAlc,BP,triglyceride (TG),24 hour urine protein loss was 28.7％,43.2％,21.7％,33.7％ and 54.3％,respectively.Their BMI level was positively correlated with FPG,BP and TG( r =0.287,0.137,0.328,respectively,P ＜ 0.05 ),while FPG was positively correlated with HbA1c and TG(r =0.434,0.192,respectively,P ＜0.01 ).DM duration was positively correlated with FPGand HbA1c (r =0.171,0.117 respectively,P ＜ 0.05 ).Conclusions The disease control status of DMCKD patients was poor and disease indicators are influencing with each other.So healthcare provider should enhance effective health education and management to promote patients' self - management and to retard diseases progression.

Objective To evaluate the value of ultrasound (US) in assessment knee joint inflammation in patients with juvenile rheumatoid arthritis(JRA).Methods US scans of the knees obtained in 30 children at clinically active stage; JRA was compared with those obtained in 30 healthy children and 10 JRA patients in clinical remission.Results Changes in synovial membrane thickness and presence of fluid in suprapatellar bursa showed statistically significant differences between JRA patients with active disease and the other subjects.Alterations in contour of the articular cartilage were demonstrated in 10 knees of patients with JRA.Conclusion US is a simple sensitive and reliable methods for the assessment and monitoring of knee joint involvement in JRA.

Novel aminopropyl-functionalized periodic mesoporous organosilicas (APMO) were successfully prepared by co-condensation of 1,2-bis (triethoxysilyl) ethane (BTSE) and 3-aminopropyltriethoxysilane (APTES) with the template of cationic surfactants cetyltrimethylammonium chlorine (C_(18)TAC) in basic medium.With the characterization measurements of powder X-ray diffraction (XRD),FT-IR and scanning electron microscopy (SEM) so on,APMO had such advantages as good monodisperse microshperes,uniformly orderd mesopore and large specific surface areas.These mesoporous materials were directly considered to be the chromatographic stationary phases and analyzed by HPLC evaluation.During the research,the as-prepared APMO column had better permeability and three polycyclic aromatic compounds realized separation.In addition,the as-prepared APMO column had certain advantages in fast analysis.

Pseudorabies virus (PRV) is a swine herpesvirus of the Alphaherpesvirinae subfamily and a pathogen of swine resulting in devastating disease and economic losses worldwide. Cre/loxP site-specific system has the character of site specific, time specific, tissue specific and high efficiency in recombination, which makes this system universal in vivo and in vitro recombination of bacteria, fungus, plants, insects and mammals. A recombinant PRV which contain a loxP site in TK locus by using Cre/LoxP recombinant system was construsted. A pair of primers were synthesized according to the pEGFP-C1 sequence published on GenBank, and were used to amplify the EGFP gene expression cassette with two loxP sites flanking each side. This target gene was cloned into pSKLR, the resulting transfer vector pSKLR-GFP-loxP was then cotransfected into 293T cells with PRV SH strain genomic DNA. The recombinant virus rPRV1 was selected and purified in TK-143 cells by choosing fluorescent expressing plaques. Cre expression vector pOG231 was cotransfected into 293T cells with rPRV1 genomic DNA. The second recombinant virus rPRV2 was obtained, which contains only one loxP site in TK locus. Sequencing results of rPRV2 TK gene indicated that 34bp loxP site was inserted into rPRV2 genome and there were 270bp deletion in TK gene. PCR amplifying different generations of rPRV2 TK gene showed that the mutant was stable when passages in RK-13 cells. TCID_ 50 assay indicated that rPRV2 grows well on RK-13 cells. The LD_ 50 test results on BALB/C mice suggested that the virulence of rPRV2 was reduced. As a conclusion, the report gene GFP expression cassette was removed successfully from rPRV1 genome and only one LoxP site was leaved in rPRV2 genome by using Cre/LoxP recombinant system.

