No abstract available.
Free Tissue Flaps*

Duchenne muscular dystrophy (DMD) is the most common and lethal dystrophy in childhood, caused by mutations in the dystrophin (DMD) gene. Multiplex ligation dependent probe amplification (MLPA) or array comparative genome hybridization (aCGH) is widely used as an initial molecular diagnostic tool. If no deletions or duplications are found in MLPA or aCGH, the samples must be subjected to a second test of direct sequencing. Direct sequencing of the DMD gene, however, is time-consuming, high-cost, and can be inconclusive. Here, we performed whole exome sequencing on a patient with progressive muscle weakness whose MLPA result was negative; the result revealed a rare frame shift mutation. Direct sequencing on the patient's mother showed the same mutation. Whole exome sequencing can be a new diagnostic routine for DMD patients with negative MLPA3.
Comparative Genomic Hybridization ; Dystrophin ; Exome* ; Frameshift Mutation ; Genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Mothers ; Multiplex Polymerase Chain Reaction ; Muscle Weakness ; Muscular Dystrophy, Duchenne* ; Pathology, Molecular

Diet is closely related to an economic level, but few studies have reported on the relationship between the economic level and eating habits, especially in college students. Therefore, this study was conducted to clarify differences in eating habits with a focus on processed foods according to allowance level in college students. This study was a cross-sectional survey of 500 college students using a questionnaire. The questionnaire consisted of general characteristics, eating behaviors, purchase of processed foods, and preference and intake frequency of processed foods. All subjects were classified based on monthly allowance: less than Won 300,000 (n=149), Won 300,000~400,000 (n=177), and more than Won 400,000 (n=124). All survey results were comparatively analyzed among the spending money groups. As the level of spending money of the subjects increased, the rate of skipping meals, eating out, and unbalanced diet increased (P<0.05). The reason for consuming processed foods was because they are easy to prepare. The factor considered the most when buying processed foods was price. However, these results showed no significant difference according to level of spending money. As spending money increased preference for retort, convenience, canned, and bottled foods significantly increased. Intake frequency of dairy products was lower, and the frequency of processed foods was significantly higher with more spending money. This study found that a higher level of monthly allowance in college students, was associated with higher rate of skipping meals, eating out, and unbalanced diet, and the preference and intake frequency of processed foods were also high. These results suggest that spending money level in college students, as an economic indicator, is relevant to intake of processed foods.
Chungcheongnam-do* ; Cross-Sectional Studies ; Dairy Products ; Diet ; Eating* ; Feeding Behavior ; Humans ; Meals

PURPOSE: This study aimed to evaluate the clinical features of children who have survived a water submersion incident, and to identify risk factors for prognosis. METHODS: We retrospectively reviewed the medical records of patients who experienced submersion between January 2005 and December 2014. The patients were classified into 2 groups, according to complications, and prognostic factors were evaluated. RESULTS: During the study period, 29 children experienced submersion (20 boys and 9 girls; mean age, 83.8±46.4 months). Submersion occurred most commonly in the summer, with the peak incidence in August. The most frequent Szpilman clinical score was grade 5 (13 patients; 44.8%), followed by grade 6 (7 patients; 24.1%), and grades 1 or 2 (3 patients; 10.3%). Five children (17.2%) in the poor prognosis group died or had hypoxic ischemic encephalopathy, and the overall mortality rate was 6.9%. Poor prognosis after submersion was associated with lower consciousness levels (P=0.003), higher Szpilman scores (P=0.007), greater need for intubation and mechanical ventilator support (P=0.001), and longer duration of oxygen therapy (P=0.015). Poor prognosis was also associated with lower bicarbonate levels (P=0.038), as well as higher sodium, aspartate transaminase (AST), and alanine transaminase (ALT) levels (P=0.034, P=0.006, and P=0.005, respectively). Szpilman clinical scores were positively correlated with consciousness levels (r=0.489, P=0.002) and serum liver enzyme levels (AST and ALT; r=0.521, P=0.004). CONCLUSION: We characterized the prognostic factors associated with submersion outcomes, using the Szpilman clinical score, which is comparable to consciousness level for predicting mortality.
Alanine Transaminase ; Aspartate Aminotransferases ; Child* ; Consciousness ; Drowning* ; Female ; Humans ; Hypoxia-Ischemia, Brain ; Immersion ; Incidence ; Intubation ; Liver ; Medical Records ; Mortality ; Oxygen ; Prognosis ; Retrospective Studies ; Risk Factors ; Sodium ; Ventilators, Mechanical ; Water

