Cardiopulmonary Resuscitation ; adverse effects ; Chylothorax ; etiology ; Heart Arrest ; physiopathology ; Humans ; Infant ; Infant, Newborn ; Male ; Neonatology

To establish a method for the determination of the content of total flavones and the quality control of Runchang Ningshen Extract. [Methods & Results]With rutin as control,the optimal concentration of Runchang Ningshen Extract was obtained by refluxing for three times with 70% ethanol.Ultraviolet spectrophotometry was used.The regression equation was induced at detecting wavelength being 511nm and with sodium nitrite and aluminium nitrate as color developing agent.There was a good linearity (r=0 9999)in the range of 8 ?g.The average recovery of total flavones was 94 63%(RSD=2 58%,n=5).[Conclusion]This method is simple and reproducible and can serve as the standard for controlling the quality of Runchang Ningshen Extract.

Basic fibroblast growth factor(bFGF) is one of the most important factors for wounds healing.BFGF promotes healing of bone fracture by regulating cell proliferation and differentiation of bone tissues,increasing local bone density,and accelerating local angiogenesis.With the progression of bFGF research,more and more attention will be paid to bone repair function of bFGF in bone tissue engineering.

117 guinea pigs were divided into groups, receiving 1mg, 5mg, 50mg and 100mg Vit C per os daily respectively in 2-4 weeks. There were conducted four experiments: (1) the effect of different vitamin C levels on the cold tolerance and adaptation in guinea pigs; (2) effect of large dose of vitemin C on sudden and long time exposure to cold: (3) the action of vitamin C, in large dose and short time in combination with hormones, on cold tolerance of guinea pigs; (4) effect of large dose of vitamin C on the myocardial enzyme activity, during the process of cold adaptation. Rectal temperature, body weight, weight and vitamin C contents of adrenal glands, the excretion of urinary 17-Ketostero'ids, activity of myocardial succinic dehydrogenase and cytochrom C oxidase were observed. The results revealed that large dose of Vitamin C per os might lessen the lowering of rectal temperature and body weight after exposure to cold; promote the activities of myocardial enzymes. Better results were obtained with combination of vitamin C with cortisones. It was noted that large dose of vitamin C apparently increased and accelerated the tolerance and adaptation of organisms to cold.

The dietary composition of inhabitants of the endemic and nonendemic areas, the epidemics of Keshan disease, and its clinical examinations from 14 counties in Heilongjiang, Hebei, Shanxi, Shantong, Shanxi, Sichuan, Yunnan and Xizhang provinces were investigated.The results revealed: (1) There were differences of the dietary sources between endemic and non-endemic inhabitants (difference of water-soil conditions); (2) The composition of diet between inhabitants of endemic and non-endemic areas were different; (3) There were differences of dietary composition of the inhabitants between peak year of incidence and ordinary year in endemic districts. The less the variety of diet the higher the incidence of the disease; the greater the variety of diet, the lower its incidence. The decrease of the incidence was closely related to the improvement of dietary conditions in endemic area.

Lithospermum has been widely used in clinic for a long time. It can lower the levels of follicle stimulating hormone and luteinizing hormone in blood serum and inhibit ovulation, thus causing infertility. Due to its effect of lowering chorionic gonadotropin, restraining the development of corpus luteum graviditatis and interfering the growth of uterus and the supply of embryotrophy, Lithospermum has been confirmed to be effective in termination of pregnancy and herb abortion. Therefore Lithospermum can not be used in those who intend to conceive or do not need to terminate pregnancy. The authors suggest that the influence of Lithospermum on pregnancy should be studied objectively and should be emphasized in clinical teaching of traditional Chinese medicine to ensure the correct and reasonable application of Lithospermum.

Statins not only can help lower cholesterol,but also has certain effect for the treatment of malignant tumor.Its possible antitumor mechanisms include inducing apoptosis and differentiation,inhibiting tumor cell proliferation,reducing the invasion and metastasis of tumor cells,combined sensitization and chemical prevention effect.

In recent years the loss of humanity spirit in medicine has been the undeniable fact,which accordingly pro-vokes criticisms from patients and media.To reshape the perfect image of medical circle,it is pressing to transcend theutilitarianism of medical education , enhance the humanity education and develope the spirit of humanity.

Objective To establish a multiplex polymerase chain reaction (PCR) assay for simultaneous identification of Shigella spp., Salmonella spp. and Vibrio cholerae. Methods Based on the gene sequences of invasion plasmid antigen H (ipaH) in Shigella spp., invasion plasmid antigen B(ipaB)in Salmonella spp. and enterotoxin extracellular secretion protein (EPSM) in V. cholerae, three pairs of primer were designed. Genomic DNA was extracted by the boiling method and multiplex PCR was performed with premix Taq in an ABI 2720 thermal cycle. The PCR-amplified products were then analyzed by using agarose gel electrophoresis. Results Under the optimized conditions, the assay yielded a 606-bp product from Shigella spp., a 314-bp product from Salmonella spp., and a 482-bp product from V. cholerae, respectively. When the DNA extraction of multiple target organisms was included in the same reaction, two or three corresponding amplicons in different size were observed. Conclusions A rapid, specific and sensitive multiplex PCR system for simultaneous detection of Shigella spp., Salmonella spp. and V. cholerae has been established. The results suggest that the simultaneous amplification of several genes by multiplex PCR may provide an efficient and rapid diagnostic method for severe diarrhea.

Th17 lymphocytes have been recently identified as a novel subset of CD4+ cells. It has been defined that IL-17,the main product of Th17, plays an important role in immunity against parasitic infection. There is a two-way influence between Th17 and cytokine network: on one hand Th17 consummates cytokine network, on the other hand many cytokines regulate Th17's activity in parasitic infection. In the anti-parasitic infection process, Th17 cells protect host or promote inflammation, even cause immune pathogenesis in different cases, which comprise host's immune state, the burden of parasitic infection, as well as the treatment.