Objective: To investigate the clinical efficacy of Wei Chang An Pills combined with Artemether Injection in the treatment of malaria (gastrointestinal type). Methods: Patients (253 cases) with malaria (gastrointestinal type) in Hospital de L'amitie Sino-Congolaise in The Republic of Congo from July 2013 to December 2014 were randomly divided into control and treatment groups. Patients (130 cases) in the treatment group were im administered with Artemether Injection and po administered with Wei Chang An Pills. Patients (123 cases) in the control group were im administered with Artemether Injection and po administered with Vitamin B6 Tablets. Patients in both two groups were treated for 7 d. After the treatment, the efficacy was evaluated, and the improvement of nausea, vomiting, abdominal pain, and diarrhea was observed, white blood cells and red blood cells in feces were simultaneously detected, numbers of plasmodium in peripheral blood were counted. Results: After the treatment for 5 d, the clinical symptoms of all the patients (130 cases) in the treatment group disappeared. After the treatment for 7 d, clinical symptoms of patients (87 cases) in the control group disappeared, and there were differences between two groups (P < 0.05). Compared to the control group, white blood cells and red blood cells in feces of patients in the treatment group decreased significantly (P < 0.05). The number of plasmodium in peripheral blood of patients in the treatment group was significantly decreased. Conclusion: Wei Chang An Pills combined with Artemether Injection have the good effect in the treatment of malaria (gastrointestinal type), and can improve the clinical symptoms of patients and shorten the course, and also have anti-malarial function.

Abstract: Objective To analyze a case of bloodstream infection caused by Ureaplasma urealyticum after abortion in Anxi County Hospital, so as to provide basis for the clinical diagnosis and treatment. Methods　The diagnosis of Ureaplasma urealyticum in this patient with bloodstream infection was retrospectively analyzed. The basic clinical data and laboratory diagnosis data were collected, including the characteristics of blood culture curve, Wright staining of culture medium, drug sensitivity of Mycoplasma liquid identification, colony characteristics of solid medium, and the conclusion of targeted DNA sequencing. Through the comprehensive analysis of the above data, the rapid diagnosis of this case can be realized by optimizing the detection and diagnosis process. Results　The clinical manifestations of this patient were fever of 38.5 ℃, CRP:14.85 mg/L, WBC:14.33×109/L, NET: 85.40%, PCT: 0.12 ng/mL, IL-6: 665.6 pg/mL, positive after 3 days of blood culture, no bacteria were found in Gram stain, and sand-like purple bacteria were observed after adding Wright's stain. After inoculation in blood agar, Mycoplasma solid and liquid medium, no colonies were grown in blood agar, after 48 h and 5 d. On Mycoplasma A7 agar, the edge of brown fried egg colony was striature, and it could be identified as Ureaplasma urealyticum with the Mycoplasma ID & AST panel, which was resistant to quinolones and spectinomycin, but sensitive to macrolides, tetracyclines and lincomycin. Subsequent targeted DNA sequencing results were also confirmed for Ureaplasma urealyticum. Before receiving the report, clinical experience treatment with ceftriaxone metronidazole was used to fight infection with negative bacilli and anaerobic bacteria. Mycoplasma was not treated with targeted treatment. After 3 days, the patient's body temperature returned to normal, inflammation index decreased, and the patient asked to be discharged. Conclusions　At present, there are few reports of bloodstream infection caused by Ureaplasma urealyticum, and the lack of clinical understanding can easily lead to misdiagnosis and missed diagnosis. In order to improve the detection rate of Mycoplasma in blood culture, it is necessary to optimize the detection procedure of blood culture and provide accurate diagnosis and treatment basis for clinical practice. However, it is clear from this case that Mycoplasma bloodstream infection cases are self-limited infection and can recover by themselves without targeted treatment in patients with normal immunity. Therefore, it is very important to protect the immunity of patients.

Objective To evaluate dialysate exchange procedures of patients on peritoneal dialysis,and analyze the relationship between dialysate exchange skills and dialysis duration. Methods A self-designed evaluation form was used to check the patients' dialysate exchange procedure when they came to the clinic for transfer set change, and the data were analyzed using statistical mehods. Results The global score of assessment was 79. 15 ± 14.87,42.3% patients passed the test. Patients did not have good adherence to Ultraviolet radiation, environment cleaning, hand washing, and masks wearing. The global score for patients on PD more than one yearwas lower than the others (76.52 ± 15.34 vs 87.94 ±8.84, P<0.01 ). The score of preparation procedure, operation process, and global score had significantly negative correlation with dialysis duration(P <0.01). Conclusions PD patients may not adhere adequately to dialysate exchange procedures at home. And this problem may become more sever along with their dialysis duration. Periodically retraining is thus very needed for the long term PD patients.