It is important and imminent that bilingual teaching in anesthesiology is carried out and promoted in Chinese medical universities.This teaching has been implemented in Chongqing Medical University for ten years.The desired results have been achieved.This paper analyzes the importance of proper attitudes towards bilingual teaching,using the efficient methods,paying more attention to developing the students' ability of autonomous and improving the teachers' education quality.

Objective: To determine the effect of K ATP on the reduction of blood pressure variability (BPV) caused by adenosine in conscious, freely moving sinoaortic denervated (SAD) rats. Methods: Using computerized analytic system of blood pressure (BP), five groups treated with adenosine, adenosine A 1 receptor agonist N 6 cyclopentyladenosine (CPA), A 2 receptor agonist 5′ N cyclopropyl carboxamido adenosine (CPCA), ATP sensitive K + channel (K ATP ) blocker glibenclamide, and CPCA with the K ATP blocked by glibenclamide in advance were used. BP, heart period (HP) and BPV were analyzed. Results: Both adenosine and CPCA significantly decreased BPV in SAD rats, but CPA had no influence on it. Glibenclamide markedly reduced BPV in SAD rats as well, furthermore, it might antagonize the effect of CPCA on BPV by blocking K ATP . Conclusion: K ATP plays an important role in the development of high BPV in SAD rats. [

Objective To investigate clinical value of single nucleotide polymorphism array (SNParray) gene chip technique in diagnosis of genetics of recurrent spontaneous abortion (RSA).Methods From January to October 2012,the 26 patients with more than twice of spontaneous abortion in Huzhou Maternal and Child Health Care Hospital were enrolled in this study(RSA group).Meanwhile 20 cases with induced abortion were taken as control group.All aborted tissues were analyzed with conventional cytogenetic karyotyping and SNP-array,respectively.Results Chorionic villus chromosomal examination was successfully done in 19 cases (73％,19/26),which 10 cases were found with chromosomal anomaly,the overall detection rate is 10/19.However,SNP-array analysis was successfully performed in all 26 cases.The overall rate of detection was 100％,and abnormalities were found in 15 cases,which reached the detection rate was 58％ (15/26).Chorionic villus chromosomal examination was successfully done in 16 cases (16/20) in control group,and none of the resluts was chomosomal anomaly; SNP-array analysis was successfully performed in all 20 cases (20/20),and none was found abnormalities.Conclusion SNP-array gene chip technique showed highly accurate feature,which could be used in cytogenetic diagnosis of recurrent spontaneous abortion.

"CapFinder" technology,which can be used to clone the full length of 5′ UTR sequence of mRNA,was described.This technology used the terminal transferase activity of certain MMLV RT variants that added 3-5 residues(predominantly dC) to the 3′end of the first-strand cDNA exhibited when MMLV RT reached the 5′cap structure of mRNA.In the reverse reaction system containing GGG oligo,the terminal transferase activity was harnessed by the GGG oligo whose terminal stretch of dG residues can anneal to the dC-rich cDNA tail and serve as an extended template for RT.After RT switch templates from the mRNA template to the GGG oligo,a complete cDNA copy of the original RNA was synthesized with the additional GGG oligo sequences at the end.5′UTR of mRNA can be amplified with GGG oligo as forward primer and a gene-specific reverse primer.5′UTR of Bt toxin receptor E-Cadherin gene in midgut of cotton bollworm was cloned.

Objective To evaluate the effect of controlled decompression on complications such as delayed intracranial hematoma,acute encephalocele and cerebral infarction in patients with severe traumatic brain injury.Methods Pubmed,Cochrane Library,Wanfang data,VIP and CNKI wcrc searched for related literaturc about controlled decompression (treatment group) and traditional surgical methods(control group) for severe traumatic brain injury.The data that met the inclusion criteria were extracted,and analyzed statistically using the Review Manager 5.3.Results A total of 12 studies were included in this meta-analysis,with respective 730 patients in control group and 908 patients in treatment group.Controlled decompression versus traditional treatment methods reduced incidence of delayed intracranial hematoma (RR =0.55,95％ CI 0.44-0.70,P ＜ 0.01),acute encephalocele(RR =0.42,95％ CI 0.32-0.53,P ＜ 0.01) and cerebral infarction (RR =0.42,95％ CI 0.32-0.55,P ＜0.01).Conclusion Applied to treat severe traumatic brain injury,controlled decompression exhibit significantly lower rate of delayed intracranial hematoma,acute encephalocele and cerebral infarction than traditional methods.

