[Objective] To observe the effects of different fractions from Cortex Eucommiae on oxidation resistance and anti-despondent of Extradiol-induced kidney-yang deficiency mice,and understand the modern scientific connotation of the properties theories about sweet-flavor,warm-nature,meridian tropism in liver and kidney.[Methods] Healthy adult male ICR mice were divided into six groups randomly:normal control group,model control group,positive control group(Guifudihuang),three groups of Cortex Eucommiae(water fraction,n-butanol fraction,and acetoacetate fraction).Kidney-yang deficiency mice model was established with intraperitoneal injection of estradiol benzoate.The determined indexes included oxidation resistance indexes(activity of SOD,GSH-Px,CAT,content of MDA),anti-despondent indexes(tail suspension,activity of MAO),and anti-fatigue ability.[Results] Oxidation resistance indexes:Three fractions of Cortex Eucommiae all could decrease content of MDA,enhance activeness of SOD.The water and n-butanol fraction could enhance activeness of CAT.The water and acetoacetate fraction could enhance activeness of GSH-Px.Anti-despondent indexes:The acetoacetate fraction of Cortex Eucommiae could lengthen the activity time in tail suspension test,and three fractions of Cortex Eucommiae all could decrease activeness of MAO.Three fractions of Cortex Eucommiae all could lengthen the running distance and time.[Conclusion] Cortex Eucommiae could improve aging and depression caused by kidney-yang deficiency.Oxidation resistance and anti-despondent effects could be an important part of the main effective groups about sweet-flavor,warm-nature,meridian tropism in liver and kidney.The effects of water extract and n-butanol extract were closer and stronger,which could be the main substance basis of common effective groups.

Objective To investigate antimicrobial resistance and change trends ofUreaplasma urealyticum (Uu) from reproductive system of female patients at a traditional Chinese medicine hospital in three consecutive years. Methods Antimicrobial resistance of Uu isolated from gynaecology leucorrhea and cervical secretion specimens of patients of dermatology & sexually transmitted diseases department and gynaecology department from January 2011 to December 2013 were analyzed retrospectively.Results A total of 327 Uu strains were isolated,except resistance rates of Uu to doxycycline(5.23%-6.12%)and josamycin(0-1 .96%)remained at a relatively low level in three consecutive years,resistance rate to the other antimicrobials showed an upward trend year by year,but the difference was not significant (P >0.05).The resistance of Uu to ofloxacin,clarithromycin and sparfloxacin were relatively high(42.11 %-61 .40%),to josamycin,doxycycline and minocycline were lower(0-13.07%),and multidrug re-sistance was serious.Conclusion Antimicrobial resistanc of Uu isolated from reproductive system of female patients in this hospital is high.It is necessary to realize the status and change trends of antimicrobial resistance of Uu for the treatment of disease and control of the emergence of multidrug resistance pathogens.

Objective To investigate.the co-stimulatory pathway of gastric cancer patients by examining the expression of co-stimulatory molecules CD28,CTLA.4(CDl 52)and B7 molecules on peripheral blood lymphocytes(PBLC)in patients with gastric cancer,and to discuss the role of costimulatory pathway in the pathogenesis of gastric cancer.Methods The expression of CD28,CD152,CD3,CD4,CD8 and B7 molecules on peripheral blood lymphocytes and T cell subpopulation were measured by flow cytometry in 56 patients with gastric cancer,and the data was compared and analyzed with that in gastric benign tumor group and healthy control group.Results The expression levels of CD3+,CD4+,CD3+ CD28+,B7-1(CD80),B7-2(CD86)and CD41/CD8+on PBLC in patients with gastric cancer was significantly lower than that of benign stomach tumor group and healthy control group.The expression levels of CD3+ CDl52+ and CD8+ on PBLC in patients with gastric cancer were significantly higher than those of gastric benign tumor group and healthy control group.Conclusion Peripheral lymphocytcs in gastric cancer patients express low level of CD28 and B7 molecules and high level of CDI 52 molecules,which results in dysfunction of B7:CD28/CTLA-4 co-stimulation pathway and in turn affects the capacity of T cell to eliminate tumor cells,which makes tumor cells evading the immune surveillance and metastasizing.

