Objective To investigate the effects of Pulsatilla saponin D and sorafenib on the metastasis of human hepa?toma cell line. Methods The human hepatoma cell line BEL-7402 cells were divided into Pulsatilla saponin D group (con?centration of 11.9 mg/L), sorafenib group (concentration of 2.15μmol/L), the combined group (Pulsatilla saponin D 11.9 mg/L+Sorafenib 2.15μmol/L) and the control group (ordinary broth). The inhibition effects of Pulsatilla saponin D and sorafenib monotherapy and combination therapy on BEL-7402 cell migration were detected by MTT assay, Transwell chamber experi?ment and cell scratch experiment. Western blot assay was used to detect the expression levels of matrix metalloproteinase (MMP)-2 and MMP-9 gene protein. Results MTT assay showed that Pulsatilla saponin D (11.9 mg/L), sorafenib (2.15μmol/L) monotherapy and combination therapy had inhibitory effects on BEL-7402 cell proliferation, and the 24-h inhibi?tion rate was<15%. Results of Transwell chamber experiment and cell scratch test showed that the migration inhibitory rate was significantly higher in combination group than that of monotherapy group (P<0.01). The combined effect of madicine was the addition (0.85≤Q≤1.15). Western blot detection showed that there was a higher effect of down-regulation on MMP-2 and MMP-9 in combined group than that of monotherapy group. Conclusion Pulsatilla saponin D and sorafenib synergis?tically inhibit the metastasis of BEL-7402 cells. The joint effects are superior to monotherapy.

Orexins are a class of important hypothalamic neuropeptides,including type A and B. Orexins are associated w ith numerous physiological functions, including sleep-aw akening, energy balance, endocrine and visceral functions, and they also have certain relations w ith the pathophysiological changes, such as drow siness and drug abuse. In recent years, the pathophysiological role and mechanism, as w el as the clinical significance of orexins in cerebrovascular diseases are causing concern. This article summarizes the roles of orexins and focuses on the roles of orexin A in cerebrovascular diseases.

Repair of articular cartilage injury has always been a focus of medical study and sports injury study.With the application and development of molecular biotechnology,the role of growth factor has become more and more important in articular cartilage injury.This paper analyzes the difficulties in repairing articular cartilage injury.discusses the effect of transforming growth factor β1 and bone morphogenetic protein-2 on it as well as the mechanism under its repainng,and summanzes the existing problems.it can provide important data for future research.

OBJECTIVE:To elucidate the role of transforming growth factor-β and bone morphogenetic protein-7 in the reparation of knee cartilage by summarizing related studies,which can provide an important reference for further clinical applications.DATA SOURCES:The science online,ElsecierSD databases,Springer Link electronic joumals nets(1991-01/2009-06)was searched using key words of"Articular Cartilage Defects,Transforming Growth Factor-β,Bone Morphogenic Protein-7";simultaneously,the CNKI,Wanfang database,Tsinghua Tong Fang database(1991-01/2009-06)was searched with the same Chinese key words.Literature search was limited to English and Chinese languages.DATA SELECTION:Literature addressing repairing articular cartilage damage with growth factors was included,and the repeated papers were excluded.MAIN OUTCOME MEASURES:①Frecture healing.②Osteocyte proliferation.③Capacity of chonddfication.RESULTS:Received 95 computers seized in early literature,according to inclusion exclusion criteria,literature underlying growth factor,in particular the growth factor transforming growth factor-β and bone morphogenetic protein-7 in repaidng knee cartilagedamage was analyzed.Articular cartilage injury,with poor repair capacity,is more common in athletes.As soon as a permanent injury that generates lesions,it is difficult to treat by traditional treatment methods,which need to be solved in sports medicine.Transforming growth factor-β,an important factor regulating the formation of cartilage,stimulates or inhibits a variety of cells.By increasing the sensitivity of chondrocytes,transforming growth factor-β plays a central role in the process of repairing osteoarthdtis cartilage injury,regulates in vitro protein synthesis,but also affect on the induction of specific granulation tissues.Bone morphogenetic protein-7 can induces cartilage-specific collagen and mucin production by mesenchymal and wound areas,which has promotive effect on cartilage reparation.CONCLUSION:Transforming growth factor-β or bone morphogenetic protein-7 has certain effect on knee cartilage injury;however,whether the combination of them can promote reparation of articular cartilage injury needs to be explored.

