[Summary] Cardiovascular disease ,diabetic nephropathy and diabetic retinopathy are common complications of diabetes ,and are the significant reason of cardiovascular mortality ,end-stage renal disease ,and blindness in adults patients with type 2 diabetes mellitus. At present, the researche on the pathogenesis of diabetic complications mainly focus on oxidative stress and chronic inflammation. In addition, a large number of studies have pointed out that bilirubin has antioxidant and anti-inflammatory effects. Therefore, bilirubin and diabetic complications are closely linked.

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

BACKGROUND:The tissue structure of the affected arms of the breast cancer patients is seriously damaged after operation, which often causes the disorder of functional activity and directly affects the lives and work of Breast Cancer patients.OBJECTIVE: To promote the functional recovery of affected-side upperlimb in patients with breast cancer after operation by three-stage functional training and health guidance.DESIGN: Clinical observationSETTING: Department of Nursing, First Affiliated Hospital, China Medical UniversityPARTICIPANTS:Totally 168 patients,to be all female,aged from34 to72 years with the average of 49.65 years, diagnosed breast cancer and received operation in the First Affiliated Hospital of the China Medical University from May 2002 to May 2004, were recruited.METHODS:Self-training was performed on the upper-arm at the affectedtion ( one or two days after operation): Do active and passive exercises of Off-bed stage (3 to 14 days after operation): Do exercises of shoulder joint,hospital( 3 to 6 months), functional exercise and guidance training on the upper arm, 2 to 3 times per day , 15 to 20 minutes per time. Self-training effect of the patients was investigated through further consultation, once every 2 months after the self-training.cises status and the abnormal status of their affected arms of the patients.RESULTS :Totally 168 patients completed the study,all their date was enbetter recovery with their affected arms and could do some simple work 75.0% (126/168)of the patients persisted in the functional training of their affected arms over half a year after they discharged from the hospital;25.0% (42/168)of the patients had different degrees of lymphatic edema of the affected arms.CONCLUSION:We have got an obvious effect in the aspects of strengthening the guidance of the functional training of their affected arms of the breast cancer patients and telling the patients and their family members the significance of persisting in training their main points of self nursing of their affected arms.

Connection between the superior colliculus(SC)and the dorsal and ventral lateral geniculate nuclei (LGd and LGv) were studied in adult golden hamsters using anterograde and retrograde pathway tracing methods 1. In four hamsters, the cytoarchitecture of the SC, LGd and LGv was examined in brain sections stained with Nissl's or Loyez's method. 2. A single injection of a mixture of ~3H-leucine and ~3H-proline was made into various regions of the SC in each of 4 animals (1 day survival) for studying the pattern of terminal distribution of the projections from the SC to the ipsilateral LGd and LGv. In animals where the injection site was located in the superficial gray layers of the lateral portion of the SC, labelled terminals were found in the caudal lateral part of the LGd and LGv. In the case where the injection was restricted in more medial part of the SC, the terminal labelling was observed in the rostral lateral portion of the lateral geniculate nuclei. 3. One day after an injection of horseradish peroxidase (HRP) into the LGd (4 animals), LGv(2 animals), or LP(2 animals) labelled neurons were observed in the superficial gray layers of the ipsilateral superior colliculus. No HRP-labelled neuron was detected in deeper laminae in the colliculus. These results indicate that neurons in the superficial gray layers of the SC project to the ipsilateral LGd and LGv, and the projections are organized topogra- phically according to the retinotopic or visual field map

This paper reports the observation on the distribution of succinic dehydrogenase of the cochlea in normal guinea pig. The method we adopted is as follows: The temporal bone was rapidly removed and the apical portion of the cochlea and the round window were perforated after decapitation under the temperature at 0? to 4℃. Incubation solution was infused into the apical portion of the cochlea and flowed out through the round window. The above procedure was carried out within 3 min. Then the specimen was put into incubation solution for 30 min at 37 C. The same infusion pathway from apical hole to round window was adopted, to introduce a cold, buffered formol sucrose solution for fixation. After first fixation by infusing, the cochlea in the fixative was subjected to a vacuum system for 20～30 min, and finally put into refrigerator to complete fixation. The specimen was then decalcified in 10 per cent EDTA, and immersed in 10 and 20 per cent gelation successively for embedding. The frozen sections was cut into 15? thick midmodiolar sections in a cryostat. This observation reveals that the inner and outer cell, the cell of spiral ganglion and the stria vascularis showed a very strong enzyme activity, while the tectorial membrane, the basilar membrane and other cells showed no activity. The results obtained and the methods are discussed.

