To enforce the application of medical techniques in the primary hospital so as to improve clinical quality and ensure clinical safety, we established the evaluation criteria for admittance of medical techniques class Ⅰ and the incentive system for new technique application. When both systems were applied, favorable results were obtained.

Total 149 strains of endophytic bacteria were isolated from different healthy organisms of Glycyrrhiza inflata Bat. from Xinjiang. The fermented liquids of these strains were screened by TLC and analysed by HPLC, and the glycyrrhizinic acid monoammonium salt was taken as standard control at the same time. Only one endophtytic bacterial stain of Bacillus subtilis could produce Glycyrrhizinic acid monoammonium salt analogue.

Conidial suspension was obtained from the Penicillium sp. DS9713a which could decompose PHB. Through screening a lot of mutants with the methods of transparent zones and flask culture, five strains were selected from starting strain treated with UV and their activity were higher than the original .The best mutant strain named DS9713a-CS01, it's enzyme activity was as 197.42% high as that of the original ,furthermore, the characters of crude enzyme were studied.

Objective To determine the reliability of Chinese- version of Cerebral Palsy Quality of Life Questionnaire for Children (CPQOL) between self-raters. Methods Family members of 21 children with cerebral palsy from rehabilitation centers or hospitals, 2 members of the same one, and 18 teenagers with cerebral palsy from a special school along with one of their parents, answered CPQOL. The intraclass correlation coefficient (ICC) between family members, as well as teenagers and their parents were calculated, and the differences of scores of each dimension between parents and teenagers were compared. Results The reliability between family members was acceptable (ICC=0.49-0.86) in all the dimension of CPQOL, which was good in the function and acquired service dimensions, and was moderate in participation and body health dimensions. The reliability was weaker between parents and teenagers (ICC=0.29-0.73), which was acceptable in the dimensions of function, and participation and body health; and very low in the dimension of welfare and acceptance. The teenagers often rated better than their parents did, especially in the dimension of participation and body health (P<0.05). Conclusion The reliability of CPQOL is acceptable when rated by family members of cerebral palsy children, especially in the dimensions of function and service acquired. It is weaker between parents and children, especially in the dimension of welfare and acceptance.

Objective To compare the value of conventional ultrasound,hydrosonography and double-contrast enhanced ultrasound in the display capacity of periampullary carcinoma and its relationship with the surrounding tissue.Methods A total of 18 patients with the periampullary carcinoma were diagnosed pathologically or by endoscopic biopsy.Each patient had three imaging modalities by conventional ultrasound,hydrosonography and double-contrast enhanced ultrasound.The display capacity and the relationship between the mass and surrounding tissue were compared with three modalities.Results The display rates of limpid visibilities of carcinoma on conventional ultrasound,hydrosonography and double-contrast enhanced ultrasound were 16.7%(3/18),22.2%(4/18) and 94.4%(17/18),respectively;The display rates of duodenum protrusion on three modalities were 0(0/18),38.9% (7/18) and 72.2% (13/18),respectively,with significant differences among conventional ultrasound,hydrosonography and double-contrast enhanced ultrasound.The double-contrast enhanced ultrasound showed two cases of peripheral vascular invasion and one case of intrahepatic metastasis.Conclusion The double-contrast enhanced ultrasound can increase the display capacity of periampullary carcinoma as a new diagnostic modality for periampullary carcinoma.

Objective To evaluate the influence of two different education approaches on diabetic knowledge of type Ⅱ diabetes.Methods 79 type Ⅱ diabetes cases were collected from March 2010 to May 2010,and divided into experimental group and control group according to admission time.Experimental group was those in the first-half,and received figure dialogue education.Control group was those in the last-half,and received traditional heath education.Diabetes knowledge assessment questionnaire was used to evaluate knowledge level before and after education.Results Knowledge level was higher after education than that before education in both groups.No differences was found between the two groups ( P ＞ 0.05 ).All subjects in experimental group thought figure education could improve the study interesting,68.3％ subjects thought mastering knowledge about diabetes was useful,and 80.0％ subjects expect discussion by group.However,74.4％ subjects in control group thought traditional education could improve the study interesting,15.4％ subjects thought mastering knowledge about diabetes was useful,and 46.2％ subjects expect discussion by group.Conclusions Compare with traditional education,figure education although could not increase the knowledge level about diabetes significantly,but could highly increase the interesting of study.

Objective To assess the quality of life in patients with gynaeeological tumors and provide basis for clinical nurse.Methods 80 patients were investigated by the World Health Organization' s Quality of Life Questionnaire-Brief Version (WHOQOL-BREF) before and after operation.Results The patients had significantly lower scores in physiological and social relationship domains than the Chinese norms [(10.55 ±3.52) vs( 15.10 ± 2.30),( 10.93 ± 1.72) vs ( 13.93 ± 2.06 ) ;t =15.40,12.13,respectively; P ＜ 0.01 ].The patients had higher scores in environmental domain than the norms [(25.93 ± 3.93 ) vs ( 12.14 ± 2.08 ) ; t =49.07 ;P ＜0.01 ].No difference was found in scores in psychological domain between the patients and the norms (P＞0.05).No differences were found in the scores of all four domains among different types of gynaecological tumors (P＞0.05).Conclusions The quality of life will be damaged after suffering from gynaecological tumors,clinical nurses should improve self ability to promote patient rehabilitation and quality of life.