According to the demand of combat readiness,a high-speed material handling machine based on field conditions is designed and manufactured to enhance the reaction and mobility of Mobile Medical Team.It is confirmed by the practice that this machine has such advantages as high efficiency,safety,convenience and easy maintenance.The difficulty of material handling in field conditions is solved.

Objective To identify applied value of multifunctional peritoneal biopsy needle(MPBN)in the diagnosis of tuberculous peritonitis(TP).Methods Peritoneal biopsy was performed with MPBN in 19 cases of 27 patients with TP between January 2001 and September 2005,which was compared with the clinical presentation,laboratory analysis and laparoscopy.Results Five patients were diagnosed as TP according the clinical presentation and laboratory analysis.In 19 patients with TP,73.68% was accurately diagnosed as with MPBN.All of the 3 patients were accurately diagnosed as TP with laparoscopy.Conclusion Peritoneal biopsy with MPBN is useful in diagnosis of tuberculous peritonitis,especially when there is no laparoscope.

Objective To assess the value of implanting remedial or prophylactic cardioverter defibrillator in patients with Brugada syndrome for protecting from malignant ventricular arrhythmias and to estimate the initial outcomes in one-month follow-up. Methods Implantable cardioverter defibrillators (ICDs) were implanted in 21 consecutive patients (9 symptomatic and 12 asymptomatic) with Brugada syndrome using standard procedure under general anesthesia. Patients presented the typical ECG changes of Brugada syndrome with or without sodium channel blockers but without structural heart disease in all the cases. Electrophysiological examinations were performed in all patients. Eight asymptomatic patients had spontaneous or induced malignant ventricular arrhythmias and 4 asymptomatic patients had single positive family history. Results Sixteen single chamber ICDs (VVI) and 5 double chamber ICDs (DDD or DDDR) were implanted. The DDD- or DDDR-ICDs were applied for patients with sinus node dysfunction and/or atrioventricular conduction disturbances. Battery/lead status measurements of ICDs were good and met with standards of implantation during the procedure. No complications occurred after ICD implantation and also no changes of various electrophysiological parameters during re-hospitalization in one-month follow-up. There were no events' record of anti-tachycardia pacing or cardiovertion or defibrillation for ventricular arrhythmias in the memory of treatment by ICDs. Conclusions ICDs were implanted successfully with safety in patients with Brugada syndrome showing good function for the initial outcomes in one-month follow-up.

Objective To observe the clinical efficacy of reinforcing-reducing needling methods in treating peripheral facial paralysis. Method Seventy facial paralysis patients were randomized into two groups to compare the reinforcing-reducing needling methods and conventional acupuncture. The House-Brackmann (H-B) scale was observed and compared prior to the treatment, right after the treatment, and respectively after 30-day, 3-month, and 6-month treatments, and the clinical efficacy was evaluated after 6-month treatments. Result The H-B score of the reinforcing-reducing manipulation group was superior to that of the conventional group (P<0.05), and the therapeutic efficacy of the manipulation group was more significant than that of the conventional group (P<0.05). Conclusion The reinforcing-reducing needling methods can produce a better therapeutic efficacy in treating peripheral facial paralysis compared to conventional acupuncture.

In order to decrease the mortality of pre-hospital sudden death events(SDEs),their features especially those of cardiac sudden death were analyzed. 1030 pre-hospital emergent cases from 1973～1999 were analysed systematically. The total SDEs occurred in 106 cases, among the 68 cases of cardiovascular diseases, 89.7% had coronary heart diseases(CHD), AMI or OMI with re-infarction. There were two peak age stages of SDEs: the first was 46-55 years old stage(non-retaired age stage,22.6%), and the second stage was 66-75 years old(27.3%). The pre-hospital SDEs are the most emergent cases. Great attention must be paid to those with the history of cardiovascular diseases, especially those with the history of coronary cardiac events