Objective To realize the automatic conversion of material management information to branch-level material cost accounting. Methods According to the structure of material management system and cost accounting system, memory procedure of HIS was set to undertake the mission of information conversion. Results Automatic conversion of material management information to branch-level material cost accounting saved a lot of manpower and raised the efficiency and accuracy of cost accounting. Conclusion This solution is worth popularizing not only in other cost accounting but in information system integration and data sharing.

Objective To construct a computerized medical case archive system, which can realize the electronic storage of medical case. Methods Virtual print was used in archive computerized medical cases and electronic signature was used to ensure the legality of the archives. Results The system realized the patient-centered medical information integration and raised the level of medical information storage and management significantly. Conclusion The favorable foundation was established fully in the medical cases archiving system, but the system can be improved in future.

Objective:To compare the therapeutic effect of drum room injection and intramuscular injection of rat nerve growth factor on occupational noise-induced deafness (ONID).Methods:Sixty-six patients with ONID treatment from January 2018 to December 2019 in the People′s Hospital of Liaoning Province were divided into control group and study group by random number table, with 33 patients in each group. On the basis of conventional treatment, all patients in the study group were injected with 20 μg rat nerve growth factor in the drum room, and each ear was injected once every other day. The control group was given intramuscular injection of rat nerve growth factor 20 μg per day, and the patients in two groups were treatment for 28 d. The hearing threshold of left and right whisper frequency of the two groups were compared. The improvement of tinnitus, dizziness, headache, insomnia, other symptoms and the scores of Hamilton Depression Scale (HAMD) was compared between the two groups.Results:Before treatment, the hearing thresholds of left whisper frequency and right whisper frequency in two groups had no significant differences ( P>0.05). After treatment, the hearing thresholds of left whisper frequency and right whisper frequency in the study group were significantly lower than those in the control group: (23.8 ± 4.2) dB/HL vs. (34.5 ± 3.9) dB/HL, (23.2 ± 4.4) dB/HL vs. (35.9 ± 5.1) dB/HL, and there were statistical differences ( P<0.05). After treatment, tinnitus and dizziness symptom improvement rate in the study group were higher than those in the control group: 93.3% (28/30) vs. 72.4% (21/29), 15/17 vs. 8/16, and there were statistically significant differences ( χ2 = 4.584 and 5.705, P<0.05). There was no statistically significant difference in the improvement rate of headache and insomnia symptoms between 2 groups ( P>0.05). The total effective rate in the treatment group was significantly higher than that in the control group: 90.9% (30/33) vs. 69.7% (23/33), and there was statistical difference ( χ2 = 4.694, P<0.05). After treatment, the scores of HAMD of 2 groups were significantly decreased, and the scores of HAMD in the study group was lower than that in control group: (8.46 ± 3.13) scores vs. (10.55 ± 3.83) scores, and there was statistical difference ( P<0.05). The incidence of total adverse reactions between 2 groups had no significant difference ( P>0.05). Conclusions:The injection of mouse nerve growth factor into drum room to treat ONID can effectively improve the hearing level of patients, alleviate tinnitus and dizziness symptoms and improve adverse mood without obvious adverse reactions.

The chemical constituents of the gorgonian Isis minorbrachlasta have been studied for the first time. Th?marine specimen was collected off the Nansha Islands in the South China Sea . On the basis of spectral correlation (1H NMR,13C NMR,IR)four known compounds:gorgosterol,22 - epi-hippuristanol, ceramide and batyl alcohol, were indentified.

0.05);the costs were 459.00 yuan,481.68 yuan and 789.48 yuan,respectively,showing significant differences between Group A and Group C or between Group B and Group C(P0.05).CONCLUSION: Group A is preferable in terms of cost-effectiveness,while there was no significant difference between Group A and Group B in cost.