Multiple myeloma (MM) is a malignant tumor of plasma cells that remains incurable.More attentions have been lately directed to the immunotherapy,which has proven benefits in eradicating minimal residual disease of MM,reducing relapse and improving patients' overall survival.Mucin 1 (MUC1) is a tumor associated antigen of MM,and has attracted increasing interest as a potential target for MM immunotherapy.In addition,MUC1-based vaccines have quickly entered human clinical trials,and some promising responses have been reported.Here,an up-to-date review of MUC1-based immunotherapy of MM is given.

950 mg/d) group was significantly higher than those in median or low calcium intake group,among girls with BB genotype. However,such significant effect was not detected among girls with b allele. Conclusion:The PTH BstBⅠ polymorphism has significant interaction effect with calcium intake on bone mass accretion in Chinese adolescent girls. High calcium intake would be beneficial to gain higher bone mass accretion for girls with BB genotype than those with b allele.

In order to promote rehabilitation exercises for breast cancer patients and to improve their quality of life,as well as to promote the development of nursing profession, our hospital has estab-lished a specialistnursing clinic for breast cancer since January 2016. We standardized nurses' qualification and training requirements, and performed management in patients'symptom assessment, nursingtechniqueguidance for breast cancer, sleep guidance, health education, long-term follow up, etc. After one year of implementation, symptom assessment was conducted 1283 times, rehabilitationexerciseguidance was 329 times, lymphatic drainage guidance was 297 times, sleep guidance was 159 times, health education was 591 times, sexual life with spouse guidance was 107 times, in-hospital nursing consultation was 48 times, family on-site demonstration was 29 times. The satis-faction rate was 98.4% which was higher than average level of the hospital according to outpatient investigation.

Objective To investigate clinical value of single nucleotide polymorphism array (SNParray) gene chip technique in diagnosis of genetics of recurrent spontaneous abortion (RSA).Methods From January to October 2012,the 26 patients with more than twice of spontaneous abortion in Huzhou Maternal and Child Health Care Hospital were enrolled in this study(RSA group).Meanwhile 20 cases with induced abortion were taken as control group.All aborted tissues were analyzed with conventional cytogenetic karyotyping and SNP-array,respectively.Results Chorionic villus chromosomal examination was successfully done in 19 cases (73％,19/26),which 10 cases were found with chromosomal anomaly,the overall detection rate is 10/19.However,SNP-array analysis was successfully performed in all 26 cases.The overall rate of detection was 100％,and abnormalities were found in 15 cases,which reached the detection rate was 58％ (15/26).Chorionic villus chromosomal examination was successfully done in 16 cases (16/20) in control group,and none of the resluts was chomosomal anomaly; SNP-array analysis was successfully performed in all 20 cases (20/20),and none was found abnormalities.Conclusion SNP-array gene chip technique showed highly accurate feature,which could be used in cytogenetic diagnosis of recurrent spontaneous abortion.

Objective To determine the incidence of thrombogenic events during the prostate operation period in patients discontinuing aspirin.Methods Among a retrospective cohort of 342 patients admitted in our institution for benign prostatic hyperplasia (or prostate cancer),combined with acute coronary syndrome (or stroke),we studied 4 patients who had not been taking aspirin before thrombogenic vascular event.Data on age,sex,vascular disease risk factors,and clinical outcome were collected.Results The 4 patients' mean age was 78.8±5.9 years.Each patient had at least two following risk factors:atrial fibrillation,old cerebral infarction and type 2 diabetes.80％ patients had a clinical history of hypertension.2 of the 4 patients stopped aspirin before a surgical procedure and developed acute ischemic stroke and acute myocardial infarction,separately.The other two patients developed acute ischemic stroke without aspirin prescription.The median time between admission and thrombogenic events was 15.5± 10.5 days.All patients were not given finasteride on admission.Conclusions This study should alert clinicians to know the risk of aspirin withdrawl perioperatively in patients at high risk of cardiovascular and cerebrovascular diseases.