Testosterone is required for the development and maintenance of the male accessory sex organs and their normal function. And it was reported that castration affect cells in the adult male rat accessory sex glands by induction of programmed cell death (apoptosis). So, in this study, the authors made an experiment to evaluate the effect of testosterone in the maure male rat penis and accessory sex glands following castration. Also, we utilized actinomycin D, a potent inhibitor of messenger and ribosomal RNA synthesis, in the experiment herein to assess the significance of regression process in the glands. Following are the changes in the serum testosterone level, the weight of the penis, ventral prostate and seminal vesicles and apoptosis occurrence of the control (castration, castration normal saline) and experimental (castration AD25, castration AD50) group of mature rats. 1. After castration, the control group and the experimental group showed decreased level of serum testosterone. 2. In the both groups, the weight of the penis, ventral prostate and seminal vesicles decreased gradually. 3. Compared to the control group, the castration AD25 did not show the inhibition of castration induced regression of penis and ventral prostate. However, castration AD50 showed the inhibition. 4 In the H-E staining and ApoTag in situ staining, the ventral prostate showed the most prominent apoptosis occurrence followed by the seminal vesicles and penis. These results suggest that after castration of the mature rat, due to testosterone deficiency, the weight of penis, ventral prostate and seminal vesicles decreased with the occurrence of apoptosis. Also, actinomycin D 50 micrometer seems to delay the regression process.
Adult ; Animals ; Apoptosis ; Castration* ; Cell Death ; Dactinomycin ; Genitalia ; Humans ; Male ; Penis* ; Prostate ; Rats* ; RNA, Ribosomal ; Seminal Vesicles ; Testosterone*

OBJECTIVES: The purpose of this study was to investigate the eating behavior toward processed foods among college students who live in different types of residence. METHODS: This is a cross-sectional study targeting a total of 476 college students living at home with their family, living in a rental house with self-boarding, living in a lodging house, and living in a dormitory. Eating behaviors, including preference and the frequency of processed food intake were surveyed and compared according to the type of residence. RESULTS: The rate of skipping a meal was significantly higher among students who reported self-boarding than those living in other types of residences. The main reason for skipping meals was that they got up late. In the entire study population, the main reason for consuming processed food was easy-to-cook (33.8%) and the primary consideration for choosing processed food was the price (54.0%). The processed food the most favored by college students was the processed noodles; those living at home with their family or living in a dormitory preferred milk products; those living in a rental house with self-boarding or in a lodging house preferred confectionery, retort pouch, convenience food, and canned/bottled food. The frequency of processed food intake was significantly higher in the students who reported self-boarding than those living in other types of residences (p < 0.001). CONCLUSIONS: Students' preference toward processed foods differed according to their type of residence. The frequency of processed food intake was significantly higher in students who reported self-boarding indicating that the type of residence of student is associated with their choices and consumption of processed foods.
Cross-Sectional Studies ; Eating* ; Fast Foods ; Feeding Behavior ; Humans ; Korea ; Meals ; Milk

No abstract available.
Mucocele*

No abstract available.
Cartilage* ; Fascia* ; Transplants* ; Tympanoplasty*

Purpose: The purpose of this study was to investigate the sleep duration, social jetlag (SJL), and subjective sleep disturbance according to the individual chronotype in rotating shift nurses. Methods: A total of 344 rotating 3-shift nurses (mean age 28.67 years) were recruited at one university affiliated hospital. They completed the following questionnaires, which were used to assess their chronotype and sleep: the morningness-eveningness questionnaire (MEQ), self-reported sleep duration of work days (SDW shift) and free days (SDF shift) in each shift (day [D], evening [E], night [N]), and sleep disturbance (Insomnia severity index, ISI). SJL shift was calculated as the difference in midsleep (MS = sleep onset+1/2 sleep duration) between work days (MSW) and free days (MSF). Results: Subjects were divided into 3 chronotype groups according to the MEQ; morning (MG, 4.4%) intermediate (IG, 57.8%), and evening groups (EG, 37.8%). SDW D was shortest (4.68 hr) and SDF E was longest (8.93 hr) in the EG. SJL D was longest in the EG (3.77 hr), and SJL N was longest in the MG (7.37 hr). The prevalence of sleep disturbance was 33.3% (MG), 29.6% (IG), and 40.0% (EG), respectively, without any statistical significance. Conclusion In order to improve the sleep of shift workers, it is recommended that the evening chronotypes should reduce the day shifts and the morning chronotypes should reduce the night shifts. We also propose a study to determine whether these shift assignments can improve the sleep in shift nurses.

Purpose: The purpose of this study was to investigate the sleep duration, social jetlag (SJL), and subjective sleep disturbance according to the individual chronotype in rotating shift nurses. Methods: A total of 344 rotating 3-shift nurses (mean age 28.67 years) were recruited at one university affiliated hospital. They completed the following questionnaires, which were used to assess their chronotype and sleep: the morningness-eveningness questionnaire (MEQ), self-reported sleep duration of work days (SDW shift) and free days (SDF shift) in each shift (day [D], evening [E], night [N]), and sleep disturbance (Insomnia severity index, ISI). SJL shift was calculated as the difference in midsleep (MS = sleep onset+1/2 sleep duration) between work days (MSW) and free days (MSF). Results: Subjects were divided into 3 chronotype groups according to the MEQ; morning (MG, 4.4%) intermediate (IG, 57.8%), and evening groups (EG, 37.8%). SDW D was shortest (4.68 hr) and SDF E was longest (8.93 hr) in the EG. SJL D was longest in the EG (3.77 hr), and SJL N was longest in the MG (7.37 hr). The prevalence of sleep disturbance was 33.3% (MG), 29.6% (IG), and 40.0% (EG), respectively, without any statistical significance. Conclusion In order to improve the sleep of shift workers, it is recommended that the evening chronotypes should reduce the day shifts and the morning chronotypes should reduce the night shifts. We also propose a study to determine whether these shift assignments can improve the sleep in shift nurses.