This study was carried out to investigate the response of interphase Y chromosome to some drugs and toxins by observing the rate of leukocytes showing Y-body in the peripheral blood. The interphase Y chromosomes of blood leukocytes were stained with quinacrine mustard and the animals used were rabbits, rats and guinea pies. Y-bodies of leukocytes were studied in the animals as well as in man. Changes in positive rate of Y-body in leukocytes and total leukocyte count were observed in the rabbits administered with drug or toxin. The results concerning the rate of Y-body were as follows: 1. Y-bodies were present in the blood leukocytes of the animals. However positive rates in mononuclear and polymorphonuclear leukocytes were lower in animals that in man. The positive rate of Y-body was higher in mononuclear leukocytes than in polymorphonuclear leukocytes in the animals as in man. 2. Bacterial toxins such as typhoid, D.P.T. and cholera vaccines and anticancer drugs such as busulfan and endoxan reduced the positive rates of Y-body both in mononuclear leukocytes and in polymorphonuclear leukocytes. 3. Benzene known as bone marrow toxin reduced the positive rate of Y-body in mononuclear leukocytes, but not that in polymorphonuclear leukocytes. Quinine known as general protoplasmic poison reduced the positive rate of Y-body not only in mononuclear leukocytes but also in polymorphonuclear leukocytes. 4. Antibiotics such as tetracycline and chloramphenicol and steroid hormones such as estrogen, testosterone and prednisolone had no effects on the positive rate of Y-body both in mononuclear leukocytes and in polymorphonuclear leukocytes.
Animals ; Anti-Bacterial Agents ; Bacterial Toxins ; Benzene ; Bone Marrow ; Busulfan ; Chloramphenicol ; Cholera Vaccines ; Cyclophosphamide ; Cytoplasm ; Estrogens ; Guinea ; Interphase ; Leukocyte Count ; Leukocytes* ; Leukocytes, Mononuclear ; Neutrophils ; Prednisolone ; Quinacrine Mustard ; Quinine ; Rabbits* ; Rats ; Testosterone ; Tetracycline ; Typhoid Fever ; Y Chromosome

Multiple studies have established that mild encephalitis/encephalopathy with a reversible splenial lesion (MERS) exhibits reversible diffusion restriction in the white matter, including the splenium. There have been a few previous reports of the change in fractional anisotropy (FA) of MERS cases. Herein, we report the longitudinal changes in axial and radial diffusivity (AD and RD), and FA in a 15-year-old boy patient with MERS. Our case demonstrated that a MERS lesion had a significant drop of AD in the early period and gradual recovery. On the contrary, RD did not show any significant change.

Purpose: The aim of this study was to compare the short-term outcomes between laparoscopic liver resection (LLR) and open liver resection (OLR) in elderly patients with hepatic tumors. Methods: From January 2013 to December 2019, a retrospective study was conducted for a total of 143 patients with over 70 years of age, who underwent liver resection for hepatic tumors. Forty-five patients who received biliary reconstruction at the same time were excluded. According to surgical approaches, 98 patients were classified into LLR and OLR groups. All postoperative complications were classified according to the Clavien-Dindo grading system and the Comprehensive Complication Index (CCI). Results: Incidence of the postoperative complications was not statistically different between LLR and OLR groups. The CCI was significantly lower in the LLR group, with a median of 8.556, and a median of 19.698 in the OLR group (p=0.042). The length of hospital stay in the LLR group was significantly shorter than in the OLR group (p=0.008). Conclusion LLR is safe and feasible as a treatment for hepatic tumor in elderly patients with potentially less postoperative complications compared to OLR.

Purpose: : This study aims to identify the factors influencing field adaptation among nurses who have undergone department transfers, by examining the levels of and the relationships between their nursing work environment, interpersonal skills, grit, and field adaptation. Methods: : The study involved 137 nurses who had experienced at least one department transfer within the last three years across two hospitals. Data analysis was performed using SPSS/WIN 29.0, involving descriptive statistics, means, standard deviations, independent t-tests, one-way ANOVA, Scheffé post-hoc tests, Pearson correlation coefficients, and multiple regression analysis (enter method). Results: : Factors affecting field adaptation included the nursing work environment (β=.37, p ＜.001), interpersonal skills (β=.19, p =.021) and grit (β=.18, p =.025), with a total explanatory power of 31% (F=12.16, p ＜.001). Conclusion : This study contributes insights into effective nursing personnel management, offers foundational data for managing department transfers, and aims to improve the overall quality of nursing care.

Cellular oncogenes are expressed as an intrinsic part of the transformed or neoplastic phenotype. More than 60 of the known cellular oncogenes play a specific role in normal cellular development and differentiation. To examine the correlation between ras oncogene expression and the development of cervical cancer, this study investigated the reactivity of cervical intraepithelial neoplasia(CIN) and carcinoma of the uterine cervix by using anti-ras P21 mouse monoclonal antibody. The expression of ras oncogene significantly increased with the grade of malignancy from 11% in severe dysplasia, 30% in carcinoma in situ, 43% in microinvasive carcinoma, to 53% in invasive cancer. The expression of ras oncogene was not correlated with histologic type, tumor size, and nodal status of cervical cancer. It was concluded that expression of ras oncogene is related to early phase of carcinogenesis and tumor invasion of carcinoma of the uterine cervix.
Animals ; Carcinogenesis ; Carcinoma in Situ ; Cervix Uteri* ; Female ; Genes, ras* ; Mice ; Oncogenes ; Phenotype ; Uterine Cervical Neoplasms

PURPOSE: To compare results between in femtosecond laser and microkeratome LASIK correction of myopia METHODS: We retrospectively analyzed the result of 94 eyes of 47 patients in the femtosecond group (F) and 103 eyes of 52 patients in the microkeratome group (M). All patients had undergone LASIK using either a femtosecond laser or a microkeratome for making of flap. Patients were divided into groups I (6D< or =) and II (> or =6D) according to preoperative myopia. Each patient was followed up for over 6 months with measurements of uncorrected visual acuity and manifest refraction at 1 week and 1, 3, and 6 months after operation. Complications during and after the operation were reviewed retrospectively in two groups 6month after the operation. RESULTS: In groups F-I, F-II, M-I, and M-II, postoperative 6-month uncorrected visual acuity was 0.98+/-0.08, 0.96+/-0.09, 0.97+/-0.03, 0.98+/-0.09. At the 6-month follow-up, there were no significant differences between the two groups in uncorrected visual acuity and mean spherical equivalent. Corneal opacity was found in 3 eyes in group M and complication related with flap was found 1 eye in group F and 4 eyes in group M. CONCLUSIONS: During a 6-month follow up, LASIK using either a femtosecond laser or a microkeratome has an similar effect in uncorrected visual acuity and mean spherical equivalent. Thus LASIK for using femtosecond laser can be used as an alternative procedure to correct myopia.
Corneal Opacity ; Follow-Up Studies ; Humans ; Keratomileusis, Laser In Situ* ; Myopia* ; Retrospective Studies ; Visual Acuity