OBJECTIVETo observe the efficacy of Xingnaojing Injection (XI) in treatment of sepsis-associated encephalopathy (SAE).

METHODSTotally 65 SAE patients were retrospectively analyzed at EICU from September 2010 to September 2013. They were assigned to the control group (32 cases) and the treatment group (33 cases) according to whether they received XI. Patients in the control group received anti-infection and symptomatic support, while those in the treatment group were intravenously injected with XI at 20 mL per day for additional 7-10 days. The fever clearance time, Glasgow coma scale (GCS), C-reactive protein (CRP), neuron-specific enolase (NSE), and improvement of electroen-cephalogram (EEG) were observed in the two groups.

RESULTSCompared with the control group, the fever clearance time was shortened, CRP levels decreased, GCS score and efficacy of EEG was alleviated in the treatment group after treatment with statistical difference (P < 0.05). No adverse reaction occurred during medication.

CONCLUSIONX1 was safe and effective in treatment of SAE.

C-Reactive Protein ; metabolism ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Injections ; Phosphopyruvate Hydratase ; metabolism ; Sepsis-Associated Encephalopathy ; drug therapy ; Treatment Outcome

BACKGROUND:Fluorescent magnetic nanoparticles with the properties of quantum dots and magnetic particles have good biocompatibility, and can label cels effectively through endocytosis. OBJECTIVE:To validate the feasibility of fluorescent magnetic nanoparticles in labeling human adipose-derived mesenchymal stem cels. METHODS:Healthy human adipose tissue was extracted and adipose-derived mesenchymal stem cels were isolatedin vitro by type I colagenase digestion. Passage 6 cels were incubated with the fluorescent magnetic nanoparticles overnight. Prussian blue staining and laser scanning confocal microscope were used to observe labeled adipose-derived mesenchymal stem celsin vitro after co-culturing with fluorescent magnetic nanoparticles. The tracing effect of labeled adipose-derived mesenchymal stem cels in vivo was detected by fluorescence imaging system. RESULTS AND CONCLUSION:Prussian blue staining showed that the fluorescent magnetic nanoparticles dispersed in the cytoplasm of adipose-derived mesenchymal stem cels in the form of blue particles. Under the laser scanning confocal microscope, the nuclei of adipose-derived mesenchymal stem cels were dyed blue by Hoechest33258, and the cytoplasm was dyed green. The fluorescence imaging results showed that labeled human adipose-derived mesenchymal stem cels had good imaging results. Therefore, as an efficient tracer, the fluorescent magnetic nanoparticles can label human adipose-derived stem cels in vitro and provide a new method for transplantation and transformation of adipose-derived mesenchymal stem cels.

Objective To investigate the MR imaging (MRI) appearances of postoperative residual liver after hepatic resection for hepatocellular carcinoma (HCC) and the MRI features of tumor recurrences.Methods Twenty patients with previous surgical resection of HCC underwent MR examination of upper abdomen for routine follow-up study or due to clinical suspicion of tumor recurrence. MRI protocol included T1W axial unenhanced images and Gadolinium-enhanced sequences, Gadolinium-enhanced VIBE sequence, unenhanced T2W axial images and coronal TrueFisp sequence.Results Thirteen patients showed normal edge of surgical resection, while 6 patients demonstrated MR signs of incision edge recurrence of HCC and 1 patient was suspicious of tumor recurrence at the incision edge. Among the 20 patients, 12 had MRI features of tumor recurrence of the residual liver, including invasion of left, right and common hepatic ducts 3 cases. Three patients had metastatic lymphadenopathy in portal hepatis, portacaval space and retroperitoneal space. Two patients showed extensive tumor implantation of peritoneum and mesentery. Conclusion MRI is effective in differentiating normal surgical incision edge of residual liver from tumor recurrence. It is also very useful for the early detection of intra-hepatic and extra-hepatic tumor lesions.

