Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

0.05);the costs were 459.00 yuan,481.68 yuan and 789.48 yuan,respectively,showing significant differences between Group A and Group C or between Group B and Group C(P0.05).CONCLUSION: Group A is preferable in terms of cost-effectiveness,while there was no significant difference between Group A and Group B in cost.

After a description of the connotation and features of ubiquitous information society and the connotation and components of information literacy of medical staff in ubiquitous information society, the approaches were put forward for training and improving the information literacy of medical staff in ubiquitous information society.

This paper reports the observation on the distribution of succinic dehydrogenase of the cochlea in normal guinea pig. The method we adopted is as follows: The temporal bone was rapidly removed and the apical portion of the cochlea and the round window were perforated after decapitation under the temperature at 0? to 4℃. Incubation solution was infused into the apical portion of the cochlea and flowed out through the round window. The above procedure was carried out within 3 min. Then the specimen was put into incubation solution for 30 min at 37 C. The same infusion pathway from apical hole to round window was adopted, to introduce a cold, buffered formol sucrose solution for fixation. After first fixation by infusing, the cochlea in the fixative was subjected to a vacuum system for 20～30 min, and finally put into refrigerator to complete fixation. The specimen was then decalcified in 10 per cent EDTA, and immersed in 10 and 20 per cent gelation successively for embedding. The frozen sections was cut into 15? thick midmodiolar sections in a cryostat. This observation reveals that the inner and outer cell, the cell of spiral ganglion and the stria vascularis showed a very strong enzyme activity, while the tectorial membrane, the basilar membrane and other cells showed no activity. The results obtained and the methods are discussed.

OBJECTIVE:To provide information for diversified operation of Chinese pharmaceutical retail chain enterprises.METHODS:By means of introducing the concept of commodity management,its effective application in Chinese pharmaceutical retail chain enterprises was analysed.RESULTS&CONCLUSION:Chinese pharmaceutical retail chain enterprises should apply commodity management theory to searching the mode of diversified operation,for improving net sales and market competition ability.

OBJECTIVE:To discuss about the management of Chinese pharmaceutical retail commerce METHODS:According to the analysis of the operation models and existing problems of Chinese pharmaceutical retail commerce,this paper brings forward concrete management strategy RESULTS & CONCLUSION:Chinese government and pharmaceutical retail enterprises should adopt effective management strategy to promote development of Chinese pharmaceutical retail commerce

OBJECTIVE:To provide the concept of management of circulation of commodities for Chinese pharmaceutical retail chain enterprises METHODS:According to the concept of management of circulation of commodities,to analyze its application in Chinese pharmaceutical retail chain enterprises RESULTS &CONCLUSION:Chinese pharmaceutical retail chain enterprises should search a mode of management of circulation of commodities which is appropriate for self-development to promote market competition ability

OBJECTIVE: To discuss the measures for development of pharmaceutical retail chain enterprises in China after access to WTO.METHODS: Practical developing measures were put forward by way of analysing the existing problems in pharmaceutical retail chain enterprises in China .RESULTS: & CONCLUSION:China s pharmaceutical retail chain enterprises should take the following measures perfecting relevent laws and regulations, deciding rational organization and clarifying the post responsibility, extending the business scale of chain stores, bringing in qualified personnel and training staff members, en-hencing the management of storage and delivery centre and intensifying the management of chain drugstores.

OBJECTIVE:To provide suggestions for perfecting the laws and rules of drug circulation in China.METHODS:To collect,classify and analyze the current laws and rules for drug manufacturing enterprices,common merchandising concerns,drug wholesale enterprises,drugs retail enterprises and pharmaceutical retail chain enterprises in the field of drug circula?tion.RESULTS&CONCLUSION:We should perfect the current laws and rules,and form the system of law for circulation of drugs applicable for each trade in the field of drug circulation,on the other hand,we should add the rules for chain trans-management,chain affiliation,merger,association,cooperation,drug electronic commerce etc.

The chemical constituents of the gorgonian Isis minorbrachlasta have been studied for the first time. Th?marine specimen was collected off the Nansha Islands in the South China Sea . On the basis of spectral correlation (1H NMR,13C NMR,IR)four known compounds:gorgosterol,22 - epi-hippuristanol, ceramide and batyl alcohol, were indentified.