Background:Due to absence of visible endobronchial target,the diagnostic yield of flexible bronchoscopy for peribronchial lesions has been unsatisfactory.Convex probe endobronchial ultrasound (CP-EBUS) has allowed for performing real-time transbronchial needle aspiration (TBNA) of enlarged hilar and mediastinal lymph nodes and therefore could also be used as a means of diagnosing proximal peribronchial lesions.Methods:We retrospectively analyzed the results related to 72 patients who underwent CP-EBUS for peribronchial lesions without endobronchial involvement and adjacent to three-grade bronchi based on chest computed tomography (CT) scan.We recorded the images during EBUS as well as the diagnostic results of TBNA and conventional-transbronchial lung biopsy/brush (C-TBLB/b),and final diagnoses were based on pathologic analysis and follow-up.Results:In all cases,the mass was able to be identified using EBUS in 97.2％ patients (70/72) who were performed with EBUS-TBNA + C-TBLB/b.Sixty-six patients had a final diagnosis,80.0％ patients (56/70) had malignancies,and 14.3％ patients (10/70) had benign disease.In malignancies,the diagnostic yield of C-TBLB/b was 57.1％ (32/56) and in EBUS-TBNA was 85.7％ (48/56),whereas pathologic diagnosis reached 94.6％ when EBUS-TBNA was combined with C-TBLB/b.C-TBLB/b + EBUS-TBNA also exhibited stronger potency ofhistolytic diagnosis for malignancies than either EBUS-TBNA or C-TBLB/b alone.Furthermore,there are data supporting the value of EBUS-TBNA for the diagnosis of benign lung disease.Conclusion:The combined endoscopic approach with EBUS-TBNA and C-TBLB/b is an accurate and effective method for the evaluation of peribronchial lesions,with better results than using each technique alone.

AIMTo investigate the effects of hepatocyte growth factor gene transfected MSCs transplantation on cardiac function and fibrosis in rats heart failure model induced by adriamycin.

METHODSMSCs were isolated from SD rats by density gradient centrifugation, purified, and transfected with Ad-hHGF. ELISA were used to detect the protein expression of hHGF in these MSCs. Forty SD rats underwent intraperitoneal injection with adriamycin to induce heart failure model. 8 healthy rats served as control, 24 survival rats were randomly divided into 3 groups (n = 8): Rats in Ad-hHGF transfected MSCs group were injected with Ad-hHGF transfected MSCs 2 weeks after the establishment of the model, rats in MSC group injected with suspension of MSCs only, and model group was injected with cold culture fluid. Heart function was evaluated by a physiological recorder 4 weeks after cell transplantation. Myocardial cell morphology and interstitial collagen were studied by electron microscope and were stained by Sirus red. TGF-beta1 was detected by immunohistochemical method.

RESULTS(1) MSCs could be transfected efficiently by Ad-hHGF, manifested by a higher level of expression in vitro, persisting 14 days at least. (2) Four weeks after the cells transplantion, cardiac necrosis in MSC-hHGF rats was improved when compared with those in the MSCs (P < 0.05) and Model group (P < 0.01). The heart function of the MSC-hHGF rats was greatly improved with an significant increase in LVSP and + dp/dt(max), although LVEDP still highter than that of normal rats. (3) MSC-Ad-hHGF decreased Myocardial collagen content and the level of TGFbeta1 compaired with MSCs transplanted rats (P < 0.01).

CONCLUSIONTransplantation of HGF gene transfected MSCs improved heart function, decreased myocardial collagen and the level of TGFbeta1.

Animals ; Doxorubicin ; Fibrosis ; metabolism ; prevention & control ; Heart Failure ; chemically induced ; physiopathology ; therapy ; Hepatocyte Growth Factor ; genetics ; Male ; Mesenchymal Stem Cell Transplantation ; methods ; Mesenchymal Stromal Cells ; metabolism ; Myocardium ; pathology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Transfection

OBJECTIVETo investigate the relationship between blood concentration of lactic acid (LA) and tissue oxygenation in severely burned patients with shock.

METHODSThirty-four severely burned patients admitted during early postburn stage were included in this study and were randomly divided into A (n = 18) and B (n = 16) groups. The patients in A group were resuscitated with modified anti-shock programme by which the patients' urine output was maintained roughly around 100ml per hour, while the patients in B group were treated by our traditional resuscitation formula by which the patients urine was kept at 40 ml per hour. The blood concentration of LA and usual indices (urine output, blood pressure, heart rate, and mental status) were simultaneously monitored before and 1, 8, 16, 24, 48 and 72 hours after resuscitation in patients of both groups.

RESULTS(1) The average blood LA level in patients of A group was (3.2 +/- 0.4) mmol/L within 24 hours of resuscitation, while the monitored indices remained within normal range. Nevertheless the LA level in B group was (7.4 +/- 1.6) mmol/L (P < 0.01, compared with that of A group), and hyperlactacidemia lasted for more than 72 hours while other indices were normal. (2) The mortality in B group was high (31.2%), whilst that in A-group was only 5.5% (P < 0.01). (3) There was negative correlation between blood LA and urine output and positive correlation between blood LA and heart rate.

CONCLUSION(1) Blood LA concentration might be taken as an immediate, sensitive, simple and useful index of tissue oxygenation of the whole body during burn shock stage. (2) It was suggested by our results that fluid resuscitation should be extended to 72 PBHs (postburn hours) with urine output over 100 ml/h, so as to ensure the quality and effects of the resuscitation of burn shock.

Adult ; Burns ; metabolism ; physiopathology ; therapy ; Female ; Fluid Therapy ; Humans ; Lactic Acid ; blood ; Male ; Middle Aged ; Oxygen Consumption ; physiology ; Shock, Traumatic ; metabolism ; physiopathology ; therapy ; Young Adult