Objective To compare the load of EB virus in peripheral white blood cell,plasma and serum in the patients with he-matologic diseases,and to discuss the feasibility of detecting EB virus load by using plasma or serum instead of peripheral white blood cell.Methods Venous blood of 125 patients with hematologic diseases were collected,and peripheral white blood cell,plasma and serum were isolated.The real-time quantitative PCR was used to detect the virus load in three kinds samples with the EB virus load in peripheral blood cell as the gold standard.Results Compared to peripheral white blood,the EB virus load in plasma and ser-um showed no statistical difference(P >0.05).Conclusion Using plasma or serum instead of peripheral white blood cell for con-ducting the quantitative detection of the load of EB virus will be a reliable method.

BACKGROUND:Stromal vascular fraction digested from adipose tissue is a mixture of a variety of cel s, and clone-like cel components may have the real ability to maintain the stemness of mesenchymal stem cel s OBJECTIVE:To separate, passage and culture colony-forming cel s in the stromal vascular fraction of adipose tissue, and to test its ability to repair the knee cartilage in animal models. METHODS:Stromal vascular fraction isolated from fresh adipose tissue was cultured to col ect clone-like adherent cel s that were subject to digestion and passage until the third generation. A rabbit model of osteoarthritis was established surgical y, and the third generation of colony-forming cel s were transplantated into the articular cavity of model rabbits. Model rabbits with no cel transplantation served as controls. Cartilage regeneration in the knee joint of model rabbits were observed and compared. RESULTS AND CONCLUSION:At 3 days after transplantation of stromal vascular fraction cel s, clone-like cel colonies appeared in adherent cel s;at 5 days, the amount of clone-like cel colonies and surrounding single cel s were both increased. At the passage 3, the clone-like cel colonies were also visible, which were fusiform in shape. Gross observation and MRI examination showed that these colony-forming cel s could promote cartilage repair in the joint cavity, increase secretion of synovial fluid, and help to control inflammation and narrow ulceration area. Experimental findings are beneficial for optimizing the isolation and culture of adipose-derived mesenchymal stem cel s and provide a new way for the clinical treatment of osteoarthritis.

Objective:To research the application of flurbiprofen compound small dose fentanyl with self-control vein analgesia after laparoscopic cholecystectomy and the influence on blood coagulation function.Methods:102 cases with laparoscopic cholecystectomy who were treated in our hospital from November 2015 to November 2016 were selected and divided into the control group and the research group,with 51 cases in each group.The patients in the control group were treated with postoperative intravenous analgesia with low-dose fentanyl,while the patients in the research group were treated with postoperative intravenous analgesia with flurbiprofen ester compound low-dose fentanyl.Then the fibrinogen (Fg),activated partial prothrombin time (APTT),prothrombin time (PT),platelet count (PLT),substance P,5-hydrocarbon serotonin (5-HT),interleukin 6,8 (IL-6,IL-8) and complications between two groups were observed and compared.Results:Before treatment,there was no statistically significant difference about the Fg,APTT,PT,PLT,substance P,5-HT,IL-6 and IL-8 between two groups (P＞0.05);After treatment,the Fg,APTT,PT,PLT,substance P,5-HT,IL-6 and IL-8 increased in the two groups,while the research group was lower than that of the control group,and the differences were statistically significant (P＜0.05).The postoperative complication rate of research group was lower than that of the control group (P＜0.05).Conclusion:Flurbiprofen ester compound small dose fentanyl with self-control vein analgesia can relieve coagulation function,and inhibit the levels of inflammatory factors.

Animals ; Arginine Vasopressin ; metabolism ; Female ; Hippocampus ; drug effects ; metabolism ; Lead ; toxicity ; Learning ; drug effects ; Memory ; drug effects ; Pregnancy ; Prenatal Exposure Delayed Effects ; Rats ; Rats, Sprague-Dawley